Biological Buffers
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SuRE/Cut™ Buffer H 11417991001
General descriptionThe SuRE/Cut Buffer System consists of five optimized incubation buffers, 10x concentrated, for DNA restriction digest. Activity of all restriction enzymes has been determined in each buffer to select 100% activity or to calculate activity in double-digests.
ApplicationThe SuRE/Cut Buffer H has been used for digestion of genomic DNA using the restriction enzyme XbaI. It has been used for digestion of PCR products and plasmid using EcoRI and XhoI.Other NotesFor life science research only. Not for use in diagnostic procedures.Legal InformationSuRE/Cut is a trademark of RocheПараметрыform solution Quality Level 100, packaging pkg of 5 x 1 mL Торговая марка Roche shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F No data available Flash Point C No data available Узнать цену -
SuRE/Cut™ Buffer M 11417983001
General descriptionThe SuRE/Cut Buffer System consists of five optimized incubation buffers, 10x concentrated, for DNA restriction digest. Activity of all restriction enzymes has been determined in each buffer to select 100% activity or to calculate activity in double-digests.ApplicationIt was used in the preparation of hot probe (C-rich).Other NotesFor life science research only. Not for use in diagnostic procedures.Legal InformationSuRE/Cut is a trademark of RocheПараметрыform solution Quality Level 100, packaging pkg of 5 x 1 mL (10x concentrated) Торговая марка Roche shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F does not flash Flash Point C does not flash Узнать цену -
TAPS
Empirical Formula (Hill Notation): C7H17NO6S CAS Number: 29915-38-6 Molecular Weight: 243.28 Beilstein/REAXYS Number: 2452420 EC Number: 249-954-1 MDL number: MFCD00007538 PubChem Substance ID: 24900259 NACRES: NA.25
Кат. номер T5130-10KG T5130-250G T5130-5KG T5130-1KG T5130-PM T5130-100G T5130-25G T5130-50KG T5130-BR ПараметрыQuality Level 400, assay ≥99.5% (titration) form crystalline powder useful pH range 7.7 - 9.1 pKa A 8.4 mp 230-235 °C (dec.) solubility water: 0.5 g/mL, clear, colorless Featured Industry Diagnostic Assay Manufacturing SMILES string OCC(CO)(CO)NCCCS(O)(=O)=O InChI 1S/C7H17NO6S/c9-4-7(5-10,6-11)8-2-1-3-15(12,13)14/h8-11H,1-6H2,(H,12,13,14) InChI key YNLCVAQJIKOXER-UHFFFAOYSA-N
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F 230.0 °F - closed cup Flash Point C > 110 °C - closed cup Узнать цену -
TAPS sodium salt
Empirical Formula (Hill Notation): C7H16NNaO6S CAS Number: 91000-53-2 Molecular Weight: 265.26 MDL number: MFCD00069903 PubChem Substance ID: 24899903 NACRES: NA.25
Кат. номер T0647-100G T0647-25G T0647-500G ПараметрыQuality Level 200, assay ≥99% (titration) form crystalline powder impurities ≤1% water content (Karl Fischer) useful pH range 7.7 - 9.1 pKa (25 °C) 8.4 solubility water: 0.33 g/mL, clear to slightly hazy, colorless cation traces Pb: ≤5 ppm Featured Industry Diagnostic Assay Manufacturing SMILES string [Na+].OCC(CO)(CO)NCCCS([O-])(=O)=O InChI 1S/C7H17NO6S.Na/c9-4-7(5-10,6-11)8-2-1-3-15(12,13)14;/h8-11H,1-6H2,(H,12,13,14);/q;+1/p-1 InChI key FEGYIWVHCSRXCG-UHFFFAOYSA-M
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TAPSO
Empirical Formula (Hill Notation): C7H17NO7S CAS Number: 68399-81-5 Molecular Weight: 259.28 Beilstein/REAXYS Number: 2113949 EC Number: 269-993-8 PubChem Substance ID: 24900590 NACRES: NA.25
Кат. номер T9269-100G T9269-25G Packaging100 g in poly bottleCautionAccording to Ferreira, et al., TAPSO can have a strong interaction with iron in solution. Based on this citation, it is recommended to use plastic-coated or PTFE-coated utensils when working with this chemical.ПараметрыQuality Level 200, assay ≥99% (titration) form crystalline powder useful pH range 7.0 - 8.2 pKa (25 °C) 7.6 solubility warm water: 0.333 g/mL, clear to slightly hazy, colorless Featured Industry Diagnostic Assay Manufacturing SMILES string OCC(CO)(CO)NCC(O)CS(O)(=O)=O InChI 1S/C7H17NO7S/c9-3-7(4-10,5-11)8-1-6(12)2-16(13,14)15/h6,8-12H,1-5H2,(H,13,14,15) InChI key RZQXOGQSPBYUKH-UHFFFAOYSA-N
Safety Informationpictograms GHS07 signalword Warning hcodes H315 - H319 - H335 pcodes P302 + P352 - P305 + P351 + P338 Target Organs Respiratory system Personal Protective Equipment dust mask type N95 (US),, Eyeshields,, Gloves, RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
TES
Empirical Formula (Hill Notation): C6H15NO6S CAS Number: 7365-44-8 Molecular Weight: 229.25 Beilstein/REAXYS Number: 1957061 EC Number: 230-906-3 MDL number: MFCD00007532 PubChem Substance ID: 24899960 NACRES: NA.25
Кат. номер T1375-10G T1375-100G T1375-1KG T1375-25KG T1375-5KG T1375-25G T1375-500G ApplicationTES has been used in TES buffer to determine anti-collagenase activity of Marula stems. It has also been used in buffers for cell culture medium.Packaging25, 100, 500 g in poly bottle
5 kg in poly drum
1 kg in poly bottle
25 kg in fiber drumПараметрыQuality Level 200, assay 99% (titration) form crystalline powder useful pH range 6.8 - 8.2 pKa (25 °C) 7.5 mp ~225 °C (dec.) solubility H2O: 25 g plus 50 mL, clear, colorless Featured Industry Diagnostic Assay Manufacturing SMILES string OCC(CO)(CO)NCCS(O)(=O)=O InChI 1S/C6H15NO6S/c8-3-6(4-9,5-10)7-1-2-14(11,12)13/h7-10H,1-5H2,(H,11,12,13) InChI key JOCBASBOOFNAJA-UHFFFAOYSA-N
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TES hemisodium salt
Empirical Formula (Hill Notation): C6H14.5NO6S · 0.5 Na Molecular Weight: 240.24 NACRES: NA.25
Кат. номер T1030-100G T1030-25G Параметрыassay ≥99% Quality Level 100, form powder or crystals useful pH range 6.8 - 8.2 pKa (25 °C) 7.5 solubility water: 0.25 g/mL, clear, colorless Featured Industry Diagnostic Assay Manufacturing SMILES string [Na+].OCC(CO)(CO)NCCS(O)(=O)=O.OCC(CO)(CO)NCCS([O-])(=O)=O
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TES sodium salt
Empirical Formula (Hill Notation): C6H14NNaO6S CAS Number: 70331-82-7 Molecular Weight: 251.23 MDL number: MFCD00065482 PubChem Substance ID: 24899912 NACRES: NA.25
Кат. номер T0772-100G T0772-25G T0772-500G ПараметрыQuality Level 200, assay ≥99% (titration) form crystalline powder impurities ≤7% water content (Karl Fischer) useful pH range 6.8 - 8.2 pKa (25 °C) 7.5 solubility water: 0.333 g/mL, clear to slightly hazy, colorless cation traces Pb: ≤5 ppm Featured Industry Diagnostic Assay Manufacturing SMILES string [Na+].OCC(CO)(CO)NCCS([O-])(=O)=O InChI 1S/C6H15NO6S.Na/c8-3-6(4-9,5-10)7-1-2-14(11,12)13;/h7-10H,1-5H2,(H,11,12,13);/q;+1/p-1 InChI key GUXMFAHOYNRHBI-UHFFFAOYSA-M
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
TISAB IV solution
NACRES: NB.61
Кат. номер 34574-VAR 34574-2.5L 34574-500ML 34574-6X500ML 34574-4X2.5L General descriptionTotal ionic strength adjustment buffers (TISAB), is generally used for the microdetermination of fluorides.
Visit our Sensor Applications portal, to learn more.ApplicationTISAB IV was used as a metal ion complexing and ionic concentration adjustment agent, in an experiment investigating the increase in fluoride release, from glass ionomer cement, after coating the discs of glass ionomer with 10% silver fluoride (AgF).Other NotesImportant notice
The article number 34574-4X2.5L will be discontinued. Please order the single bottle 34574-2.5L which is physically identical with the same exact specifications.Параметрыgrade for determination of fluoride Quality Level 100, form liquid pH 5.0-5.5
Safety Informationpictograms GHS05 signalword Warning hcodes H290 - H315 - H319 pcodes P302 + P352 - P305 + P351 + P338 Personal Protective Equipment Faceshields,, Gloves,, Goggles,, type ABEK (EN14387) respirator filter, RIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
TNT buffer solution
NACRES: NA.25
Кат. номер 89554-VAR 89554-100ML 89554-500ML ApplicationTRIS NaCl Tween 20 (TNT) buffer solution, pH 8.0 may be used in molecular biology as a washing buffer and potentially as an elution buffer for immuno micro sensors.Legal InformationTWEEN is a registered trademark of Croda International PLCПараметрыgrade for molecular biology Quality Level 100, product line BioUltra form liquid impurities DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected pH 8.0±0.2 (25 °C) cation traces Al: 1 mg/kg, Ba: 1 mg/kg, Bi: 1 mg/kg, Ca: 5 mg/kg, Cd: 1 mg/kg, Co: 1 mg/kg, Cr: 1 mg/kg, Cu: 1 mg/kg, Fe: 1 mg/kg, K: 20 mg/kg, Li: 1 mg/kg, Mg: 1 mg/kg, Mn: 1 mg/kg, Mo: 1 mg/kg, Ni: 1 mg/kg, Pb: 1 mg/kg, Sr: 1 mg/kg, Zn: 1 mg/kg neat UV absorption : 260 nm Amax: 0.04, : 280 nm Amax: 0.03
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves, RIDADR NONH for all modes of transport WGK Germany nwg Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Tricine
Empirical Formula (Hill Notation): C6H13NO5 Linear Formula: (HOCH2)3CNHCH2CO2H CAS Number: 5704-04-1 Molecular Weight: 179.17 Beilstein/REAXYS Number: 1937804 EC Number: 227-193-6 MDL number: MFCD00004277 PubChem Substance ID: 24899884 NACRES: NA.25
Кат. номер T0377-5KG T0377-100G T0377-25KG T0377-250G T0377-25G T0377-1KG Packaging5, 25 kg in poly drum
25, 100, 250 g in poly bottle
1 kg in poly bottleApplicationBuffer component for separation of low molecular weight peptides.ПараметрыQuality Level 400, assay ≥99% (titration) form crystalline powder useful pH range 7.4 - 8.8 pKa (25 °C) 8.1 mp 187 °C solubility water: 0.25 g/mL, clear, colorless Featured Industry Diagnostic Assay Manufacturing SMILES string OCC(CO)(CO)NCC(O)=O InChI 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12) InChI key SEQKRHFRPICQDD-UHFFFAOYSA-N
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Triethanolamine Buffer solution T0449-120ML
Packaging120 mL in glass bottleComponentsContains triethanolamine, 0.2 mol/L, magnesium ions and EDTA, pH 8.0 at 25 °C. Less than 0.1% sodium azide added as a preservative.Параметрыform liquid Quality Level 200, Featured Industry Diagnostic Assay Manufacturing storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Triethylamine
Empirical Formula (Hill Notation): C6H15N Linear Formula: (C2H5)3N CAS Number: 121-44-8 Molecular Weight: 101.19 Beilstein/REAXYS Number: 605283 EC Number: 204-469-4 MDL number: MFCD00009051 PubChem Substance ID: 57653384 NACRES: NA.25
Кат. номер 90335-1L 90335-100ML 90335-250ML 90335-6X1L 90335-6X250ML ApplicationTriethylamine, a mobile-phase modifier in the RP-HPLC separation of acidic, basic and neutral molecules, improves the resolution of amino acids and amino acid amides by HPLC by suppressing tailing and the stability of drugs and other compounds.Other NotesComponent of volatile buffer system in ion-exchange HPLC of oligonucleotidesПараметрыQuality Level 200, vapor density 3.5 (vs air) vapor pressure 51.75 mmHg ( 20 °C) product line BioUltra assay 99.5% (GC) form liquid autoignition temp. 593 °F shelf life 36 mo. expl. lim. 8 % application(s) ion chromatography: suitable impurities insoluble matter, passes filter test, 0.5% water evapn. residue 0.003% refractive index n20/D 1.401 (lit.) pH 12.7 (15 °C, 100 g/L) bp 88.8 °C (lit.) mp 115 °C (lit.) density 0.726 g/mL at 25 °C (lit.) cation traces Al: 0.2 mg/kg, Ba: 0.1 mg/kg, Bi: 0.1 mg/kg, Ca: 0.5 mg/kg, Cd: 0.05 mg/kg, Co: 0.02 mg/kg, Cr: 0.02 mg/kg, Cu: 0.02 mg/kg, Fe: 0.1 mg/kg, K: 0.5 mg/kg, Li: 0.1 mg/kg, Mg: 0.1 mg/kg, Mn: 0.02 mg/kg, Mo: 0.02 mg/kg, Na: 0.5 mg/kg, Ni: 0.02 mg/kg, Pb: 0.1 mg/kg, Sr: 0.1 mg/kg, Zn: 0.1 mg/kg absorption cut-off at 278 nm storage temp. 2-8°C SMILES string CCN(CC)CC InChI 1S/C6H15N/c1-4-7(5-2)6-3/h4-6H2,1-3H3 InChI key ZMANZCXQSJIPKH-UHFFFAOYSA-N
Safety Informationpictograms GHS02,GHS05,GHS06 signalword Danger hcodes H225 - H302 - H311 + H331 - H314 - H335 pcodes P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 Target Organs Respiratory system Personal Protective Equipment Faceshields,, Gloves,, Goggles,, type ABEK (EN14387) respirator filter, RIDADR UN1296 - class 3 - PG 2 - Triethylamine WGK Germany WGK 1 Flash Point F 12.2 °F Flash Point C -11 °C Узнать цену -
Triethylammonium acetate buffer
PubChem Substance ID: 24889353 NACRES: NA.25
Кат. номер 90358-INTR 90358-2.5L 90358-100ML 90358-500ML 90358-4X2.5L 90358-6X500ML 90358-6X100ML ApplicationTriethylammonium acetate buffer has been used for visualizing and quantifying single mRNA molecules in mammalian (mouse and human) tissues.ПараметрыQuality Level 200, form liquid shelf life limited shelf life, expiry date on the label concentration ~1.0 M in H2O refractive index n20/D 1.357 pH 7.0 density 1.002 g/mL at 20 °C Featured Industry Diagnostic Assay Manufacturing storage temp. 2-8°C
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves, RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Triethylammonium bicarbonate buffer
CAS Number: 15715-58-9 Beilstein/REAXYS Number: 3624751 MDL number: MFCD00466938 PubChem Substance ID: 24900453 NACRES: NA.25
Кат. номер T7408-500ML T7408-100ML ApplicationTriethylammonium bicarbonate buffer is used in ion-exchange chromatography and electrophoresis.
It has also been used:- in trypsin digestion
- as a component of lysis buffer
- to adjust the pH of buffer for protein quantification
Buffer for use in ion-exchange chromatography and electrophoresis.Biochem/physiol ActionsTriethylammonium bicarbonate buffer is useful in oligonucleotides purification for electrospray mass spectrometry analysis. It is also utilized in trypsin digestion buffers.Параметрыform liquid Quality Level 200, concentration 1.0 M pH 8.5±0.1 density 1.02 g/mL at 25 °C (lit.) Featured Industry Diagnostic Assay Manufacturing storage temp. 2-8°C SMILES string OC(O)=O.CCN(CC)CC InChI 1S/C6H15N.CH2O3/c1-4-7(5-2)6-3;2-1(3)4/h4-6H2,1-3H3;(H2,2,3,4) InChI key AFQIYTIJXGTIEY-UHFFFAOYSA-N
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type ABEK (EN14387) respirator filter, RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Triethylammonium phosphate solution
MDL number: MFCD00067475 PubChem Substance ID: 57653391 NACRES: NA.25
Кат. номер 90362-500ML 90362-100ML Biochem/physiol ActionsTriethylammonium phosphate (TEAP) buffers are highly efficient solvents, that can be used for reversed-phase high-performance liquid chromatography (RP-HPLC).Other NotesMobile phase (15 mM) for reversed phase HPLC of amino acids, peptides and proteinsПараметрыproduct line BioXtra Quality Level 200, form liquid concentration 1 M in H2O refractive index n20/D 1.360 pH 2.9-3.1 (25 °C) density 1.05 g/mL at 20 °C anion traces chloride (Cl-): 50 mg/kg, sulfate (SO42-): 50 mg/kg cation traces Ca: 5 mg/kg, Cd: 1 mg/kg, Co: 1 mg/kg, Cr: 1 mg/kg, Cu: 1 mg/kg, Fe: 1 mg/kg, K: 20 mg/kg, Mg: 1 mg/kg, Mn: 1 mg/kg, Na: 20 mg/kg, Ni: 1 mg/kg, Pb: 1 mg/kg, Zn: 1 mg/kg Featured Industry Diagnostic Assay Manufacturing SMILES string OP(O)(O)=O.OP(O)(O)=O.CCN(CC)CC InChI 1S/C6H15N.H3O4P/c1-4-7(5-2)6-3;1-5(2,3)4/h4-6H2,1-3H3;(H3,1,2,3,4) InChI key UNXNGGMLCSMSLH-UHFFFAOYSA-N
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves, RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
TRIS acetate – EDTA buffer substance
NACRES: NA.25
Кат. номер 93295-1L 93295-100ML 93295-5L Reconstitutiondissolution to the indicated volume yields a 10x concentrated solution (0.4 M TRIS acetate, pH ~8.5, 10 mM EDTA.Na2)ПараметрыQuality Level 100, grade for molecular biology product line BioReagent form powder quality 10x concentrate impurities DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected color white pH 8.2-8.5 solubility water: 64.2 g/L, clear, colorless
Safety InformationPersonal Protective Equipment dust mask type N95 (US),, Eyeshields,, Gloves, RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Tris Acetate-EDTA buffer
MDL number: MFCD00236357 PubChem Substance ID: 329826908 NACRES: NA.25
Кат. номер T7688-20ML-PW T9650-4L-PW T7688-100ML-PW T9650-PH T9650-1L-PW T9650-4L T9650-1L T9650-6X1L ApplicationTris Acetate-EDTA buffer has been used for the preparation of agarose gel during DNA agarose gel electrophoresis.
TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1x TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1x TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.Other Notes0.4 M Tris acetate, pH approx. 8.3, containing 0.01 M EDTA.Preparation NotePrepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).
Solution prepared with 18 megohm waterПараметрыQuality Level 200, grade for molecular biology sterility non-sterile; 0.2 μm filtered product line BioReagent form solution suitability suitable for gel electrophoresis (after dilution to working concentration) SMILES string CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O InChI 1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4) InChI key HGEVZDLYZYVYHD-UHFFFAOYSA-N
Safety Informationpictograms GHS08 signalword Warning hcodes H373 pcodes P260 - P313 - P501 Target Organs Respiratory Tract RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Tris Acetate-EDTA Buffer 50X concentrate
NACRES: NA.25
Кат. номер SRE0033-PH SRE0033-1L SRE0033-1L-PW ApplicationRunning buffer for nucleic acid electrophoresis.Physical form2M TRIS acetate and 0.05M EDTA in water. Solution is 0.2 µm filtered.
Dilute with water to 1X.ПараметрыQuality Level 500,, 600, form liquid Featured Industry Diagnostic Assay Manufacturing storage temp. room temp
Safety Informationpictograms GHS02,GHS07 signalword Warning hcodes H226 - H315 - H319 pcodes P210 - P302 + P352 - P305 + P351 + P338 RIDADR NONH for all modes of transport WGK Germany WGK 1 Узнать цену -
Tris Acetate-EDTA buffer Concentrate
NACRES: NA.25
Кат. номер 67996-10L-F 67996-VAR-F General descriptionTBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double stranded DNA runs faster with TAE buffer. This 50x concentrate can be easily diluted with molecular biology grade water to 1x before use.ApplicationTris Acetate-EDTA buffer concentrate is suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. It is especially useful for double stranded DNA separations.ComponentsThe TAE 50x liquid concentrate contains 2M TRIS acetate and 0.05M EDTA in water.Параметрыgrade for molecular biology form solution quality 50x Concentrate impurities DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected pH 8.3±0.2 (25 °C) density 1.09 g/mL at 20 °C storage temp. room temp
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
TRIS borate – EDTA buffer solution
eCl@ss: 32129211 NACRES: NA.25
Кат. номер 93290-1L 93290-INTR 93290-5L General descriptionTBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. The TBE solution is effective under slightly basic conditions, which keeps DNA deprotonated , water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).ApplicationSuitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures.ComponentsThe TBE 10x liquid contains 1.3M TRIS, 450 mM boric acid and 25 mM EDTA in water.ПараметрыQuality Level 100, grade for molecular biology description long run, concentrate product line BioReagent form solution quality 10x concentrate, ready-to-use solution impurities DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected pH 8.6-9.0 storage temp. room temp
Safety Informationpictograms GHS08 signalword Danger hcodes H360FD pcodes P201 - P202 - P280 - P308 + P313 - P405 - P501 Personal Protective Equipment Eyeshields,, Gloves,, type ABEK (EN14387) respirator filter, RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
TRIS borate – EDTA buffer substance
NACRES: NA.25
Кат. номер 93309-1L 93309-5X1L 93309-5L General descriptionTBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble and protected from degradation. This powder should be reconstituted in molecular biology grade water before use.ApplicationTRIS borate – EDTA buffer substance is suitable for use as running buffer for agarose gel electrophoresis and nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures.ComponentsThe final 5x TBE liquid contains 0.445M TRIS borate (pH 8.3) and 0.01M EDTA in water.ПараметрыQuality Level 100, grade for molecular biology product line BioReagent form powder quality 5x concentrate impurities DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected color white pH 8.1-8.5 storage temp. room temp
Safety Informationpictograms GHS07,GHS08 signalword Danger hcodes H315 - H319 - H335 - H360FD pcodes P201 - P261 - P305 + P351 + P338 - P308 + P313 Personal Protective Equipment Eyeshields,, Gloves,, type P1 (EN143) respirator filter,, type P3 (EN 143) respirator cartridges, RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
TRIS borate-EDTA buffer concentrate, long run 51309-10L-F
MDL number: MFCD00217350 NACRES: NA.25 General descriptionTBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated , water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).ApplicationSuitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures.ComponentsThe TBE 10x concentrate contains 1.3M TRIS, 450 mM boric acid and 25 mM EDTA in water.ПараметрыQuality Level 100, grade for molecular biology product line BioReagent form solution quality 10x concentrate, ready-to-use solution impurities DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected storage temp. room temp
Safety Informationpictograms GHS08 signalword Danger hcodes H360FD pcodes P201 - P202 - P280 - P308 + P313 - P405 - P501 RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Tris Buffered Saline 94158-10TAB
MDL number: MFCD00132476 NACRES: NA.25 ApplicationTrizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris Buffered Saline, pH 7.6 may be used as a sample diluent, phase separation media or placebo.Reconstitution1 tablet dissolved in 500 mL of deionized water yields:
0.15 M sodium chloride; 0.050 M TRIS-HCl buffer; pH 7.6 at 25°CПараметрыproduct line BioUltra Quality Level 200, form tablet (for 500 mL) pH 7.6 solubility water: 2 tablet/liter, clear, colorless Featured Industry Diagnostic Assay Manufacturing
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Tris Buffered Saline T5912-1L
MDL number: MFCD00132476 NACRES: NA.25 ApplicationTris Buffered Saline has been used as washing buffer for electrodes. It was used to wash microtiter plates for western blot experiments and screening methods.Other NotesWashing buffer for alkaline phosphatase or peroxidase conjugates in Western blotting. Working solution will contain 20 mM Tris, pH approx. 7.4, and 0.9% NaClSuitabilitySuitable for use as a washing buffer for alkaline phosphatase or peroxidase conjugates in dot blot assay.Параметрыsterility 0.2 μm filtered Quality Level 200, form solution concentration 10 x storage temp. 2-8°C
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Tris buffered saline with Tween® 20
Кат. номер 91414-10TAB 91414-100TAB ApplicationTrizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris buffered saline with Tween 20, pH 7.6 is used as a washing buffer in assays such as enzyme-linked immunosorbent assays.Reconstitution1 tablet dissolved in 500 mL of deionized water yields:
0.15 M sodium chloride, 0.050 m TRIS-HCl buffer; 0..05% Tween® 20;
pH 7.6 at 25°CLegal InformationTWEEN is a registered trademark of Croda International PLCПараметрыproduct line BioUltra Quality Level 100, form tablet (for 500 mL) pH 7.6 solubility water: 2 tablet/liter, clear, colorless Featured Industry Diagnostic Assay Manufacturing
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Tris Buffered Saline, pH 8.0, with 3% nonfat Milk
NACRES: NA.25
Кат. номер T8793-10PAK T8793-5X10PAK T8793-25EA-KC T8793-25EA T8793-12EA-KC T8793-12EA PackagingFoil pouchesReconstitutionContents of one pouch, when dissolved in 100 mL of distilled or deionized water, will yield 0.05 M Tris buffered saline, pH 8.0 at 25°C, containing 3% (w/v) nonfat milk.Параметрыform powder Quality Level 200, Featured Industry Diagnostic Assay Manufacturing
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Tris Buffered Saline, with BSA, pH 8.0
Кат. номер T6789-5X10PAK T6789-10PAK Packaging10 pkg in foil bagReconstitutionContents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.05 M Tris buffered saline (NaCl - 0.138 M; KCl - 0.0027 M); bovine serum albumin - 1% (w/v), pH 8.0 at 25 °C.Параметрыform powder Quality Level 200, packaging 10 pak of (in foil bag) Featured Industry Diagnostic Assay Manufacturing storage temp. 2-8°C
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Tris Buffered Saline, with Tween® 20, pH 8.0
Кат. номер T9039-10PAK T9039-1PAK-KC T9039-1PAK T9039-5X10PAK ApplicationTris Buffered Saline, with Tween® 20, pH 8.0 has been used as a wash buffer in western blotting and enzyme linked immunosorbent assay. It has also been used along with 5% milk to block proteins on nitrocellulose in western blotting.Biochem/physiol ActionsTris Buffered Saline with Tween 20 is predominantly used as a wash buffer and also in blocking steps of immunoblotting technique.PackagingFoil pouchesReconstitutionContents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.05 M Tris buffered saline (NaCl - 0.138 M; KCl - 0.0027 M); TWEEN® 20 - 0.05%, pH 8.0, at 25 °C.Legal InformationTWEEN is a registered trademark of Croda International PLCПараметрыform powder Quality Level 200,
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TRIS Glycine buffer solution
Кат. номер 93321-1L 93321-VAR ApplicationTrizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. TRIS Glycine buffer solution, 10X concentrate is diluted to a working concentration of 1X and pH adjusted as required. TRIS Glycine buffer (TGB) is frequently used in gel electrophoresis and ion-exchange chromatography applications.Other NotesElectrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: Procedure and some applicationsПараметрыproduct line BioUltra Quality Level 100, form liquid quality 10x concentrate composition glycine, 1.92 M , TRIS, 0.25 M impurities insoluble matter, passes filter test, proteases, none detected ign. residue 0.2% pH 8.5-8.7 anion traces sulfate (SO42-): 50 mg/kg cation traces Al: 5 mg/kg, As: 0.1 mg/kg, Ba: 5 mg/kg, Bi: 5 mg/kg, Ca: 10 mg/kg, Cd: 5 mg/kg, Co: 5 mg/kg, Cr: 5 mg/kg, Cu: 5 mg/kg, Fe: 5 mg/kg, K: 50 mg/kg, Li: 5 mg/kg, Mg: 5 mg/kg, Mn: 5 mg/kg, Mo: 5 mg/kg, NH4+: 200 mg/kg, Na: 500 mg/kg, Ni: 5 mg/kg, Pb: 5 mg/kg, Sr: 5 mg/kg, Zn: 5 mg/kg neat UV absorption : 260 nm Amax: 0.1, : 280 nm Amax: 0.1
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Tris hydrochloride 10812846001
Empirical Formula (Hill Notation): C4H11NO3 · HCl Linear Formula: NH2C(CH2OH)3 · HCl CAS Number: 1185-53-1 Molecular Weight: 157.60 Beilstein/REAXYS Number: 3675235 MDL number: MFCD00012590 PubChem Substance ID: 329749168 ApplicationThe pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Buffering agent in incubation mixtures. It has also been used as a component of lysis and TE (Tris-EDTA) buffer.Features and BenefitsCrystalsQualityPurity: >99% Tris-HCl (titrimetric)
Heavy metals: <1 ppm Pb and FeOther NotesFor life science research only. Not for use in diagnostic procedures.Legal InformationTrizma is a registered trademark of Sigma-Aldrich Co. LLCПараметрыassay >99% (titration) form solid packaging pkg of 500 g Торговая марка Roche pH range 7.0 - 9.0 useful pH range 7.0 - 9.0 pKa (25 °C) 8.1 absorption <0.02 at 300 nm at 100 mg/mL shipped in ambient storage temp. room temp SMILES string Cl.NC(CO)(CO)CO InChI 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H InChI key QKNYBSVHEMOAJP-UHFFFAOYSA-N
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Tris(hydroxymethyl)aminomethane
Empirical Formula (Hill Notation): C4H11NO3 Linear Formula: NH2C(CH2OH)3 CAS Number: 77-86-1 Molecular Weight: 121.14 Beilstein/REAXYS Number: 741883 EC Number: 201-064-4 MDL number: MFCD00004679 PubChem Substance ID: 24855244 NACRES: NA.25
Кат. номер 252859-VAR 252859-500G 252859-100G General descriptionTris(hydroxymethyl)aminomethane is mainly required for preparation of buffers at physiological range of 7.3 to 7.5. The prepared buffers are compatible with biological fluids. It is of importance to laboratories as a standard pH solution.ApplicationTris(hydroxymethyl)aminomethane has been used as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.Packaging100, 500 g in poly bottleOther NotesEasily compare specifications for Trizma products with the Trizma specification table.ПараметрыQuality Level 200 grade ACS reagent assay ≥99.8%, 99.8-100.1% form crystalline impurities ≤0.005% insolubles, ≤2% water useful pH range 7 - 9 pKa (25 °C) 8.1 bp 219-220 °C/10 mmHg (lit.) mp 167-172 °C (lit.) cation traces Fe: ≤5 ppm, heavy metals (as Pb): ≤5 ppm absorption passes test SMILES string NC(CO)(CO)CO InChI 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2 InChI key LENZDBCJOHFCAS-UHFFFAOYSA-N
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Tris-Borate EDTA Buffer, 10X Concentrate
NACRES: NA.25
Кат. номер SRE0062-4L SRE0062-PH SRE0062-100ML SRE0062-1L ApplicationTris-Borate EDTA (TBE) buffer is commonly used in the electrophoresis of nucleic acids in agarose and polyacrylamide gels.
TBE buffer is recommended for resolution of RNA and DNA fragments smaller than 1500 bp.Physical form10X TBE buffer stock solution contains 0.89 M Trizma(R) with 0.02 M EDTA adjusted to pH 8.3 with boric acid. Dilute with ultrapure water to 1X.
Notes: If precipitation is present in the 10X concentrated buffer, warm the bottle to 37 °C and mix until completely dissolved prior to dilution.
It is recommended 1X working solutions be filtered through a 0.2 mm filter before use.ПараметрыQuality Level 500, form liquid Featured Industry Diagnostic Assay Manufacturing storage temp. room temp
Safety Informationpictograms GHS08 signalword Danger hcodes H360FD pcodes P201 - P202 - P280 - P308 + P313 - P405 - P501 RIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Tris-Borate-EDTA buffer
MDL number: MFCD00236358 PubChem Substance ID: 329826793 NACRES: NA.25
Кат. номер T4415-100ML-PW T4415-10L-PW T4415-1L-PW T3038-1L-PW T4415-10L T4415-20L T4415-PH T4415-1L T4415-4L T4415-100ML T4415-200L General descriptionTBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).ApplicationReady for use in gel electrophoresis after dilution to working concentrations.
Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE).
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1x TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5x or 10x stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.PackagingThe 4L, 10L and 20L sizes are supplied in dispenser with a spigot.Preparation NotePrepared with 18 megohm water
Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).Other NotesTBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.ПараметрыQuality Level 200, product line BioReagent impurities DNase and RNase, none detected suitability suitable for electrophoresis SMILES string OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O InChI 1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H InChI key OSBLTNPMIGYQGY-UHFFFAOYSA-N
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Tris-EDTA buffer solution
MDL number: MFCD00236359 eCl@ss: 32129211 PubChem Substance ID: 329770217 NACRES: NA.25
Кат. номер 93283-100ML 93283-VAR 93283-500ML ApplicationTrizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer.Other NotesThis buffer is 10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0.ПараметрыQuality Level 200 grade for molecular biology product line BioUltra form liquid impurities DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected pH 8.0±0.2 (25 °C) cation traces Al: 1 mg/kg, Ba: 1 mg/kg, Bi: 1 mg/kg, Ca: 5 mg/kg, Cd: 1 mg/kg, Co: 1 mg/kg, Cr: 1 mg/kg, Cu: 1 mg/kg, Fe: 1 mg/kg, K: 20 mg/kg, Li: 1 mg/kg, Mg: 1 mg/kg, Mn: 1 mg/kg, Mo: 1 mg/kg, Ni: 1 mg/kg, Pb: 1 mg/kg, Sr: 1 mg/kg, Zn: 1 mg/kg neat UV absorption : 260 nm Amax: 0.04, : 280 nm Amax: 0.01 SMILES string NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O InChI 1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2 InChI key VLEIUWBSEKKKFX-UHFFFAOYSA-N
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Tris-Glycine-SDS Buffer 10 Concentrate
eCl@ss: 32129211 NACRES: NA.25
Кат. номер T7200-96X1UG-PW T7777-PH T7777-1L General descriptionTris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both anode and cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.ApplicationTris-glycine-SDS buffer 10x concentrate has been used as a running buffer in sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE). It is also used to block membranes with 5% non-fat dry milk for western blotting. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.ReconstitutionDilution of the 10x TGS buffer produces a 1x running buffer containing 25 mM Tris, 192 mM glycine and 0.1% SDS, pH approx. 8.6.Параметрыsterility 0.2 μm filtered Quality Level 200, Featured Industry Diagnostic Assay Manufacturing storage temp. 2-8°C
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Tris-Tricine-SDS Buffer 10 Concentrate
NACRES: NA.25
Кат. номер T1165-500ML-PW T1165-PH T1165-500ML SRE0035-250ML-PW General descriptionTris-Tricine-SDS (TTS) running buffer is the cathode (upper reservoir) buffer for SDS−polyacrylamide gel electrophoresis of proteins using the Schagger and von Jagow method. The Schagger and von Jagow method is designed for the separation of small molecular weight proteins. It differs from the Laemmli method in that the glycine is replaced with tricine and the gel contains 1M Tris-HCl, pH 8.45 instead of 0.375 M Tris-HCl, pH 8.9. Dilution of the 10X TTS buffer produces a 1X cathode buffer containing 100 mM Tris, 100 mM tricine and 0.1% SDS. Recommended running conditions is 125 volts.ApplicationTris-Tricine-SDS Buffer 10x Concentrate is suitable for use as a running buffer for polyacrylamide based separation of bronchoalveolar lavage fluid (BALF).Other Notes1M Tris, 1M Tricine, 1% (w/v) SDS, pH approx. 8.2.Analysis NoteTested for use as a cathode buffer for Tris-Tricine gel electrophoresis to separate low molecular weight proteins.Параметрыsterility 0.2 μm filtered Quality Level 200, form liquid pH 8.05-8.30 (25 °C in water, high purity, 1:10) Featured Industry Diagnostic Assay Manufacturing storage temp. 2-8°C
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Trisodium citrate dihydrate
Empirical Formula (Hill Notation): C6H5Na3O7 · 2H2O Linear Formula: HOC(COONa)(CH2COONa)2 · 2H2O CAS Number: 6132-04-3 Molecular Weight: 294.10 Beilstein/REAXYS Number: 6104939 EC Number: 200-675-3 MDL number: MFCD00150031 PubChem Substance ID: 24899497 NACRES: NA.21
Кат. номер S1804-VAR S1804-1KG S1804-5KG S1804-500G S1804-25KG Packaging500 g in poly bottle
1 kg in poly bottle
5 kg in poly drumBiochem/physiol ActionsCitrate is a intermediate in the TCA cycle and fatty acid synthesis. Citrate is an allosteric modulator of acetyl-CoA carboxylase, the enzyme that regulates the conversion of acetyl-CoA to malonyl-CoA.ПараметрыAgency/Method meets USP testing specifications Quality Level 300, assay 99.0-100.5% anhydrous basis form powder pH 7.5-9 (25 °C, 29.4 g/L) mp >300 °C (lit.) Featured Industry Pharmaceutical (small molecule) SMILES string O.O.[Na+].[Na+].[Na+].OC(CC([O-])=O)(CC([O-])=O)C([O-])=O InChI 1S/C6H8O7.3Na.2H2O/c7-3(8)1-6(13,5(11)12)2-4(9)10;;;;;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);;;;2*1H2/q;3*+1;;/p-3 InChI key NLJMYIDDQXHKNR-UHFFFAOYSA-K
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Trizma® acetate
Empirical Formula (Hill Notation): C4H11NO3 · CH3COOH Linear Formula: NH2C(CH2OH)3 · CH3COOH CAS Number: 6850-28-8 Molecular Weight: 181.19 Beilstein/REAXYS Number: 3702918 EC Number: 229-939-6 MDL number: MFCD00038951 PubChem Substance ID: 24899948 NACRES: NA.25
Кат. номер T1258-25G T1258-EW T1258-100G T1258-250G Packaging100, 250 g in poly bottleLegal InformationTrizma is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200, assay 99.0% (titration) form crystalline powder pH 6.0-7.0 (0.1 M in water, high purity) solubility water: 0.5 M, clear, colorless SMILES string CC(O)=O.NC(CO)(CO)CO InChI 1S/C4H11NO3.C2H4O2/c5-4(1-6,2-7)3-8;1-2(3)4/h6-8H,1-3,5H2;1H3,(H,3,4) InChI key PIEPQKCYPFFYMG-UHFFFAOYSA-N
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Trizma® base
Empirical Formula (Hill Notation): C4H11NO3 Linear Formula: NH2C(CH2OH)3 CAS Number: 77-86-1 Molecular Weight: 121.14 Beilstein/REAXYS Number: 741883 EC Number: 201-064-4 MDL number: MFCD00004679 PubChem Substance ID: 24899973 NACRES: NA.25
Кат. номер T1503-25KG-DP T1503-384X500G T1503-48X5KG T1503-10KG T1503-1KG T1503-250G T1503-36.5KG T1503-25KG T1503-500G T1503-50KG T1503-5KG T1503-100G T1503-162X1KG T1503-25G General descriptionTris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.
40% (w/w) solution in water is clear and colorless.ApplicationTrizma® base has been used:- as a component of H buffer (cell dissociation buffer)
- for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique
- as an assay buffer for reconstitution of extracted and dried protein samples
- to prepare Tris-HCl buffer that is used to stabilize proteins
- as a buffer to extract carotenoid from tubers
- as a component of sample buffer during protein extraction prior to western blotting
- as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
- to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay
- as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate
Packaging5, 10, 25, 50 kg in poly drum
25, 100, 250, 500 g in poly bottle
1 kg in poly bottleOther NotesThe pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Easily compare specifications for Trizma products with the Trizma specification table.Legal InformationTrizma is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 300, 400 description aminopeptidase substrate assay 99.9% (titration) form crystalline pH 10.5-12 useful pH range 7 - 9 pKa (25 °C) 8.1 bp 219-220 °C/10 mmHg (lit.) mp 167-172 °C (lit.) solubility water: soluble (678 g/l at 20 °C) absorption 0.05 at 290 nm at 40% Featured Industry Diagnostic Assay Manufacturing SMILES string NC(CO)(CO)CO InChI 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2 InChI key LENZDBCJOHFCAS-UHFFFAOYSA-N
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Trizma® base solution T1699-100ML
CAS Number: 77-86-1 PubChem Substance ID: 24899986 NACRES: NA.25 ApplicationFor use in the enzymatic determination of pyruvate using lactic dehydrogenase.CautionContains 0.05% sodium azide as a preservative.Legal InformationTrizma is a registered trademark of Sigma-Aldrich Co. LLCПараметрыform liquid Quality Level 200, concentration 1.5 M pH 10.7-11.1 Featured Industry Diagnostic Assay Manufacturing storage temp. 2-8°C InChI 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2 InChI key LENZDBCJOHFCAS-UHFFFAOYSA-N
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Trizma® Buffer substance
MDL number: MFCD00012590 PubChem Substance ID: 57653542
Кат. номер 93367-500G 93367-2.5KG Applicationwater stabilizer for use in aquariums and for transporting tropical fish; stabilizes pH value and reduces CO2-level; suitable for marine speciesLegal InformationTrizma is a registered trademark of Sigma-Aldrich Co. LLCПараметрыgrade for fish-farming form powder or crystals mol wt average Mw 135.4 pH 8.30±0.05 (25 °C, 0.05 m in H2O) solubility H2O: 0.05 M at 20 °C, clear, faintly yellow SMILES string Cl.NC(CO)(CO)CO InChI 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H InChI key QKNYBSVHEMOAJP-UHFFFAOYSA-N
Safety Informationpictograms GHS07 signalword Warning hcodes H315-H319-H335 pcodes P261-P305 + P351 + P338 Personal Protective Equipment dust mask type N95 (US),, Eyeshields,, Gloves, RIDADR NONH for all modes of transport WGK Germany 2 Узнать цену -
Trizma® citrate dibasic solution
Empirical Formula (Hill Notation): C8H22N2O6 · C6H8O7 Linear Formula: (C4H11NO3)2 · C6H8O7 CAS Number: 108321-34-2 Molecular Weight: 434.39 MDL number: MFCD00058331 PubChem Substance ID: 329826725 NACRES: NA.25
Кат. номер T2785-250ML T2785-50ML T2785-500ML Legal InformationTrizma is a registered trademark of Sigma-Aldrich Co. LLCПараметрыform solution Quality Level 200, concentration 1 M pH 5.0-5.2 (0.05 M) SMILES string NC(CO)(CO)CO.NC(CO)(CO)CO.OC(=O)CC(O)(CC(O)=O)C(O)=O InChI 1S/C6H8O7.2C4H11NO3/c7-3(8)1-6(13,5(11)12)2-4(9)10;2*5-4(1-6,2-7)3-8/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);2*6-8H,1-3,5H2 InChI key XDJZTRQRZFHVTE-UHFFFAOYSA-N
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Trizma® citrate tribasic solution
Empirical Formula (Hill Notation): C12H33N3O9 · C6H8O7 Linear Formula: (C4H11NO3)3 . C6H8O7 CAS Number: 6986-91-0 Molecular Weight: 555.53 MDL number: MFCD00058332 PubChem Substance ID: 329826722 NACRES: NA.25
Кат. номер T2660-250ML-PW T2660-250ML T2660-50ML T2660-500ML Legal InformationTrizma is a registered trademark of Sigma-Aldrich Co. LLCПараметрыform liquid Quality Level 200, concentration 1 M SMILES string NC(CO)(CO)CO.NC(CO)(CO)CO.NC(CO)(CO)CO.OC(=O)CC(O)(CC(O)=O)C(O)=O InChI 1S/C6H8O7.3C4H11NO3/c7-3(8)1-6(13,5(11)12)2-4(9)10;3*5-4(1-6,2-7)3-8/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);3*6-8H,1-3,5H2 InChI key OHSJHRFFVBSWTL-UHFFFAOYSA-N
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Trizma® hemisulfate
Empirical Formula (Hill Notation): C4H11NO3 · 0.5H2SO4 Linear Formula: NH2C(CH2OH)3 · 0.5 H2SO4 CAS Number: 6992-38-7 Molecular Weight: 170.17 Beilstein/REAXYS Number: 3718745 EC Number: 230-259-7 MDL number: MFCD00039107 PubChem Substance ID: 24900523 NACRES: NA.25
Кат. номер T8379-25G T8379-250G T8379-100G Packaging100 g in poly bottleLegal InformationTrizma is a registered trademark of Sigma-Aldrich Co. LLCПараметрыassay 99% NaOH basis (titration) form powder solubility water: 0.5 g/mL, clear, colorless SMILES string OS(O)(=O)=O.NC(CO)(CO)CO.NC(CO)(CO)CO InChI 1S/2C4H11NO3.H2O4S/c2*5-4(1-6,2-7)3-8;1-5(2,3)4/h2*6-8H,1-3,5H2;(H2,1,2,3,4) InChI key OLJBMOUMSAPNML-UHFFFAOYSA-N
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany 3 Узнать цену -
Trizma® hydrochloride
Empirical Formula (Hill Notation): C4H11NO3 · HCl Linear Formula: NH2C(CH2OH)3 · HCl CAS Number: 1185-53-1 Molecular Weight: 157.60 Beilstein/REAXYS Number: 3675235 EC Number: 214-684-5 MDL number: MFCD00012590 PubChem Substance ID: 24900332 NACRES: NA.25
Кат. номер T5941-50KG T5941-100G-KC T5941-1KT T5941-5KG T5941-500G T5941-EW T5941-100G T5941-1KG T5941-6X500G T5941-BR T5941-500G-PB T5941-1KG-PB ApplicationThe pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.Packaging1 kg in poly bottle
100, 500 g in poly bottle
5 kg in poly drum
T5941-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.Other NotesEasily compare specifications for Trizma HCl products with the Trizma HCl specification table,.Legal InformationTrizma is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 400, grade BioPerformance Certified assay 99.0% (titration) form crystalline powder application(s) cell culture | mammalian: suitable, electrophoresis: suitable impurities DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected, endotoxin and total aerobic microbial count, tested, 0.5% water (Karl Fischer) useful pH range 7.0 - 9.0 pKa (25 °C) 8.1 mp 150-152 °C solubility H2O: 667 mg/mL absorption 0.05 at 290 at 40% suitability suitable for electrophoresis Featured Industry Diagnostic Assay Manufacturing SMILES string Cl.NC(CO)(CO)CO InChI 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H InChI key QKNYBSVHEMOAJP-UHFFFAOYSA-N
Safety InformationPersonal Protective Equipment dust mask type N95 (US),, Eyeshields,, Gloves, RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Trizma® hydrochloride buffer solution 41573-1L-F
Beilstein/REAXYS Number: 3675235 MDL number: MFCD00012590 PubChem Substance ID: 57650070 NACRES: NA.25 Legal InformationTrizma is a registered trademark of Sigma-Aldrich Co. LLCПараметрыgrade for molecular biology Quality Level 100, product line BioUltra form liquid impurities DNases, RNases, none detected, insoluble matter, passes filter test, proteases, phosphatases, none detected pH 7.2±0.1 (25) density 1.05 g/mL at 20 °C cation traces Al: 1 mg/kg, As: 0.1 mg/kg, Ba: 1 mg/kg, Bi: 1 mg/kg, Ca: 5 mg/kg, Cd: 1 mg/kg, Co: 1 mg/kg, Cr: 1 mg/kg, Cu: 1 mg/kg, Fe: 1 mg/kg, K: 20 mg/kg, Li: 1 mg/kg, Mg: 1 mg/kg, Mn: 1 mg/kg, Mo: 1 mg/kg, Na: 20 mg/kg, Ni: 1 mg/kg, Pb: 1 mg/kg, Sr: 1 mg/kg, Zn: 1 mg/kg neat UV absorption : 260 nm Amax: 0.03, : 280 nm Amax: 0.04 Featured Industry Diagnostic Assay Manufacturing SMILES string Cl.NC(CO)(CO)CO InChI 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H InChI key QKNYBSVHEMOAJP-UHFFFAOYSA-N
Safety Informationpictograms GHS05 signalword Warning hcodes H290 pcodes P234 - P390 Personal Protective Equipment Faceshields,, Gloves,, Goggles,, type ABEK (EN14387) respirator filter, RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Trizma® hydrochloride solution
NACRES: NA.25
Кат. номер T2319-1L-PW T2444-100ML-PW T2694-PH T2694-100ML T2694-1L T2694-500ML General descriptionA series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.ApplicationTrizma® hydrochloride solution has been used:- as a component of extraction buffer for Plasmodiophora brassicae DNA isolation
- as a component of resuspension buffer for human genomic DNA fragmented sample
- for adjusting pH of plasma samples and as a component of reconstitution and neutralization buffer
Legal InformationTrizma is a registered trademark of Sigma-Aldrich Co. LLCПараметрыgrade BioPerformance Certified, for molecular biology Quality Level 200, form solution concentration 1 M application(s) cell culture | mammalian: suitable impurities DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, 5 ppm Heavy metals (as Pb) pH 8.0 useful pH range 7.0 - 9.0 absorption 0.05 at 290 at 40%
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type ABEK (EN14387) respirator filter, RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену
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