Animal Free Collagenase/Dispase Blend II SCR140

eCl@ss:

32160405

Product Source: Isolated and purified from Clostridium histolyticum and Bacillus polymyxa cultures devoid of animal containing reagents.
This product is manufactured in an ISO9001:2008 certified enviroment.
Crude collagenases are widely used in enzymatic primary cell isolation and tissue dissociation procedures. Most researchers employ either crude collagenase preparations such as Types I, II, III, and IV or chromatographically purified collagenase. Dispase is especially suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes. EMD Millipore’s Animal Free Collagenase/Dispase Blend II is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired. The product is prepared and purified from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens, such as BSE,TSE and other mammalian viruses, must be prevented.
Research Category
Stem Cell Research
Research Sub Category
Enzymes & Biochemistry
Animal Free Collagenase/Dispase Blend II is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired.
Product is diafiltered with water, filtered through a 0.22 micron membrane and lyophilized in vials.
Store at 2-8°C. Further dilutions should be made into appropriate buffer and/or medium to which protein has been added, such as DMEM/1% Human Serum Albumin or equivalent.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100,
caseinase activity	≥2000 caseinase units/mg
clostripain activity	≤5.0 clostripain units/mg
collagenase activity	≥12,500 U/vial
trypsin activity	≤0.5 trypsin units/mg
application(s)	cell analysis: suitable (cell harvesting), cell culture | mammalian: suitable
shipped in	wet ice

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable