Cell Lines & Specialty Cell Culture
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CHOZN® CHO K1 Host Cell Line CHOK1-1VL
General descriptionA subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine γ serialdehyde. They undergo morphological changes in response to cholera toxin.
CHO-K1 cells derived from ECACC CHO K1 and adapted to suspension and serum-free, chemically defined media. Cells are cGMP banked in chemically defined, animal component-free EX-CELL® CD CHO Fusion medium.Cell Line OriginHamster Chinese ovary. The parental CHO-K1 cell line was originated by Puck in 1957.Physical formCHOZN CHO K1 cells are provided to customers in vials containing 1 mL at 107 cells/mL. Cells are banked in EX-CELL CD Fusion medium containing 4mM L glutamine and 7% DMSO.Legal InformationThe CHOZN CHO K1 cell line is sold for research use only. For use of this cell line in a commercial process, a commercial license must be taken.
CHOZN is a registered trademark of Sigma-Aldrich Co. LLC
EX-CELL is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 description CHOK1 cell line derived from ECACC CHO K1 shipped in dry ice storage temp. 196°C
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CHOZN® DHFR-/- ZFN-Modified CHO Cell Line
NACRES: NA.75
Кат. номер CHODHFR-1VL-PW CHODHFR-1VL General descriptionCHOZN cell lines were created utilizing Sigma′s proprietary CompoZr Zinc Finger Nuclease (ZFN) technology to create genetic modifications in parental CHO-K1 cells. Cells are banked in chemically defined, animal component free EX-CELL CD CHO Fusion mediaCell Line OriginCHOZN cell lines are all subclones of the parental CHO-K1 cell line originated by Puck in 1957.Physical formCHOZN cell lines are provided to customers in vials containing >7.5e6 cells/mLLegal InformationAll CHOZN cell lines are sold under license from Sigma Aldrich. A copy of the research license is provided with purchase of the CHOZN cell lines.
CHOZN is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 description CHOZN® DHFR-/- ZFN-modified CHO cell line, dihydrofolate reductase knockout cell line shipped in dry ice storage temp. 196°C
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CHOZN® GS-/- ZFN-modified CHO cell line CHOGS-1VL
NACRES: NA.75 General descriptionZFNs are a class of engineered DNA-binding proteins and causes a double-strand break. This double-strand break is necessary for site-specific mutagenesis mediated DNA repair. It facilitates targeted genome editing by binding to a user-specified locus. Glutamine synthetase (GS) is one of the most commonly used selectable markers in the biopharmaceutical industry. Glutamine is an essential amino acid for cellular growth. The GS enzyme is responsible for the conversion of glutamate into glutamine. Without this enzymatic activity, cells can no longer synthesize glutamine endogenously and if glutamine is not supplemented in the culture media, the cells will die.
CHOZN cell lines were created utilizing Sigma′s proprietary CompoZr Zinc Finger Nuclease (ZFN) technology to create genetic modifications in parental CHO-K1 cells. Cells are banked in chemically defined, animal component free EX-CELL CD CHO Fusion mediaCell Line OriginCHOZN cell lines are all subclones of the parental CHO-K1 cell line originated by Puck in 1957.PackagingThis product is shipped in liquid nitrogen dry vapor shippers.
Liquid nitrogen dry vapor shipper fee (up to a maximum of $125) and freight not included in price of product.
Liquid nitrogen dry vapor shipper is not disposable and should be returned after receipt. A $450 liquid nitrogen dry vapor shipper fee will be charged if not returned within 1 week of delivery.
Learn more about liquid nitrogen dry vapor shipperPhysical formCHOZN cell lines are provided to customers in vials containing >7.5e6 cells/mLLegal InformationAll CHOZN cell lines are sold under license from Sigma Aldrich. A copy of the research license is provided with purchase of the CHOZN cell lines.
CHOZN is a registered trademark of Sigma-Aldrich Co. LLCПараметрыdescription CHOZN® GS-/- ZFN-modified CHO cell line, glutamine synthetase knockout cell line solubility DMSO: 5 mg/mL, clear shipped in liquid nitrogen storage temp. 196°C
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CompoZr® Knockout ZFN Kit- CHO DHFR
NACRES: NA.75
Кат. номер ZFNDHFR-50UG ZFNDHFR-50UG-PW General descriptionCompoZr Knockout ZFNs create targeted double strand breaks at a gene specific target locus. Through the cellular process of Non-Homologous End Joining, this double strand break can result in modification of the DNA sequence and therefor create a functional knockout of the targeted gene. To provide the best chance of creating a functional gene knockout, CompoZr Knockout ZFNs are targeted to the first 2/3 of the coding region for the gene of interest.Physical formCompoZr Knockout ZFNs are provided as two plasmids encoding for the gene specific zinc finger nuclease.Legal InformationAll Zinc Finger Nucleases are sold under license from Sangamo Biosciences. CompoZr is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 product line CompoZr® shipped in dry ice storage temp. 20°C
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CompoZr® Knockout ZFN Kit- CHO GS
NACRES: NA.75
Кат. номер ZFNGS-1KT ZFNGS-1KT-PW General descriptionCompoZr Knockout ZFNs create targeted double strand breaks at a gene specific target locus. Through the cellular process of Non-Homologous End Joining, this double strand break can result in modification of the DNA sequence and therefor create a functional knockout of the targeted gene. To provide the best chance of creating a functional gene knockout, CompoZr Knockout ZFNs are targeted to the first 2/3 of the coding region for the gene of interest.Physical formCompoZr Knockout ZFNs are provided as two plasmids encoding for the gene specific zinc finger nuclease.Legal InformationAll Zinc Finger Nucleases are sold under license from Sangamo Biosciences. CompoZr is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 product line CompoZr® shipped in dry ice storage temp. 20°C
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CompoZr® Knockout ZFN Kit- CHO SLC35A1 ZFNSLC35A1-1KT
General descriptionCompoZr Knockout ZFNs create targeted double strand breaks at a gene specific target locus. Through the cellular process of Non-Homologous End Joining, this double strand break can result in modification of the DNA sequence and therefore create a functional knockout of the targeted gene. To provide the best chance of creating a functional gene knockout, CompoZr Knockout ZFNs are targeted to the first 2/3 of the coding region for the gene of interest.Physical formCompoZr Knockout ZFNs are provided as 10 aliquots of mRNA encoding for the ZFNs along with plasmid DNA for both ZFN pairs. Also included in the kit are custom-designed primers, that allow for determination of the rate of mutation and for screening of individual clones that have the desired mutational event, and positive control DNA that has been modified using the CompoZr Knockout ZFN.Legal InformationAll Zinc Finger Nucleases are sold under license from Sangamo Biosciences. CompoZr is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 description Solute Carrier Family 35 (CMP-Sialic Acid Transporter), Member A1 product line CompoZr® shipped in dry ice storage temp. 70°C Gene Information hamster ... Slc35a1(100689322)
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#W7079 REM human cell line
Кат. номер 94022552-1VL 94022552-CDNA-20UL 94022552-DNA-5UG 94022552-RNA-5UG General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 0201 0206
Cw: 03 07
B: 6701 40011Serological ProfileHLA A: 2
HLA C: 10
HLA B: 60 67
Bw4/bw6: 6Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Caucasian gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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07-4D
Кат. номер 91121239-DNA-5UG 91121239-RNA-5UG 91121239-1VL Cell Line OriginMouse NS1 x Mouse BALB/c spleenCell Line DescriptionThe antibody is directed against desmocollins (desmosomal glycoproteins 2 and 3) from bovine nasal epidermis.ImmunogenBovine desmocollins (desmosomal glycoproteins 2 and 3) from nasal epidermisCulture MediumDMEM + 2mM glutamine + 20% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma NS1 Reactivity Anti-Desmocollins, bovine application(s) cell culture | mammalian: suitable isotype IgG shipped in dry ice storage temp. −196°C
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1-7HB2
Кат. номер 10081201-RNA-5UG 10081201-CDNA-20UL 10081201-1VL 10081201-DNA-5UG Cell Line OriginBreast cancer, morphology, adhesion propertiesCell Line Description1-7HB2 is a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7 identified by screening for branching morphology. The MTSV1-7 is a clonal non-tumourigenic cell line developed by immortalizing luminal epithelial cells cultured from milk using the SV40 T antigen. (See also MTSV1-7 CE1 Sigma catalog number 10081202.). Used for in vitro study of branching morphogenesis of human mammary epithelial cells and the role of integrins in mammary morphogenesis.DNA ProfileSTR-PCR Data:
Amelogenin: X
CSF1PO: 12,13
D13S317: 12
D16S539: 8,13
D5S818: 10,13
D7S820: 8,10
THO1: 7,9
TPOX: 11
vWA: 14,17Culture MediumDMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + Hydrocortisone (5 μg/ml) + Bovine Insulin (10 μg/ml).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human breast growth mode Adherent karyotype Not specified morphology Cuboidal products Formation of branching structures in the presence of fibroblast cultures or fibroblast-conditioned medium. Formation of spheroids in collagen type I gels. receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice
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1.2B4
Кат. номер 10070103-CDNA-20UL 10070103-RNA-5UG 10070103-DNA-5UG 10070103-1VL Cell Line Description1.2B4 is a hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with HuP-T3, a human pancreatic carcinoma cell line (Sigma catalog number 93121055). 1.2B4 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology. 1.2B4 cells provide a method of producing pure insulin secreting cells. This is an alternative to the use of primary tissue in cell transplantation therapies for type 1 diabetes.DNA ProfileSTR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10
D13S317: 9,11
D16S539: 10,13
D5S818: 10,14
D7S820: 12
THO1: 9
TPOX: 8,12
vWA: 18Culture MediumRPMI-1640 + 2 mM Glutamine + 10% FCS / FBSSubculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:6 , i.e., seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approx 20hrs. At confluence 105 cells/cm2 can be expected.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Legal InformationFlatt P & McClenaghan N (2004) Insulin producing cell-line US patent number 20040005702 (A1). Also WO0210346 (A2).DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human pancreas (islets) growth mode Adherent karyotype Modal chromosome number 67-71 morphology Epitheloid products 1.2B4 cells have been shown to express insulin, glucokinase and IAPP by immunocytochemistry. These cells express the GLUT1 glucose transporter. receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice
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1.4E7
Кат. номер 10070102-RNA-5UG 10070102-DNA-5UG 10070102-CDNA-20UL 10070102-1VL Cell Line Description1.4E7 is a hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with PANC-1, a human pancreatic ductal carcinoma cell line (ECACC catalogue number 87092802). 1.4E7 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology. 1.4E7 cells provide a method of producing pure insulin secreting cells when stimulated. This is an alternative to the use of primary tissue in cell transplantation therapies for type 1 diabetes. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.DNA ProfileSTR-PCR Data:
Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11
D5S818: 11,13
D7S820: 8
THO1: 7,8
TPOX: 8,11
vWA: 15Culture MediumRPMI-1640 + 2 mM Glutamine + 10% FCS / FBSSubculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:6 , i.e., seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approx 26hrs. At confluence 105 cells/cm2 can be expected.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Legal InformationFlatt P & McClenaghan N (2004) Insulin producing cell-line US patent number 20040005702 (A1). Also WO0210346 (A2).DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human pancreas (islets) growth mode Not specified karyotype Modal chromosome number 67-72 morphology Epitheloid products 1.4E7 cells have been shown to express insulin, glucokinase and IAPP by immunocytochemistry. These cells express the GLUT1 glucose transporter. receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice
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10(AAT) Clone A
Кат. номер 2043015-DNA-5UG 2043015-1VL 2043015-RNA-5UG Cell Line OriginMouse Sp2/0 x Mouse BALB/c spleenCell Line DescriptionThe antibody is directed against α1-antitrypsin (also known as α1-AT or AAT).ImmunogenVarious peptides between 9 and 11 amino acids attached to PPD via a terminal cysteine residueApplicationELISA, lateral flow tests, western blot analysisCulture MediumEMEM + 2mM glutamine + 10 - 15% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Split ratio: 1:2Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma Sp2/0 Reactivity Anti-α1-Antitrypsin (also known as α1-AT or AAT) application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. −196°C
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104C1
Кат. номер 90110524-RNA-5UG 90110524-DNA-5UG 90110524-1VL Cell Line OriginGuinea pig foetal cells transformedCell Line DescriptionDerived from a female foetal carcass of a 2/N guinea pig. The cells were treated with benzopyrene for 7 days and a clone selected at the 20th passage. The cells are tumourigenic in guinea pigs.Culture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5 x10,000 cells/cm3 using 0.25% trypsin; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source guinea pig embryo growth mode Adherent karyotype Diploid morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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108CC15
Кат. номер 8062516-1VL 8062516-DNA-5UG 8062516-RNA-5UG Cell Line OriginMouse neuroblastoma x Rat glioma hybridCell Line DescriptionThe cell line108CC15 is formed by the fusion of mouse neuroblastoma N18TG2 and rat glioma C6-BU-1using inactivated Sendai virus. This cell line was renamed NG108-15 by Klee and Nirenberg. Sigma has two catalogue entries for the 108CC15 cell line which differ in that they were deposited by different groups: 108CC15 from the original group that derived the cell line (Sigma Catalogue number 08062516) and NG108-15 (Sigma Catalogue number 88112302). Several other cell lines in the Sigma catalogue have been derived from 108CC15. This is a cholinergic hybrid cell line.ApplicationUsed as a neuronal ModelCulture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured inbetween in HT medium for 3-4 daysSubculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma nerve growth mode Adherent karyotype Modal No. varied with passage see reference morphology Neuronal products Long processes after treatment with dibutyryl cAMP, dense core vesicles, excitable membranes, neurotransmitter enzymes, synthesis of neurohormones and uptake of catecholamines. receptors Receptors for neurohormones: delta opioid, PGE1 vasoactive intestinal polypeptide, adenosine, somatostatin and acetylcholine. application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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108CC5
Кат. номер 8062521-1VL 8062521-DNA-5UG Cell Line OriginMouse neuroblastoma x Rat glioma hybridCell Line DescriptionThe cell line108CC5 is formed by the fusion of mouse neuroblastoma N18TG2 and rat glioma C6-BU-1using inactivated Sendai virus. This cell line was renamed NG108-5 by Klee and Nirenberg . This is a cholinergic hybrid cell line.ApplicationUsed as a neuronal ModelCulture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured inbetween in HT medium for 3-4 daysSubculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma nerve growth mode Adherent karyotype Modal No. varied with passage see reference morphology Neuronal products Long processes after treatment with dibutyryl cAMP, dense core vesicles, excitable membranes, neurotransmitter enzymes, synthesis of neurohormones and uptake of catecholamines. receptors Receptors for neurohormones: delta opioid, PGE 1 vasoactive intestinal polypeptide, adenosine, somatostatin and acetylcholine. application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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108CC5-BU
Кат. номер 8062566-1VL 8062566-DNA-5UG 8062566-RNA-5UG Cell Line OriginMouse neuroblastoma x Rat glioma hybridCell Line DescriptionA bromodeoxyuridine resistant mutant of 108CC5 (Sigma Catalogue number 08062521), a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybridApplicationUsed in the generation of cell hybrids.Culture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridine.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required. Immediately after resuscitation a pre-centrifugation step to remove cryoprotectant (DMSO) is recommended i.e. pellet the cells by centrifugation at 100 x g for 5 minutes and resuspend the cell pellet in fresh medium using the appropriate volume to achieve the correct seeding density.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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108CC5-BU-5
Кат. номер 8062567-RNA-5UG 8062567-DNA-5UG 8062567-1VL Cell Line OriginMouse neuroblastoma x Rat glioma hybridCell Line DescriptionA bromodeoxyuridine resistant mutant of 108CC5 (Sigma Catalogue number 08062521), a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybridApplicationUsed in the generation of cell hybrids.Culture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridineSubculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required. Immediately after resuscitation a pre-centrifugation step to remove cryoprotectant (DMSO) is recommended i.e. pellet the cells by centrifugation at 100 x g for 5 minutes and resuspend the cell pellet in fresh medium using the appropriate volume to achieve the correct seeding density.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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108CC5-BU-8
Кат. номер 8062569-RNA-5UG 8062569-DNA-5UG 8062569-1VL Cell Line OriginMouse neuroblastoma x Rat glioma hybridCell Line DescriptionA bromodeoxyuridine resistant mutant of 108CC5 (Sigma Catalogue number 08062521), a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybridApplicationUsed in the generation of cell hybrids.Culture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridine.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required. Immediately after resuscitation a pre-centrifugation step to remove cryoprotectant (DMSO) is recommended i.e. pellet the cells by centrifugation at 100 x g for 5 minutes and resuspend the cell pellet in fresh medium using the appropriate volume to achieve the correct seeding density.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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108CC5-TG-1
Кат. номер 8062543-DNA-5UG 8062543-1VL 8062543-RNA-5UG Cell Line OriginMouse neuroblastoma x Rat glioma hybridCell Line DescriptionA 6-thioguanine resistant mutant of 108CC5 (Sigma Catalogue number 08062521), a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybridApplicationUsed as a neuronal ModelCulture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine. The cells can also be grown in the absence of 6-thioguanine.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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108CC5-TG-2
Кат. номер 8062544-RNA-5UG 8062544-1VL 8062544-DNA-5UG Cell Line OriginMouse neuroblastoma x Rat glioma hybridCell Line DescriptionA 6-thioguanine resistant mutant of 108CC5 (Sigma Catalogue number 08062521), a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybridApplicationUsed as a neuronal ModelCulture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine. The cells can also be grown in the absence of 6-thioguanine.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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108CC5-TG-3
Кат. номер 8062545-1VL 8062545-DNA-5UG 8062545-RNA-5UG Cell Line OriginMouse neuroblastoma x Rat glioma hybridCell Line DescriptionA 6-thioguanine resistant mutant of 108CC5 (Sigma Catalogue number 08062521), a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybridApplicationUsed as a neuronal ModelCulture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine. The cells can also be grown in the absence of 6-thioguanine.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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108CC5-TG-4
Кат. номер 8062546-RNA-5UG 8062546-1VL 8062546-DNA-5UG Cell Line OriginMouse neuroblastoma x Rat glioma hybridCell Line DescriptionA 6-thioguanine resistant mutant of 108CC5 (Sigma Catalogue number 08062521), a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybridApplicationUsed as a neuronal ModelCulture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine. The cells can also be grown in the absence of 6-thioguanine.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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108CC5T-BU-1
Кат. номер 8062570-DNA-5UG 8062570-1VL 8062570-RNA-5UG Cell Line OriginMouse neuroblastoma x Rat glioma hybridCell Line DescriptionA bromodeoxyuridine resistant mutant of 108CC5 (Sigma Catalogue number 08062521), a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybrid.ApplicationUsed in the generation of cell hybrids.Culture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridine.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required. Immediately after resuscitation a pre-centrifugation step to remove cryoprotectant (DMSO) is recommended i.e. pellet the cells by centrifugation at 100 x g for 5 minutes and resuspend the cell pellet in fresh medium using the appropriate volume to achieve the correct seeding density.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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108CC5T-BU-4
Кат. номер 8062571-1VL 8062571-DNA-5UG 8062571-RNA-5UG Cell Line OriginMouse neuroblastoma x Rat glioma hybridCell Line DescriptionA bromodeoxyuridine resistant mutant of 108CC5 (Sigma Catalogue number 08062521), a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybridApplicationUsed in the generation of cell hybrids.Culture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridine.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required. Immediately after resuscitation a pre-centrifugation step to remove cryoprotectant (DMSO) is recommended i.e. pellet the cells by centrifugation at 100 x g for 5 minutes and resuspend the cell pellet in fresh medium using the appropriate volume to achieve the correct seeding density.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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10T
Кат. номер 99072801-RNA-5UG 99072801-DNA-5UG 99072801-1VL Cell Line OriginMouse embryo fibroblastCell Line DescriptionClone 8, contact inhibited, semi-normal.Culture MediumBME + 10% FBS.Subculture RoutineSplit sub confluent cultures (70-80%) 1:3 ie. seeding at 3x10,000 cells/cm2 using 0.25% trypsin/EDTA. 37°C; 5% CO2Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse embryo (c3h) growth mode Adherent karyotype Not specified morphology Fibroblast-like products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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11-4
Кат. номер 11033102-1VL 11033102-DNA-5UG Cell Line OriginHeLa, genetic modification, firefly luciferase reporter gene, HSV-thymidine kinase promoterCell Line Description11-4 is a HeLa cell line stably transfected with a firefly luciferase reporter gene under the control of the HSV-thymidine kinase promoter. 11-4 can be used to evaluate the non-specific transcriptional activity of chemicals.DNA ProfileSTR-PCR Data:
Amelogenin: X
CSF1PO: 9,10
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18Culture MediumDMEM, (D6546) + 2 mM L-Glutamine, (G7513) + 10% FBS / FCS, (F2442) + 16 μg/ml Blasticidin S HCLSubculture RoutineSplit sub-confluent cultures (70-80%) 1:4 to 1:10, i.e. seeding at 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human cervix growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice
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11-5.2.1.9
Кат. номер 89050905-DNA-5UG 89050905-RNA-5UG 89050905-1VL Cell Line OriginMouse NS1 x Mouse BALB/c spleenCell Line DescriptionThe antibody is directed against mouse I-Ak antigens. It does not react with the products of other I sub regions.ImmunogenCKB cells (H-2k)Culture MediumDMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma NS1 Reactivity Anti-I-Ak application(s) cell culture | mammalian: suitable isotype IgG2b shipped in dry ice storage temp. −196°C
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11-5F
Кат. номер 91121236-1VL 91121236-RNA-5UG 91121236-DNA-5UG Cell Line OriginMouse NS1 x Mouse BALB/c spleenCell Line DescriptionThe antibody is directed against desmoplakins 1 and 2 in mammalian epithelia, carcinoma and cultured cells.ImmunogenBovine desmoplakins (desmosomal glycoproteins 1 and 2) from nasal epidermisCulture MediumDMEM + 2mM glutamine + 20% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma NS1 Reactivity Anti-Desmoplakins application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. −196°C
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111 BLCL human cell line
Кат. номер 94031620-CDNA-20UL 94031620-DNA-5UG 94031620-1VL 94031620-RNA-5UG Cell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileNot specifiedSerological ProfileHLA A: 1 3
HLA C: 7 0
HLA B: 7 8
HLA DR: 2 3
HLA DQ: 1 2Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Caucasian gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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111A3
Кат. номер 85062504-DNA-5UG 85062504-RNA-5UG 85062504-1VL Cell Line OriginMouse 53B3 x Bovine Jersey Calf lymph nodeCell Line DescriptionNon Ig secretingImmunogenSheep red blood cellsApplicationThis line has been used as a HAT sensitive fusion partner in the production of heterohybridomasCulture MediumRPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°COther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Bovine Heterohybridoma ImmunoDon Jersey calf lymph node Myeloma 53B3 (mouse x bovine heteromyeloma) Reactivity Application: Fusion partner in the production of heterohybridomas application(s) cell culture | mammalian: suitable isotype Not specified shipped in dry ice storage temp. −196°C
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1156QE/8
Кат. номер 6072609-1VL 6072609-DNA-5UG 6072609-CDNA-20UL 6072609-RNA-5UG Cell Line OriginHuman embryonal carcinomaCell Line DescriptionA human embryonal carcinoma cell line established from a primary testicular tumour of a 22 year-old patient. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection.DNA ProfileSTR-PCR Data:
Amelogenin: X
CSF1PO: 11
D13S317: 8,9
D16S539: 11,14
D5S818: 11,12
D7S820: 9
THO1: 9.3
TPOX: 8
vWA: 16,17Culture MediumDMEM (High Glucose) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS). No sodium pyruvate.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at >6x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 10% CO2; 37°C. Cells need to be maintained at a high cell density.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human embryo (carcinoma) growth mode Not specified karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice
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1184
Кат. номер 90011883-RNA-5UG 90011883-DNA-5UG 90011883-CDNA-20UL 90011883-1VL Cell Line OriginHuman skin fibroblastCell Line DescriptionA thioguanine resistant derivative of MRC-5 CV1, selected with 5μg/ml 6-thioguanine.ApplicationGenetic studiesDNA ProfileSTR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10,11
THO1: 8
TPOX: 8
vWA: 15Culture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm3 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human skin growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C Узнать цену -
1221
Кат. номер 90011873-DNA-5UG 90011873-CDNA-20UL 90011873-RNA-5UG 90011873-1VL Cell Line OriginHuman skin fibroblast, thioguanine resistantCell Line DescriptionPrimary fibroblasts selected for resistance to 5μg/ml thioguanine.ApplicationGenetic studiesDNA ProfileSTR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 8
TPOX: 8
vWA: 15Culture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm3 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human skin growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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126 BLCL human cell line
Кат. номер 94031621-CDNA-20UL 94031621-RNA-5UG 94031621-1VL 94031621-DNA-5UG Cell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileNot specifiedSerological ProfileHLA A: 1 2
HLA C: 7 0
HLA B: 8 38
HLA DR: 3 6
HLA DQ: 1 2Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Caucasian gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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127 BLCL human cell line
Кат. номер 94031622-DNA-5UG 94031622-CDNA-20UL 94031622-1VL 94031622-RNA-5UG Cell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileNot specifiedSerological ProfileHLA A: 1 11
HLA C: 7 0
HLA B: 8 18
HLA DR: 3 11
HLA DQ: 2 3Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Caucasian gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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129 BLCL human cell line
Кат. номер 94031623-RNA-5UG 94031623-DNA-5UG 94031623-CDNA-20UL 94031623-1VL Cell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileNot specifiedSerological ProfileHLA A: 1 3
HLA C: 7 0
HLA B: 7 8
HLA DR: 2 4
HLA DQ: 1 3Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Caucasian gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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130 BLCL human cell line
Кат. номер 94031624-DNA-5UG 94031624-CDNA-20UL 94031624-1VL 94031624-RNA-5UG Cell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileNot specifiedSerological ProfileHLA A: 2 0
HLA C: 7 0
HLA B: 8 38
HLA DR: 3 6
HLA DQ: 1 2Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Caucasian gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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1301 Cell Line human
Кат. номер 1051619-RNA-5UG 1051619-CDNA-20UL 1051619-1VL Cell Line OriginHuman T-cell leukaemiaCell Line Description1301 is a sub-line of CCRF-CEM, Sigma catalogue no. 85112105. It has unusually long telomeres, useful in tests where telomere length is determined, where it can be used as control cells with known telomere length. This cell line has been recommended as a control cell line for use with the Telomere PNA Kit/FITC for flow cytometry from DakoCytomation.ApplicationInternal control cell in telomere measurements performed by flow cytometry.Culture MediumRPMI 1640 (R0883), + 2mM L-Glutamine (G7513), + 10% FBS / FCS (F2442),Subculture RoutineMaintain cultures between 3-9 x 100,000 cells/ml; 37°C; 5% CO2.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood growth mode Suspension karyotype Tetraploid morphology Lymphoblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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1306
Кат. номер 90011887-CDNA-20UL 90011887-RNA-5UG 90011887-1VL 90011887-DNA-5UG Cell Line OriginHuman skin fibroblastCell Line DescriptionA HPRT- derivative of the SV40 transformed human fibroblast line GM0637. The cells were selected in 5μg/ml thioguanine and are useful for transfection experiments.ApplicationTransfection studiesDNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 13
D16S539: 12,13
D5S818: 11
D7S820: 10
THO1: 7,9
TPOX: 9,11
vWA: 16,17Culture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human skin growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C Узнать цену -
131 BLCL human cell line
Кат. номер 94031625-DNA-5UG 94031625-1VL 94031625-RNA-5UG 94031625-CDNA-20UL Cell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileNot specifiedSerological ProfileHLA A: 2 28
HLA C: 3 7
HLA B: 44 62
HLA DR: 7 0
HLA DQ: 2 0Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Caucasian gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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1321N1 Cell Line human
Кат. номер 86030402-CDNA-20UL 86030402-RNA-5UG 86030402-1VL Cell Line OriginHuman brain astrocytomaCell Line Description1321N1 is a human astrocytoma cell line isolated in 1972 as a sub clone of the cell line 1181N1 which in turn was isolated from the parent line U-118 MG (one of a number of cell lines derived from malignant gliomas by J Ponten .1321N1 has been shown to have very similar STR profile data to U-118 MG.ApplicationHuman 1321N1 Cell Line has been used to study how the multiscale morphology of functional thin films affects the in vitro behavior of human neural astrocytoma 1321N1 cells. It has also been used to study the expression and reactivity of Caytaxin protein.DNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 12,13
D5S818: 11
D7S820: 9
THO1: 6
TPOX: 8
vWA: 18Culture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human brain (astrocytoma) growth mode Adherent karyotype Not specified morphology Glial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
133 BLCL human cell line
Кат. номер 94031626-1VL 94031626-RNA-5UG 94031626-DNA-5UG 94031626-CDNA-20UL Cell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileNot specifiedSerological ProfileHLA A: 1 2
HLA C: 3 7
HLA B: 8 60
HLA DR: 3 11
HLA DQ: 1 2Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Caucasian gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
136 human cell line
Кат. номер 94071418-DNA-5UG 94071418-1VL 94071418-CDNA-20UL 94071418-RNA-5UG Cell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 2
Cw: 304
B: 4005 48/40Serological ProfileHLA A:
HLA B: 4005
Bw4/bw6: 6Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Native American gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
13C4
Кат. номер 95032701-RNA-5UG 95032701-1VL 95032701-DNA-5UG Cell Line OriginMouse Sp2/0 Ag-14 x Mouse BALB/c spleenCell Line DescriptionThe antibody is directed against Shigella toxin and neutralises Shigella toxin activities from Shigella dysenteriae Type 1 and S. flexneri. It also reacts with the subunit of SLTI and neutralises the Shigella-like activities of E.coli, Vibrio cholerae, V.parahaemolyticus and Salmonella typhimurium.ImmunogenPurified Shigella-like toxin from E.coli H30ApplicationStudy of shiga toxins.Culture MediumDMEM + 2mM glutamine + 0.15mg/ml oxaloacetate + 0.2U/ml Bovine Insulin + 0.05mg Sodium Pyruvate (NaP) + 5-10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma Sp2/0 Ag-14 Reactivity Anti-Shigella-like Toxin I (SLTI) application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. −196°C
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1411H
Кат. номер 6011805-1VL 6011805-DNA-5UG 6011805-RNA-5UG 6011805-CDNA-20UL Cell Line OriginHuman yolk sac carcinomaCell Line DescriptionA non seminomatous germ cell carcinoma derived cell line. A yolk sac carcinoma cell line derived from a testicular teratomacarcinoma. This cell line is tumourigenic in nude mice.Culture MediumDMEM (High Glucose) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). No sodium pyruvate.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 10% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human embryo growth mode Adherent karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
142BR
Кат. номер 90011806-RNA-5UG 90011806-1VL 90011806-DNA-5UG 90011806-CDNA-20UL Cell Line OriginHuman skin fibroblastCell Line DescriptionEstablished from a biopsy of a 51 year old normal male. The cells have been used in radiation survival studies.ApplicationDNA repair studiesCulture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).Subculture RoutineSplit confluent cultures 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Allow cultures to reach confluency before splitting and do not split more than 1:4.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human skin growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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143B
Кат. номер 91112502-RNA-5UG 91112502-1VL 91112502-CDNA-20UL 91112502-DNA-5UG Cell Line OriginHuman bone osteosarcoma TK-Cell Line DescriptionCells are thymidine kinase deficient (TK-) and resistant to BUdR.143B and HOS (Sigma Catalogue number 87070202) originated from the same host: 143B is TK- and originated indirectly from HOS which is TK+.ApplicationTumourigenicity studiesDNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18Culture MediumEMEM (EBSS) + 2mM Glutamine + 15ug/ml Bromo-deoxy Uridine (BUdR) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human bone growth mode Adherent karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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145 BLCL human cell line
Кат. номер 94031627-RNA-5UG 94031627-1VL 94031627-CDNA-20UL 94031627-DNA-5UG Cell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileNot specifiedSerological ProfileHLA A: 3 30
HLA C: 6 7
HLA B: 7 13
HLA DR: 2 7
HLA DQ: 1 2Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Caucasian gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
Safety InformationRIDADR NONH for all modes of transport WGK Germany 3 Узнать цену
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