In Vitro Toxicology Assay Kit, MTT based TOX1-1KT

NACRES:

NA.84

This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results. The key component is (3- [4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) or MTT. Solutions of MTT, dissolved in medium or balanced salt solutions without phenol red, are yellowish in color. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple formazan crystals which are insoluble in aqueous solutions. Absorbance of converted dye is measured at a wavelength of 570nm.
In Vitro Toxicology Assay Kit, MTT based has been used to perform an (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay to measure the cytotoxicity of gold nanoparticles (AuNP). It has also been used to examine cell viability in SH-SY5Y cultures in response to 6-hydroxydopamine (6-OHDA) treatment.
Conversion of MTT to a water-insoluble colored formazan derivative which is then solubilized in acidic isopropanol.

Quality Level	200
usage	1 kit sufficient for 1,000 tests
packaging	pkg of 1 kit
storage condition	dry at room temperature
application(s)	cell analysis: suitable, detection: suitable
max	570 nm
detection method	colorimetric
storage temp.	2-8°C

Pictograms	GHS02,GHS05,GHS07,GHS08
Signal Word	Danger
Hazard Statements	H225 - H314 - H335 - H336 - H341 - H412
Precautionary Statements	P210 - P261 - P273 - P280 - P305 + P351 + P338 - P310
RIDADR	UN 3316 9
Flash Point(F)	53.6 °F
Flash Point(C)	12 °C