Cell Lines & Specialty Cell Culture
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FM-95
Кат. номер 13012425-1VL 13012425-CDNA-20UL 13012425-RNA-5UG 13012425-DNA-5UG Cell Line DescriptionFM-95 (ESTDAB-034) was established from a metastatic cutaneous malignant melanoma of a male patient. The FM-95 cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commissions fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB, contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation.DNA ProfileSTR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,10
D16S539: 9,13
D5S818: 12,13
D7S820: 8,11
THO1: 9.3,9.3
TPOX: 8
vWA: 15,18Culture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) seed new cultures at 2-4 x10,000 cells/cm using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density approx 8 x10,000 cells/cmOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply, for more information.Параметрыbiological source human skin description Human melanoma, metastasis growth mode Adherent morphology Fibroblastic application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C Узнать цену -
FM3A
Кат. номер 87100804-DNA-5UG 87100804-1VL 87100804-RNA-5UG Cell Line OriginMouse C3H mammary carcinomaCell Line DescriptionA temperature sensitive mutant of the C3H mouse mammary carcinoma.ApplicationTumourigenicity studiesCulture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 1-3x100,000 cells/ml; 5% CO2; 30-33°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse breast growth mode Suspension karyotype Not specified morphology Lymphoblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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FM3Ats C1.T85
Кат. номер 87111904-1VL 87111904-DNA-5UG 87111904-RNA-5UG Cell Line OriginMouse C3H mammary carcinomaCell Line DescriptionA temperature sensitive mutant isolated from the mouse mammary cell carcinoma cell line, FM3A (Sigma Catalogue number. 87100804). Cells will not divide at 39°C (non-permissive temperature) and will grow at 33°C (permissive temperature). Electron microscope findings have shown that chromatin abnormally condenses into fragmented and compact forms in 80% of the cells at 39°C for 16 hours. This abnormal condensation does not occur at 33°C or in the FM3A cell line at either temperature.ApplicationTumourigenicity studies, chromosome condensation studies.Culture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 1-3x100,000 cells/ml; 5% CO2; 33°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse breast growth mode Suspension karyotype Not specified morphology Lymphoblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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FoLu
Кат. номер 90120501-DNA-5UG 90120501-RNA-5UG 90120501-1VL Cell Line OriginFox Grey lungCell Line DescriptionEstablished from normal lung tissue of an adult female grey fox (Urocyon cinereoargenteus). The cells are susceptible to vesicular stomatitis, herpes simplex and vaccinia viruses.ApplicationVirus studies: VSV, HSV and Vaccinia virusCulture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source fox lung growth mode Adherent karyotype 2n = 66, diploid morphology Fibroblast-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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FPAF human cell line
Кат. номер 95051624-CDNA-20UL 95051624-1VL 95051624-RNA-5UG 95051624-DNA-5UG General descriptionHomozygous: Yes
Consanguineous: YesCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 101
Cw: 401
B: 3502
HLA DRB1: 11041
HLA DRB3: 202
HLA DQA1: 103
HLA DQB1: 603
HLA DPA1: 2
HLA DPB1: 201Serological ProfileHLA A: 1
HLA A ief: 1
HLA C: 4
HLA B: 35
HLA B ief: 35.2
Bw4/bw6: 6
HLA Dw: FS
HLA DR: 11
HLA DR52: 52
HLA DQ: 6
HLA DP: 2Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Ashkenasi Jewish gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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FR
Кат. номер 87020503-1VL 87020503-RNA-5UG 87020503-DNA-5UG Cell Line OriginRat Sprague-Dawley skinCell Line DescriptionEstablished from a skin biopsy of a foetal (18 day gestation) germ-free Sprague-Dawley rat. The cells can be propagated for at least 30 passages.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat skin growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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FS6-14.13 Ag2.1 cell line 85082303-1VL
Cell Line DescriptionThis cell line is a stable T cell hybridoma which, when stimulated with concanavalin A, produces IL-2. The cell surface markers include Thy1.2 and H-2b products.Culture MediumRPMI 1640 + 2 mM glutamine + 1 mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS).Subculture RoutineMaintain cultures between 2-6 x 100,000 cells/ml; 5% CO2; 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse T cell Hybridoma Quality Level 100, ImmunoDon B6DF mouse T cells Myeloma Azaguanine resistant BW5147 AKR mouse tumour cells Reactivity T cell hybridoma application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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FT
Кат. номер 90102525-1VL 90102525-DNA-5UG 90102525-RNA-5UG Cell Line OriginBull frog tongueCell Line DescriptionDerived from the tongue of a normal female bull frog. Incubation is optimal at 25°C but growth at a slower rate can continue even at 4°C where cells may be stored for prolonged periods.Culture MediumEMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Distilled H2O + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.02% EDTA at 10°C; 25°C. Capable of growth down to 4°C. Flask should not be gassed. Optimum pH is 8.0Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source frog mouth growth mode Adherent karyotype Modal no. 43 morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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FTC-133 Cell Line human 94060901-1VL
Cell Line OriginHuman follicular thyroid carcinoma, lymph node metastasisCell Line DescriptionFTC-133 was obtained from a lymph node metastasis of a follicular thyroid carcinoma not the primary tumour as originally reported from a 42-year-old male. The morphology differs from flat polygonal to spindle formed cells. They retained differentiated thyrocyte function and thyrocyte responsiveness to thyrotropin and local active growth factors. Thyroglobulin immunoreactivity could be shown in the cytoplasm and EGF receptor immunoreactivity could be visualised on the cell membranes. Complex chromosomal changes were detected as well as a p53 mutation. FTC-133 (Sigma catalogue number 94060901), FTC-236 (Sigma catalogue number 06030202) and FTC-238 (Sigma catalogue number 94060902) were all derived from the same individual, a 42 year old male with follicular thyroid cancer. This has been confirmed by short tandem repeat STR-PCR analysis at ECACC The Y chromosome could not be detected in these cell line by (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell lines are identical to the source provided by the depositor based on the STR-PCR analysis.ApplicationFTC-133 cell line has been used to study the function of human thyroid and development of thyroid cancer.DNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 9,10
THO1: 9.3
TPOX: 9
vWA: 15,18Culture MediumDMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:8 to 1:12 i.e. seeding at 1-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human thyroid (lymph node metastasis) Quality Level 100, description Human follicular thyroid carcinoma growth mode Adherent karyotype Complex chromosomal changes morphology Polymorphic products Expression of 5 - Deiodinase Type I receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) metastasis shipped in dry ice storage temp. 196°C
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FTC-236
Кат. номер 6030202-1VL 6030202-CDNA-20UL 6030202-RNA-5UG 6030202-DNA-5UG Cell Line OriginHuman Thyroid Cancer Neck Lymph Node MetastasisCell Line DescriptionDerived from a neck lymph node metastasis of a follicular thyroid cancer (FTC).This cell line is tumourigenic in Balb/c mice. FTC-133 (Sigma Catalogue number 94060901), FTC-236 (Sigma Catalogue number 06030202) and FTC-238 (Sigma Catalogue number 94060902) were all derived from the same individual, a 42 year old male with follicular thyroid cancer. This has been confirmed by short tandem repeat STR-PCR analysis at ECACC The Y chromosome could not be detected in these cell line by (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell lines are identical to the source provided by the depositor based on the STR-PCR analysis.Culture MediumDMEM and Ham′s F12 (1:1 mixture) + 10ug/ml Insulin + 0.01U/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human thyroid (lymph node metastasis) growth mode Adherent karyotype Not specified morphology Endothelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) metastasis shipped in dry ice storage temp. −196°C
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FTC-238
Кат. номер 94060902-1VL 94060902-CDNA-20UL 94060902-DNA-5UG 94060902-RNA-5UG Cell Line OriginHuman follicular thyroid carcinoma, lung metastasisCell Line DescriptionFTC-238 was established from a lung metastasis of a follicular thyroid carcinoma from a 42-year-old male. The cells are polymorphic showing flat polygonal to spindle-like morphologies. Complex chromosomal changes were detected as well as a p53 mutation. FTC-133 (Sigma Catalogue number 94060901), FTC-236 (Sigma Catalogue number 06030202) and FTC-238 (Sigma Catalogue number 94060902) were all derived from the same individual, a 42 year old male with follicular thyroid cancer. This has been confirmed by short tandem repeat STR-PCR analysis at ECACC The Y chromosome could not be detected in these cell line by (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell lines are identical to the source provided by the depositor based on the STR-PCR analysis.ApplicationFTC-238 has been used to study the function of human thyroid and development of thyroid cancer.DNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 9,10
THO1: 9.3
TPOX: 9
vWA: 15,18Culture MediumDMEM:Ham′s F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human thyroid (lung metastasis) growth mode Adherent karyotype Not specified morphology Polymorphic products Expression of 5′ - Deiodinase Type I receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) metastasis shipped in dry ice storage temp. −196°C
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FUEA human cell line
Кат. номер 94071413-CDNA-20UL 94071413-DNA-5UG 94071413-RNA-5UG 94071413-1VL General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileHLA DRB1: 8041Serological ProfileHLA DR: 1 8
HLA DQ: 5 7Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity African American gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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FW 11.10.3
Кат. номер 93070776-RNA-5UG 93070776-1VL 93070776-DNA-5UG Cell Line OriginMouse BALB/c x Mouse Sp2/0 myelomaCell Line DescriptionThe antibody is directed against the variant region of human CD44 encoded by exon 4. Staining binding must be performed at room temperature.ImmunogenFusion protein of human CD44ApplicationImmunohistology and FACSCulture MediumIDMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma Sp2/0 Reactivity Anti-CD44 v4, human application(s) cell culture | mammalian: suitable isotype IgG2a shipped in dry ice storage temp. −196°C
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FW 11.24.7.36
Кат. номер 93070775-1VL 93070775-DNA-5UG 93070775-RNA-5UG Cell Line OriginMouse BALB/c x Mouse Sp2/0 myelomaCell Line DescriptionThe antibody is directed against the variant region of human CD44 encoded by exon 9.ImmunogenFusion protein of human CD44ApplicationImmunohistology and FACSCulture MediumIDMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma Sp2/0 Reactivity Anti-CD44 v9, human application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. −196°C
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FW 11.9.2.2
Кат. номер 93070778-1VL 93070778-RNA-5UG 93070778-DNA-5UG Cell Line OriginMouse BALB/c x Mouse Sp2/0 myelomaCell Line DescriptionThe antibody is directed against the variant region of human CD44 encoded by exon 6. The antibody is suitable for immunohistology, but if used for FACS staining binding must be performed at room temperature.ImmunogenFusion protein of human CD44 v6ApplicationImmunohistology and FACSCulture MediumIDMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma Sp2/0 Reactivity Anti-CD44 v6, human application(s) cell culture | mammalian: suitable isotype IgG2a shipped in dry ice storage temp. −196°C
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FW 4.101
Кат. номер 93070777-DNA-5UG 93070777-RNA-5UG 93070777-1VL Cell Line OriginMouse Sp2/0 x Mouse BALB/c spleenCell Line DescriptionThe antibody is directed against 1 integrin CD29 of many species including human, cattle and sheep, and shows an identical pattern of reactivity as other anti 1 integrin antibodies e.g. TS2/16.ImmunogenSheep T cellsApplicationDetection of 1 integrin by FACS or immunohistologyCulture MediumIDMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Following resuscitation the serum concentration should be increased to 20% and the tissue culture flask incubated in an upright position for 4-7 days until the doubling rate increases.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma Sp2/0 Reactivity Anti-CD29 (β1 integrin) application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. −196°C
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FW14.14.18
Кат. номер 93100512-DNA-5UG 93100512-1VL 93100512-RNA-5UG Cell Line DescriptionThe antibody is directed against human α-6 integrin and will block the binding of other α-6 integrin antibodies such as EA-1. The antibody specifically stains α-6 transfected cells.Culture MediumIDMEM + 2mM glutamine + 1% Hypoxanthine, Aminopterin, Thymidine (HAT) + 10% Foetal Bovine Serum (FBS) (may benefit from IL-6 in medium)Subculture RoutineMaintain cultures between 3-5x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Unknown ImmunoDon Not specified Myeloma Not specified Reactivity Anti-α-6 Integrin, human application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. −196°C
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FW3-47
Кат. номер 91060559-DNA-5UG 91060559-RNA-5UG 91060559-1VL Cell Line OriginMouse Sp2/0 x Mouse BALB/c spleenCell Line DescriptionThe antibody detects the sheep CD29 (VLA 1 integrin) molecule expressed by virtually all sheep T and B cells, thymocytes and monocytes. It shows species cross-reactivity with cattle and goats.ImmunogenSheep T lymphocytesCulture MediumDMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 2-6x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma Sp2/0 Reactivity Anti-CD29, ovine application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. −196°C
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FW3.132.6
Кат. номер 93100514-RNA-5UG 93100514-DNA-5UG 93100514-1VL Cell Line DescriptionThe antibody is directed against a molecule expressed on most sheep leukocytes, platelets and endothelium. Its molecular weight could not be determined but may be equivalent to sheep CD3. The molecule is down-regulated in activated and memory T cells.ImmunogenSheep lymphocytesCulture MediumIDMEM + 2mM glutamine + 1% Hypoxanthine, Aminopterin, Thymidine (HAT) + 10% Foetal Bovine Serum (FBS) (may benefit from IL-6 in medium)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Unknown ImmunoDon Not specified Myeloma Not specified Reactivity Anti-Leukocyte Molecules, sheep application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. −196°C
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FW3.151
Кат. номер 93100515-1VL 93100515-DNA-5UG 93100515-RNA-5UG Cell Line DescriptionThe antibody is directed against a molecule similar to the 44kDa molecule recognised by FW1.136 which is expressed on all sheep leukocytes, red blood cells and platelets.ImmunogenSheep lymphocytesCulture MediumIDMEM + 2mM glutamine + 1% Hypoxanthine, Aminopterin, Thymidine (HAT) + 10% Foetal Bovine Serum (FBS) (may benefit from IL-6 in medium)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Unknown ImmunoDon Not specified Myeloma Not specified Reactivity Anti-Leukocytes, 44kDa antigen, sheep application(s) cell culture | mammalian: suitable isotype IgG2b shipped in dry ice storage temp. −196°C
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G
Кат. номер 94101453-1VL 94101453-DNA-5UG 94101453-RNA-5UG Cell Line OriginRat Dunning R-3327,prostate adenocarcinomaCell Line DescriptionDerived from a Dunning R-3327 rat with prostatic adenocarcinoma. G is one of a group of variants which developed by a single in vivo passage. It is androgen responsive, moderately fast growing and poorly differentiated. Using this system of rat prostatic adenocarcinomas as a model, it has been demonstrated that prostatic cancer cells can become unstable at any time resulting in the addition of cytogenetically and phenotypically novel clones of cancer cells to the cancer population.Culture MediumRPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat prostate growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors Androgen sensitive application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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G-292 Clone A141B1
Кат. номер 90110522-CDNA-20UL 90110522-DNA-5UG 90110522-1VL 90110522-RNA-5UG Cell Line OriginHuman Caucasian osteosarcomaCell Line DescriptionDerived from a primary osteosarcoma of a 9 year old Caucasian female. Cells exhibit the type B phenotype for G6PD and the karyotype is subtetraploid with chromosome markers.ApplicationTumourigenicity studiesCulture MediumMcCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/0.02% EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human bone growth mode Adherent karyotype Modal no. 57 morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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G-361
Кат. номер 88030401-1VL 88030401-RNA-5UG 88030401-CDNA-20UL 88030401-DNA-5UG Cell Line OriginHuman Caucasian malignant melanomaCell Line DescriptionEstablished from a malignant melanoma of a 31 year old male Caucasian. The cells produce melanin for up to 50 population doublings. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.ApplicationHormone studiesDNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 15
D5S818: 11
D7S820: 8,11
THO1: 7,9
TPOX: 8,10
vWA: 15,17Culture MediumMcCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells are slow in attaching.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human skin growth mode Adherent karyotype Triploid, modal n.o 69 morphology Epithelial products Melanin receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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G-401
Кат. номер 87042204-RNA-5UG 87042204-1VL 87042204-CDNA-20UL 87042204-DNA-5UG Cell Line OriginHuman Caucasian rhabdoid tumour (formerly classified as Wilm′s tumour)Cell Line DescriptionDerived from a tumour of a 3 month old male Caucasian. Highly transformed and grows in soft agar. Highly undifferentiated. G401 was originally described as a cell line derived from a Wilm?s tumour. Due to a change in the classification of such tumours, the cell line was examined by Garvin et al., 1993 and found to be more appropriately classified as derived from a rhabdoid tumour of the kidney.ApplicationTransformation and tumourigenicity studiesDNA ProfileSTR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 9,14
D16S539: 12
D5S818: 13
D7S820: 11,14
THO1: 8,9.3
TPOX: 8,11
vWA: 16Culture MediumMcCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Change medium every 4 days.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human kidney growth mode Adherent karyotype 2n = 46, diploid morphology Epithelial-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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G-402
Кат. номер 90112715-DNA-5UG 90112715-CDNA-20UL 90112715-1VL 90112715-RNA-5UG Cell Line OriginHuman Caucasian renal leiomyoblastomaCell Line DescriptionDerived from a tumour of a 9 month old female Caucasian. Highly transformed and grows in soft agar.ApplicationTransformation and tumourigenicity studiesDNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 9,12
D16S539: 11,13
D5S818: 11,12
D7S820: 9,10
THO1: 7,9
TPOX: 10
vWA: 13,17Culture MediumMcCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Change medium every 4 days.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human kidney growth mode Adherent karyotype Modal no. 47 morphology Epithelial-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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G-7
Кат. номер 90110520-DNA-5UG 90110520-1VL 90110520-RNA-5UG Cell Line OriginMouse Swiss-Webster myoblastCell Line DescriptionDerived from a muscle tissue culture of a foetal Swiss-Webster mouse. Tumorigenic and only slightly sensitive to acetylcholine. When confluent cultures form short multinucleated myotubes.ApplicationTumourigenicity studiesCulture MediumDMEM + 2mM Glutamine + 10% Horse Serum (HS) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin in Ca2+ + Mg2+-free PBS; 5% CO2; 37°C. Myotubes are formed at confluency, this is assisted by reducing both sera to 4%.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse muscle growth mode Adherent karyotype Not specified morphology Myoblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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G-8
Кат. номер 89050906-DNA-5UG 89050906-RNA-5UG 89050906-1VL Cell Line OriginMouse Swiss-Webster myoblastCell Line DescriptionIsolated from a myogenic line derived from hind limb muscle of a foetal Swiss-Webster mouse. Non-tumorigenic and highly sensitive to acetylcholine. Confluent cultures form multinucleated myotubes.Culture MediumDMEM + 2mM Glutamine + 10% Horse Serum (HS) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Myotubes are formed at confluency, this is assisted by reducing both sera to 4%.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse muscle growth mode Adherent karyotype Not specified morphology Myoblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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GA4
Кат. номер 85062502-DNA-5UG 85062502-RNA-5UG 85062502-1VL Cell Line OriginMouse NS1 x Sheep lymph nodeCell Line DescriptionThis HAT sensitive line can be used as a fusion partner in the production of heterohybridomas.ImmunogenBovine red blood cellsApplicationFusion partnerCulture MediumRPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS)Subculture RoutineMaintain cultures between 2-6x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Sheep Heterohybridoma ImmunoDon Sheep lymph node Myeloma NS1 Reactivity Non Ig secreting application(s) cell culture | mammalian: suitable isotype None shipped in dry ice storage temp. −196°C
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GAD-1
Кат. номер 92020601-DNA-5UG 92020601-1VL 92020601-RNA-5UG Cell Line OriginMouse Sp2/0 x Mouse BALB/c spleenCell Line DescriptionThe antibody is directed against glutamic acid decarboxylase from chickens, mice, rats, rabbits, cats and macaque monkeys.ImmunogenGlutamic acid decarboxylaseCulture MediumDMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 2-6x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma Sp2/0 Reactivity Anti-Glutamic acid decarboxylase application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. −196°C
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GEE018 human cell line
Кат. номер 94050329-DNA-5UG 94050329-1VL 94050329-RNA-5UG 94050329-CDNA-20UL General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 0203 2901
Cw: 0401 15
B: 0705 1519Serological ProfileHLA A: 2 29
HLA C: 6
HLA B: 7 76
Bw4/bw6: 6Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Chinese gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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GEEP
Кат. номер 92062401-1VL 92062401-DNA-5UG 92062401-RNA-5UG Cell Line OriginGoat x Sheep hybrid, skin.Cell Line DescriptionSkin fibroblast derived from a 6 year old female goat x sheep interspecies hybrid (Capra Lircus x Ovis Aries).Culture MediumEMEM α (nucleotide enriched) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source ovine hybrid growth mode Adherent karyotype 2n = 57 morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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GFP-KSC4 Keratinocyte stem cell line
Кат. номер 11081701-1VL 11081701-DNA-5UG Cell Line OriginMurine keratinocyte stem cell line, genetically modified,Cell Line DescriptionKeratinocytes were isolated from neonatal homozygous C57BL/6 Tg(CAG EGFP)1Osb/J mouse skin. The cell line was established by growing cells for the first 8 passages in co-culture with 3T3 J2 fibroblast feeder cells. From passage 9 onwards cells were grown without feeder cells. This line can be used for the generation of in vitro epidermal skin equivalents to be used as screening tools to look at the activity of NCEs and/or NBEs of dermatological interest, and/or to screen for such NCE and/or NBE in high throughput systems. It may also be of use to non pharmaceutical healthcare and/or cosmetic companies involved in the personal vitality and/or beauty product development segments. The spontaneously immortalized keratinocytes can be differentiated by the addition of calcium to the complete FAD medium at a final concentration of 1.2 mM (high calcium medium). Confluent cultures stratify when grown in high calcium medium.DNA ProfileNot specifiedCulture MediumThe cell line is grown in low calcium medium, DMEM/Ham′s F12 with FBS / FCS, (F2442) and several supplements at 32 °C, 5% CO2. The supplements are: 0.18 mM adenine, 0.5 µg/ml hydrocortisone, 5 µg/ml insulin,10 -10 M cholera toxin, 10 ng/ml EGF, 2 mM L-Glutamine, (G7513), 1 mM pyruvate.Subculture RoutineWash monolayer with PBS (without Ca2+ and Mg2+), incubate with EDTA solution for 5 min at room temperature and finally incubate for 8-12 min with 0.05% trypsin/0.02% EDTA at 37 °C until they come off easily. Add complete FAD medium and resuspend the cells by pipetting up and down 3-5 times. After centrifugation for 5 min at 220 g, resuspend the pellet in complete FAD medium, and seed the cells onto collagen I-coated dishes. Seed new cultures at 1.5-3 x 104 cells/cm2.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source mouse skin growth mode Adherent karyotype Not specified morphology Polygonal receptors Not specified
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GH1
Кат. номер 89072610-RNA-5UG 89072610-DNA-5UG 89072610-1VL Cell Line OriginRat Wistar-Furth pituitary tumourCell Line DescriptionDerived from pituitary gland of Wistar-Furth rats. The cells secrete growth hormone at a rate of 1μg/ml cell protein/day. Re-inoculation of cloned cells back to Wistar-Furth rats results in a marked increase in body weight and stimulation of linear growth in both intact and hypophysectomized animals independent of rat tumour size.ApplicationHormone studiesCulture MediumHam′s F10 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. These cells only semi-attach and may return to suspension during transit. If this has occurred on receipt of the cells pellet cells bOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat brain growth mode Semi-adherent karyotype Hypodiploid, modal no. 40. morphology Epithelial products Rat growth hormone, lactogenic hormones. receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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GH3
Кат. номер 87012603-DNA-5UG 87012603-1VL 87012603-RNA-5UG Cell Line OriginRat Wistar-Furth pituitary tumourCell Line DescriptionDerived from a 7 month female Wistar-Furth rat. GH3 cells produce growth hormone faster than the GH1 cell line and also produce prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.ApplicationHormone studiesCulture MediumHam′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Upon thawing, the time taken before the first subculture can be performed may be extended (10-15 days). To aid attatchment, flaskOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat brain growth mode Adherent karyotype Hyperdiploid, modal no. 60 morphology Epithelial products Growth hormone, prolactin receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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GIRARDI HEART (HeLa derivative)
Кат. номер 93120822-DNA-5UG 93120822-1VL 93120822-CDNA-20UL 93120822-RNA-5UG Cell Line OriginHuman cervix carcinomaCell Line DescriptionOriginally derived from a biopsy specimen of the right auricular appendage of an adult human heart. The cells support the growth of polio viruses 1 and 3, adenovirus 3 and VSV (Indiana strain). This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.ApplicationVirus studiesCulture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human cervix growth mode Adherent karyotype 2n = 45 morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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GL-1
Кат. номер 89072612-1VL 89072612-DNA-5UG 89072612-RNA-5UG Cell Line OriginLizard Gekko lungCell Line DescriptionFrom adult male Tokay Gekko (indigenous to Southeast Asia). Observed to undergo spontaneous chromosomal alterations from a normal diploid number of 38 to a bimodal distribution of chromosome number 38-40. Susceptible to a number of mammalian viruses at 36°C.ApplicationChromosome and virus studiesCulture MediumEMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 30°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source lizard lung growth mode Adherent karyotype Modal no. 37 and 38 morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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GO85 human cell line
Кат. номер 94050313-CDNA-20UL 94050313-DNA-5UG 94050313-RNA-5UG 94050313-1VL General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 0101 2901
Cw: 12022 1502
B: 4006 5201
HLA DRB1: 15 14
HLA DQB1: 05 06Serological ProfileHLA A: 1 29
HLA B: 52 61
HLA DR: 15 6
HLA DR52: 52
HLA DQ: 1Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Spanish Roma gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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GP2d
Кат. номер 95090714-1VL 95090714-RNA-5UG 95090714-CDNA-20UL Cell Line OriginHuman Caucasian colon adenocarcinomaCell Line DescriptionGP2d has been established from the same adenocarcinoma as GP5d (Sigma Catalogue number. 95090715). This has been confirmed by STR profiling at ECACC. The cells were derived from a local recurrence of Duke′s grade B, poorly differentiated carcinoma of the colon from a 71 year old female at surgical resection. The patient received no preoperative chemo- or radiotherapy and had a strong family history of colon cancer with two first degree relatives dying of the disease below the age of 55 years. Both clones possess genetic changes consistent with the pattern of tumour progression in colon cancer but are morphologically distinct. An interstitial deletion on chromosome 5 (region 14q to 22q) and an inverted duplication of bands 10q11 and 10q21 has been reported. Both cell lines contain a normal copy of the ki-ras gene and a copy with a transition in codon 12. GP2d cells show growth response to EGF, TGF α or insulin with an increase in cell numbers. Amphiregulin mRNA is abundant in GP2d but almost undetectable in GP5d.ApplicationGP2d has been used to extract genomic DNA for digital PCR (dPCR). It has also been used to extract DNA from the cell with PIK3CA mutation c.3140A>T.DNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 9,12
D13S317: 8,12
D16S539: 11,13
D5S818: 11,12
D7S820: 9,11
THO1: 7,9.3
TPOX: 8,11
vWA: 17,18Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 5x10,000 to 1x100,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human colon growth mode Adherent karyotype Diploid morphology Epithelial products Not specified receptors Epidermal growth factor (EGF) application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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GP5d
Кат. номер 95090715-CDNA-20UL 95090715-1VL 95090715-RNA-5UG Cell Line OriginHuman Caucasian colon adenocarcinomaCell Line DescriptionGP5d has been established from the same adenocarcinoma as GP2d (Sigma Catalogue number. 95090714). This has been confirmed by STR profiling at ECACC. The cells were derived from a local recurrence of Duke′s grade B, poorly differentiated carcinoma of the colon from a 71 year old female at surgical resection. The patient received no preoperative chemo- or radio therapy and had a strong family history of colon cancer with two first degree relatives dying of the disease below the age of 55 years. Both clones possess changes consistent with the usual pattern of tumour progression in colon cancer but are morphologically distinct. An interstitial deletion on chromosome 5 (region 14q to 22q) and an inverted duplication of bands 10q11 and 10q21 has been reported. Both cell lines contain a normal copy of the ki-ras gene and a copy with a transition in codon 12. GP5d cells express relatively more TGF α mRNA than GP2d cells. GP5d cells undergo a marked epithelial-mesemchymal transition when treated with EGF.ApplicationColon tumour assays, influence of growth factors and adhesion molecules on colonic tumour cellsCulture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 5x10,000 to 1x100,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human colon growth mode Adherent karyotype Diploid morphology Epithelial products Not specified receptors Epidermal growth factor (EGF) application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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GPNT
Кат. номер 9061601-DNA-5UG 9061601-RNA-5UG 9061601-1VL Cell Line OriginAn immortalised Lewis rat brain vascular endothelial cell lineCell Line DescriptionGPNT was derived from the immortalised rat brain endothelial cell line GP8 by lipofectin-mediated transfection with a vector containing the puromycin resistance gene. GPNT exhibits a non-transformed phenotype.DNA ProfileNot specifiedCulture MediumHams F10 + 10% Heat-inactivated FCS + 2 mM Glutamine + 2 ng/ml basic fibroblast growth factor + 80 μg/ml heparin. All plasticware is pre-coated for several hours with collagen (Type I from calf skin) at 10μg/cm2 from a 0.01% solution in acetic acid. After removal the coated plasticware is exposed to a mMonia vapour for 5 min. Plasticware must be rinsed thoroughly with PBS prior to use.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approx 10hrs.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source rat brain growth mode Adherent karyotype Not specified morphology Fusiform products GPNT cells express tight junction proteins receptors P-glycoprotein, PECAM-1, ICAM-1, VCAM-1 Glut-1, transferrin receptor, MHC class I application(s) cell culture | mammalian: suitable shipped in dry ice
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GRC-138 human cell line
Кат. номер 94071442-RNA-5UG 94071442-1VL 94071442-CDNA-20UL 94071442-DNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 0201 0211
Cw: 304
B: 1504 4003
HLA DRB1: 16 1413
HLA DQB1: 03Serological ProfileHLA A: 2 2
HLA C: 9
HLA B: 62 60
Bw4/bw6: 6
HLA DR: 16 14
HLA DR52: 52
HLA DQ: 7Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Native South American, Guarani gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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GRC-187 human cell line
Кат. номер 94071443-CDNA-20UL 94071443-DNA-5UG 94071443-1VL 94071443-RNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 68012 31012
Cw: 0303 0304
B: 3511 1504
HLA DRB1: 1413 08
HLA DQB1: 03 04Serological ProfileHLA A: 68 31
HLA C: 9
HLA B: 35 62
Bw4/bw6: 6
HLA DR: 14 8
HLA DR52: 52
HLA DQ: 7 4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Native South American, Guarani gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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GRC-212 human cell line
Кат. номер 94071444-1VL 94071444-DNA-5UG 94071444-CDNA-20UL 94071444-RNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 0211 68012
Cw: 0401 0304
B: 3505 4004
HLA DRB1: 04 09
HLA DQB1: 03 03Serological ProfileHLA A: 2 68
HLA C: 4 9
HLA B: 35 40v
Bw4/bw6: 6
HLA DR: 4 9
HLA DR53: 53
HLA DQ: 3 3Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Native South American, Guarani gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
GRE, G human cell line
Кат. номер 94082261-CDNA-20UL 94082261-1VL 94082261-RNA-5UG 94082261-DNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileHLA DRB1: 0401 1501
HLA DRB4: 101
HLA DRB5: 101
HLA DQAP: 1.2 3.1
HLA DQA1: 0102 03
HLA DQB1: 0301 0602
HLA DPB1: 0401 0402Serological ProfileHLA A: 2 11
HLA A ief: 2.2 11.2
HLA B: 7 27
HLA B ief: 7.2 27.4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Caucasian gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
GRL101 (KC7)
Кат. номер 94071259-RNA-5UG 94071259-1VL 94071259-DNA-5UG Cell Line OriginHamster Chinese ovary transfected with Lymnaea receptor GRL101Cell Line DescriptionCell line containing the receptor protein GRL101. The cDNA from the CNS of the mollusc Lymnaea stagnalis encoding a putative receptor has been isolated and analysed. The first repeat of DNA displays a high sequence similarity to the extracellular binding domains of the low density protein receptor, which binds and internalises cholesterol containing apolipoproteins. The MRNI receptor is predominantly expressed in a small number of neurons within the CNS and to a lesser extent in the heart.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2, 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source hamster ovary growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors GRL101 - putative Lymnaea receptor application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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GRL101 (MIX)
Кат. номер 94071258-DNA-5UG 94071258-RNA-5UG 94071258-1VL Cell Line OriginHamster Chinese ovary transfected with Lymnaea receptor GRL101Cell Line DescriptionCell line containing the receptor protein GRL101. The cDNA from the CNS of the mollusc Lymnaea stagnalis encoding a putative receptor has been isolated and analysed. The first repeat of DNA displays a high sequence similarity to the extracellular binding of the low density protein receptor, which binds and internalises cholesterol containing apolipoproteins. The MRNI receptor is predominantly expressed in a small number of neurons within the CNS and to a lesser extent in the heart. Contains a mixed culture of approximately 20 transfected clones.ApplicationStudies in the newly characterised members of a putative class of G protein - linked receptors that directly transduce signals carried by large extracellular (lipo)protein (complexes) into neural events.Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2 - 4 x 10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2, 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source hamster ovary growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors GRL101 - putative Lymnaea receptor application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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Grunt Fin (GF)
Кат. номер 88010601-DNA-5UG 88010601-1VL 88010601-RNA-5UG Cell Line OriginFish Grunt fin tissue blue stripedCell Line DescriptionDerived from fin tissue of the adult salt water blue striped Grunt. Does not support the replication of influenza A, herpes simplex, adeno, polio, dengue and eastern equine encephalitis viruses.Culture MediumEMEM (HBSS) (Sigma cat no. M5775) + 10mM Hepes (pH 7.3) + 2mM Glutamine + 0.23% (w/v) NaHCO3 + 20% FBS (Heat Inactivated)+ 58mM NaClSubculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 20°C. After resuscitation split cells 1:2 to 1:4 and culture for at least one week with 1-2 media changes before shipping. Plating efficiency can be very low aOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source fish fin growth mode Adherent karyotype Modal no. 47 morphology Fibroblast-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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GS-109-V-20
Кат. номер 90110505-CDNA-20UL 90110505-1VL 90110505-DNA-5UG 90110505-RNA-5UG Cell Line OriginHuman Caucasian skin fibroblast Gardner′s syndromeCell Line DescriptionDerived from skin from the upper arm of an 18 year old Caucasian male with Gardner′s syndrome is an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. Cells exist in an initiated state rather than a normal or transformed state. Normal diploid karyotype.ApplicationTumourigenicity studiesCulture MediumEMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. Monolayer will form in a 25cm2 flask in 1 week.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human skin growth mode Adherent karyotype 2n = 46 morphology Fibroblast-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену
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