Catalase from bovine liver

CAS Number:	9001-05-2
Enzyme Commission (EC) Number:	1.11.1.6 ( BRENDA | IUBMB )
EC Number:	232-577-1
MDL number:	MFCD00081483
eCl@ss:	32160410
NACRES:	NA.75

Кат. номер

C1345-10G

C1345-1G

C1345-100G

CAT (catalase) is an endogenous antioxidant enzyme, thus conferring protection to cells against damage by ROS (reactive oxygen species). In humans, this gene is localized to chromosome 11p13, which is composed of 12 introns and 13 exons.
Catalase from bovine liver is used for the following applications:

• Gel filtration
• Analytical filtration on Ultrogel and glycerol gradient centrifugation
• Size exclusion chromatography (HPLC)-HPLC analysis of estrogen receptor
• Enzyme assays
• Alcohol oxidase activity

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.
Packaging
1, 10, 100 g in poly bottle
Biochem/physiol Actions
This product doesn not need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate.
Catalase catalyzes the degradation of hydrogen peroxide into water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor.
Components
Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.
Caution
Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.
Unit Definition
One unit will decompose 1.0 µmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.
Preparation Note
This product is a powder suitable for cell culture with activity at 2,000-5,000 units/mg. The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0.

Quality Level	200
biological source	bovine liver
product line	BioReagent
form	powder
specific activity	2,000-5,000 units/mg protein
mol wt	tetramer ~250 kDa
application(s)	cell culture | mammalian: suitable
isoelectric point	5.4
UniProt accession no.	P00432,
shipped in	wet ice
storage temp.	20°C
Gene Information	cow ... CAT(280743)

pictograms	GHS08
signalword	Danger
hcodes	H334
pcodes	P261 - P284 - P304 + P340 + P312 - P501
Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable