Cell Lines &amp; Specialty Cell Culture

The antibody is directed against a 188kDa polypeptide present on the basolateral plasma membrane of Madin-Darby canine kidney (MDCK) cells. This polypeptide is the same as, or closely related to, the cell adhesion molecule uvomorulin.

Madin-Darby canine kidney (MDCK) cells

Study of epithelial occluding barriers

EMEM(EBSS) + 2mM glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	P3X63Ag8.653
Reactivity	Anti-Madin-Darby canine kidney cell E-cadherin (uvomorulin)
application(s)	cell culture \| mammalian: suitable
isotype	IgG1, kappa
shipped in	dry ice
storage temp.	−196°C

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Hamster Chinese ovary

Overproduces ornithine decarboxylase in response to polyamine deprivation.

Enzymatic studies

EMEM (EBSS) + 2mM Glutamine + Ham′s F12 (without Putrescine) (1:1) + 0.1% BSA.

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using cold PBS and 0.25% cold trypsin. Place flasks at 4°C for 5-10 minutes or until cells detach. Culture cells at 37°C; 5% CO2. Pre-soak flasks in porcine

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biological source	hamster ovary
growth mode	Adherent
karyotype	Not specified
morphology	Epithelial
products	Ornithine decarboxylase
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

Human caucasian metastatic melanoma

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A2058

This cell line has been isolated from a lymph node metastasis from a 43 year old male patient with malignant melanoma. Due to their highly invasive properties, the cells can be used as a source of cellular invasion associated proteins e.g. collagen type IV collagenase, tissue inhibitor of metalloproteinase-2 and autocrine motility factor.

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

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biological source	human skin (lymph node metastasis)
growth mode	Adherent
karyotype	Not specified
morphology	Epithelial
products	Not specified
receptors	Nerve growth factor (NGF), Laminin
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	metastasis
shipped in	dry ice
storage temp.	−196°C

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A2780 Cell Line human

Human ovarian carcinoma

The A2780 human ovarian cancer cell line was established from tumour tissue from an untreated patient. Cells grow as a monolayer and in suspension in spinner cultures. A2780 is the parent line to the cisplatin resistant cell line A2780 cis (Sigma catalogue no. 93112517) and the adriamycin resistant cell line A2780 ADR (Sigma catalogue no. 93112520).

A2780 cell line human is used in toxicity testing and cancer genetic studies.
A2780 cell line human has been used to:

study the role of Snail and Slug in ovarian tumor neovascularization
overcome cisplatin resistance in ovarian cancer cells using liposome application
demonstrate the significance of NMDA (N-methyl-D-aspartate) receptors as targets for treatment of ovarian cancers
study the effect of low molecular weight heparin tinzaparin on cisplatin resistance in ovarian cancer cells

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 12,13
D16S539: 11,13
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,10
vWA: 15,16

RPMI-1640, + 2mM Glutamine, + 10% Foetal Boving Serum, (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 3-6x10,000cells/cm2 using 0.25% Trypsin, or Trypsin/EDTA,; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

biological source	human ovary
growth mode	Adherent
karyotype	Not specified
morphology	Epithelial
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

Human Caucasian ovarian carcinoma

RIDADR	NONH for all modes of transport
WGK Germany	3

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A2780ADR Cell Line human

The adriamycin-resistant cell line A2780ADR has been developed by exposure of the parent A2780 cell line (Sigma catalogue no. 93112519) to adriamycin. A2780ADR is cross-resistant to melphalan and vinblastine. To retain resistance adriamycin has to be added to the media. The cells grow as a monolayer and in suspension in spinner cultures and are tumourigenic in immune deficient mice. Together with the cisplatin-resistant variant A2780cis these lines only differ in their exposure to a single drug and should facilitate the search for molecular changes responsible for the expression of pleiotropic drug resistance in human ovarian cancer.

Study of drug transport, toxicity testing, drug resistance cancer studies

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 11,14
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,9
vWA: 15,16

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS); treatment with 10E-7 M adriamycin at least once a week.

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Recommendation: culture cells without drug after resuscitation until growth has been fully established.

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Disclaimer

biological source	human ovary
description	Human Caucasian ovarian carcinoma
growth mode	Adherent
karyotype	Not specified
morphology	Epithelial
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

RIDADR	NONH for all modes of transport
WGK Germany	3

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A2780cis Cell Line human

Human ovarian carcinoma

This cisplatin-resistant cell line has been developed by chronic exposure of the parent cisplatin-sensitive A2780 cell line (Sigma catalogue no. 93112519) to increasing concentrations of cisplatin. A2780cis is cross-resistant to melphalan, adriamycin and irradiation. An increased ability to repair DNA damage as well as cytogenetic abnormalities has been observed. In order to retain resistance cisplatinum has to be added to the media every 2-3 passages. In addition to this matched pair of drug-sensitive/resistant cell lines an adriamycin-resistant cell line, A2780adr (Sigma catalogue no. 93112520), has been isolated from the same parental line A2780.

Toxicity testing, drug resistance studies
2780cis human cell line has been used to study the cytotoxic effects of platinum (II) complexes and gold (I)-triphenylphosphine complexes with hypoxanthine-derived ligands. It has also been used to study the effects of cancer-cell specific oligopeptides.

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 11,13
D5S818: 11
D7S820: 10
THO1: 6
TPOX: 8,10
vWA: 15,16

RPMI 1640,+ 2 mM Glutamine, + 1 µm Cisplatin, + 10% Fetal Bovine Serum FBS, (cisplatinum only necessary every 2-3 passages).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x10,000cells/cm using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells will attach slowly after resuscitation and take up to 7 days to reach confluency. Recommendation: resuscitate cells in media without cisplatin. Add after subculture of attached cells.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Legal Information

Adriamycin is a registered trademark of RDF Pharmacia & Upjohn S.P.A.

Disclaimer

biological source	human ovary
growth mode	Adherent
karyotype	Modal no. 46
morphology	Epithelial
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

Mouse P3X63Ag8 x Mouse BALB/c spleen

RIDADR	NONH for all modes of transport
WGK Germany	3

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A2B5 clone 105

This cytotoxic antibody is directed against a glycolipoid present on chick embryo retinal neuron cell bodies. The antigen is found in chick embryo brain, spinal cord and dorsal root ganglia neurons and in bovine and human brain. The antigen is not present on axons or dendrites.

Chicken embryo retina cells

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

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biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	P3X63Ag8
Reactivity	Anti-Retinal Neuron Glycolipid, chick
application(s)	cell culture \| mammalian: suitable
isotype	IgM
shipped in	dry ice
storage temp.	−196°C

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A2H

Hamster Chinese ovary

Used for the induction and isolation of nutritionally deficient mutants. A reclone of ′Clone A′ established by Puck (the ′H′ denotes MRC Harwell). Useful for genetic studies due to the low chromosome number.

Genetic studies

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

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biological source	hamster ovary
growth mode	Adherent
karyotype	2n = 22
morphology	Epithelial
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

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A375 Cell Line human

Human malignant melanoma

Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.

A375 cell line has been used to obtain paracrine factors for prolonged culture of mesenchymal stromal cells (MSCs). It has also been used to study oncolytic activity of the peptide LTX-315.
Tumourigenicity studies

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.

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biological source	human skin
description	Human malignant melanoma
growth mode	Adherent
karyotype	Modal no. = 62 (hypotriploid) 9 marker chromosomes. Normal N2, N6 and N22 present.
morphology	Epithelial
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

Human malignant melanoma IL-1 insensitive

RIDADR	NONH for all modes of transport
WGK Germany	3

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A375-C5 Cell Line human

The cell line A375-C5 is unresponsive to the anti-proliferative effect of IL-1. It has been derived from the parental line A373 (Sigma catalogue no. 88113005) which was subjected to cloning by limiting dilution. This method resulted in the establishment of A375-C5 and an IL-1 sensitive clone, A375-C6 (Sigma catalogue no. 97011321). The unresponsiveness of A375-C5 is probably due to an IL-1 receptor deficiency. The cells have been eradicated from mycoplasma at ECACC.

IL-1 resistance studies, receptor studies

RPMI 1640 + 2mM Glutamine + 10% FBS

Split sub-confluent cultures (70-80%) 1:5 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	human skin
description	Human malignant melanoma IL-1 insensitive
growth mode	Adherent
karyotype	Not specified
morphology	Epithelial-like
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

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A431 Cell Line human

Human squamous carcinoma

Derived from an epidermal carcinoma of the vulva taken from an 85 yr old female. The cells carry large numbers of EGF binding sites and is an indicator cell for anti-TGF binding.

Cell cycle studies
A431 Cell Line human has been used:

to demonstrate that p38 mitogen-activated protein kinase enhances epidermal growth factor-induced apoptosis in A431 cells
to study melanoma-specific CD44 (a tumor marker and transmembrane receptor) alternative splicing pattern in A431 cells
to study the anti-cancer activity of antioxidant-rich Cameroonian medicinal plants

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	human skin
growth mode	Adherent
karyotype	Hypertriploid
morphology	Epithelial
products	Not specified
receptors	Epidermal growth factor (EGF)
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

Human Caucasian lung carcinoma

RIDADR	NONH for all modes of transport
WGK Germany	3

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A549 Cell Line human

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

Enzymatic studies
Human A549 cell line has been used:

to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions
to study the effects of insulin and insulin-like growth factor 1 (IGF1) on proliferation and apoptosis in A549 cells
to culture A549 cells in different media over a long term and identify conditions for promoting alveolar type II differentiation characteristics in A549 cells

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14

Ham′s F12K + 2mM Glutamine, + 10% Foetal Bovine Serum FBS / FCS,.

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin, or tTrypsin/EDTA,; 5% CO2; 37°C.

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biological source	human lung (carcinoma)
growth mode	Adherent
karyotype	Hypotriploid
morphology	Epithelial
products	Lecithin, High amounts of de-saturated fatty acids.
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

South African clawed toad kidney

RIDADR	NONH for all modes of transport
WGK Germany	3

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Derived from normal kidneys of an adult male toad (Xenopus laevis). A6 cells support the replication of Granoff′s frog viruses but not the Lucke inclusion-tumour associated virus.

Replication studies

NCTC 109 + 15% Distilled H2O + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 27-28°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	toad kidney
growth mode	Adherent
karyotype	Hyperdiploid
morphology	Epithelial
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

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A673

Derived from a 15 year old female patient. The cells form colonies on soft agar and tumours in antithymocyte serum treated mice.
The cell line A673 was originally described in 1973 as derived from a rhabdomyosarcoma, however, more recent analysis of the cells using advanced diagnostic techniques determined the cells to have characteristics of Ewing sarcoma rather than rhabdomyosarcoma. See citations for more information about this tumour status.

Tumourigenicity studies

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 8,13
D16S539: 11
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 15,18

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	human muscle
growth mode	Adherent
karyotype	Near Diploid
morphology	Epithelial-like
products	Transforming growth factor (Tramnsforming Growth Factor (TGF)) -
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

Rat BDIX smooth muscle embryonic aorta

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A7r5 Cell Line from rat

Flat ribbon-like cells differentiating to parallel arrays of spindle shaped cells. Derived from the thoracic aorta of an embryonic BDIX rat. As cells achieve the stationary phase there is an increase in myokinase and creatine phosphokinase (CPK) activity. After cessation of cell division a muscle type CPK isoenzyme is synthesised.

Biochemical and biophysical studies
A7r5 Cell Line from rat has been used to study the effect of angiotensin II on vascular oxidative stress. It has been used in PLA2 inhibitory study to understand the role of PLA2 on formation of lipid droplets.

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. After resuscitation cells may take up to 7 days before a monolayer forms and growth can be very slow in general. Cells also grow in clumps.

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biological source	rat muscle
description	Rat BDIX smooth muscle embryonic aorta
growth mode	Adherent
karyotype	Not specified
morphology	Fibroblast
products	Muscle-type isoenzymes
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

Mouse C3H/An areolar and adipose tissue

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Derivative of L929 selected in azaguanine (3μg/ml). Partially resistant to azaguanine. A9 may be heterozygous for the ability to synthesise active inosinic acid phosphorylase.

Metabolic studies

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-5x10,000/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

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biological source	mouse adipose tissue
growth mode	Adherent
karyotype	Aneuploid
morphology	Fibroblast
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

Mouse Fibroblast (L cells)

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A9(Hamprecht)

A 6-thioguanine resistant mutant of mouse L cell fibroblasts (Mouse strain C3H/An). A9 lacks guanylic acid-inosinic acid pyrophosphorylase and was isolated by virtue of resistance to 6-thioguanine (3 μg/ml). ECACC has two catalogue entries for the A9 cell line which differ in that they were deposited by different groups: A9 (Sigma Catalogue number 85011426) and A9(Hamprecht) (Sigma Catalogue number 08062526). Several other cell lines in the ECACC catalogue have been derived from A9(Hamprecht).

Used in the generation of cell hybrids.

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.

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biological source	mouse connective tissue
growth mode	Adherent
karyotype	Modal No of chromosomes is 54
morphology	Fibroblast
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

A9 tumour induced C3H mouse

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A9HT

This cell line was selected by passaging A9 (Sigma Catalogue number. 85011426) through syngenic C3H or semi allogenic mice over a period of three months and uptake was considerably higher than A9, thus A9 high uptake or A9HT. Karyotype is similar to A9 but with a slightly reduced total number of chromosomes, with the modal number of 53. This line lacks inosinic acid pyrophosphorylase.

Tumourigenicity studies

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

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biological source	mouse unknown/unspecified
growth mode	Adherent
karyotype	Hyperdiploid
morphology	Fibroblast
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

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AC2

Derived from a mouse basophil clone and is dependent on Interleukin-3 (IL-3) for growth. IL-3 may be added in the form of Wehi conditioned media (10-20%). Culture densities between 10,000 and 100,000 cells/ml is necessary to retain cells in exponential growth phase.

Growth factor studies, IL-2 assays

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Maintain cultures between 10,000 to 100,000 cells/ml; 5% CO2; 37°C. Cells will rapidly die at high densities above 5x100,000 cells/ml. Culture very sensitive to agitation.

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biological source	mouse blood
growth mode	Suspension
karyotype	Not specified
morphology	Lymphoblast
products	Not specified
receptors	Interleukin 3 (IL-3)
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

Human renal adenocarcinoma

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ACHN

Derived from the pleural effusion of a 22 year old male patient with metastatic renal adenocarcinoma. The cells have been reported to be growth inhibited by human interferons.

Interferon and interferon-induction studies

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	human kidney
growth mode	Adherent
karyotype	Not specified
morphology	Fibroblast
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	metastasis
shipped in	dry ice
storage temp.	−196°C

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ADL, HG human cell line

Кат. номер

94082244-DNA-5UG

94082244-CDNA-20UL

94082244-RNA-5UG

94082244-1VL

General description
Homozygous: No
Consanguineous: No
Cell Line Description
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA Profile
A: 01 68
B: 08 40
HLA DRB1: 0301 0401
HLA DRB3: 101
HLA DRB4: 101
HLA DQAP: 3.1 4.1
HLA DQA1: 0501 03
HLA DQB1: 0201 0301
HLA DPB1: 0101 0301
Serological Profile
HLA A: 1 28
HLA A ief:
HLA C: 3
HLA B: 8 60
Culture Medium
RPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Subculture Routine
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
Параметры

biological source human blood

ethnicity Caucasian

gender male

growth mode Suspension

karyotype Not specified

morphology Lymphoblastoid

application(s) cell culture | mammalian: suitable

shipped in dry ice

Safety Information

RIDADR NONH for all modes of transport

WGK Germany 3

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Aedes albopictus cell line 90100401-1VL

Cell Line Origin

Mosquito larvae

Derived from pooled newly hatched Aedes albopictus larvae. The cells are susceptible to a range of Mosquito borne viruses.

Virus studies: Mosquito viruses

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 3x1,000 to 2x10,000 cells/cm2using

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Quality Level	100,
biological source	mosquito larvae
growth mode	Adherent
karyotype	Diploid
morphology	Epithelial
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

Horse peripheral blood lymphocytes x Mouse NS0

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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AFRC 17D1

The heterohybridoma AFRC 17D1 was obtained by fusion of lymphocytes from ponies immunised with the influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)) with mouse myeloma NS0 cells. The hybridoma was made aminopterin-sensitive by selective growth in 8-azaguanine. This non-Ig-secreting cell line was used as fusion partner for the establishment of equine-mouse heterohybidomas.

Fusion partner for hybridoma production

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Horse x Mouse/Horse Hybridoma
ImmunoDon	Horse peripheral lymphocytes
Myeloma	NS0
Reactivity	Application: Fusion partner for hybridoma production
application(s)	cell culture \| mammalian: suitable
isotype	Not specified
shipped in	dry ice
storage temp.	−196°C

Horse peripheral lymph node x AFRC 17D1

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AFRC 56D2

The heterohybridoma AFRC 56D2 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)). For the establishment of AFRC 56D2 horse cells from the peripheral lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).

Haemagglutinin detection
The isotype of the monoclonal antibody produced by this cell line is horse IgG.

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Horse x Mouse/Horse Hybridoma
ImmunoDon	Horse peripheral lymph node
Myeloma	AFRC 17D1
Reactivity	Anti-Haemagglutinin, influenza
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

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AFRC 58D6

The heterohybridoma AFRC 58D6 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)). For the establishment of AFRC 58D6 horse cells from the sub-mandibular lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).

Haemagglutinin detection
The isotype of the monoclonal antibody produced by this cell line is horse IgG.

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Horse x Mouse/Horse Hybridoma
ImmunoDon	Horse sub-mandibular lymph node
Myeloma	AFRC 17D1
Reactivity	Anti-Haemagglutinin, influenza
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

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AFRC 78A3

The heterohybridoma AFRC 78A3 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)). For the establishment of AFRC 78A3 horse cells from the sub-mandibular lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).

Haemagglutinin detection
The isotype of the monoclonal antibody produced by this cell line is horse IgG.

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Horse x Mouse/Horse Hybridoma
ImmunoDon	Horse sub-mandibular lymph node
Myeloma	AFRC 17D1
Reactivity	Anti-Haemagglutinin, influenza
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

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AFRC 84B4

The heterohybridoma AFRC 84B4 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)). For the establishment of AFRC 84B4 horse cells from the sub-mandibular lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).

Haemagglutinin detection
The isotype of the monoclonal antibody produced by this cell line is horse IgG.

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Horse x Mouse/Horse Hybridoma
ImmunoDon	Horse sub-mandibular lymph node
Myeloma	AFRC 17D1
Reactivity	Anti-Haemagglutinin, influenza
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

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AFRC 84C6 94120830-1VL

Cell Line Origin

The heterohybridoma AFRC 84C6 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)). For the establishment of AFRC 84C6 horse cells from the sub-mandibular lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).

Haemagglutinin detection
The isotype of the monoclonal antibody produced by this cell line is horse IgG.

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Horse x Mouse/Horse Hybridoma
ImmunoDon	Horse sub-mandibular lymph node
Myeloma	AFRC 17D1
Reactivity	Anti-Haemagglutinin, influenza
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

Horse inguinal lymph node x AFRC 17D1

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AFRC 85A2

The heterohybridoma AFRC 85A2 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79(H3N8)). For the establishment of AFRC 85A2 horse cells from the inguinal lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).

Haemagglutinin detection
The isotype of the monoclonal antibody produced by this cell line is horse IgG.

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Horse x Mouse/Horse Hybridoma
ImmunoDon	Horse inguinal lymph node
Myeloma	AFRC 17D1
Reactivity	Anti-Haemagglutinin, influenza
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

Horse inguinal lymph node x AFRC 17D1

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AFRC 85B5

The heterohybridoma AFRC 85B5 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79(H3N8)). For the establishment of AFRC 85B5 horse cells from the inguinal lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).

Haemagglutinin detection
The isotype of the monoclonal antibody produced by this cell line is horse IgG.

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Horse x Mouse/Horse Hybridoma
ImmunoDon	Horse inguinal lymph node
Myeloma	AFRC 17D1
Reactivity	Anti-Haemagglutinin, influenza
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

Horse inguinal lymph node x AFRC 17D1

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AFRC 85D6

The heterohybridoma AFRC 85D6 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79(H3N8)). For the establishment of AFRC 85D6 horse cells from the inguinal lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).

Haemagglutinin detection
The isotype of the monoclonal antibody produced by this cell line is horse IgG.

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Horse x Mouse/Horse Hybridoma
ImmunoDon	Horse inguinal lymph node
Myeloma	AFRC 17D1
Reactivity	Anti-Haemagglutinin, influenza
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

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AFRC IAH-CC1

The antibody is directed against all lymphoid cells in primary and secondary lymphoid tissues. It is considered to be directed against the leukocyte common antigen in bovine CD45.

Bovine thoracic duct lymphocytes

Flow cytometry, immunohistology.

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 10,000 to 100,000 cells/ml; 5% CO2; 37°C. May need macrophage feeder layer.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	NS1
Reactivity	Anti-CD45, bovine
application(s)	cell culture \| mammalian: suitable
isotype	IgG1
shipped in	dry ice
storage temp.	−196°C

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AFRC IAH-CC13

The antibody is directed against a thymic differentiation antigen in cattle. The antigen is expressed on 50-60% of bovine thymocytes, but not on peripheral blood mononuclear cells. The antigen recognised is the bovine equivalent of CD1.

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	NS1
Reactivity	Anti-CD1, bovine
application(s)	cell culture \| mammalian: suitable
isotype	IgG3
shipped in	dry ice
storage temp.	−196°C

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AFRC IAH-CC14

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	NS1
Reactivity	Anti-CD1, bovine
application(s)	cell culture \| mammalian: suitable
isotype	IgG1
shipped in	dry ice
storage temp.	−196°C

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AFRC IAH-CC15

The antibody is directed against WC1 antigen found on bovine CD4- CD8- and CD2- T cells which express gamma/delta TcR. The antigen has a molecular weight of 215-300kDa and is equivalent to the T19 antigen in sheep. The antibody is directed against equivalent antigen in sheep and goats.

Bovine lymphocytes

Flow cytometry, immunohistology, immunoprecipitation, complement lysis, in vivo depletion

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	NS1
Reactivity	Anti-WC1, bovine
application(s)	cell culture \| mammalian: suitable
isotype	IgG2a
shipped in	dry ice
storage temp.	196°C

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AFRC IAH-CC17

The antibody is directed against cells of T cell lineage paracortex of bovine lymph nodes and cells in both the cortex and medulla of thymus. The antigen has a molecular weight of approximately 67kDa and is identified as BoCD5, the bovine equivalent of CD5.

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	NS1
Reactivity	Anti-CD5, bovine
application(s)	cell culture \| mammalian: suitable
isotype	IgG1
shipped in	dry ice
storage temp.	−196°C

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AFRC IAH-CC20

The antibody is directed against the BoCD1 antigen, the bovine equivalent of CD1. The antibody will also react with the equivalent antigen in sheep and goats.

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	NS1
Reactivity	Anti-CD1, bovine
application(s)	cell culture \| mammalian: suitable
isotype	IgG2a
shipped in	dry ice
storage temp.	−196°C

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AFRC IAH-CC21

The antibody is directed against the WC3 antigen present on bovine B cells. The antigen has a molecular weight of 145kDa. The antibody will also react with the equivalent antigen in sheep and goats.

Bovine macrophages

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	NS1
Reactivity	Anti-WC3, bovine
application(s)	cell culture \| mammalian: suitable
isotype	IgG1
shipped in	dry ice
storage temp.	−196°C

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AFRC IAH-CC29

The antibody is directed against cells of T cell lineage paracortex of bovine lymph nodes, and cells in both the cortex and medulla of thymus. The antigen has a molecular weight of approximately 67kDa and is identified as BoCD5, the bovine equivalent of CD5.

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	NS1
Reactivity	Anti-CD5, bovine
application(s)	cell culture \| mammalian: suitable
isotype	IgG1
shipped in	dry ice
storage temp.	−196°C

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AFRC IAH-CC30

The antibody is directed against the BoCD4 antigen, which is the bovine equivalent of CD4.

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	NS1
Reactivity	Anti-CD4, bovine
application(s)	cell culture \| mammalian: suitable
isotype	IgG1
shipped in	dry ice
storage temp.	−196°C

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AFRC IAH-CC37

The antibody is directed against a 145kDa antigen on bovine B cells identified as BoWC3.

Bovine macrophages

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	NS1
Reactivity	Anti-WC3, bovine
application(s)	cell culture \| mammalian: suitable
isotype	IgG1
shipped in	dry ice
storage temp.	−196°C

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AFRC IAH-CC38

The antibody is directed against the BoCD6 antigen, which is the bovine equivalent of CD6.

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	NS1
Reactivity	Anti-CD6, bovine
application(s)	cell culture \| mammalian: suitable
isotype	IgG2b
shipped in	dry ice
storage temp.	−196°C

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AFRC IAH-CC39

The antibody is directed against the WC1 antigen, a 215 - 300kDa molecule found on CD4- CD8- CD2- T cells which express the gamma/delta T cell receptor (TCR). This antigen is equivalent to the T19 antigen in sheep. The antibody will react with the equivalent antigen in sheep and goats.

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	NS1
Reactivity	Anti-WC1, bovine
application(s)	cell culture \| mammalian: suitable
isotype	IgG1
shipped in	dry ice
storage temp.	−196°C

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AFRC IAH-CC42

The antibody is directed against the BoCD2 antigen which is the bovine equivalent of CD2.

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	NS1
Reactivity	Anti-CD2, bovine
application(s)	cell culture \| mammalian: suitable
isotype	IgG1
shipped in	dry ice
storage temp.	−196°C

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AFRC IAH-CC51

The antibody is directed against the WC3 antigen present on bovine B cells. The antigen has a molecular weight of 145kDa.

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	NS1
Reactivity	Anti-WC3, bovine
application(s)	cell culture \| mammalian: suitable
isotype	IgG2b
shipped in	dry ice
storage temp.	−196°C

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AFRC IAH-CC55

The antibody identifies the WC4 antigen, a 90kDa molecule on bovine B cells.

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	NS1
Reactivity	Anti-WC4, bovine
application(s)	cell culture \| mammalian: suitable
isotype	IgG1
shipped in	dry ice
storage temp.	−196°C

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AFRC IAH-CC56

The antibody is directed against a 180kDa molecule present on bovine B cells.

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	NS1
Reactivity	Anti-B cells, bovine
application(s)	cell culture \| mammalian: suitable
isotype	IgG2a
shipped in	dry ice
storage temp.	−196°C

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AFRC IAH-CC57

The antibody is directed against the WC4 antigen on bovine B cells. The antigen has a molecular weight of 90kDa. The antibody will recognises the equivalent antigen in sheep and goats.

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	NS1
Reactivity	Anti-WC4, bovine
application(s)	cell culture \| mammalian: suitable
isotype	IgG1
shipped in	dry ice
storage temp.	−196°C