Biochemicals
- Сортировать:
- Вид таблицей
-
Lysosome Isolation Kit MAK405-1KT
General descriptionLysosomes are membrane-bound cell organelles found in most animal cells (except red blood cells). Lysosomes are spherical vesicles containing hydrolytic enzymes capable of breaking down different kinds of biomolecules, including proteins, nucleic acids, carbohydrates, lipids, and cellular debris. They are known to contain over 50 different enzymes, which are active under acidic conditions. Thus, lysosomes act as the waste disposal system of the cells by digesting unwanted materials in the cytoplasm.Features and BenefitsLysosome Isolation Kit:- Provides unique formulations of ready-to-use buffers and reagents to isolate lysosomes with minimal sample preparation.
SuitabilityThe kit is suitable for the isolation of high purity lysosomes from mammalian tissues (liver, kidney, etc.) and cultured cells (suspension and adherent cells).PrincipleThe Lysosome Isolation Kit provides a procedure for isolating enriched or purified lysosomal fraction from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation.ПараметрыQuality Level 100, storage temp. −20°C Узнать цену -
Lysozyme Inhibitor Screening Kit MAK393-1KT
General descriptionLysozyme, also known as muramidase or N-acetylmuramide glycanhydrolase, is a glycoside hydrolase. It is ubiquitously found in a wide range of biological fluids such as tears, saliva, serum, where it serves as a key effector of the innate immune system. It is also synthesized by certain carcinomas. The excessive production of lysozymes by cancer cells (especially myelomonocytic leukemia) results in higher levels of lysozyme causing renal failure. It has been demonstrated that the expression level of lysozyme is an independent prognostic factor which can be used to predict both relapse-free survival and overall survival in patients with breast carcinomas. Therefore, lysozyme inhibitors may constitute attractive, potential targets for developing anti-inflammatory and/or anti-tumor drugs.Features and BenefitsLysozyme Inhibitor Screening Kit:- Sensitive and stable.
- Compatible with high-throughput handling systems.
SuitabilityThe kit is suitable for the screening, studying and characterizing of lysozyme inhibitors.PrincipleThe Lysozyme Inhibitor Screening Kit uses N,N,N-Triacetylchitotriose, a competitive inhibitor that binds to the active site of lysozyme. The inhibitor decreases the catalytic ability of lysozyme to hydrolyze a fluorogenic substrate.ПараметрыQuality Level 100, detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H334 pcodes P261 - P284 - P304 + P340 + P312 - P501 Узнать цену -
Magnesium Assay Kit MAK026-1KT
NACRES: NA.84 General descriptionMagnesium, one of the most abundant trace metals in living organisms, contributes to a variety of biological functions including ATP and nucleic acid processing, energy metabolism, and enzymatic function. Magnesium forms complexes with multiple molecules such as phospholipids and ATP. Low serum levels of magnesium have been associated with metabolic syndrome, diabetes mellitus type 2, and hypertension, as well as, increased smooth muscle and platelet reactivity.ApplicationMagnesium Assay Kit has been used to quantify magnesium levels in conditioned media.SuitabilitySuitable for quantitating magnesium concentrations in a variety of biological samples.PrincipleThe Magnesium Assay kit provides a simple and direct procedure for measuring magnesium in a variety of samples. The magnesium concentration is determined by a coupled enzyme assay that takes advantage of the specific requirement of glycerol kinase for Mg2+, resulting in a colorimetric (450 nm) product proportional to the magnesium present. This assay gives a linear range of 3-15 nmoles of magnesium and exhibits no detectable interference with Fe2+, Cu2+, Ni2+, Zn2+, Co2+, Ca2+, and Mn2+.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05,GHS08 signalword Danger hcodes H314 - H334 pcodes P261 - P280 - P284 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 RIDADR NONH for all modes of transport Узнать цену -
Malachite Green Phosphate Assay Kit MAK307-1KT
NACRES: NA.84 General descriptionPhosphate is one of the most abundant minerals in the human body and contains about 1 % of total body weight. Phosphate homeostasis is maintained by the several regulators such as parathyroid hormone (PTH), calcitriol and phosphatonins. Maintenance of phosphate homeostasis is essential for various biological processes, such as energy metabolism, cell signaling, regulation of protein synthesis, skeletal development and bone integrity.† An adequate level of phosphate is needed for the process of apoptosis of mature chondrocytes in the growth plate. Phosphate deficiency is associated with the bone pathology and clinical illness.†ApplicationMalachite Green Phosphate Assay Kit has been used in phosphate quantification and steady-state kinetics.
Malachite Green Phosphate Assay Kit has been used to measure the liberation of free orthophosphate in the following applications:- Phosphatase Assays: liberation of phosphate from peptide, protein, or small molecule substrate.
- Lipase Assays: liberation of phosphate from phospholipids
- Nucleoside Triphosphatase Assays: liberation of phosphate from nucleoside triphosphates (ATP, GTP, TTP, CTP etc).
- Quantitation of phosphate in phospholipids, proteins and DNAs, etc.
- Drug Discovery: high-throughput screen for phosphatase inhibitors.
Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for phosphate determination in a variety of biological and environmental samples.PrincipleMalachite Green is a basic dye belonging to the triphenylmethane group. It is used in biology for the staining of microscopic samples, such as plant tissue, yeast and bacteria. The malachite green method is a sensitive method for the detection of released phosphate.†† The Malachite Green Phosphate Assay Kit is based on quantification of the green complex formed between Malachite Green, molybdate and free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600 - 660 nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be executed in tubes, cuvettes or multi-well plates. The assays can be conveniently performed in 96 and 384 well plates for high throughput screening of enzyme inhibitors.Параметрыusage sufficient for 2,500 colorimetric tests detection method colorimetric storage temp. 2-8°C
Safety InformationPictograms GHS05 Signal Word Warning Hazard Statements H290 - H315 - H319 - H412 Precautionary Statements P305 + P351 + P338 RIDADR UN 2796 8 / PGII Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Malate Assay Kit MAK067-1KT
NACRES: NA.84 General descriptionL-Malate is a tricarboxylic acid cycle intermediate and a critical component of the malate-aspartate shuttle. The Malate-aspartate shuttle is required for transporting reducing equivalents across the mitochondrial membrane for use in oxidative phosphorylation. In lower organisms, malate is converted to lactate during malolactic fermentation with the formation of CO2. Malate is frequently used as an additive in the food and pharmaceutical industries, so quantitating malate is important in the manufacturing of beer, wine, cheese, and fruits. The Malate Assay kit is an easy and sensitive assay to measure the malate level in a variety of samples.
In this kit, malate is oxidized to generate a product specifically detected by colorimetric (450 nm) assay. Typical sensitivities of detection for this kit are 1-35 nmol of malate.ApplicationMalate Assay Kit has been used to determine the concentration of malate in samples.SuitabilitySuitable for the measurement of L(-) Malate level in a variety of samples including tissue, cells and food and beverages (for example beer, wine, cheese and fruit)PrincipleMalate concentration is determined by an enzymatic assay, which results in a colorimetric (450 nm) product, proportional to the malate present. Typical detection range for this kit is 5–25 nmoles of malate.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Malate Dehydrogenase Assay Kit MAK196-1KT
NACRES: NA.84 General descriptionMalate dehydrogenase (MDH; EC 1.1.1.37) is an NAD oxidoreductase that catalyzes the NAD/NADH-dependent interconversion of malate and oxalacetate. This reaction is critical to maintain the malate/aspartate shuttle across the mitochondrial membrane and Krebs tricarboxylic acid cycle in the mitochondrial matrix. Increased MDH activity has been observed in neurodegenerative diseases such as Alzheimer′s disease.ApplicationMalate Dehydrogenase Assay Kit has been used in LDH (lactate dehydrogenase) and malate dehydrogenase (MDH1) assays. It has also been used to estimate isocitrate dehydrogenase and succinate dehydrogenase activities.SuitabilitySuitable for the detection of Malate Dehydrogenase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.PrincipleThe Malate Dehydrogenase Assay Kit provides a simple and sensitive procedure for measuring MDH activity in a variety of tissues, cell cultures, and isolated mitochondria. MDH activity is determined by generating a product with absorbance at 450 nm proportional to the enzymatic activity present. One unit of MDH is the amount of enzyme that generates 1.0 μmole of NADH per minute at 37 °C and pH 9.5.Параметрыdetection method colorimetric storage temp. 20°C Gene Information human ... MDH1(4190), MDH1B(130752), MDH2(4191)
mouse ... MDH1(17449), MDH1B(76668), MDH2(17448)
rat ... MDH1(24551), MDH1B(316444), MDH2(81829)
Safety Informationpictograms GHS07 signalword Warning hcodes H315 - H319 - H335 pcodes P261 - P305 + P351 + P338 Target Organs Respiratory system RIDADR NONH for all modes of transport Узнать цену -
Maltose and Glucose Assay Kit MAK018-1KT
General descriptionMaltose is a disaccharide containing two glucose molecules with an a(1→4) glycosidic linkage. The enzyme α-D-glucosidase hydrolyzes maltose to its constituent monosaccharides. Glucose is the primary carbohydrate utilized for energy during cellular respiration. Maltose can be derived from starch in food through the action of amylase. Maltose can be found in many food products, including beer, cereals, and pasta.ApplicationMaltose and Glucose Assay Kit has been used to estimate maltose present in coral, coral larvae, Symbiodinium and seawater.SuitabilitySuitable for the direct detection of maltose and glucose in a variety of samples, including serum, plasma, food, or growth media.PrincipleThe Maltose and Glucose Assay kit provides a simple and direct procedure for measuring maltose and glucose in a variety of samples, including serum, plasma, food, or growth media. In this kit, maltose is converted to two glucose units via -D-Glucosidase. Glucose is further oxidized, resulting in a colorimetric (570 nm)/fluorometric (ex = 535/em = 587 nm) product, proportional to the maltose present. This kit has a linear detection range of 0.1-0.5 nmole maltose for the fluorometric assay and 1-5 nmoles maltose for the colorimetric assay.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. 20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H334 - H412 pcodes P261 - P273 - P284 - P501 Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Maltose Assay Kit MAK019-1KT
NACRES: NA.84 General descriptionMaltose is a disaccharide containing two glucose molecules with an a(1→4) glycosidic linkage. Maltose can be derived from starch in food through the action of amylase. Maltose can be found in many food products, including beer, cereals, and pasta.SuitabilitySuitable for the direct detection of maltose in a variety of samples, including serum, plasma, food, or growth media.PrincipleThe Maltose Assay kit provides a simple and direct procedure for measuring maltose in a variety of samples, including serum, plasma, food, or growth media. In this kit, maltose is converted to two glucose units via -D-Glucosidase. Glucose is further oxidized, resulting in a colorimetric (570 nm)/fluorometric (ex = 535/em = 587 nm) product, proportional to the maltose present.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H334 - H412 pcodes P261 - P273 - P284 - P501 Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Mannosidase Assay Kit MAK318-1KT
NACRES: NA.84 General descriptionα-Mannosidase (AMA) is an enzyme which catalyzes the cleavage of the alpha form of mannose. α-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes α-mannosidosis and leads to deterioration of the central nervous system in children.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for determining α-Mannosidase (AMA) activity in a variety of biological samples such as plasma, serum, tissue, and cell culture media.PrincipleThe -Mannosidase Activity Assay Kit provides a simple and high-throughput adaptable assay for determining -Mannosidase activity in a variety of biological samples such as plasma, serum, tissue, and cell culture media. In this assay 4-nitrophenol is cleaved from a synthetic substrate. Nitrophenol becomes intensely colored with the addition of the stop reagent, producing a colorimetric (405 nm) result, directly proportional to the enzyme activity in the sample.
One unit of AMA is the amount of enzyme that will convert 1.0 µmole of 4-Nitrophenyl--D-mannopyranoside to 4-Nitrophenol and -D-Mannose per minute at 25 °C and pH 4.5. The kit has a linear detection range of 1–250 unit/L for a 10 minute reaction in a 96 well format.Параметрыdetection method colorimetric storage temp. 2-8°C
Safety Informationpictograms GHS07 signalword Warning hcodes H319 pcodes P264 - P280 - P305 + P351 + P338 - P337 + P313 RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Methanol Assay Kit MAK353-1KT
General descriptionMethanol is the simplest alcohol, consisting exclusively of a methyl group and a hydroxyl moiety. It is both an important industrial molecule (solvent, fuel, building block for chemical synthesis) and a biological metabolite; many bacteria generate methanol as a result of anaerobic metabolism. In humans, ingestion of large quantities of methanol is toxic and suppresses the nervous system; this is termed ‘methanol poisoning. As such, methanol is frequently used as an additive in industrial alcohols to prevent human consumption. The toxicity of methanol is primarily due to the biological product of its metabolism, formaldehyde, which is further metabolized into formic acid. At lower concentrations, methanol poisoning can cause loss of coordination and discomfort, and at higher concentrations will lead to kidney failure, blindness, and even death. In the gut, microbial breakdown of pectin-rich foods produces small amounts of methanol. In addition, some bacteria are capable of metabolizing methane gas, generating methanol, and so monitoring methanol concentration is also relevant for industrial purposes and renewable energy research.SuitabilitySuitable for the determination of methanol concentration in biological samples (serum, plasma and urine), cell and tissue culture supernatants, fruit juice and other non-alcoholic beverages and anaerobic microbial cultures.PrincipleThe Methanol Assay Kit utilizes an enzymatic mechanism by which conversion of methanol is correlated stoichiometrically with generation of a colorimetric signal that can be quantified at 450 nm. The assay shows greater than 100-fold specificity for methanol over ethanol and can detect as little as 500 pmol methanol.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS02,GHS06,GHS08 signalword Danger hcodes H225 - H301 + H311 + H331 - H315 - H319 - H370 pcodes P210 - P280 - P301 + P310 + P330 - P302 + P352 + P312 - P304 + P340 + P311 - P305 + P351 + P338 Target Organs Eyes Flash Point F 49.5 °F Flash Point C 9.7 °C Узнать цену -
Methionine Adenosyltransferase Activity Assay Kit (Colorimetric) MAK431-1KT
General descriptionMethionine Adenosyltransferases (MATs), also known as Adenosylmethionine Synthetases are a family of enzymes that synthesize S-adenosyl-L-methionine (SAMe) from L-methionine and ATP. SAMe is a primary biochemical alkylation agent (one of only two possible methyl group donors) and is also a vital metabolic precursor of the trans-sulfuration and polyamine synthesis (aminopropylation) pathways. All organisms express at least one MAT enzyme. In mammals, three isozymes of MAT have been identified. MAT1 and MAT3 isozymes are predominantly expressed in the liver, whereas MAT2A is expressed in most tissues. Upregulation of the MAT2A isozyme has been linked to several human diseases. MAT2A has become a popular drug target for novel cancer therapeutics as well as for hepatic fibrosis and non-alcoholic fatty liver disease.Features and BenefitsMethionine Adenosyltransferase Activity Assay Kit:- Compatible with high-throughput handling systems.
- Limit of quantification of 2 mU MAT activity per well.
- Homogeneous assay.
- Does not require complicated sample processing.
SuitabilityThe kit is suitable for the rapid assessment of MAT activity in human or animal soft tissue (i.e. brain, liver, lung, etc.) homogenates and cultured cell lysates (adherent or suspension cells) or heterologously expressed recombinant MAT preparations.PrincipleThe Methionine Adenosyltransferase Activity Assay Kit enables the rapid measurement of MAT activity in complex biological matrices. The assay is based on the detection of pyrophosphate, which is generated stoichiometrically during the generation of SAMe. Pyrophosphate is enzymatically metabolized to an intermediate product, which reacts with the probe to form a stable chromophore that is detected by absorbance at A570 nm.ПараметрыQuality Level 100, detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 pcodes P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 Узнать цену -
Methionine Assay Kit (Fluorometric) MAK347-1KT
General descriptionMethionine (Met) is one of the twenty proteogenic amino acids encoded by the genetic code, and one of nine essential amino acids, meaning it cannot be synthesized in vivo and must be obtained from dietary sources. Met is one of two sulfur-containing amino acids (the other being cysteine) and can serve as a precursor in cysteine biosynthesis. S-adenosylmethionine, another product of Met metabolism, is an important co-factor and methyl donor in a number of biological reactions such as methylation and polyamine synthesis. Met is also an intermediate species in the synthesis of phospholipids such as lecithin, and other small precursor molecules including taurine, an aminosulfonic acid. Oxidation of Met generates methionine sulfoxide, a species whose concentration tends to increase with age and is known to cause protein mis-folding and loss-of-function of numerous enzymes. This version of the amino acid can be restored back to methionine by another enzyme, methionine sulfoxide reductase. Biologically, Met levels can affect important events such as angiogenesis, protein synthesis, and cartilage production.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the determination of methionine concentration in serum, plasma, cell cultures and tissue lysatesPrincipleThe Methionine Assay Kit utilizes an enzymatic mechanism by which metabolism of methionine is coupled with the stoichiometric generation of hydrogen peroxide. A probe generates fluorometric signal that can be quantified at Ex/Em: 535/587 nm. The assay shows greater than 10-fold specificity for methionine over cysteine and greater than 40-fold selectivity over other standard and non-standard amino acids. After utilization of a proprietary Sample Clean-Up Mix, the method is suitable for use in blood samples, and can detect concentrations as low as 0.5 μM, or 25 pmole Methionine.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H360D pcodes P201 - P202 - P280 - P308 + P313 - P405 - P501 Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
Microsome Isolation Kit MAK340-1KT
General descriptionMicrosomes are spherical vesicle-like structures formed from membrane fragments following homogenization and fractionation of eukaryotic cells. The microsomal subcellular fraction is prepared by differential centrifugation and consists primarily of membranes derived from the endoplasmic reticulum (ER) and Golgi apparatus. Microsomes isolated from liver tissue are used extensively in pharmaceutical development, toxicology and environmental science to study the metabolism of drugs, organic pollutants and other xenobiotic compounds by the cytochrome P450 monooxidase (CYP) enzyme superfamily. Microsomal preparations are an affordable and convenient in vitro system for assessing Phase I biotransformation reactions, as they contain all of the xenobiotic-metabolizing CYP isozymes and the membrane-bound flavoenzymes (such as NADPH P450-Reductase and cytochrome b5) required for function of the multicomponent P450 enzyme system.SuitabilitySuitable for the isolation of Microsomes from mammalian glands and soft tissues such as liver, spleen, lungs etc. or cultured eukaryotic cell lines such as HepG2 human hepatic carcinoma cellsPrincipleThe Microsome Isolation Kit enables preparation of active microsomes in about one hour, without the need for ultracentrifugation or sucrose gradient fractionation. The kit contains sufficient reagents for 50 isolation procedures, yielding microsomes from roughly 25 grams of tissue or cultured cells.Параметрыusage sufficient for 50 isolation(s) storage temp. −20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H319 pcodes P305 + P351 + P338 Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
Mitochondrial Complex I Activity Assay Kit MAK359-1KT
General descriptionMitochondrial Complex I or NADH:ubiquinone oxidoreductase is the first and the largest complex of the Electron Transport Chain located in the mitochondrial membrane. It oxidizes NADH to NAD+ and transfers an electron to ubiquinone (also present in the inner mitochondrial membrane) converting it to ubiquinol. During this process, it transports protons across the inner mitochondrial membrane, helping to develop an electrochemical gradient. This process is very important for cellular respiration and adverse effects on Complex I activity can compromise mitochondrial respiration, which further leads to cellular stress.SuitabilitySuitable for the measurement of Mitochondrial Complex I activity in isolated mitochondria.PrincipleThe Mitochondrial Complex I Assay Kit is a fast and reliable method to determine the activity of complex I in isolated mitochondria. It is useful for respiration studies in isolated mitochondria and may be used to study effects of toxicants, drugs and other environmental conditions on mitochondrial complex I activity. This kit uses decylubiquinone, an analog of ubiquinone, as an electron acceptor that gets converted to decylubiquinol through the catalytic activity of Complex I. The Complex I dye that absorbs light at A600 nm in its oxidized form is used as a terminal electron acceptor that accepts electrons from decylubiquinol. Complex I activity is determined colorimetrically by recording the change in absorbance of reduced Complex I dye at A600 nm. Specific Complex I activity is obtained by subtracting the activity in presence of Complex I inhibitor rotenone from total activity. This kit can detect as low as 0.1 mU / well and is linear up to 7 mU / well.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric
Safety Informationhcodes H412 pcodes P273 - P501 Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
Mitochondrial Complex III Activity Assay Kit MAK360-1KT
General descriptionMitochondrial Complex III or Ubiquinol-Cytochrome c reductase is the third complex of the Electron Transport Chain located in the mitochondrial membrane. It is present in the mitochondria of all aerobic eukaryotes as well as in the inner membranes of most bacteria. It transfers electrons from CoQH (reduced coenzyme Q or ubiquinol) to cytochrome c, resulting in the reduction of cytochrome c. This reduced cytochrome c is a substrate for complex IV of the electron transport chain.SuitabilitySuitable for the measurement of Mitochondrial Complex III activity in isolated mitochondria.PrincipleThe Mitochondrial Complex III Activity Assay is based on the reduction of cytochrome c through the activity of Complex III. The absorbance of reduced cytochrome c can be measured at A550 nm. It is a fast and reliable method to determine the activity of complex III in isolated mitochondria. The kit can detect as low as 10 mU/mL and is linear up to 30 mU/mL.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety InformationFlash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
Mitochondrial Membrane Potential Kit MAK160-1KT
NACRES: NA.25 General descriptionMitochondria generate a potential across their membranes due to the activities of enzymes of the electron transport chain. During apoptosis, collapse of the mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.ApplicationMitochondrial Membrane Potential Kit has been used to detect mitochondrial membrane potential (MMP) of PC-12 cells.SuitabilityThis kit is suitable for the flow cytometric detection of Mitochondrial Membrane Potential in mammalian cells and for screening apoptosis inhibitors and activators.PrincipleThis kit utilizes JC-10, a superior alternative to JC-1, for determining the loss of the MMP in cells. Although JC-1 is widely used in many labs, its poor water solubility often results in precipitation in aqueous buffers when used at higher concentrations. At higher concentrations, JC-10 exhibits greater aqueous solubility than JC-1. Similar to JC-1, JC-10 is a cationic, lipophilic dye that is concentrated and forms reversible red-fluorescent JC-10 aggregates (ex = 540/em = 590 nm) in the mitochondria of cells with a polarized mitochondrial membrane. In apoptotic cells, MMP collapse results in the failure to retain JC-10 in the mitochondria and a return of the dye to its monomeric, green fluorescent form (ex = 490/em = 525 nm). This kit can be used for monitoring apoptosis and for screening apoptosis inhibitors and activators.Параметрыusage sufficient for 100 fluorometric tests (flow cytometry) detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Mitochondrial Membrane Potential Kit MAK159-1KT
NACRES: NA.25 General descriptionMitochondria generate a potential across their membranes due to the activities of enzymes of the electron transport chain. During apoptosis, collapse of the mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.ApplicationMitochondrial Membrane Potential Kit has been used to measure mitochondrial membrane potential.SuitabilityThis kit is suitable for the detection of Mitochondrial Membrane Potential in mammalian cells and for screening apoptosis inhibitors and activators using microplate readers.PrincipleThis kit utilizes JC-10, a superior alternative to JC-1, for determining the loss of the MMP in cells. Although JC-1 is widely used in many labs, its poor water solubility often results in precipitation in aqueous buffers when used at higher concentrations. At higher concentrations, JC-10 exhibits greater aqueous solubility than JC-1. Similar to JC-1, JC-10 is a cationic, lipophilic dye that is concentrated and forms reversible red-fluorescent JC-10 aggregates (ex = 540/em = 590 nm) in the mitochondria of cells with a polarized mitochondrial membrane. In apoptotic cells, MMP collapse results in the failure to retain JC-10 in the mitochondria and a return of the dye to its monomeric, green fluorescent form (ex = 490/em = 525 nm). This kit can be used for monitoring apoptosis and for screening apoptosis inhibitors and activators.Параметрыusage sufficient for 500 fluorometric tests (microplate readers) detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Mitochondrion Membrane Potential Kit MAK146-1KT
NACRES: NA.25 General descriptionMitochondria generate a potential across their membranes due to the activities of enzymes of the electron transport chain. During apoptosis, collapse of the mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.SuitabilityThis kit is suitable for the flow cytometric detection of Mitochondrial Membrane Potential in mammalian cells and for screening apoptosis inhibitors and activators.PrincipleThis kit is optimized for the analysis of the MMP by flow cytometry on instruments with a 488 nm laser. The cationic hydrophobic mitochondrial potential dye accumulates in normal mitochondria, most likely due to the mitochondrial potential, resulting in an increase in fluorescence (em = 535 nm). In apoptotic cells, MMP collapse results in decreased fluorescence. This kit can be used for monitoring apoptosis and for screening apoptosis inhibitors and activators.
This kit is optimized for the analysis of the MMP by flow cytometry on instruments with a 488 nm laser. The cationic hydrophobic mitochondrial potential dye accumulates in normal mitochondria, most likely due to the mitochondrial potential, resulting in an increase in fluorescence (λem = 535 nm). In apoptotic cells, MMP collapse results in decreased fluorescence. This kit can be used for monitoring apoptosis and for screening apoptosis inhibitors and activators.Параметрыusage sufficient for 100 fluorometric tests (flow cytometry, orange fluorescence) detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Mitochondrion Membrane Potential Kit MAK147-1KT
NACRES: NA.25 General descriptionMitochondria generate a potential across their membranes due to the activities of enzymes of the electron transport chain. During apoptosis, collapse of the mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.ApplicationMitochondrion Membrane Potential Kit has been used to estimate the mitochondrion membrane potential in chinese hamster ovary (CHO) cells. It has been used in the permeabilization of the mitochondrial membrane test (MMP assay) and also used to detect the loss of mitochondrial membrane permeabilization.SuitabilityThis kit is suitable for the detection of mitochondrial membrane potential (MMP) in mammalian cells and for screening apoptosis inhibitors and activators using microplate readers.PrincipleMitochondrion Membrane Potential Kit is optimized for the detection of the loss of the MMP in cells. The cationic hydrophobic mitochondrial potential dye accumulates in normal mitochondria, most likely due to the mitochondrial potential, resulting in an increase in fluorescence (ex = 540/em = 590 nm). In apoptotic cells, MMP collapse results in decreased fluorescence. This kit can be used for monitoring apoptosis and for screening apoptosis inhibitors and activators.Параметрыusage sufficient for 500 fluorometric tests (orangefluorescence, microplate readers) detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
MMP-1 Inhibitor Screening Kit MAK212-1KT
EC Number: 200-664-3 General descriptionMatrix Metalloproteinase-1 (MMP-1) is a secreted enzyme capable of breaking down interstitial collagen types I, II, and III in the extracellular matrix. Matrix metalloproteinases have important functions in morphogenesis, angiogenesis, apoptosis, tissue remodeling and repair, and tumor metastasis.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the screening of inhibitors of MMP-1.PrincipleThe MMP-1 Inhibitor Screening Kit is a rapid, sensitive and high throughput assay to screen and characterize potential inhibitors of MMP-1. MMP-1 activity is measured by hydrolyzing a FRET-tagged substrate to yield a fluorescent product (ex = 490/em = 520 nm) proportional to the enzymatic activity present.Параметрыdetection method fluorometric storage temp. −20°C Gene Information human ... MMP1(4312)
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
MMP-3 Activity Fluorometric Assay Kit MAK291-1KT
NACRES: NA.84 General descriptionThe matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for measuring MMP-3 activity in various biological samples.PrincipleMatrix metalloproteinase-3 (MMP-3) assay kit, MMP-3 hydrolyzes a specific FRET (fluorescence resonance energy transfer) substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically (lex = 325 nm/lem = 393 nm). The kit provides a rapid, simple, sensitive and reliable test, which can also be used as a high throughput assay of MMP-3 activity. The assay sensitivity is <50 mU.Параметрыdetection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
MMP-3 Inhibitor Screening Kit (Fluorometric) MAK294-1KT
NACRES: NA.25 General descriptionThe matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Similar to several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including Ecadherin, a known contributor to cancer development.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable to screen inhibitors of MMP-3.PrincipleIn the MMP-3 Inhibitor Screening Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically ( λex = 325nm/λem = 393nm). The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput screening assay of MMP-3 inhibition.Параметрыdetection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoamine Oxidase Activity Assay Kit MAK136-1KT
NACRES: NA.84 General descriptionMonoamine oxidases (MAO, EC 1.4.3.4) are a family of mitochondrial enzymes that catalyze the oxidative deamination of monoamines. In mammals, MAO consists of two isoenzymes, MAO-A and MAO-B. MAO dysfunction is thought to be responsible for a number of neurological disorders. Unusually high or low levels of MAO in the body have been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. MAO inhibitors are one of the major classes of drug prescribed for the treatment of depression.ApplicationMonoamine Oxidase Activity Assay Kit has been used to estimate MAO-A (monoamine oxidase A), MAO-B (monoamine oxidase B) and ALDH (aldehyde dehydrogenase) activities.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the determination of MAO activity in biological samples and for the screening of MAO inhibitors. Can determine either MAO-A, MAO-B or both MAO-A and MAO-B activity.PrincipleThe Monoamine Oxidase Assay Kit provides a convenient fluorimetric means to measure MAO enzyme activity in biological samples. In the assay, MAO reacts with p-tyramine, a substrate for both MAO-A and MAO-B, resulting in the formation of H2O2, which is determined by a fluorimetric method (ex = 530/em = 585 nm). The assay is simple, sensitive, stable, and high-throughput adaptable. Unit definition: one unit of MAO catalyzes the formation of 1 µmole of H2O2 per minute under the assay conditions.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. 20°C Gene Information human ... MAOA(4128), MAOB(4129)
mouse ... MAOA(17161), MAOB(109731)
rat ... MAOA(29253), MAOB(25750)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Узнать цену -
Monoamine Oxidase-A Inhibitor Screening Kit (Fluorometric) MAK295-1KT
NACRES: NA.25 General descriptionMonoamine oxidases (MAO, EC 1.4.3.4) are a family of enzymes that can oxidize a wide variety of endogenous primary amines. Two isoforms, MAO-A and MAO-B, have been identified based on their substrate, inhibitor specificity and tissue localization. MAO-B can oxidize primary amines, but its list of specific substrates (i.e. benzylamine, phenylethylamine) is more limited compared to MAO-A.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for screening/studying/characterizing MAO-A inhibitors.PrincipleThis MAO-A Inhibitor Screening Kit offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-A inhibitors. The assay is based on the fluorometric detection of hydrogen peroxide (H2O2), one of the by-products generated during the oxidative deamination of the MAO substrate (tyramine).Параметрыdetection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS06,GHS08 signalword Danger hcodes H301 - H317 - H334 pcodes P261 - P264 - P270 - P280 - P301 + P310 - P302 + P352 RIDADR NONH for all modes of transport Узнать цену -
Monoamine Oxidase-B Inhibitor Screening Kit (Fluorometric) MAK296-1KT
NACRES: NA.25 General descriptionMonoamine oxidases (MAO, EC 1.4.3.4) are a family of enzymes that can oxidize a wide variety of endogenous primary amines. Two isoforms, MAO-A and MAO-B, have been identified based on their substrate, inhibitor specificity and tissue localization. MAO-B can oxidize primary amines, but its list of specific substrates (i.e. benzylamine, phenylethylamine) is more limited compared to MAO-A.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for screening/studying/characterizing MAO-B inhibitors.PrincipleThis MAO-B Inhibitor Screening Kit offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-B inhibitors. The assay is based on the fluorometric detection of H2O2, one of the byproducts generated during the oxidative deamination of the MAO substrate (tyramine).Параметрыdetection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS07,GHS08 signalword Danger hcodes H302 - H317 - H334 - H336 pcodes P261 - P264 - P270 - P280 - P301 + P312 - P302 + P352 Target Organs Central nervous system RIDADR NONH for all modes of transport WGK Germany WGK 3 Узнать цену -
MPO Colorimetric Activity Assay Kit MAK068-1KT
NACRES: NA.84 General descriptionMyeloperoxidase (MPO) is a heme-containing enzyme that catalyzes the hydrogen peroxidase-mediated oxidation of halide ions to hypochlorous acid. MPO is a lysosomal protein, highly expressed in neutrophils, that plays a role in the antimicrobial actions that occur as a result of neutrophil stimulation. The actions of MPO may also contribute to the initiation and pathogenesis of cardiovascular disease.ApplicationMPO Colorimetric Activity Assay Kit has been used to determine the activity of myeloperoxidase in samples.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the measurement of myeloperoxidase (MPO) in biological samples such as tissue, cells, serum and white blood cellsPrincipleIn this assay, MPO catalyzes the formation of hypochlorous acid, which reacts with taurine to form taurine chloroamine. Taurine chloroamine reacts with the chromophore TNB, resulting in the formation of the colorless product DTNB. One unit of MPO activity is defined as the amount of enzyme that hydrolyzes the substrate and generates taurine chloramine to consume 1.0 μmole of TNB per minute at 25 °C.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. 20°C Gene Information human ... MPO(4353)
mouse ... MPO(17523)
rat ... MPO(303413)
Safety Informationpictograms GHS08 signalword Danger hcodes H315 - H319 - H334 pcodes P261 - P264 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
MPO Fluorometric Activity Assay Kit MAK069-1KT
General descriptionMyeloperoxidase (MPO) is a heme-containing enzyme that catalyzes the hydrogen peroxidase-mediated oxidation of halide ions to hypohalous acid. MPO is a lysosomal protein, highly expressed in neutrophils, that plays a role in the antimicrobial actions that occur as a result of neutrophil stimulation. The actions of MPO may also contribute to the initiation and pathogenesis of cardiovascular disease.ApplicationMPO Fluorometric Activity Assay Kit has been used to determine the activity of myeloperoxidase.SuitabilitySuitable for the measurement of myeloperoxidase (MPO) in biological samples such as tissue, cells, serum and white blood cellsPrincipleThe MPO Activity Flurometric Assay kit provides a simple and direct procedure for measuring MPO activity in a variety of samples. In this assay, MPO catalyzes the formation of hypochlorous acid with reacts with the substrate, aminophenyl fluorescein, to generate fluorescein) (ex = 485/em = 525 nm). One unit of MPO is the amount of enzyme that will oxidize the MPO substrate to yield 1.0 µmole of fluorescein per minute at room temperature.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. 20°C Gene Information human ... MPO(4353)
mouse ... MPO(17523)
rat ... MPO(303413)Узнать цену -
MTP Activity Assay Kit MAK110-1KT
NACRES: NA.84 General descriptionMicrosomal triglyceride transfer protein (MTP), a membrane-bound protein present in the liver, plays an important role in the assembly and secretion of very low density lipoprotein (VLDL). MTP is also necessary for assembly and secretion of chylomicrons by the intestine. This protein facilitates the transfer of triglyceride (TG), cholesteryl ester (CE) and phosphatidylcholine (PC) between phospholipid surfaces. Mutation in the MTP gene leads to abetalipoproteinemia.ApplicationMTP Activity Assay Kit has been used to measure the activity of microsomal triglyceride transfer protein (MTP).Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for measuring microsomal [triglyceride] transfer protein (MTP) activity in cell lysates or tissue homogenates, or for screening MTP activity inhibitors.PrincipleThe MTP Activity Assay Kit is a sensitive, homogeneous fluorometric assay useful for continuous measurement of MTP activity in cell lysate or tissue homogenate. This kit includes proprietary substrates that enable the detection of MTP-mediated transfer of neutral lipid. The transfer activity results in an increase in fluorescence intensity (λex=465nm/λem=535nm).Analysis NoteLearn more about Roar Assay Kits for Studies in Lipoprotein Metabolism.,Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. 2-8°C Gene Information human ... MTTP(4547)
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Multidrug Resistance Assay Kit
NACRES: NA.84
Кат. номер MAK161-1EA MAK161-10EA General descriptionAcquired resistance to chemotherapy drugs, multidrug resistance or MDR, is a major contributor to treatment failure for many types of cancers. MDR is typically associated with the increased expression of two ATP-dependent drug efflux pumps, P-Glycoprotein (P-gp or MDR1) and the Multidrug Resistance-Associated Protein (MRP1). These pumps actively expel chemotherapeutic agents, typically hydrophobic amphipathic natural products, from the cytoplasm to exterior of the cell.
This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (ex = 490/em = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.SuitabilityThis kit is suitable for the screening of MDR pump inhibitors or for identifying cell lines with high MDR activity.PrincipleThis kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (ex = 490/em = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.Параметрыusage (Fluorometric MDR Assay) detection method fluorometric storage temp. 20°C Gene Information human ... ABCB1(5243), ABCC2(1244)
mouse ... ABCC2(12780)
rat ... abcc2(25303)
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Myeloperoxidase (MPO) Inhibitor Screening Kit (Fluorometric) MAK283-1KT
General descriptionMyeloperoxidase (MPO: EC 1.11.2.2) is a peroxidase abundantly expressed in neutrophil granulocytes. It catalyzes the hydrogen peroxide dependent oxidation of halide ions to generate hypochlorite (HClO), the reaction by which MPO exhibits cytotoxic activity against tumor cells and microorganisms. MPO also oxidizes various substances such as phenol and anilines. MPO undergoes chlorination or peroxidation reaction depending upon the relative concentrations of chloride and the reducing substrates. Recent studies suggest that increased levels of MPO are associated with an increased risk for cardiovascular disease and myocardial infarction, thus development of novel and specific inhibitors of MPO is critical for therapeutic purposes.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for screening/characterizing/studying potential inhibitors of MyeloperoxidasePrincipleThis MPO Inhibitor Screening Kit provides screening assays for both MPO chlorination and peroxidation activities. In the chlorination assay (specific for MPO), MPO converts hydrogen peroxide and sodium chloride to sodium hypochlorite, which reacts with the chlorination substrate to give an intensely fluorescent product, ex = 480 nm/em = 520. In the peroxidation assay, MPO oxidizes peroxidation substrate to generate fluorescence ex = 535 nm/em = 587. The fluorescence generated is directly proportional to any peroxidase activity present. In the presence of MPO inhibitor, reactions are impeded, thus decreasing the rate and/or extent of generation of MPO-dependent fluorescence.Параметрыdetection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Neprilysin Activity Assay Kit (Fluorometric) MAK350-1KT
General descriptionNeprilysin, also known as neutral endopeptidase, enkephalinase, CD10, and common acute lymphoblastic leukemia antigen, is a zinc-containing transmembrane metalloproteinase. It is able to hydrolyze very important endogenous peptides, such as natriuretic atrial factor, enkephalins, substance P, bradykinin and amyloid β (A β) peptide. Thus, NEP is a potentially therapeutic target in important pathological conditions such as cardiovascular disease, prostate cancer, and Alzheimers disease. NEP has also been used as a biological marker in a type of childhood leukemia. The detection of NEP in endometrial stromal cells has been proposed as a helpful tool in the diagnosis of endometriosis. NEP is currently a focus of major interest in cardiovascular and neurological research.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the measurement of Neprilysin activity in tissue homogenates (lung, kidney, etc.), cell culture (adherent or suspension cells) and purified enzyme.PrincipleThe Neprilysin Activity Assay Kit utilizes the ability of an active NEP to cleave a synthetic substrate (Abz-based peptide) to release a free fluorophore. The released Abz can be easily quantified using a fluorescence microplate reader. The substrate is specific to NEP and can differentiate the NEP activity from Trypsin and other structurally similar zinc metalloproteinase in biological samples such as Angiotensin-Converting Enzymes (ACE1, ACE2), Endothelin Converting Enzymes (ECE1, ECE2). The assay kit is simple, specific and can detect as low as 20 μU/mg of NEP activity.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS09 hcodes H411 pcodes P273 Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
Neuraminidase Activity Assay Kit MAK121-1KT
NACRES: NA.84 General descriptionNeuraminidase, also known as Sialidase, is an enzyme that hydrolyzes terminal sialic acid residues on poly-saccharide chains. It is predominantly expressed in microorganisms such as bacteria and viruses. Cleavage of sialic acid residues by neuraminidase is believed to play several roles in infection by influenza viruses. It is thought to assist in the penetration of mucosal linings, the invasion of target cells, the elution of progeny viruses from infected cells, and the prevention of self-aggregation. Thus, neuraminidase is an important target for influenza drug development and simple, direct, and automation-ready procedures for measuring neuraminidase activity find wide applications in research and drug discovery.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the detection of neuraminidase activity in biological samples and for the screening of neuraminidase inhibitors.PrincipleIn this assay, neuraminidase activity is determined by an enzymatic assay in which sialic acid released by neuraminidase results in a colorimetric (570 nm)/fluorometric (λex = 530/λem = 585 nm) product directly proportional to neuraminidase activity in the sample. The linear detection range at 37 °C is 0.1-10 units/L for colorimetric assays and 0.01-2 units/L for fluorometric assays.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric shipped in dry ice storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H334 pcodes P261 - P284 - P304 + P340 + P312 - P501 RIDADR NONH for all modes of transport Узнать цену -
Neutrophil Elastase Activity Assay Kit (Fluorometric) MAK246-1KT
NACRES: NA.25 General descriptionNeutrophil Elastase (NE, EC 3.4.21.37, leukocyte elastase, ELANE, ELA2, elastase 2, neutrophil elaszym, or serine elastase) is a cytotoxic serine protease. It is stored in the azurophil granules of neutrophil granulocyte and is released following cell stimulation, e.g. by pathogens, immune complexes or chemotactic agents (PMA). Mutation in the gene ELA2 (neutrophil elastase) results in neutropenia, a hereditary disorder characterized by reduced neutrophil count in an individual.SuitabilitySuitable to determine the activity of purified Neutrophil Elastase, detect the activity of Neutrophil Elastase in plasma and/or blood and screen/study/characterize chemotactic agents causing Neutrophil stimulation and release of Elastase.PrincipleThe Neutrophil Elastase Activity Assay Kit utilizes the ability of NE to proteolytically cleave a synthetic substrate and release a fluorophore, AFC, which can be easily quantified by fluorescence. This assay kit is simple, rapid, and can detect as low as 1 ng of neutrophil elastase in a variety of samples.Параметрыdetection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS07,GHS08 signalword Danger hcodes H315 - H319 - H334 - H335 pcodes P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338 Target Organs Respiratory system RIDADR NONH for all modes of transport Узнать цену -
Neutrophil Elastase Inhibitor Screening Kit MAK213-1KT
EC Number: 232-670-7 NACRES: NA.25 General descriptionNeutrophil elastase (NE) is a serine protease that hydrolyzes proteins within the azurophilic granules of the neutrophils. Once secreted, it digests collagen-VI and elastin of the extracellular matrix. Neutrophil elastase is implicated in cystic fibrosis, bronchiectasis, chronic obstructive pulmonary disease, congenital neutropenia, and lung cancer.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the screening of inhibitors of Neutrophil Elastase.PrincipleThe Neutrophil Elastase Inhibitor Screening Kit is a rapid, sensitive and high throughput assay to screen and characterize potential inhibitors of NE. NE activity is measured by hydrolyzing the substrate to yield a fluorescent product (ex = 400/em = 505 nm) proportional to the enzymatic activity present.Параметрыdetection method fluorometric storage temp. 20°C Gene Information human ... ELANE(1991)
mouse ... ELANE(50701)
rat ... ELANE(299606)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Узнать цену -
Nickel Assay Kit MAK027-1KT
NACRES: NA.84 General descriptionNickel acts as a cofactor for several enzymes, including some ureases, carbon monoxide dehydrogenases (methane forming enzymes which reduce CO2 to CH4), and some hydrogenases. Nickel forms complexes with sulfhydryl compounds with significant absorbance in the UV/visible region in the presence of other ions.
Nickel acts as a cofactor for several enzymes, including some ureases, carbon monoxide dehydrogenases (methane forming enzymes which reduce CO2 to CH4), and some hydrogenases. Nickel forms complexes with sulfhydryl compounds with significant absorbance in the UV/visible region in the presence of other ions.
In the Nickel Assay kit, Ni2+ reacts with 2-mercaptoethanol in borate buffer to form a complex with strong absorbance bands from 300 to 600 nm. Fe2+ and Co2+ interfere with the assay; therefore, extra steps must be taken to subtract the interference in order to determine the correct nickel concentration in mixed samples. Other ions tested (Mn2+, Cu2+, and Zn2+) do not interfere with the assay and presumably no other ionic species will be present in high enough concentration to interfere with the reaction. The assay is a simple method of quantifying Ni2+ in a variety of samples, which gives a linear range of 2–50 nmole nickel in samples containing <25 nmole of Co2+ ions.SuitabilitySuitable for quantifying nickel concentrations in a variety of samples.PrincipleIn the Nickel Assay kit, Ni2+ reacts with 2-mercaptoethanol in borate buffer to form a complex with strong absorbance bands from 300 to 600 nm. Fe2+ and Co2+ interfere with the assay; therefore, extra steps must be taken to subtract the interference in order to determine the correct nickel concentration in mixed samples. Other ions tested (Mn2+, Cu2+, and Zn2+) do not interfere with the assay and presumably no other ionic species will be present in high enough concentration to interfere with the reaction. The assay is a simple method of quantifying Ni2+ in a variety of samples, which gives a linear range of 2–50 nmole nickel in samples containing <25 nmole of Co2+ ions.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. 2-8°C
Safety Informationpictograms GHS07,GHS08 signalword Danger hcodes H317 - H350i - H373 - H412 pcodes P201 - P273 - P280 - P308 + P313 RIDADR NONH for all modes of transport Узнать цену -
NIR Mitochondrion Membrane Potential Kit MAK149-1KT
NACRES: NA.25 General descriptionMitochondria generate a potential across their membranes due to the activities of enzymes of the electron transport chain. During apoptosis, collapse of the mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.SuitabilityThis kit is suitable for the detection of Mitochondrial Membrane Potential in mammalian cells and for screening apoptosis inhibitors and activators using microplate readers.PrincipleNIR Mitochondrion Membrane Potential Kit is optimized for the detection of the loss of the MMP in cells. The cationic hydrophobic mitochondrial potential dye accumulates in normal mitochondria, most likely due to the mitochondrial potential, resulting in an increase in fluorescence (λex = 640/λem = 680 nm). In apoptotic cells, MMP collapse results in decreased fluorescence. This kit can be used for monitoring apoptosis and for screening apoptosis inhibitors and activators.Параметрыusage sufficient for 500 fluorometric tests (near infrared fluorescence, microplate readers) detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Nitric Oxide Synthase Inhibitor Screening Kit MAK323-1KT
General descriptionNitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO is the oxidation product of arginine by nitric oxide synthase (NOS) and is involved in host defense development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Inhibition of NOS has the potential to produce diverse biological effects, particularly in the cardiovascular system. Simple, direct and non-radioactive procedures for measuring NOS are becoming popular in research and drug discovery.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for inhibitor screening and evaluation of nitric oxide synthase inhibitors.PrincipleThe Nitric Oxide Synthase Inhibitor Screening Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following reduction of nitrate to nitrite using an improved Griess method.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric
Safety Informationpictograms GHS05,GHS07 signalword Warning hcodes H290 - H302 - H315 - H319 pcodes P234 - P264 - P270 - P301 + P312 - P302 + P352 - P305 + P351 + P338 Узнать цену -
Nitrite Assay Kit (Griess Reagent) MAK367-1KT
General descriptionNitrogen-based ions, nitrite and nitrate, are found in almost every living organism. Furthermore, endogenous Nitrite levels are found in mammals and can also be obtained from dietary sources. In humans, nitrite is further metabolized to Nitric Oxide and other reactive nitrogen species (nitrogen oxides). The Nitrate-Nitrite-NO biochemical pathway is well known for its participation in cell signaling, hypoxiadependent response and regulation of blood flow. Recent studies suggest the reduction of Nitrite to Nitrogen Oxygen in the mitochondria. Specifically, myoglobin and xanthine oxidoreductase could generate NO under hypoxic conditions leading to mitochondrial respiration.SuitabilityThe kit is suitable for the detection of Nitric Oxide in animal tissues (liver, kidney, etc.), cell culture (adherent or suspension cells), serum, plasma and urine.PrincipleThe Nitrite Assay Kit utilizes the Griess Reagent, a classic protocol for the estimation of nitrite. In the assay, nitrite is reduced to Nitrogen Oxide using Griess Reagent I. Then, Nitrogen Oxide reacts with Griess Reagent II forming a stable product that can be detected by its absorbance at A540 nm. The one-step assay is simple, fast and can detect nitrite levels as low as 1 nmol/well.Параметрыusage sufficient for 200 colorimetric tests detection method colorimetric storage temp. 2-8°C
Safety Informationpictograms GHS05 signalword Danger hcodes H290 - H314 pcodes P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
NNMT Inhibitor Screening Assay Kit MAK299-1KT
NACRES: NA.84 General descriptionN′-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and diabetes, indicating it is a potential molecular target for therapy.SuitabilitySuitable for screening/studying/characterizing potential inhibitors of N′-Nicotinamide Methyltransferase (NNMT).PrincipleThis NNMT inhibitor screening kit utilizes SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH hydrolase to form homocysteine, the free thiol group of which is detected using the Thiol Detecting Probe, generating an enhanced fluorescence signal (λex = 392 nm/ λem = 482 nm). In the presence of an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of NNMT.Параметрыdetection method fluorometric storage temp. −70°C
Safety Informationpictograms GHS02,GHS05,GHS07,GHS08 signalword Danger hcodes H226 - H290 - H312 - H314 - H317 - H361fd - H412 pcodes P201 - P210 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338 + P310 RIDADR UN 3316 9 Flash Point F 84.2 °F Flash Point C 29 °C Узнать цену -
Oxalate Assay Kit MAK315-1KT
NACRES: NA.84 General descriptionOxalate or Oxalic Acid is a metabolic breakdown product of the Krebs Cycle in eukaryotes, and the glyoxylate cycle in other microorganisms. It can be found in the urine of humans and other mammals. Oxalate concentration can be used as a measure of kidney function where a high level of oxalate is an indicator for kidney stones which are primarily made of the insoluble salt calcium oxalate. Measuring oxalate is more accurate than measuring calcium as a marker for kidney stones because calcium is excreted at high concentrations even in normal urine.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the quantitative determination of oxalate (oxalic acid) in urine, animal and plant tissue samples.PrincipleSimple, direct and high-throughput assays for measuring oxalate concentration find wide applications. This oxalate assay kit uses a single Working Reagent that combines the oxalate oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 595 nm is directly proportional to oxalate in the sample.Параметрыdetection method colorimetric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Oxalate Decarboxylase Activity Assay Kit MAK214-1KT
EC Number: 200-550-3 NACRES: NA.84 General descriptionOxalate Decarboxylase (OXDC, EC 4.1.1.2) catalyzes the conversion of oxalate to formate and carbon dioxide in the presence of molecular oxygen. OXDC-responsive proteins are involved in the regulation of metabolism and stress responses in plants.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the detection of Oxalate Decarboxylase activity in samples such as plants and fungi.PrincipleThe Oxalate Decarboxylase Activity Assay Kit provides a simple and high throughput adaptable procedure for measuring OXDC activity in a variety of plant and fungal tissues. OXDC activity is determined by generating a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of oxalate decarboxylase is the amount of enzyme required to generate 1.0 μmole of formate per minute at pH 5 at 37 °C.Параметрыdetection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H302 + H312 pcodes P264 - P270 - P280 - P301 + P312 - P302 + P352 + P312 - P362 + P364 RIDADR NONH for all modes of transport Узнать цену -
Oxalic Acid Colorimetric Assay Kit MAK179-1KT
EC Number: 200-550-3 NACRES: NA.84 General descriptionOxalic acid (Oxalate) is a strong dicarboxylic acid that occurs in several plants and vegetables. In animals, it is either incorporated from the diet or produced during ascorbic acid and glyoxylic acid metabolism. Oxalic acid is not metabolized and is excreted in the urine. Hence, it can act as a marker for glyoxylic and ascorbic acid metabolism. Furthermore, oxalic acid has been studied as a pathogenic factor for Sclerotinia sclerotiorum that inhibits the oxidative burst response in host plants. It is also known to alleviate chilling injury in peach fruit. Elevated levels of oxalic acid (oxalate) have been associated with the formation of kidney stones. Hence, oxalate measurements can be used for the diagnosis and monitoring of renal stones.ApplicationOxalic acid colorimetric assay kit has been used in 24-hour urinary oxalate level (24HUOL) evaluation in an animal model.SuitabilitySuitable for the detection of oxalate (oxalic acid) in biological samples including animal and plant tissue.PrincipleThe Oxalic Acid Assay Kit provides a simple and sensitive procedure for measuring oxalic acid in variety of samples including animal and plant tissue. In this assay, oxalic acid concentration is determined by a coupled enzyme reaction, which results in a colorimetric (450 nm) product, proportional to the oxalate present.Параметрыdetection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H302 + H312 pcodes P264 - P270 - P280 - P301 + P312 - P302 + P352 + P312 - P362 + P364 RIDADR NONH for all modes of transport Узнать цену -
Oxaloacetate Assay Kit MAK070-1KT
NACRES: NA.84 General descriptionOxaloacetate (OAA) is an intermediate of both the TCA cycle and gluconeogenesis. It precedes citrate which is formed by the citrate synthase-mediated condensation of acetyl-CoA and OAA. OAA is also formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl-CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase. The Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples.ApplicationOxaloacetate Assay Kit has been used to determine the concentration of oxaloacetate in samples.SuitabilitySuitable for animal tissues, cell culture (adherent or suspension cells), serum and saliva.PrincipleIn this kit, oxaloacetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the oxaloacetate present. Typical detection ranges for this kit are 2–10 nmole (colorimetric) and 0.2–1 nmole (fluorometric).Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C Узнать цену -
PAH (p-Aminohippuric Acid) Assay Kit MAK101-1KT
NACRES: NA.25 General descriptionp-Aminohippuric acid (PAH), a derivative of hippuric acid, is used in the measurement of renal plasma flow (RPF). Renal plasma flow measures the volume of plasma, which enters the kidney in a given period of time. To determine effective renal plasma flow, PAH is measured in a plasma sample (PPAH) and a urine sample (UPAH). The volume of urine flow (V) is also required.SuitabilitySuitable for measuring PAH (p-Aminohippuric Acid) in plasma and urine samplesPrincipleThe PAH (p-Aminohippuric Acid) Assay kit can be used for measuring PAH in a plasma and urine samples. PAH reacts with 4-(dimethylamino)cinnamaldehyde (DACA) to generate a compound, which can be detected spectrophotometrically at 550 nm.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05,GHS07,GHS09 signalword Danger hcodes H314 - H335 - H410 pcodes P261 - P273 - P280 - P305 + P351 + P338 - P310 - P501 Target Organs Respiratory system RIDADR UN 3316 9 Узнать цену -
PDH Activity Assay Kit MAK183-1KT
NACRES: NA.84 General descriptionPyruvate dehydrogenase (PDH) is a mitochondrial enzyme that catalyzes the conversion of pyruvate to acetyl-CoA and CO2, and also links the tricarboxylic acid (TCA) and glycolysis pathways. The enzyme is inhibited by phosphorylation and activated by dephosphorylation. Mutations in PDH have been linked to pyruvate dehydrogenase deficiency (causing lactic acidosis and neurologic dysfunctions) and Leigh syndrome. PDH has also been implicated in oncogene-induced senescence. PDH measurements can provide insights into metabolic functions and oncogenesis.ApplicationPDH Activity Assay Kit has been used to determine the activity of pyruvate dehydrogenase.SuitabilitySuitable for the detection of PDH activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.PrinciplePyruvate dehydrogenase activity is determined using a coupled enzyme reaction, which results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of pyruvate dehydrogenase is the amount of enzyme that will generate 1.0 mmole of NADH per minute at 37 °C.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. 20°C Gene Information human ... PDHA1(5160), PDHA2(5161), PDHB(5162), PDHX(8050)
mouse ... PDHA1(18597), PDHA2(18598), PDHB(68263), PDHX(27402)
rat ... PDHA1(29554), PDHA2(117098), PDHB(289950), PDHX(311254)
Safety Informationpictograms GHS05,GHS07 signalword Danger hcodes H314 - H317 pcodes P261 - P272 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 RIDADR NONH for all modes of transport Узнать цену -
PEG Virus Precipitation Kit MAK343-1KT
General descriptionVirus is typically produced at a low titer. It often needs to be concentrated for storage and/or for use in further applications.SuitabilitySuitable to concentrate retroviruses, baculoviruses, lentiviruses, and phages etc. in cell culture medium or environmental samples.Параметрыusage sufficient for 200 preparations storage temp. −20°C
Safety InformationFlash Point F Not applicable Flash Point C Not applicable Узнать цену -
PEP Colorimetric/Fluorometric Assay Kit MAK102-1KT
EC Number: 215-185-5 NACRES: NA.84 General descriptionPhospho(enol)pyruvate (PEP) in an intermediate in carbohydrate metabolism involved in glycolysis and gluconeogeneis. In glycolysis, PEP is metabolized by pyruvate kinase to yield pyruvate. In plants, PEP is involved in the formation of aromatic amino acids as well as in the carbon fixation pathway.ApplicationPEP Colorimetric/Fluorometric Assay Kit has been used to measure the concentrations of phosphoenolpyruvate.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the measurement of phosphoenolpyruvate (PEP) in tissue, cells and plant extractsPrinciplePEP concentration is determined by a coupled enzyme assay, in which PEP is converted to ATP and pyruvate, resulting in a colorimetric (570 nm)/fluorometric (ex = 535/em = 587 nm) product, proportional to the PEP present.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 pcodes P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312 RIDADR UN 3316 9 Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Peroxidase Activity Assay Kit MAK092-1KT
NACRES: NA.84 General descriptionPeroxidase (EC 1.11.1.7) is an enzyme found broadly in biological systems that utilizes hydrogen peroxide in the oxidation of various substrates. The Peroxidase Activity Assay Kit provides a simple and direct procedure for measuring peroxidase activity in a variety of biological samples.ApplicationPeroxidase Activity Assay Kit has been used to quantify the activity of peroxidase.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the determination of peroxidase activity in cell culture supernatant, serum, plasma, urine, and other biological fluids.PrinciplePeroxidase catalyzes the reaction between H2O2 and the probe, resulting in a colorimetric (570 nm)/fluorometric (ex = 535/em = 587 nm) product, proportional to the peroxidase activity present. One unit of peroxidase is defined as the amount of enzyme that reduces1.0 µmole of H2O2 per minute at 37°C.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 pcodes P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312 RIDADR NA 1993 / PGIII Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену
Получите коммерческое предложение в течение 1 часа
Менеджер подготовит коммерческое предложение и позвонит, если понадобится уточнить детали вашего заказа
Анна Гловацкая
Менеджер по работе с клиентами
С 2010 года мы поставляем оборудование с заводов Европы. Берем на себя все — от подбора оборудования до внедрения на предприятии
Все сотрудники имеют высшее образование, закончили ведущие химические вузы страны, такие как РХТУ им Менделеева.
У большинства компаний срок ожидания составляет 10-12 недель.
Оборудование хранится на сухом отапливаемом складе, где поддерживается ровная температура.
Работаем с PonyExpress и Деловыми линиями. Вы также можете выбрать свою транспортную компанию или забрать товар со склада в Москве.
В случае любых неполадок за свой счет выполним ремонт в сервисном центре или на заводе-изготовителе. Или бесплатно заменим прибор на новый.
Производим пуско-наладку оборудования, валидацию, обучение сотрудников. Если нужно, привлекаем инженеров с заводов- изготовителей.
