Tris Acetate-EDTA buffer

MDL number:	MFCD00236357
PubChem Substance ID:	329826908
NACRES:	NA.25

Кат. номер

T7688-20ML-PW

T9650-4L-PW

T7688-100ML-PW

T9650-PH

T9650-1L-PW

T9650-4L

T9650-1L

T9650-6X1L

Tris Acetate-EDTA buffer has been used for the preparation of agarose gel during DNA agarose gel electrophoresis.
TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1x TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1x TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
0.4 M Tris acetate, pH approx. 8.3, containing 0.01 M EDTA.
Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).
Solution prepared with 18 megohm water

Quality Level	200,
grade	for molecular biology
sterility	non-sterile; 0.2 μm filtered
product line	BioReagent
form	solution
suitability	suitable for gel electrophoresis (after dilution to working concentration)
SMILES string	CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI	1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
InChI key	HGEVZDLYZYVYHD-UHFFFAOYSA-N

pictograms	GHS08
signalword	Warning
hcodes	H373
pcodes	P260 - P313 - P501
Target Organs	Respiratory Tract
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable