- Производитель:
- Roche
Кат. номер |
5015944001 |
11644807001 |
Contents
Ready-to-use solution, containing WST-1 and an electron coupling reagent.
Colorimetric assay (WST-1 based) for the nonradioactive quantification of cell proliferation, cell viability, and cytotoxicity.
- Analysis of cytotoxic and cytostatic compounds, such as anti-cancer drugs and other pharmaceutical compounds
- Assessment of growth inhibitory antibodies and physiological mediators
- Convenient: Benefit from a ready-to-use reagent.
- Safe and Easy: Eliminate radioactive isotopes, washing steps, and additional reagents.
- Accurate: The absorbance obtained strongly correlates to the cell number.
- Sensitive: Detect low cell numbers.
- Fast: Process a large number of samples using a multi-well ELISA reader.
Cells grown in a 96-well tissue culture plate are incubated with the WST-1 reagent for 0.5 - 4 hours. After this incubation period, the formazan dye formed is quantitated with a scanning multi-well spectrophotometer (ELISA reader). The measured absorbance directly correlates to the number of viable cells.
Cell proliferation and viability assays are of particular importance for routine applications in cell biology. Tetrazolium salts (e.g., MTT, XTT, WST-1) are particularly useful for this type of analysis. Tetrazolium salts are cleaved to formazan by the succinate-tetrazolium reductase system (EC 1.3.99.1) which belongs to the respiratory chain of the mitochondria, and is only active in metabolically intact cells.
Using the 100 µl/well cell culture volume, one vial will be sufficient to perform 2500 tests (25 microplates).
Note: If the cells are cultured in 200 µl/well, add 20 µl/well Cell Proliferation Reagent WST-1.
Storage conditions (working solution): 2 to 8 °C
Note: When precipitates or turbidity are observed upon thawing, warm up the solution to 37 °C for 2 to 10 minutes and agitate to dissolve the precipitates.Centrifugation is not recommended because the working concentration would decrease. After being dissolved, the WST-1 reagent can be used without any limitations. Please store as follows:
Once thawed, store at 2 to 8 °C, protected from light, for up to four weeks. However please note that the solution may become viscous. If so, warm up the solution to 37 °C for 2 to 10 minutes as described above.
Long storage
For long storage, aliquots of WST-1 can be stored in plastic tubes at -15 to -25 °C until the expiry date.
Quality Level | 100 |
form | solution |
usage | sufficient for 2,500 tests (11644807001), sufficient for 800 tests (05015944001) |
packaging | bottle of 25 mL (11644807001), bottle of 8 mL (05015944001) |
Торговая марка | Roche |
storage condition | protect from light |
application(s) | cell analysis: suitable, detection: suitable, protein quantification: suitable |
optimum pH | 8.0(for physiological conditions our product is buffered pH 7.3) |
max | 440-480 nm |
detection method | colorimetric |
shipped in | dry ice |
storage temp. | 20°C |
RIDADR | NONH for all modes of transport |
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