Cell Lines &amp; Specialty Cell Culture

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

HLA DRB1: 1201 1401
HLA DRB3: 0101 0202
HLA DQAP: 1.3 4.1
HLA DQA1: 0104 0501
HLA DQB1: 0301 0503
HLA DPB1: 02012 0501

Serological Profile

HLA A: 2 33
HLA A ief: 2.2
HLA C: 3 4
HLA B: 35 61

RPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

biological source	human blood
ethnicity	Not specified
gender	female
growth mode	Suspension
karyotype	Not specified
morphology	Lymphoblastoid
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice

Homozygous: No
Consanguineous: No

RIDADR	NONH for all modes of transport
WGK Germany	3

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YI human cell line

A: 2602 2402
Cw: 0303 1202
B: 5201 4002
HLA DRB1: 1405 0901
HLA DQB1: 05031 0303
HLA DPB1: 0201 0501

Serological Profile

HLA A: 24 26
HLA A ief:
HLA C: 9
HLA B: 52 61
HLA DR: 9 14
HLA DR52: 52
HLA DR53: 53
HLA DQ: 1 3

RPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

biological source	human blood
ethnicity	Japanese
gender	not specified
growth mode	Suspension
karyotype	Not specified
morphology	Lymphoblastoid
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice

Rat Y3Ag1.2.3 x Rat LOU spleen

RIDADR	NONH for all modes of transport
WGK Germany	3

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YL1/2

The antibody recognises tubulin from yeast as well as from birds and mammals. It appears to be specific for the α subunit of tubulin.

Yeast tubulin

DMEM (D6546), + 2mM L-Glutamine (G7513), + 10% FBS (F2442),

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

biological source	Rat x Rat Hybridoma
ImmunoDon	LOU rat spleen
Myeloma	Y3Ag1.2.3
Reactivity	Anti-Tubulin
application(s)	cell culture \| mammalian: suitable
isotype	IgG2a
shipped in	dry ice
storage temp.	−196°C

Rat Y3Ag1.2.3 x Rat F344 spleen

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YN1/1.7.4

The antibody detects MALA-2 found primarily on activated T and B lymphocytes. The antigen has a molecular weight of 95-100kDa and is the murine equivalent of the human intercellular adhesion molecule ICAM-I. antibody directly inhibits proliferating cell populations in MLR.

NS1 cells

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Rat x Rat Hybridoma spleen
ImmunoDon	F344 rat spleen
Myeloma	Y3Ag1.2.3
Reactivity	Anti-Murine Activated Lymphocyte Antigen-2 (MALA-2)
application(s)	cell culture \| mammalian: suitable
isotype	IgG2b
shipped in	dry ice
storage temp.	−196°C

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The full designation of this line is YB2/3.0Ag30. YO was derived from a hybrid myeloma YB2/3HL by cloning in soft agar to select a non-secreting sub-population. The cells are azaguanine resistant and do not secrete IgG.

Hybridisation and cloning studies

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	rat blood
growth mode	Suspension
karyotype	Not specified
morphology	Not specified
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

Rat YB2/O x Rat LOU spleen

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YOL1/34

The antibody recognises tubulin from yeast as well as from birds and mammals. It appears to be specific for the α subunit of tubulin.

Yeast tubulin

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Rat x Rat Hybridoma
ImmunoDon	LOU rat spleen
Myeloma	YB2/O
Reactivity	Anti-Tubulin
application(s)	cell culture \| mammalian: suitable
isotype	IgG2a
shipped in	dry ice
storage temp.	−196°C

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YTA 3.1.2

The antibody is directed against epitope B of the L3T4 antigen. This antigen is the murine equivalent of CD4. Has been used in CD4 subset depletion in vivo and flow cytometry studies. Cambridge Enterprise Reference number AB13:www.enterprise.cam.ac.uk.

CBA mouse spleen cells, thymocytes

Not specified

Iscoves Modified Dulbeccos Medium (IMDM) + 2 mM glutamine + 5% Fetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA, trypsin or mechanical agitation maybe required to dislodge them from the plastic.

Maintain cultures between 3-9 x 105 cells/ml; 5% CO2; 37 °C. Saturation density 9 x 105/ml

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Rat x Rat Hybridoma
ImmunoDon	DA rat spleen
Myeloma	Y3Ag1.2.3
Reactivity	Anti-L3T4 epitope B, mouse CD4
application(s)	cell culture \| mammalian: suitable
isotype	IgG2b
shipped in	dry ice
storage temp.	−196°C

Monoclonal antibody human CD25

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YTA 74.4.4

Rat hybridoma producing antibody against mouse CD7. Cambridge Enterprise Reference number AB110:www.enterprise.cam.ac.uk.

Activated CBA mouse splenic T cells

IDMEM, (I3390) + 2 mM L-Glutamine, (G7513) + 5% FBS / FCS, (F2442). Where the cells are grown under static conditions, 0.02% EDTA, Trypsin, (T4424) or mechanical agitation maybe required to dislodge them from the plastic.

Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Rat x Rat Hybridoma
ImmunoDon	DA rat spleen
Myeloma	Y3.AG.1.2.3
Reactivity	Mouse transferrin receptor (CD71)
application(s)	cell culture \| mammalian: suitable
isotype	IgG2a
shipped in	dry ice

Monoclonal antibody human CD45R

RIDADR	NONH for all modes of transport
WGK Germany	3

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YTH 30.15

Кат. номер

11060807-RNA-5UG

11060807-1VL

11060807-DNA-5UG

Cell Line Origin
Monoclonal antibody human CD28
Cell Line Description
Rat hybridoma producing antibody against human CD7. Cambridge Enterprise Reference number AB69:www.enterprise.cam.ac.uk.
Immunogen
Human peripheral blood mononuclear cells
Culture Medium
IDMEM, (I3390) + 2 mM L-Glutamine, (G7513) + 5% FBS / FCS, (F2442). Where the cells are grown under static conditions, 0.02% EDTA, Trypsin, (T4424) or mechanical agitation maybe required to dislodge them from the plastic.
Subculture Routine
Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Параметры

biological source Rat x Rat Hybridoma

ImmunoDon DA rat spleen

Myeloma Y3.AG.1.2.3

Reactivity Human CD7

application(s) cell culture | mammalian: suitable

isotype IgG2b

shipped in dry ice

Safety Information

RIDADR NONH for all modes of transport

WGK Germany 3

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YTH 470.5

Rat hybridoma producing antibody against human beta 2 microglobulin. Cambridge Enterprise Reference number AB81:www.enterprise.cam.ac.uk.

Activated human T cells

Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Rat x Rat Hybridoma
ImmunoDon	DA rat spleen
Myeloma	Y3.AG.1.2.3
Reactivity	Anti Human HLA beta 2 microglobulin
application(s)	cell culture \| mammalian: suitable
isotype	IgG2b
shipped in	dry ice

Monoclonal antibody human CD45: Leukocyte Common Antigen (T200)

RIDADR	NONH for all modes of transport
WGK Germany	3

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YTH 53.1

Rat hybridoma producing antibody against human CD59. Cambridge Enterprise Reference number AB114:www.enterprise.cam.ac.uk.

Human peripheral blood mononuclear cells

Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Rat x Rat Hybridoma
ImmunoDon	DA rat spleen
Myeloma	Y3.AG.1.2.3
Reactivity	Human homologous complement restriction factor (CD59)
application(s)	cell culture \| mammalian: suitable
isotype	IgG2b
shipped in	dry ice

Monoclonal antibody Human CD59 Homologous complement restriction factor

RIDADR	NONH for all modes of transport
WGK Germany	3

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YTH 71.3

Rat hybridoma producing antibody against human CD66 (human carcinoembryonic antigen CEA). Cambridge Enterprise Reference number AB64:www.enterprise.cam.ac.uk.

Human peripheral blood mononuclear cells

Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Rat x Rat Hybridoma
ImmunoDon	DA rat spleen
Myeloma	Y3.AG.1.2.3
Reactivity	Anti Human carcinoembryonic antigen (CEA)(CD66)
application(s)	cell culture \| mammalian: suitable
isotype	IgG2a
shipped in	dry ice

Monoclonal antibody human leukocyte antigen class 1 (HLA-1)

RIDADR	NONH for all modes of transport
WGK Germany	3

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YTH 81.5

Rat hybridoma producing antibody against human CD11a. Cambridge Enterprise Reference number AB82:www.enterprise.cam.ac.uk.

Human peripheral blood mononuclear cells

Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Rat x Rat Hybridoma
ImmunoDon	DA rat spleen
Myeloma	Y3.AG.1.2.3
Reactivity	Anti Human LFA1 (integrin alpha L CD11a)
application(s)	cell culture \| mammalian: suitable
isotype	IgG2a
shipped in	dry ice

Monoclonal antibody, mouse CD4 epitope A

RIDADR	NONH for all modes of transport
WGK Germany	3

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YTH 906.9HL

Rat hybridoma producing antibody against human CD25. The designation HL indicates that it has bselected for loss of the original Y3 myeloma derived (non-specific) light chain. Cambridge Enterprise Reference number AB61:www.enterprise.cam.ac.uk.

Activated human T cells

Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Rat x Rat Hybridoma
ImmunoDon	DA rat spleen
Myeloma	Y3.AG.1.2.3
Reactivity	Human IL2 receptor alpha (CD25)
application(s)	cell culture \| mammalian: suitable
isotype	IgG2b
shipped in	dry ice

Monoclonal antibody mouse CD90 (Thy-1)

RIDADR	NONH for all modes of transport
WGK Germany	3

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YTS 154.7.7.10

The antibody is directed against the monomorphic determinant of the mouse Thy-1 T cell antigen. Has been used in T+NK cell depletion in vivo and flow cytometry studies. Cambridge Enterprise Reference number AB10:www.enterprise.cam.ac.uk.

RPMI 1640 + 2mM glutamine + 0.05mM 2-mercaptoethanol (2ME) + 20% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37°C. Saturation density 9 x 105/ml

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Rat x Rat Hybridoma
ImmunoDon	DA rat spleen
Myeloma	Y3Ag1.2.3
Reactivity	Anti-Thy-1, mouse
application(s)	cell culture \| mammalian: suitable
isotype	IgG2b
shipped in	dry ice
storage temp.	−196°C

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YTS 156.7.7

The antibody is directed against the B epitope of the mouse Lyt-3 (CD8) antigen.Has been used in CD8 subset depletion in vivo and flow cytometry studies. Cambridge Enterprise Reference number AB15: www.enterprise.cam.ac.uk.

DMEM + 2 mM glutamine + 0.05 mM 2-mercaptoethanol (2ME) + 20% Fetal Bovine Serum (FBS)

Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Rat x Rat Hybridoma
ImmunoDon	DA rat spleen
Myeloma	Y3Ag1.2.3
Reactivity	Anti-Lyt-3 epitope B, mouse
application(s)	cell culture \| mammalian: suitable
isotype	IgG2b
shipped in	dry ice
storage temp.	−196°C

Monoclonal antibody mouse CD8 (lyt-2)

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YTS 169.4.2.1

The antibody is directed against the A epitope of the mouse Lyt-2 T cell (CD8) antigen.Cambridge Enterprise Reference number AB14:www.enterprise.cam.ac.uk.

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37°C. Saturation density 9 x 105/ml

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Rat x Rat Hybridoma
ImmunoDon	DA rat spleen
Myeloma	Y3Ag1.2.3
Reactivity	Anti-Lyt-2, mouse
application(s)	cell culture \| mammalian: suitable
isotype	IgG2b
shipped in	dry ice
storage temp.	−196°C

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YTS 177.9.6.1

Кат. номер

11060819-DNA-5UG

11060819-1VL

11060819-RNA-5UG

Cell Line Origin
Monoclonal antibody human CD18
Cell Line Description
Rat hybridoma producing antibody against mouse CD4 epitope A. Cambridge Enterprise Reference number AB111:www.enterprise.cam.ac.uk.
Immunogen
CBA mouse spleen cells, thymocytes
Application
Rat hybridoma producing antibody against mouse CD4 epitope A
Culture Medium
IDMEM, (I3390) + 2 mM L-Glutamine, (G7513) + 5% FBS / FCS, (F2442). Where the cells are grown under static conditions, 0.02% EDTA, Trypsin, (T4424) or mechanical agitation maybe required to dislodge them from the plastic.
Subculture Routine
Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Параметры

biological source Rat x Rat Hybridoma

ImmunoDon DA rat spleen

Myeloma Y3.AG.1.2.3

Reactivity Anti- L3T4 epitope B, mouse CD4

application(s) cell culture | mammalian: suitable

shipped in dry ice

Safety Information

RIDADR NONH for all modes of transport

WGK Germany 3

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YTS 191.1.1.2

The antibody is directed against the A epitope of the mouse L3T4 T cell antigen, the murine equivalent of CD4. Has been used in in vivo depleting and flow cytometry studies. Cambridge Enterprise Reference number AB16: www.enterprise.cam.ac.uk.

RPMI 1640 + 2 mM glutamine + 0.05 mM 2-mercaptoethanol (2ME) + 20% Fetal Bovine Serum (FBS)

Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Rat x Rat Hybridoma
ImmunoDon	DA rat spleen
Myeloma	Y3Ag1.2.3
Reactivity	Anti-L3T4, mouse
application(s)	cell culture \| mammalian: suitable
isotype	IgG2b
shipped in	dry ice
storage temp.	−196°C

Monoclonal antibody human beta 2-microglobulin

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YTS 213.1.1

Кат. номер

11060803-DNA-5UG

11060803-1VL

11060803-RNA-5UG

Cell Line Origin
Monoclonal antibody human carcinoembryonic antigen CEA CD66
Cell Line Description
Rat hybridoma producing antibody against mouse CD18. Cambridge Enterprise Reference number AB109:www.enterprise.cam.ac.uk.
Immunogen
CBA mouse spleen cells, thymocytes
Culture Medium
IDMEM, (I3390) + 2 mM L-Glutamine, (G7513) + 5% FBS / FCS, (F2442). Where the cells are grown under static conditions, 0.02% EDTA, Trypsin, (T4424) or mechanical agitation maybe required to dislodge them from the plastic.
Subculture Routine
Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Параметры

biological source Rat x Rat Hybridoma

ImmunoDon DA rat spleen

Myeloma Y3.AG.1.2.3

Reactivity Mouse beta 2 integrin (CD18)

application(s) cell culture | mammalian: suitable

isotype IgG2a

shipped in dry ice

Safety Information

RIDADR NONH for all modes of transport

WGK Germany 3

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YW 62.3.20

Rat hybridoma producing antibody against mouse CD45 pan isoform reactive. Cambridge Enterprise Reference number AB62:www.enterprise.cam.ac.uk.

CBA mouse bone marrow

Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Rat x Rat Hybridoma
ImmunoDon	DA rat spleen
Myeloma	Y3.AG.1.2.3
Reactivity	Anti mouse CD45 pan isoform reactive
application(s)	cell culture \| mammalian: suitable
isotype	IgG2b
shipped in	dry ice

RIDADR	NONH for all modes of transport
WGK Germany	3

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The immunisation protocol involved repeated intranasal infection with a single i.v. injection before fusion. Produces IgG2b antibody which binds murine IgG2a of "a" and "b" allotype, Fc region. There is no cross-reactivity with A/PR/8/34 influenza virus, DNP, NP, DNA or cytoskeletal proteins.

Differential detection of complexed IgG2a in the presence of native IgG2a, allotype detection or quantitation.

RPMI 1640 or DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS) (can be adapted to 1% Foetal Bovine Serum (FBS))

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	Sp2/0
Reactivity	Anti-IgG2a, mouse
application(s)	cell culture \| mammalian: suitable
isotype	IgG2b
shipped in	dry ice
storage temp.	−196°C

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Z26

The immunisation protocol involved repeated intranasal infection with human influenza virus and a single i.v. injection before fusion. Produces IgM which binds murine IgG2a of "a" allotype, Fc region. There is no cross-reactivity with A/PR/8/34 influenza virus, DNP, NP, DNA or cytoskeletal proteins. Contains a CH2 domain.

Differential detection of complexed IgG2a in the presence of native IgG2a, allotype detection or quantitation.

RPMI 1640 or DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS) (can be adapted to 1% Foetal Bovine Serum (FBS))

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	Sp2/0
Reactivity	Anti-IgG2a, mouse
application(s)	cell culture \| mammalian: suitable
isotype	IgM
shipped in	dry ice
storage temp.	−196°C

RIDADR	NONH for all modes of transport
WGK Germany	3

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Z34

The immunisation protocol involved repeated intranasal infection and a single i.v. injection before fusion. The antibody binds murine IgG2a of "a" and "b" allotype, Fc region. The isoallotype-specific Z34 IgA shows cross-reactivity with IgG2b ("b" allotype) of C57BL/6 origin but not of BALB/c origin. There is no cross-reaction with A/PR/8/34 influenza virus, DNP, NP, DNA or cytoskeletal proteins. Contains a CH2 domain.

Differential detection of complexed IgG2a in the presence of native IgG2a, allotype detection or quantitation.

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS) (can be adapted to 1% Foetal Bovine Serum (FBS))

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	Sp2/0
Reactivity	Anti-IgG2a and IgG2b (allotype dependent), mouse
application(s)	cell culture \| mammalian: suitable
isotype	IgA
shipped in	dry ice
storage temp.	−196°C

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Z41

The immunisation protocol involved repeated intranasal infection with a single i.v. injection before fusion. The IgM antibodies bind murine IgG2a of "a" allotype and Fc region. There is no cross-reactivity with A/PR/8/34 influenza virus, DNP, NP, DNA or cytoskeletal proteins. Contains a CH2 domain.

Differential detection of complexed IgG2a in the presence of native IgG2a, allotype detection or quantitation.

RPMI 1640 or DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS) (can be adapted to 1% Foetal Bovine Serum (FBS))

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	Sp2/0
Reactivity	Anti-IgG2a, mouse
application(s)	cell culture \| mammalian: suitable
isotype	IgM
shipped in	dry ice
storage temp.	−196°C

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

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ZL34

Кат. номер

11120713-1VL

11120713-CDNA-20UL

11120713-RNA-5UG

11120713-DNA-5UG

Cell Line Origin
A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure
Cell Line Description
ZL34 was derived from a fibrous tumour of the pleural cavity in a 53-year old male patient, prior to treatment, with a known history of exposure to asbestos.
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 11,13
D13S317: 11,12
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 6
TPOX: 8
vWA: 18,20
Culture Medium
DMEM:F12 (1:1), (D8062) + L-Glutamine, (G7513) + 15% FBS / FCS, (F2442).
Subculture Routine
Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37 °C. Suggested seeding density 1-4 x 10,000 cells/cm2.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Параметры

biological source human lung

growth mode Adherent

karyotype Not specified

morphology Mesenchymal with thin cytoplasmic processes

receptors Not specified

relevant disease(s) cancer

Safety Information

RIDADR NONH for all modes of transport

WGK Germany 3

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ZL5

ZL5 was derived from the pleural effusion of a 43-year old male patient, prior to treatment, with a known history of exposure to asbestos.

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,13
D16S539: 12
D5S818: 12
D7S820: 10,11
THO1: 6,9.3
TPOX: 8,11
vWA: 16,17

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS)

Split subconfluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin/EDTA; 5% CO2; 37 °C. Suggested seeding density 2-4x10,000 cells/cm. The cells in suspension should be collected by centrifugation and added to the trypsinized cells before reseeding.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	human lung (effusion)
growth mode	Adherent
karyotype	Modal
morphology	Spheroidal
products	Not specified
receptors	Mesothelial-membrane antigen ME1
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice

Homozygous: No
Consanguineous: No

RIDADR	NONH for all modes of transport
WGK Germany	3

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ZL55 cell line human

Кат. номер

12050301-DNA-5UG

12050301-RNA-5UG

12050301-1VL

12050301-CDNA-20UL

Cell Line Description
ZL55 was derived from an epithelial tumor of the pleural cavity of a 52-year old male, prior to treatment, with a known history of exposure to asbestos.
DNA Profile
STR-PCR Data:

Amelogenin: X
CSF1PO: 9,11
D13S317: 8
D16S539: 12
D5S818: 10,13
D7S820: 7,11
THO1: 6
TPOX: 8
vWA: 15,19
Culture Medium
DMEM:HAMS F12 (1:1) + 2mM Glutamine + 15% Fetal Bovine Serum (FBS)
Subculture Routine
Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Suggested seeding density 1-4 x 10,000 cells/cm.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Параметры

biological source human pleural cavity

growth mode adherent

morphology Epitheloid/mesenchymal

shipped in dry ice

storage temp. −196°C

Safety Information

RIDADR NONH for all modes of transport

WGK Germany 3

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ZM-FC human cell line

HLA DRB1: 0403 11
HLA DQA1: 03 0501
HLA DQB1: 0304 0301

Serological Profile

Not specified

RPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

biological source	human blood
ethnicity	Sardinian Caucasian
gender	not specified
growth mode	Suspension
karyotype	Not specified
morphology	Lymphoblastoid
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice

Human Caucasian breast carcinoma

RIDADR	NONH for all modes of transport
WGK Germany	3

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ZR-75-1

Derived from a malignant ascitic effusion in a 63 year old female Caucasian with infiltrating ductal carcinoma. The cells are reported to express both the wildtype and variant oestrogen receptors, progesterone receptor and other steroid hormones.

ZR-75-1 has been used to determine the effects of cucurbitacin E (CuE) extracted from Ecballium elaterium A.Rich., Fam. Cucurbitaceae.
Hormone and tumourigenicity studies

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,11
D13S317: 9
D16S539: 11
D5S818: 13
D7S820: 10,11
THO1: 7,9.3
TPOX: 8
vWA: 16,18

RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells grow very slowly, do not form a confluent monolayer and attach only lightly to the substrate. After subculture, it can take up to 7 days before cells re-attach. Detachment of cells can occur in PBS and trypsinisation can produce clumps of cells. To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. When subculturing, ensure the trypsin is completely removed by centrifuging the cells and resuspending them in fresh medium.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	human breast
growth mode	Adherent
karyotype	2n = 46, modal no. 72
morphology	Epithelial
products	Not specified
receptors	Reported to express the oestrogen and progesterone receptors
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	196°C

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ZR-75-30

Human breast carcinoma

Derived from ascitic fluid of a 47 year old woman with infiltrating ductal carcinoma. Ethnicity: Black.

Tumourigenicity studies

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 10,11
D16S539: 9
D5S818: 12,13
D7S820: 11,12
THO1: 8,9
TPOX: 10,11
vWA: 18,19

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. Cells produce a significant amount of cellular debris appearing as black particles. This cell line grows relatively slowly and requires passaging approximately every 5 days.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	human breast
growth mode	Adherent
karyotype	Not specified
morphology	Epithelial
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

Thalidomide is a stereoisomer, which exists in two enantiomeric states. The S enantiomer is teratogenic. Thalidomide consists of two linked rings, a phthalimide and glutarimide ring.

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()-Thalidomide

Empirical Formula (Hill Notation):	C₁₃H₁₀N₂O₄
CAS Number:	841-67-8
Molecular Weight:	258.23
MDL number:	MFCD00210219
PubChem Substance ID:	24899969
NACRES:	NA.77

Thalidomide has been used to study its teratogenic effects in chicken embryos and human embryonic cells. This study reported that thalidomide causes limb defects by stabilizing PTEN, inhibiting the expression of Akt and activating caspase-dependent apoptosis. Thalidomide has also been used for studying glutathione mediated teratogenic resistance in mouse embryos.

10 mg in poly bottle
25, 100 mg in glass bottle

(-)-Thalidomide selectively inhibits the biosynthesis of tumor necrosis factor α (TNF-α), which is essential for inflammatory response. It is an anti-emetic drug and is used to treat morning sickness in pregnant women. Thalidomide is also used to treat leprosy, multiple myeloma, Crohn′s disease and human immunodeficiency virus (HIV) infection. Thalidomide also inhibits angiogenesis. It is associated with several diseases such as, peripheral neuropathy, facial palsies, Duane syndrome and autism.

Features and Benefits

This compound was developed by Celgene,. To browse the list of other pharma-developed compounds and Approved Drugs/Drug Candidates, click here,.

Preparation Note

(-)-Thalidomide is soluble in DMSO, but is insoluble in water and ethanol.

assay	>98%
form	solid
optical activity	[]23/D 62.6°, c = 2 in DMF(lit.)
application(s)	cell culture \| embryo: suitable
color	white
solubility	DMSO: soluble, H₂O: insoluble, ethanol: insoluble
originator	Celgene
SMILES string	O=C1CC[C@H](N2C(=O)c3ccccc3C2=O)C(=O)N1
InChI	1S/C13H10N2O4/c16-10-6-5-9(11(17)14-10)15-12(18)7-3-1-2-4-8(7)13(15)19/h1-4,9H,5-6H2,(H,14,16,17)/t9-/m0/s1
InChI key	UEJJHQNACJXSKW-VIFPVBQESA-N
Gene Information	human ... LITAF(9516), TNF(7124) mouse ... Nos2(18126) rat ... Nos1(24598)

Select the right albumin for your application with the Albumin Selection Guide
This albumin has been qualified for use in media designed to support in vitro applications such as mouse blastocyst and morulae culture.

pictograms	GHS07,GHS08
signalword	Danger
hcodes	H302 - H360
pcodes	P201 - P301 + P312 + P330 - P308 + P313
Personal Protective Equipment	Eyeshields,, Gloves,, type P1 (EN143) respirator filter,, type P3 (EN 143) respirator cartridges,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3

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Bovine Serum Albumin

CAS Number:	9048-46-8
EC Number:	232-936-2
MDL number:	MFCD00130384
NACRES:	NA.75

10, 50, 100 g in poly bottle

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Quality Level	200
biological source	bovine
product line	BioXtra
assay	≥98% (agarose gel electrophoresis)
form	powder
mol wt	~66 kDa
packaging	poly bottle of
application(s)	cell culture \| embryo: suitable
loss	≤5%
pH	6.5-7.5
solubility	water: soluble (40 mg/ml)
shipped in	ambient
storage temp.	2-8°C

This glucose has been qualified for use in mouse embryo in vitro work.

Personal Protective Equipment	Eyeshields, Gloves, type N95 (US)
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

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D-(+)-Glucose

Empirical Formula (Hill Notation):	C₆H₁₂O₆
CAS Number:	50-99-7
Molecular Weight:	180.16
Beilstein/REAXYS Number:	1724615
EC Number:	200-075-1
MDL number:	MFCD00063774
PubChem Substance ID:	24895248
NACRES:	NA.75

1 kg in poly bottle
100, 500 g in poly bottle

Mixed anomers

Quality Level	200
assay	99.5% (GC)
form	powder
application(s)	cell culture \| embryo: suitable
mp	150-152 °C (lit.)
solubility	H₂O: 2g + 15 mL, clear, colorless
SMILES string	OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@@H]1O
InChI	1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6+/m1/s1
InChI key	WQZGKKKJIJFFOK-DVKNGEFBSA-N
Gene Information	human ... PYGM(5837)

Tryptophan is one of the essential and standard amino acids present in the body. It is used by the cells to produce proteins.

Personal Protective Equipment	Eyeshields, Gloves, type N95 (US)
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

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D-Tryptophan

Empirical Formula (Hill Notation):	C₁₁H₁₂N₂O₂
CAS Number:	153-94-6
Molecular Weight:	204.23
Beilstein/REAXYS Number:	86198
EC Number:	205-819-9
MDL number:	MFCD00005647
PubChem Substance ID:	24900598
NACRES:	NA.32

D-Tryptophan has been used to study its efficacy on dispersing biofilm in dental unit waterlines (DUWLs) in vitro.
D-Tryptophan can be used for Human embryonic kidney cell (HEK-293, ATCC: CRL-1573) culture.The product is a sweetener used to study the release of incretins from enteroendocrine cells triggered by sugar but not by sweeteners.

1, 5 g in glass bottle
100 mg in poly bottle
25 g in poly bottle
100 g in glass bottle

Low level of D-tryptophan is capable of preventing the growth of an L-tryptophan-requiring mutant of the E. coli.

Unnatural isomer of tryptophan.
Tandem Mass Spectrometry data independently generated by Scripps Center for Metabolomics is available to view or download in PDF. T9753.pdf Tested metabolites are featured on Scripps Center for Metabolomics METLIN Metabolite Database. To learn more, visit sigma.com/metlin.

Quality Level	200
biological source	synthetic (organic)
assay	98.0% (HPLC)
form	powder
application(s)	cell culture \| embryo: suitable, cell culture \| mammalian: suitable
color	white to off-white
mp	282-285 °C (dec.) (lit.)
SMILES string	N[C@H](Cc1c[nH]c2ccccc12)C(O)=O
InChI	1S/C11H12N2O2/c12-9(11(14)15)5-7-6-13-10-4-2-1-3-8(7)10/h1-4,6,9,13H,5,12H2,(H,14,15)/t9-/m1/s1
InChI key	QIVBCDIJIAJPQS-SECBINFHSA-N
Gene Information	human ... GRINA(2907) mouse ... GRINA(66168) rat ... GRINA(266668)

Personal Protective Equipment	Eyeshields, Gloves, type N95 (US)
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

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E-4031

Empirical Formula (Hill Notation):	C₂₁H₂₇N₃O₃S · 2HCl
CAS Number:	113559-13-0
Molecular Weight:	474.44
MDL number:	MFCD01754739
PubChem Substance ID:	329818000
NACRES:	NA.77

E-4031 has been used as:

human ether-a-go-go-related gene (hERG) blocker in human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)
IKr blocker in long QT syndrome (LQTS) induced pluripotent stem (iPSCs) embryoid bodies
IKr blocker in rat ventricular myocytes

0.1, 0.5, 1 mg in glass bottle

E-4031 is a antiarrhythmic drug and belongs to the class III type. It is a methanesulfonanilide compound and is effective in treating arrhythmia and modulates ventricular fibrillation. E-4031 mediates the prolongation of action potential duration (APD) in transgenic long-QT type 1 (LQT1) rabbits. An isoleucine mutation in human ether-a-go-go-related gene (hERG) abolishes its interaction with E-4031.
E-4031 selectively blocks hERG K+ channels.

Features and Benefits

This compound is featured on the Potassium Channels, page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here,.

Quality Level	100,
assay	98% (HPLC)
form	lyophilized powder
storage condition	desiccated
application(s)	cell culture \| embryo: suitable
color	white
solubility	H₂O: soluble
storage temp.	20°C
SMILES string	Cl.Cl.Cc1cccc(CCN2CCC(CC2)C(=O)c3ccc(NS(C)(=O)=O)cc3)n1
InChI	1S/C21H27N3O3S.2ClH/c1-16-4-3-5-19(22-16)12-15-24-13-10-18(11-14-24)21(25)17-6-8-20(9-7-17)23-28(2,26)27;;/h3-9,18,23H,10-15H2,1-2H3;2*1H
InChI key	ZQBNWMFBOSOOLX-UHFFFAOYSA-N

This solution is designed for the removal of Zonae pellucidae from mouse oocytes.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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EmbryoMax™ Acidic Tyrodes Solution MR-004-D

eCl@ss:	32160801
NACRES:	NA.75

General description

Store at -20°C upto 2 months & 1 day

EmbryoMax is a trademark of Merck KGaA, Darmstadt, Germany

EmbryoMax Advanced KSOM Medium is a new, uniquely buffered modified version of KSOM that can be used as a single medium to harvest and culture preimplantation mouse embryos. The medium will maintain pH out on the bench top and also grow embryos in vitro, replacing the normal 2-step system where one medium is used for embryo harvest and another is used for culture. The Advanced KSOM medium has been shown to produce a higher frequency of blastocyst formation in multiple mouse strains after 72 hours in culture when compared to standard KSOM medium.
Traditionally, retrieving and then growing mouse embryos in vitro is usually a 2-step system. Embryos are commonly removed from the oviducts in a HEPES buffered medium (e.g. M2, FHM), which maintain their pH in air. The embryos are then transferred into a second medium which is bicarbonate buffered (e.g. M16, CZB, KSOM) and placed in a CO2 incubator in order to maintain the pH of the medium. Furthermore, the bicarbonate buffered medium needs to be pre-equilibrated in the incubator for several hours prior to receiving embryos to achieve optimal growth. The EmbryoMax Advanced KSOM Medium enables both embryo handling in atmospheric conditions and culturing in a CO2 incubator with a single medium. No medium pre-equilibration step is necessary for embryo harvest, but pre-equilibration is recommended for culture.

Quality Level	300,
form	liquid
Торговая марка	EmbryoMax^™, Specialty Media
application(s)	cell culture \| embryo: suitable, cell culture \| stem cell: suitable
input	sample type: mouse embryo(s)

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EmbryoMax™ Advanced KSOM Embryo Medium MR-101-D

eCl@ss:

32160801

General description

Research Category
Stem Cell Research
EmbryoMax Advanced KSOM Medium can be used as a single medium to harvest and culture preimplantation mouse embryos, replacing the normal 2-step system where one medium is used for embryo harvest and another is used for culture.

Can be stored at -20°C up to the expiration date on the bottle.
Once thawed it should be used within 2 weeks.
Note: Our EmbryoMax Liquid Mouse Embryo Media is produced on a bi-monthly basis. For regular users, we encourage standing orders to ensure that backorders do not occur.

EmbryoMax is a trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	300
form	liquid
Торговая марка	EmbryoMax^™, Specialty Media
application(s)	cell culture \| embryo: suitable, cell culture \| stem cell: suitable
input	sample type: mouse embryo(s)

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EmbryoMax™ FHM Mouse Embryo Media MR-025-D

eCl@ss:	32160801
NACRES:	NA.71

Store at -20°C upto 2 months & 1 day.

Note: Our EmbryoMax Liquid Mouse Embryo Media is produced on a bi-monthly basis. For regular users, we encourage standing orders to ensure that backorders do not occur

EmbryoMax is a trademark of Merck KGaA, Darmstadt, Germany

Quality Level	300,
form	liquid
Торговая марка	EmbryoMax^™, Specialty Media
application(s)	cell culture \| embryo: suitable, cell culture \| stem cell: suitable
input	sample type: mouse embryo(s)

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EmbryoMax™ FHM Mouse Embryo Media MR-122-D

eCl@ss:	32160801
NACRES:	NA.71

EmbryoMax is a trademark of Merck KGaA, Darmstadt, Germany

Quality Level	300,
form	liquid
Торговая марка	EmbryoMax^™, Specialty Media
application(s)	cell culture \| embryo: suitable, cell culture \| stem cell: suitable
input	sample type: mouse embryo(s)

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EmbryoMax™ FHM Mouse Embryo Media MR-024-D

eCl@ss:	32160801
NACRES:	NA.71

Store at -20°C upto 2 months & 1 day

Note: Our EmbryoMax Liquid Mouse Embryo Media is produced on a bi-monthly basis. For regular users, we encourage standing orders to ensure that backorders do not occur.

EmbryoMax is a trademark of Merck KGaA, Darmstadt, Germany

Quality Level	300,
form	liquid
Торговая марка	EmbryoMax^™, Specialty Media
application(s)	cell culture \| embryo: suitable, cell culture \| stem cell: suitable
input	sample type: mouse embryo(s)

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EmbryoMax™ Human Tubal Fluid (HTF) (1X), liquid, for Mouse IVF MR-070-D

eCl@ss:	32160801
NACRES:	NA.75

Can be stored at -20°C upto the expiration date on the bottle.
Once thawed it should be used within 2 weeks.

Note: Our EmbryoMax Liquid Mouse Embryo Media is produced on a bi-monthly basis. For regular users, we encourage standing orders to ensure that backorders do not occur.

EmbryoMax is a trademark of Merck KGaA, Darmstadt, Germany