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Fluorometric Sphingomyelinase Assay Kit MAK155-1KT
NACRES: NA.84 General descriptionSphingomyelinase (SMase), or sphingomyelin phosphodiesterase, catalyzes the hydrolytic cleavage of sphingolipid to phosphocholine and ceramide, a second messenger involved in multiple cellular responses including the cellular response to stress. There are at least five types of SMases identified, which are classified according to their cation dependence, cellular location, and pH optima for activity, and include the acidic and neutral sphingomyelinase. Neutral SMases, along with the acidic SMases, are thought to be major contributors to the production of ceramide in response to cellular stress. Mutations in is associated with metabolic disorders like Niemann-Pick disease B.SuitabilityThis kit is suitable for the detection of sphingomyelin in a variety of samples such as blood and cell extracts.PrincipleThe Sphingomyelinase Assay Kit provides a simple and direct procedure for measuring neutral SMase activity in a variety of samples such as blood or cell extracts, or for screening SMase activators and inhibitors. SMase activity is determined by a coupled enzyme assay, which results in a fluorescent product (λex =540/λem = 590 nm) proportional to the SMase activity present.Параметрыusage sufficient for 200 fluorometric tests (red fluorescence) detection method fluorometric storage temp. 20°C Gene Information human ... SMPD2(6610), SMPD3(55512), SMPD4(55627)
mouse ... SMPD2(20598), SMPD3(58994), SMPD4(77626)
rat ... SMPD2(83537), SMPD3(94338), SMPD4(303790)
Safety Informationpictograms GHS05,GHS08,GHS09 signalword Danger hcodes H317 - H318 - H334 - H410 pcodes P261 - P273 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 RIDADR NONH for all modes of transport Узнать цену -
Fluorometric Thiol Assay Kit MAK151-1KT
EC Number: 200-664-3 NACRES: NA.84 General descriptionThiol groups, found as free cysteine, glutathione (GSH), and cysteine residues in proteins, are involved in many biological processes. The disulfide bonds generated when the thiol groups of two cysteine residues are oxidized contribute to the tertiary or quatenary structure of a protein.ApplicationFluorometric Thiol Assay Kit has been used to determine the lipid peroxidation expressed as thiols in samples.SuitabilityThis kit is suitable for the detection of thiols in a variety of different biological samples including plasma, urine, and cell extracts. This kit is not optimized for measuring thiol groups in proteins.PrincipleThe Fluorometric Thiol Quantitation Kit provides an ultrasensitive assay to quantitate the thiol content of small molecules. The proprietary thiol sensor generates a strongly fluorescent adduct (λex = 490/λem = 520 nm) upon reacting with a thiol-containing compound. In addition, both absorption and emission spectra of the thiol adduct are pH-independent, making this assay kit highly robust. The kit can detect as little as 1 picomole of cysteine or GSH in a 100 μL assay volume (10 nM). The assay can be performed conveniently in either a 96 or 384 multiwell plate format.Параметрыusage sufficient for 200 fluorometric tests detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Formaldehyde Assay MAK131-1KT
NACRES: NA.84 General descriptionFormaldehyde is widely employed in industry for a wide range of applications. It acts as a disinfectant and biocide, and is utilized as a tissue fixative and embalming agent. Formaldehyde is a key metabolite produced during normal metabolism and contributes to methylation reactions and protein and nucleic acid synthesis. Increased production of formaldehyde has been observed in many cancer cell lines and increased formaldehyde concentration in urine has been associated with bladder and prostrate cancer.Features and BenefitsCompatible with high-throughput handling systems. Can be adapted for use with cuvettes.SuitabilitySuitable for the detection of formaldehyde in urine and other biological samples.PrincipleThe Formaldehyde Assay kit provides a simple and direct procedure for measuring formaldehyde levels in urine and other biological samples. In this assay, formaldehyde is derivatized with acetoacetanilide in the presence of ammonia resulting in a fluorescent (λex = 370/λem = 470 nm) product, proportional to the formaldehyde present.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. 2-8°C
Safety Informationpictograms GHS05,GHS07,GHS08,GHS09 signalword Danger hcodes H290 - H302 - H314 - H335 - H370 - H410 pcodes P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 Target Organs Respiratory system RIDADR UN 3316 9 WGK Germany WGK 3 Узнать цену -
Formate Assay Kit
NACRES: NA.84
Кат. номер MAK059-1KT MAK059-1KT-CUS General descriptionFormate, the salt form of formic acid, is the simplest carboxylate anion. Formate is produced in the mitochondria as a result of the oxidation of one-carbon donors such as serine. Mitochondrially-derived formate is utilized for cytoplasmic one-carbon metabolism in the de novo synthesis of purines, thymdiylate, and the remethylation of homocysteine to methionine. Under normal physiologic conditions, formate is present at low levels in serum and blood but levels may be increased following acute methanol poisoning or environmental exposure to formaldehyde. Serum and urine formate levels were increased in vitamin B12-deficient rats and may be indicative of hyperhomocysteinemia due to defects in remethylation.ApplicationFormate Assay kit has been used to determine the concentration of formate in samples.SuitabilitySuitable for serum, urine, media, cells, and tissues.PrincipleThe Formate Assay Kit provides a simple, sensitive, and rapid means of quantifying formate in a variety of samples including serum, urine, media, cells, and tissues. Formate concentrations are determined by an enzymatic assay, which results in a colorimetric (450 nm) product, proportional to the formate present.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05 signalword Danger hcodes H314 pcodes P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 - P405 RIDADR NONH for all modes of transport Узнать цену -
Free Fatty Acid Quantitation Kit MAK044-1KT
General descriptionFree fatty acids, also know as nonesterified fatty acids, circulate in the plasma bound to albumin. The majority of free fatty acids are derived from either dietary sources or are mobilized from adipose tissue. Plasma free fatty acids are elevated in many obesity-related disorders and may contribute to insulin resistance in peripheral tissues. Conditions such as sepsis and tumors producing lipoactive homones may also be associated with elevated free fatty acid levels.
Abnormalities in fatty acid metabolism causes fatty liver disease. Accumulation of free fatty acid in myocytes leads to the risk of cardiac dysfunction. Increased fatty acid -oxidation is implicated in cardiac ischemia.ApplicationFree Fatty Acid Quantitation Kit has been used in the following:- to measure lipid hydrolysis, by determining the free fatty acid in digested sample.
- to measure plasma and serum free fatty acids level.
- to quantify free fatty acids level in liver tissue.
SuitabilitySuitable for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc.PrincipleIn this kit, the concentration of fatty acids (C8 and longer) is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (ex = 535/em = 587 nm) product, proportional to the fatty acids present.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety InformationPictograms GHS07,GHS09 Signal Word Warning Hazard Statements H319 - H411 Precautionary Statements P264 - P273 - P280 - P337 + P313 - P391 - P501 RIDADR NA 1993 / PGIII WGK Germany WGK 3 Flash Point(F) 188.6 °F - closed cup Flash Point(C) 87 °C - closed cup Узнать цену -
Free Glycerol Determination Kit FG0100-1KT
NACRES: NA.26 General descriptionGlycerol, also referred to as glycerin or glycerine, is a 3 carbon sugar alcohol that forms the backbone of fatty acids. It is a central component for synthesis of all lipids, and acts as a backbone for triglycerides and phospholipids, which play an important role in cell membrane structure. Due to its low toxicity, glycerol is widely used in pharmaceutical, food and cosmetic formulations and is the main waste by-product of biodiesel production via transesterification. In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed.ApplicationThe triglyceride and free glycerol kits are for the quantitative determination of glycerol, total triglycerides or free triglycerides.
The Free Glycerol Determination Kit has been used to determine the level of glycerol released into the medium from mammalian cells grown in vitro .
The Free Glycerol Determination Kit measures free, endogenous glycerol using coupled enzyme reactions and does not include initial lipase hydrolysis. Glycerol is phosphorylated by adenosine-5-triphosphate (ATP) forming glycerol-1-phosphate (G-1-P) and adenosine-5-diphosphate (ADP) in the reaction catalyzed by glycerol kinase (GK). G-1-P is then oxidized by glycerol phosphate oxidase (GPO) to dihydroxyacetone phosphate (DAP) and hydrogen peroxide (H2O2). Peroxidase catalyzes the coupling of H2O2 with 4-aminoantipyrine (4-AAP) and sodium N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to the free glycerol concentration of the sample.SuitabilitySuitable for the quantitative enzymatic determination of glycerol in serum or plasma.LinkageIn addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed.ПараметрыQuality Level 200, usage 1 kit sufficient for 1000 reactions detection method colorimetric storage temp. 2-8°C
Safety Informationpictograms GHS05,GHS07,GHS09 signalword Danger hcodes H302 - H315 - H318 - H410 pcodes P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 RIDADR UN 3077 9 / PGIII Узнать цену -
Fructose Colorimetric/Fluorometric Assay Kit MAK265-1KT
NACRES: NA.84 General descriptionFructose is a monosaccharide naturally found in honey and fruits. Several studies have linked fructose to the development of the metabolic syndrome particularly through promoting insulin resistance in the liver. It has also been implicated in the obesity epidemic. Studies in animals have revealed high fructose intake during pregnancy and lactation may lead to metabolic dysfunctions in the mother and the newborn. Fructose measurements can provide useful insights into metabolic and biochemical functions. Excessive uptake of fructose is implicated in obesity in children. Fructose induced hyperuricemia, reduces nitric oxide bioavailability for insulin and thus it is implicated in diabetes. Fructose might be implicated in cardiovascular disease, as it is the only sugar that raises uric acid production in the body. High fructose diet is known to induce hypertension.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for detecting fructose in cell and tissue culture supernatants. Note: Not suitable for urine, plasma, or serum.PrincipleIn this assay kit, fructose is converted to -glucose resulting in a colorimetric (570 nm)/fluorometric (ex = 535 nm/em = 587 nm) product proportional to fructose present. This kit has a linear range of detection between 0.2–1.0 nmole fructose for the fluorometric assay and 2–10 nmole fructose for the colorimetric assay.Параметрыdetection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 - H412 pcodes P261 - P272 - P273 - P280 - P284 - P302 + P352 RIDADR UN 3316 9 WGK Germany WGK 3 Узнать цену -
Fructose-6-Phosphate Assay Kit MAK020-1KT
NACRES: NA.84 General descriptionFructose-6-phosphate (F6P) is a glycolytic pathway intermediate produced by the isomerization of glucose-6-phosphate by phosphoglucose isomerase. F6P is further phosphorylated to fructose 1,6-biphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. F6P can also be shunted from glycolysis to the non-oxidative branch of the pentose phosphate pathway as a means of generative pentose phosphates for nucleotide synthesis. F6P levels are elevated in rapidly proliferating cells such as cancer cells. The Fructose-6-phosphate Assay Kit is a highly sensitive and simple fluorescence-based method of quantifying F6P in a variety of samples.ApplicationFructose-6-Phosphate Assay Kit has been used to measure fructose-6-phosphate (F-6-P) and glucose-6-phosphate (G6P).SuitabilitySuitable for quantifying Fructose-6-Phosphate in a variety of samplesPrincipleFructose-6-phosphate concentration is determined by a coupled enzyme reaction, which results in a fluorometric (ex = 535 nm/em = 587 nm) product, proportional to the fructose-6-phosphate present. Typical sensitivities for this kit are 0.01 to 0.5 nmoles of F6P.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NA 1993 / PGIII Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Fumarase Activity Colorimetric Assay Kit MAK206-1KT
NACRES: NA.84 General descriptionFumarase (Fumarate Hydratase; EC 4.2.1.2) catalyzes the reversible hydration of fumarate to malate in the tricarboxylic acid (Krebs) cycle. The activity of fumarase is important as it produces reducing equivalents that drive the synthesis of ATP and maintains energy balance in aerobic metabolism. A deficiency of fumarase activity results in progressive encephalopathy.ApplicationFumarase Activity Colorimetric Assay Kit has been used to determine fumarase (FUM) activity.SuitabilitySuitable for the detection of Fumarase in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.PrincipleThe Fumarase Activity Colorimetric Assay Kit provides a simple and high-throughput ready procedure for measuring fumarase activity in a variety of tissues, cells, and isolated mitochondria. Fumarase activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of fumarase is the amount of enzyme that generates 1.0 mmole of NADH per minute at pH 9.5 at 37 °C.Параметрыdetection method colorimetric storage temp. 20°C Gene Information human ... FH(2271)
mouse ... FH(14193)
rat ... FH(24368)
Safety Informationpictograms GHS05 signalword Danger hcodes H314 pcodes P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 - P405 RIDADR NONH for all modes of transport Узнать цену -
Fumarate Assay Kit MAK060-1KT
General descriptionFumarate is a tricarboxylic acid (TCA) cycle intermediate formed by the oxidation of succinate by succinate dehydrogenase. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. Deficiencies in fumarate hydratase are associated with increased cellular concentrations of fumarate and increased incidences of renal cell cancer. This is most likely due to fumarate-mediated upregulation of HIF1. The Fumarate Assay Kit provides a convenient tool for sensitive detection of the fumarate in a variety of samples.ApplicationFumarate Assay Kit has been used to determine the concentration of fumarate in samples.SuitabilitySuitable for the detection of fumarate in a variety of samples including tissue, cells and serumPrincipleFumarate is determined by an enzyme assay, which results in a colorimetric (450 nm) product, proportional to the fumarate present.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationhcodes H412 pcodes P273 - P501 RIDADR NONH for all modes of transport Узнать цену -
Galactose and Lactose Assay Kit MAK011-1KT
NACRES: NA.84 General descriptionLactose is a disaccharide containing one galactose and one glucose molecule. Lactose is the major sugar in the milk of most species, typically present between 2-8%. The enzyme lactase hydrolyzes lactose to its constituent monosaccharides. Galactose is a simple monosaccharide that serves as an energy source and as an essential component of glycolipids and glycoproteins. Galactose contributes to energy metabolism via its conversion to glucose by the enzymes that constitute the Leloir pathway. Defects in the genes encoding these proteins leads to the metabolic disorder galactosemia.ApplicationGalactose and lactose assay kit has been used in the measurement of lactose/galactose concentration in milk/lactose-free milk and to estimate the saccharides concentrations measured with the biosensor.SuitabilitySuitable for use with various biological samples including serum, plasma, other body fluids, food, and growth media.PrincipleIn this assay kit, galactose is oxidized by galactose oxidase resulting in a colorimetric (570 nm)/ fluorometric (ex = 535 nm/em = 587 nm) product, proportional to the galactose present. To detect lactose, lactose is hydrolyzed by lactase to generate free galactose, which is then measured (total galactose). The lactose level is equal to total galactose minus free galactose.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 - H412 pcodes P261 - P272 - P273 - P280 - P284 - P302 + P352 RIDADR NA 1993 / PGIII Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Galactose Assay Kit MAK012-1KT
NACRES: NA.84 General descriptionGalactose is a simple monosaccharide that serves as an energy source and as an essential component of glycolipids and glycoproteins. Galactose contributes to energy metabolism via its conversion to glucose by the enzymes that constitute the Leloir pathway. Defects in the genes encoding these proteins lead to the metabolic disorder galactosemia.Application- Galactose assay kit has been used in the determination of reactor galactose concentration.
- It has been used in D-galactose assay using the galactose oxidase enzymatic microreactor.
- It has also been used in the general synthesis and characterization of O-(2-aminoethyl)-O-galactosyl polyethylene glycol (H2N-PEG-GAL) derivative.
SuitabilityThis kit is suitable for use with various biological samples including serum, plasma, other body fluids, food, and growth media.PrincipleIn this assay kit, Galactose is oxidized resulting in a colorimetric (570 nm)/fluorometric (ex = 535 nm/em = 587 nm) product, proportional to the galactose present. This kit has a linear range of detection between 0.2-1.0 nmole galactose for the fluorometric assay and 2-10 nmole galactose for the colorimetric assay.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 - H412 pcodes P261 - P272 - P273 - P280 - P284 - P302 + P352 Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
GAPDH Activity Assay Kit MAK277-1KT
NACRES: NA.84 General descriptionGAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase; EC 1.2.1.12) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH (housekeeping gene) expression is stable and constitutive. Deregulation of GAPDH activity is associated with abnormal cell proliferation and carcinogenesis. Accurate quantitation of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology.ApplicationGAPDH Activity Assay Kit has been used to measure the enzyme activity of glyceraldehyde-3-phosphate dehydrogenase in testis.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for use with various tissue and cell culture samples.PrincipleThe GAPDH Activity Assay Kit provides a simple and sensitive method for monitoring GAPDH activity in various samples. GAPDH activity is determined in a coupled enzyme reaction in which GAP is converted to BPG by GAPDH. This results in a colorimetric (450 nm) product proportional to the enzymatic activity present. The assay is sensitive to 100 mUnits/mL. One unit of GAPDH activity is the amount of enzyme that will generate 1.0 mmole of NADH per minute at pH 7.2 at 37 °C.Параметрыdetection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05 signalword Danger hcodes H314 - H412 pcodes P273 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 RIDADR NONH for all modes of transport Узнать цену -
Gelatinase (Gelatin Degradation/Zymography) Assay Kit (Fluorometric) MAK348-1KT
General descriptionGelatinases are a type of matrix zinc-dependent metalloproteases (MMPs) that degrade gelatins and a variety of other extracellular matrix proteins. These enzymes are synthesized as latent zymogens that are activated by proteolysis and inhibited by tissue inhibitors of metalloproteases (TIMPs). Two mammalian gelatinases, Gelatinase A (MMP-2) and Gelatinase B (MMP-9), are critical for basement membrane degradation and are highly upregulated in variety of tumor cells.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the detection of gelatinase activity in biological samples such as recombinant protein, tissue, cell lysates, etcPrincipleThe Gelatinase Activity Assay Kit utilizes a hybrid approach for the detection of gelatinase activity by employing a highly quenched gelatin substrate which upon cleavage by a suitable gelatinase releases a fluorophore, which can be easily quantified using a conventional microplate reader. This method is substrate-specific, simple, fast, high-throughput adaptable and amenable to the sensitive detection of gelatinase activity (as low as 0.06 mCDU).Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS09 hcodes H411 pcodes P273 Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
Glucose (GO) Assay Kit GAGO20-1KT
NACRES: NA.84 ApplicationThis kit is for the quantitative, enzymatic determination of glucose in food and other materials. Glucose is oxidized to gluconic acid and hydrogen peroxide by glucose oxidase. Hydrogen peroxide reacts with o-dianisidine in the presence of peroxidase to form a colored product. Oxidized o-dianisidine reacts with sulfuric acid to form a more stable colored product. The intensity of the pink color measured at 540 nm is proportional to the original glucose concentration.SuitabilitySuitable for the quantitative, enzymatic determination of glucosein food and other materials.Параметрыusage sufficient for 20 assays Quality Level 100 Featured Industry Food and Beverages
General Analyticalstorage temp. 2-8°C
Safety InformationPictograms GHS07,GHS08 Signal Word Danger Hazard Statements H302 - H350 Precautionary Statements P202 - P264 - P270 - P280 - P301 + P312 - P308 + P313 RIDADR NONH for all modes of transport Узнать цену -
Glucose and Sucrose Assay Kit MAK013-1KT
NACRES: NA.84 General descriptionSucrose is a disaccharide that is hydrolyzed to glucose and fructose by invertase. Glucose is the primary carbohydrate utilized for energy during cellular respiration. Upon cellular uptake, glucose can be shunted towards breakdown via glycolysis or polymerized for energy storage.ApplicationGlucose and sucrose assay kit has been used to:- determine sucrose, aerobic glycolysis and pyruvate kinase activity
- determine protein and sugar in strawberries
- determine plasma insulin and glucose levels in mice
- analyze plasma glucose and plasma lactate and to quantify glucose/sucrose.
SuitabilitySuitable for use with various biological samples including serum, plasma, other body fluids, food, and growth media.PrincipleIn this assay kit, glucose is oxidized via glucose oxidase resulting in a colorimetric (570 nm)/ fluorometric (ex = 535 nm/em = 587 nm) product, proportional to the glucose present. To measure sucrose, invertase is added to the reaction to convert the sucrose to glucose and fructose. The free glucose can be subtracted from the total glucose to give the concentration of sucrose present. This kit has a linear range of detection between 0.2-1.0 nmole of glucose for the fluorometric assay and 2-10 nmoles of glucose for the colorimetric assay.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H334 - H412 pcodes P261 - P273 - P284 - P501 Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Glucose Colorimetric/Fluorometric Assay Kit MAK263-1KT
NACRES: NA.84 General descriptionGlucose is a primary energy source that naturally occurs in its free state in fruits and other plant parts. Abnormal glucose levels have been associated with several metabolic dysfunctions such as hypoglycemia, hyperglycemia, and diabetes mellitus. Measurements of glucose levels in tissues and body fluids (such as blood and urine) are often used for the diagnosis of glucose– related disorders. Glucose levels are also monitored to check the efficacy of therapeutics such as insulin and sulphonylureas in type 2 diabetics.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the determination of glucose concentrations in various biological samples, including serum, plasma, food, or growth medium. This kit is also suitable for monitoring glucose levels during fermentation and glucose feeding in protein expression processes.PrincipleGlucose is oxidized to generate a colorimetric (570 nm)/ fluorometric (ex = 535/em = 587 nm) product, proportional to the amount of glucose present. The kit is able to detect 1-10,000 µM of glucose in various samples.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H334 - H412 pcodes P261 - P273 - P284 - P501 RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Glucose Oxidase Activity Assay Kit MAK097-1KT
NACRES: NA.84 General descriptionGlucose Oxidase (GOx, EC 1.1.3.4) is an enzyme found in insects, fungi, and bacteria that catalyzes the oxidation of D-glucose to D-gluconolactone. GOx is widely used in the food, beverage, chemical, and pharmaceutical industries.SuitabilitySuitable for the measurement of glucose oxidase (GOx) in a variety of samples including cells and serumPrincipleThe Glucose Oxidase Activity Assay Kit provides a simple and direct procedure for measuring GOx activity in a variety of biological samples. GOx activity is determined by a coupled enzyme assay, in which GOx oxidizes D-glucose resulting in the production of hydrogen peroxide (H2O2) that reacts with a probe, generating a colorimetric (570 nm)/fluorometric (ex = 535/em = 587 nm) product, proportional to the GOx present. One unit of GOx is defined as the amount of enzyme that generates 1.0 µmole of H2O2 per minute at 37 °C.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 - H412 pcodes P261 - P272 - P273 - P280 - P284 - P302 + P352 RIDADR UN 1993 / PGIII Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Glucose Uptake Colorimetric Assay Kit MAK083-1KT
General descriptionGlucose is the primary source of energy for most cells. Glucose uptake into cells is highly regulated and the first rate limiting step in glucose metabolism. Glucose uptake is facilitated by the GLUT family of transporter proteins, whose expression and activity are regulated by multiple mechanisms. Glucose uptake is upregulated in many cancer cells, which exhibit high rates of aerobic glycolysis. Cells exhibiting insulin resistance show diminished glucose uptake in response to insulin stimulation.
The use of the recycling amplification reaction in the colorimetric assay results in the limit of detection being 10-fold lower (20-100 pmole) compared to the fluorescence assay (200-1,000 pmole, MAK084).ApplicationGlucose uptake colorimetric assay kit has been used to measure glucose uptake by a variety of cells.SuitabilitySuitable for detecting glucose uptake in adherent or suspension cells cultured in a 96-well microtiter plate.PrincipleThe Glucose Uptake Colorimetric Assay kit provides a simple and direct procedure for measuring glucose uptake in a variety of cells. Glucose uptake is measured using the glucose analog, 2-deoxyglucose (2-DG), which is taken up by cells and phosphorylated by hexokinase to 2-DG6P. 2-DG6P cannot be further metabolized and accumulates in cells, directly proportional to the glucose uptake by cells. In this assay, 2-DG uptake is determined by a coupled enzymatic assay in which the 2-DG6P is oxidized, resulting in the generation of NADPH, which is then determined by a recycling amplification reaction in which the NADPH is utilized by glutathione reductase in a coupled enzymatic reaction that produces glutathione. Glutathione reacts with DTNB to product TNB, which is detected at 412 nm.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS09 hcodes H411 pcodes P273 RIDADR NONH for all modes of transport Узнать цену -
Glucose Uptake Fluorometric Assay Kit MAK084-1KT
NACRES: NA.84 General descriptionGlucose is the primary source of energy for most cells.Transport of glucose across the plasma membrane is the first rate limiting step in glucose metabolism. Glucose uptake is facilitated by the GLUT family of transporter proteins, whose expression and activity are regulated by multiple mechanisms. Glucose uptake is upregulated in many cancer cells, which exhibit high rates of aerobic glycolysis. Cells exhibiting insulin resistance show diminished glucose uptake in response to insulin stimulation.ApplicationGlucose Uptake Fluorometric Assay Kit has been used to measure the amount of glucose uptake by monocytic myeloid-derived suppressor cells (M-MDSCs).SuitabilitySuitable for detecting glucose uptake in adherent or suspension cells cultured in a 96-well microtiter plate.PrincipleThe Glucose Uptake Fluorometric Assay kit provides a simple and direct procedure for measuring glucose uptake in a variety of cells. Glucose uptake is measured using the glucose analog, 2-deoxyglucose (2-DG), which is taken up by cells and phosphorylated by hexokinase to 2-DG6P. 2-DG6P cannot be further metabolized and accumulates in cells, directly proportional to the glucose uptake by cells. In this assay, 2-DG uptake is determined by a coupled enzymatic assay in which the 2-DG6P is oxidized, resulting in the generation of NADPH, which reacts with the probe to generate a fluorometric (λex = 535/λem = 587 nm) product, proportional to the 2-DG taken up by the cell.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H315 - H319 - H335 - H412 pcodes P261 - P264 - P271 - P273 - P302 + P352 - P305 + P351 + P338 Target Organs Respiratory system RIDADR UN 1993 / PGIII Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Glucose-1-Phosphate (G1P) Colorimetric Assay Kit MAK098-1KT
NACRES: NA.84 General descriptionGlucose-1-phosphate (G1P), also known as the Cori ester due to its discovery by Gerty and Carl Cori, is produced from glycogen during glycogenolysis by the actions of glycogen phosphorylase. Conversion to glucose-6-phosphate (G6P) by phosphoglucomutase allows for entry of the glucose molecule into metabolic pathways such as glycolysis. During glycogenesis, G6P is converted to G1P by the actions of phosphoglucose isomerase.SuitabilitySuitable for the measurement of glucose-1-phosphate in various tissues (liver, muscle and heart etc.) and cells (adherent or suspension), the analysis of glucose metabolism and cell signaling in various cells and screening anti-diabetic drugs.PrincipleThe G1P Colorimetric Assay kit provides a simple and direct procedure for measuring G1P in a variety of samples. G1P concentration is determined by a coupled enzyme assay, in which G1P is converted to glucose-6-phosphate by phosphoglucomutase in the presence of glucose-1,6-biphosphate. The glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH. The resulting NADH reduces a colorless probe resulting in a colorimetric (450 nm) product proportional to the G1P present.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Glucose-6-Phosphate Assay Kit MAK014-1KT
NACRES: NA.84 General descriptionGlucose 6-phosphate (G6P) is a key metabolic intermediate that enters either metabolic pathways or storage. G6P is generated when glucose is phosphorylated by hexokinase or glucokinase or by the conversion of glucose-1-phosphate by phosphoglucomutase during glycogenolysis. G6P lies at the beginning of both glycolysis and the pentose phosphate pathways. It also can be stored as glycogen when blood glucose levels are high.ApplicationGlucose-6-phosphate assay kit has been used in:- glucose 6-phosphate (G6P) assay
- quantification of glucose-6-phosphate (G6P) and fructose-6-phosphate (F6P)
- the determination of malate
- metabolite analysis
SuitabilitySuitable for quantifying Glucose-6-Phosphate in a variety of samples.PrincipleG6P is utilized by Glucose-6-phosphate dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. G6P is determined by an enzyme assay, which results in a colorimetric (450 nm) product, proportional to the G6P present. Typical sensitivities of detection are between 1-30 nmoles.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
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Glucose-6-Phosphate Dehydrogenase Activity Assay Kit MAK015-1KT
General descriptionGlucose-6-phosphate dehydrogenase (G6PDH) catalyzes the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step of the pentose phosphate pathway (PPP). The PPP pathway critical for maintaining the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH) and for the production of pentose sugars. The NADPH produced is critical for redox regulation via the regeneration of GSH and for providing reducing equivalents for fatty acid biosynthesis. Deficiencies in G6PDH predisposes individuals to non-immune hemolytic anemia.ApplicationGlucose-6-phosphate dehydrogenase assay kit has been used in the determination of glucose-6-phosphate dehydrogenase (G6PDH) activity and to measure PDH (pyruvate dehydrogenase).SuitabilitySuitable for the measurement of Glucose-6-phosphate dehydrogenase (G6PDH) activity in various tissues and cells.PrincipleIn the glucose-6-phosphate dehydrogenase assay kit, glucose-6-phosphate is oxidized to generate a product which is specifically detected by colorimetric (450 nm) assay. The G6PDH Assay Kit can detect G6PDH activity as low as 0.04mU G6PDH per well.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. 20°C Gene Information human ... H6PD(9563)
mouse ... H6PD(100198)
rat ... H6PD(298655)
Safety Informationpictograms GHS05 signalword Danger hcodes H314 pcodes P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 RIDADR NONH for all modes of transport Узнать цену -
Glutamate Assay Kit MAK330-1KT
General descriptionGlutamate is an important chemical in general metabolism. It is also a crucial mammalian neurotransmitter that is believed to be involved in a number of neurological and psychiatric disorders such as lateral sclerosis, lathyrism, autism and Alzheimers disease. Glutamate is also widely used as a flavor enhancer in the food industry. Simple, direct and automation-ready procedures for measuring glutamate concentration are very desirable.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for glutamate determination in serum, plasma, tissue extracts and food extract samples. Also suitable in drug discovery/pharmacology for studying the effects of drugs on glutamate levels.PrincipleThe Glutamate Assay Kit is based on glutamate dehydrogenase catalyzed oxidation of glutamate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the glutamate concentration in the sample. Detection limit of 50 μM , linearity up to 2.5 mM glutamate in 96-well plate assay.Параметрыdetection method colorimetric storage temp. −20°C Узнать цену -
Glutamate Assay Kit MAK004-1KT
NACRES: NA.84 General descriptionGlutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. The synthesis of α-KG from glutamate by glutamate dehydrogenase is a key TCA cycle anaplerotic reaction. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Within the mammalian nervous system, glutamate is the most abundant excitatory neurotransmitter. It is believed to be involved in learning and memory and may also be involved in diseases like amyotrophic lateral sclerosis, autism, some forms of mental retardation, and Alzheimer′s disease. Glutamate is present in a wide variety of foods, and has been used as a flavor enhancer in food industry.ApplicationGlutamate Assay Kit has been used to determine the concentration of glutamate in cerebrospinal fluid in a rat subarachnoid hemorrhage model. It has also been used to determine cytosolic and secreted glutamate concentrations in cerebrospinal fluid of patients with amyotrophic lateral sclerosis.SuitabilitySuitable to determine glutamate concentration in a variety of samples.PrincipleGlutamate concentration is determined by an enzymatic assay, which results in a colorimetric (450 nm) product, proportional to the glutamate present.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS09 hcodes H411 pcodes P273 - P391 - P501 RIDADR NONH for all modes of transport WGK Germany WGK 3 Узнать цену -
Glutamate Dehydrogenase (GDH) Activity Assay Kit MAK099-1KT
General descriptionGlutamate Dehydrogenase (GDH) is a mitochondrial enzyme that catalyzes the reversible oxidative deamination of glutamate to α-ketoglutarate and serves as a key link between anabolic and catabolic pathways. In mammals, GDH is subject to allosteric regulation and has high activity in liver, kidney, brain, and pancreas. GDH activity in serum can be used to differentiate between liver diseases due to liver inflammation, which do not show elevated serum GDH activity, and diseases that result in hepatocyte necrosis, which results in elevated serum GDH.ApplicationGlutamate Dehydrogenase (GDH) Activity Assay Kit has been used to determine the enzymatic activity of glutamate dehydrogenase.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for detecting glutamate dehydrogenase (GDH) activity in serum or tissue and cell extracts.PrincipleGDH activity is determined by a coupled enzyme assay in which glutamate is consumed by GDH generating NADH, which reacts with a probe generating a colorimetric (450 nm) product proportional to the GDH activity present. One unit of GDH is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 7.6 at 37 °C.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. 20°C Gene Information human ... GLUD1(2746), GLUD2(2747)
mouse ... GLUD1(14661)
rat ... GLUD1(24399)
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Glutamate Oxidase Assay Kit MAK170-1KT
EC Number: 200-664-3 NACRES: NA.84 General descriptionGlutamate oxidase is an oxidoreductase that catalyzes the oxidative deamination of L-glutamate to ketoglutarate, ammonia and hydrogen peroxide.
Glutamate oxidase is an oxidoreductase that catalyzes the oxidative deamination of L-glutamate to ketoglutarate, ammonia, and hydrogen peroxide.
The Glutamate Oxidase Assay Kit provides a simple and direct procedure for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts. Glutamate Oxidase activity is determined by a coupled enzyme assay, in which the oxidation of glutamic acid results in a fluorescent product (ex =540/em = 590 nm) proportional to the glutamate oxidase activity present. One unit of glutamate oxidase activity is defined as the amount of enzyme that catalyzes the conversion of L-glutamic acid to 1.0 µmole a-ketoglutaric acid per minute at pH 7.4 at room temperature.SuitabilitySuitable for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts.PrincipleThe glutamate oxidase assay kit provides a simple and direct procedure for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts. Glutamate oxidase activity is determined by a coupled enzyme assay, in which the oxidation of glutamic acid results in a fluorescent product (ex =540/em = 590 nm) proportional to the glutamate oxidase activity present. One unit of glutamate oxidase activity is defined as the amount of enzyme that catalyzes the conversion of L-glutamic acid to 1.0µmole -ketoglutaric acid per minute at pH 7.4 at room temperature.Параметрыusage sufficient for 200 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 pcodes P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312 RIDADR NONH for all modes of transport Узнать цену -
Glutathione Peroxidase Cellular Activity Assay Kit
NACRES: NA.32
Кат. номер CGP1-1KT CGP1-1KT-PW General descriptionThe Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.SuitabilitySuitable for the measureent of glutathione peroxidase in tissue extractsPrincipleThe Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.Other NotesThis kit was tested with rabbit reticulocyte lysate and with glutathione peroxidase enzyme.ПараметрыQuality Level 200, usage sufficient for 100 tests shelf life Kit is stable for 24 months when unopened. detection method colorimetric shipped in wet ice storage temp. −20°C
Safety Informationpictograms GHS02,GHS05,GHS06,GHS08,GHS09 signalword Danger hcodes H226 - H242 - H302 + H332 - H311 - H314 - H341 - H411 pcodes P210 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338 - P370 + P378 RIDADR UN 3316 9 WGK Germany WGK 3 Узнать цену -
Glutathione Reductase Assay Kit
Кат. номер GRSA-1KT GRSA-1KT-PW General descriptionGlutathione reductase (GR) is a ubiquitous enzyme that catalyzes the reduction of oxidized glutathione (GSSG) to glutathione (GSH). Glutathione reductase is essential for the glutathione redox cycle that maintains adequate levels of reduced cellular GSH, which serves as an antioxidant reacting with free radicals and organic peroxides. Glutathione is also a substrate for the glutathione peroxidases and glutathione S-transferases in the detoxification of organic peroxides and the metabolism of xenobiotics.ApplicationGlutathione Reductase Assay Kit has been used to measure the activity of glutathione reductase as a part of oxidative stress assessment and also to study the effects of antifouling biocides on it.Biochem/physiol ActionsGlutathione reductase (EC 1.6.4.2) (GR) is a ubiquitous enzyme that catalyzes the reduction of oxidized glutathione (GSSG) to glutathione (GSH). Glutathione reductase is essential for the glutathione redox cycle that maintains adequate levels of reduced cellular GSH, which serves as an antioxidant reacting with free radicals and organic peroxides. Glutathione is also a substrate for the glutathione peroxidases and glutathione S-transferases in the detoxification of organic peroxides and the metabolism of xenobiotics. This kit contains reagents for the spectrophotometric determination of glutathione reductase activity either by following the decrease in absorbance caused by the oxidation of NADPH at 340 nm (UV assay) or the increase in absorption caused by the reduction of dithiobis(2-nitrobenzoic acid) (DTNB) at 412 nm (colorimetric assay). This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A340 caused by the oxidation of NADPH or the increase in A412 caused by the reduction of dithiobis(2-nitrobenzoic acid).
This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A340 caused by the oxidation of NADPH or the increase in A412 caused by the reduction of dithiobis(2-nitrobenzoic acid).SuitabilitySuitable for the measurement of glutathione reductase activity in biological samplesPrincipleThis kit contains reagents for the spectrophotometric determination of glutathione reductase activity either by following the decrease in absorbance caused by the oxidation of NADPH at 340 nm (UV assay) or the increase in absorption caused by the reduction of dithiobis(2-nitrobenzoic acid) (DTNB) at 412 nm (colorimetric assay). This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A340 caused by the oxidation of NADPH or the increase in A412 caused by the reduction of dithiobis(2-nitrobenzoic acid).Analysis NoteUV assay: One unit will cause the oxidation of 1.0 µmole of NADPH at 25 °C at pH 7.5.
Colorimetric assay: One unit will cause the reduction of 1.0 µmole of DTNB to TNB at 25 °C at pH 7.5.ПараметрыQuality Level 200, usage sufficient for 100 tests detection method colorimetric storage temp. 2-8°C Gene Information human ... GSR(2936)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Glycerol Assay Kit MAK117-1KT
NACRES: NA.84 General descriptionGlycerol, also referred to as glycerin or glycerine, is a 3-carbon sugar alcohol that forms the backbone of fatty acids. It is also a component of phospholipids and triglycerides. Glycerol is a sweet-tasting, hygroscopic liquid. Glycerol is widely used in food, beverage, and pharmaceutical formulations and is the main waste by-product of biodiesel production via transesterification.ApplicationGlycerol assay kit has been used to quantify glycerol release from mouse 3T3-L1 cell culture supernatant samples and to measure plasma glycerol levels.Features and BenefitsCompatible with high-throughput handling systems.SuitabilityThis kit is suitable for glycerol detection in foods, beverages, pharmaceutical formulations, and biological samples.PrincipleIn this kit, glycerol concentration is determined by a coupled enzyme assay involving glycerol kinase and glycerol phosphate oxidase, resulting in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the glycerol present. The linear range of detection for this kit is 10-1,000 μM (colorimetric) and 2-50 μM (fluorometric).Параметрыusage sufficient for 200 colorimetric or fluorometric tests detection method colorimetric, fluorometric shipped in dry ice storage temp. −20°C
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Glycerol-3-Phosphate Assay Kit MAK207-1KT
EC Number: 249-889-9 NACRES: NA.84 General descriptionGlycerol 3-phosphate (G3P) is an important intermediate of carbohydrate and lipid metabolic pathways. It is produced from glycerol by glycerol kinase or from dihydroxyacetone phosphate by glycerol 3-phosphate dehydrogenase. G3P may enter the G3P shuttle to generate NAD+, or may be converted to glyceraldehyde 3-phosphate and enter glycolysis or the lipid biosynthesis pathway.ApplicationGlycerol-3-Phosphate Assay Kit has been used in the determination of glycerol-3-phosphate and phosphoenolpyruvate levels. It has also been used to quantify the concentration in aerial mycelium.SuitabilitySuitable for the detection of Glycerol-3-Phosphate in tissue and cell culture samples.PrincipleThe Glycerol 3-Phosphate Colorimetric Assay Kit provides a simple assay for measuring G3P in various tissues and cells (ranging from 2-10 nmole/well). G3P is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the amount of G3P present.Параметрыdetection method colorimetric storage temp. −20°C
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Glycine Assay Kit (Fluorometric) MAK261-1KT
NACRES: NA.84 General descriptionGlycine (GLY) is a non-essential amino acid used to help create muscle tissue and convert glucose into energy. It′s basic functions include the participation in the synthesis of creatine, glutathione, heme groups, and conjugated bile acids (bile salts). It is also used in the synthesis of DNA and RNA. It is one of the most abundant residues in the triple-helical structure of collagen, which contributes to the elasticity of skin and connective tissue. It acts as a glucogenic amino acid regulating blood sugar levels. Glycine is necessary for central nervous system function. It possesses both inhibitory and excitatory neurotransmitter functions in the brain stem and spinal cord. Recent studies suggest, glycine may play a role in tumorigenesis and malignancy. Some of the external sources of glycine includes legumes, meat, fish and dairy products.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the detection of glycine in biological fluids such as serum, plasma, saliva, urine, etcPrincipleThe Glycine Assay Kit is a simple and reliable, high throughput assay that detects the physiological concentration of glycine in a variety of biological fluids. In the assay, glycine is oxidized generating a fluorometric (lex = 535 nm/ lem = 587 nm) product, proportional to the glycine present. The assay is specific, and other standard and non-standard amino acids do not interfere with the assay. The kit is sensitive to 1 mM of glycine.Параметрыdetection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS05,GHS07 signalword Danger hcodes H290 - H314 - H335 pcodes P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 Target Organs Respiratory system RIDADR UN 3316 9 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Glycogen Assay Kit MAK016-1KT
NACRES: NA.84 General descriptionGlycogen is a branched polymer of glucose that serves as the primary short-term energy storage molecule in animals. Glycogen is primarily synthesized in liver and muscle tissue where it can constitute up to 10% of the weight of liver and 1-2% of the weight of muscle tissue. While muscle glycogen is generally utilized locally, liver glycogen serves as an important buffer to regulate blood glucose levels. Glycogen metabolism is dysregulated in diabetes and the glycogen storage diseases due to inborn errors of metabolism.ApplicationGlycogen Assay Kit has been used in glycogen quantification. It has also been used to determine the glycogen content in liver, vastus lateralis and muscle homogenates.SuitabilitySuitable for determining glycogen concentration in various tissues such as liver etc. and cell culture (adherent or suspension cells).PrincipleGlycogen concentration is determined by a coupled enzyme assay, which produces a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the glycogen present.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) 188.6 °F - closed cup Flash Point(C) 87 °C - closed cup Узнать цену -
Glycogen Phosphorylase Colorimetric Assay Kit MAK417-1KT
General descriptionGlycogen phosphorylase catalyzes the rate-limiting step in glycogenolysis using glycogen and inorganic phosphate to produce glucose-1-phosphate (G1P). In mammals, glycogen phosphorylase is abundant in muscle, liver, and brain tissues. There are two forms of glycogen phosphorylase, namely glycogen phosphorylase a and b forms. Glycogen phosphorylase a is the highly active form whereas glycogen phosphorylase b has only limited activity. Glycogen phosphorylase is a clinically significant enzyme as its mutations are associated with different glycogen storage diseases in muscle and liver. In addition, this enzyme has been suggested as a biomarker for gastric cancer and its inhibition has been tested in treating type 2 diabetes.Features and BenefitsGlycogen Phosphorylase Assay Kit:- Detects as low as 10 mU in a variety of sample types.
SuitabilityThe kit is suitable for the measurement of glycogen phosphorylase activity in cell culture and animal tissues (heart, liver, kidney, muscle, etc.) as well as the analysis of glycogen phosphorylase kinetics, inhibition or activation.PrincipleThe Glycogen Phosphorylase Colorimetric Activity Assay Kit is based on the detection of G1P by a set of enzymatic reactions to generate a colored product with a strong absorbance at A450 nm. The A450 nm signal is directly proportional to the glycogen phosphorylase activity. The kit is a rapid, sensitive and convenient tool for detecting glycogen phosphorylase activity.ПараметрыQuality Level 100, detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05 signalword Danger hcodes H314 - H412 pcodes P273 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 Узнать цену -
Glycolic Acid Assay Kit (Fluorometric) MAK428-1KT
General descriptionGlycolic Acid (GA), also known as Hydroxyl Acetic Acid is the smallest alpha hydroxy acid. Although it is used in several skin care products intended for dermal application in small doses, its acute dose can lead to skin and eye irritation. It is nephrotoxic if swallowed and its toxicity is due to its conversion to oxalic acid, which then precipitates with calcium to form crystals leading to renal tissue injury. GA is also a major downstream metabolite of ethylene glycol metabolism, and a concentration higher than 8 mM in serum indicates the need for hemodialysis. Normally serum GA levels lie in the very low micromolar range (roughly 2 μM).Features and BenefitsGlycolic Acid Assay Kit:- One step plate based assay.
- The kit can quantify as low as 30 pmol of GA.
SuitabilityThe kit is suitable for the measurement of glycolic acid in serum, plasma and urinePrincipleThe Glycolic Acid Assay Kit is a robust, one step plate based assay for the measurement of GA in serum, urine and plasma samples. The GA Detection Reagent metabolizes GA leading to the formation of an intermediate. The intermediate in the presence of GA enzyme mix then converts the non-fluorescent GA probe to a fluorescent product, which is measured at ex = 535 nm/em = 587 nm.ПараметрыQuality Level 100, detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 pcodes P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 Узнать цену -
Glyoxalase I Activity Assay Kit MAK114-1KT
NACRES: NA.84 General descriptionGlyoxalase I (GLO-1), a lactoylglutathione lyase also known as methylglyoxalase, aldoketomutase, ketone-aldehyde mutase, and (R)-S-lactoylglutathione methylglyoxal-lyase, is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methyglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. Glyoxalase I is a target for the development of pharmaceuticals against bacteria, protozoans, and cancer.ApplicationGlyoxalase I Assay Kit has been used to estimate the glyoxalase I activity in plasma samples.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the detection of Glyoxalase I activity in enzyme preparations and biological samples. Suitable for testing the effects of compounds on Glyoxalase I activity.PrincipleThe Glyoxalase I Activity Assay kit provides a simple and direct procedure for measuring GLO-1 activity in a variety of samples such as enzyme preparations or biological samples. In this assay, the GLO-1-mediated production of S-lactoylglutathione is measured by monitoring the change in absorbance at 240 nm. One unit of Glyoxalase I is the amount of enzyme that will convert 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.6 and 25 °C.Параметрыusage sufficient for 100 tests (UV) detection method colorimetric storage temp. 20°C Gene Information human ... GLO1(2739)
mouse ... GLO1(109801)
rat ... GLO1(294320)
Safety Informationpictograms GHS07,GHS08 signalword Warning hcodes H317 - H341 pcodes P201 - P202 - P261 - P280 - P302 + P352 - P308 + P313 RIDADR NONH for all modes of transport WGK Germany WGK 3 Узнать цену -
GPDH Activity Assay Kit MAK208-1KT
NACRES: NA.84 General descriptionGlycerol 3-phosphate dehydrogenase (GPDH; EC 1.1.1.8) catalyzes the reversible conversion of dihydroxyacetone phosphate and NADH to glycerol-3-phosphate and NAD+. It forms a glycerol phosphate shuttle and facilitates the transfer of reduced equivalents across the mitochondria to the cytosol. GPDH is important for both carbohydrate and lipid metabolism. Mutations in GPDH gene result in transient infantile hypertriglyceridemia.ApplicationGPDH (glyceraldhyde-3-phoshate dehydrogenase) activity assay kit has been used to measure the serum and hepatic concentrations of GPDH in rabbit.SuitabilitySuitable for the detection of Glycerol-3-Phosphate Dehydrogenase Activity in tissue and cell culture samples.PrincipleThe Glycerol 3-Phosphate Dehydrogenase Activity Colorimetric Assay Kit provides a simple and sensitive procedure for measuring GPDH activity in a variety of tissues and cells. GPDH activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of GPDH is the amount of enzyme required to generate 1.0 μmole of NADH per minute at pH 8 at 37 °C.Параметрыdetection method colorimetric storage temp. 20°C Gene Information human ... GPD1(2819), GPD2(2820)
mouse ... GPD1(14555), GPD2(14571)
rat ... GPD1(60666), GPD2(25062)
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Granzyme B Activity Assay Kit MAK176-1KT
EC Number: 200-664-3 NACRES: NA.84 General descriptionGZMB codes for granzyme B.
Granzyme B (gzm B) is a peptidase that induces cytotoxic T lymphocyte (CTL)-mediated DNA fragmentation and apoptosis in allogeneic target cells. It cleaves after aspartic acid residues in substrates. Granzyme B-mediated release of mitochondrial cell death factors are required for the activation of caspase-3. Furthermore, the degradation of granzyme B is known to decrease the susceptibility of cancer cells to NK-cell-mediated lysis during hypoxia. Granzyme B activity assays can be used for studying T cell immunity, CTL responses, cell-mediated cytotoxicity, and apoptosis.
Gzm B is a pro-apoptotic member of the granzyme family. It is a neutral serine protease with Aspase activity. Gzm B is expressed exclusively in the granules of activated cytolytic T cells, natural killer cells and lymphokine-activated killer cells.SuitabilitySuitable for the detection of granzyme B activity in biological samples and for the characterization of granzyme B inhibitors.PrincipleGranzyme B activity is determined using an enzyme-catalyzed reaction in which the non-fluorescent substrate, Ac-IEPD-AFC, is hydrolyzed resulting in the release of AFC (λex = 380/λem = 500 nm) proportional to the enzymatic activity present. One unit of granzyme B is the amount of enzyme that hydrolyzes 1 pmole of the substrate per minute at 37 °C.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. 20°C Gene Information human ... GZMB(3002)
mouse ... GZMB(14939)
rat ... GZMB(171528)
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Granzyme B Inhibitor Screening Kit MAK209-1KT
EC Number: 200-664-3 NACRES: NA.25 General descriptionGranzyme B (GZMB; EC 3.4.21.79) is a serine protease stored in the secretory granules of leukocytes. It induces cytotoxic T lymphocyte (CTL)-mediated apoptosis by the activation of caspases and the degradation of structural proteins of the target cells. Granzyme B has an important role in CTL-mediated immune responses and has been implicated in chronic inflammatory diseases such as rheumatoid arthritis, coronary syndrome, and chronic angina pectoris.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the screening of inhibitors of Granzyme B.PrincipleThe Granzyme B Inhibitor Screening Kit is a simple and high-throughput assay for screening potential inhibitors of granzyme B. Granzyme B activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-based substrate to yield AFC, a fluorescent product (ex = 380/ em = 500 nm), proportional to the enzymatic activity present. The substrate includes the recognition sequence for granzyme B.Параметрыdetection method fluorometric storage temp. 20°C Gene Information human ... GZMB(3002)
mouse ... GZMB(14939)
rat ... GZMB(171528)
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HDL and LDL/VLDL Quantitation Kit MAK045-1KT
NACRES: NA.25 General descriptionLipoproteins transport the majority of plasma lipids including cholesterol and triglycerides. High-density lipoprotein (HDL), low-density lipoprotein (LDL), and very-low-density lipoprotein (VLDL) are the lipoproteins responsible for the vast majority of cholesterol transport in the blood. High LDL levels and low HDL levels are strongly associated with increased risk of adverse cardiovascular events.ApplicationHDL and LDL/VLDL Quantitation Kit has been used to determine the concentrations of HDL and LDL/VLDL in serum samples.SuitabilitySuitable for use with plasma and serum samples.PrincipleIn this kit, serum HDL and LDL/VLDL are first separated and then the cholesterol concentration of each is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (ex = 535/em = 587 nm) product, proportional to the cholesterol present. This kit can also be used to determine the concentration of free cholesterol and cholesteryl esters present in a sample.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety InformationRIDADR NA 1993 / PGIII Flash Point(F) 188.6 °F - closed cup Flash Point(C) 87 °C - closed cup Узнать цену -
Heme Assay Kit MAK316-1KT
NACRES: NA.84 General descriptionHeme is a member of the porphyrin family. It is synthesized in both mitochondria and cytoplasm, and is a key prosthetic group for various essential proteins such as hemoglobin, cytochromes, catalases and peroxidases. Free heme, which can be released from hemoglobin following hemolysis, is pro-inflammatory and contributes to iron-derived reactive oxygen species. Heme determination is widely practiced by researchers of various blood diseases.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the quantitative determination of heme and evaluation of drug effects on heme metabolism in a variety of biological samples such as blood, plasma, serum, urine, and heme carrying enzymes.PrincipleHeme assay is based on an aqueous alkaline solution method, in which the heme is converted into a uniform colored form, producing a colorimetric (400 nm) result, directly proportional to the heme concentration in the sample. The optimized formulation reduces interference and exhibits high sensitivity. One mg/dL heme equals 15.3M, 0.001%, or 10ppm. The kit has a linear detection range of 0.6–125M heme in a 96 well format.Параметрыdetection method colorimetric storage temp. 2-8°C
Safety Informationpictograms GHS05,GHS09 signalword Warning hcodes H290 - H319 - H410 pcodes P234 - P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Hemoglobin Assay Kit MAK115-1KT
NACRES: NA.84 General descriptionHemoglobin (Hb) is an iron-containing metalloprotein that serves as the primary means of oxygen transport in vertebrates. Hemoglobin is primarily found in red blood cells where it makes up to 97% of the cell′s dry content. Hemoglobin can also be found in other tissues where it serves as an antioxidant. Alterations in blood hemoglobin levels occurs in many diseases such as anemia and polycytheima.ApplicationHemoglobin Assay Kit has been used to measure the concentration of hemoglobin.Features and BenefitsCompatible with high-throughput handling systems. Can be adapted for use with cuvettes.SuitabilitySuitable for the detection of hemoglobinin a variety of samples such as blood, serum, plasma, and urine. This kit can be used to study the effects of various compounds on hemoglobin metabolism and levels.PrincipleThis assay is based on the improved Triton™[NP1]/NaOH method in which hemoglobin is converted to a colorimetric product measured at 400 nm. This assay has a linear detection range between 0.9-200 mg/dL in the 96 well plate assay.Legal InformationTriton is a trademark of The Dow Chemical Company or an affiliated company of DowПараметрыusage sufficient for 250 colorimetric tests detection method colorimetric storage temp. 2-8°C Gene Information human ... HBA1(3039), HBA2(3040), HBB(3043)
mouse ... HBB(15129)
rat ... HBB(24440)
Safety InformationPictograms GHS05,GHS09 Signal Word Warning Hazard Statements H290 - H319 - H410 Precautionary Statements P234 - P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 RIDADR NONH for all modes of transport WGK Germany WGK 2 Узнать цену -
Hepatic Steatosis Assay Kit MAK258-1KT
NACRES: NA.84 General descriptionHepatic Steatosis, also known as fatty liver, is a disease characterized by an excessive accumulation of lipid droplets within the liver. This can be caused by multiple factors, including excessive alcohol intake, obesity, and other diseases with abnormal fat metabolism. Hepatic Steatosis is commonly used as a marker to determine the hepatotoxicity of drug candidates.SuitabilityEvaluate hepatic steatosis, quantify triglycerides, and visualize lipid droplet accumulation in hepatocytes. As well as screen drug candidates for steatotic risk.PrincipleThe Hepatic Steatosis Kit detects triglyceride accumulation and lipid droplet formation in hepatocytes. The kit can be used to screen and evaluate steatosis risk of drug candidates and to study the mechanism of steatosis development. Chloroquine is included as a positive control for inducing steatosis in liver cells, such as HepG2.Параметрыdetection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS06,GHS08 signalword Danger hcodes H302 - H315 - H317 - H319 - H331 - H335 - H341 - H350 pcodes P280 - P301 + P312 - P302 + P352 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313 Target Organs Respiratory system RIDADR NONH for all modes of transport Узнать цену -
Hexokinase Colorimetric Assay Kit MAK091-1KT
NACRES: NA.84 General descriptionHexokinases, which catalyze the ATP-dependent phosphorylation of aldo- and keto-hexoses to hexose-6-phosphate, catalyze the first step in a number of important biochemical pathways. In mammalian tissues, glucose is the predominant substrate for the hexokinases. There are four isozymes of hexokinase in mammalian tissue, (HK-I, -II, -III, and -IV), which differ in their kinetic and regulatory properties as well as tissue distribution and cofactor use. The hexokinases catalyze the first step in most of the relevant glucose metabolism pathways. Alterations in hexokinase activity are associated with multiple disorders such as X-linked muscular dystrophy, hemolytic anermias, and cancer.ApplicationHexokinase Colorimetric Assay Kit has been used to measure the activity of hexokinase.SuitabilitySuitable for the measurement of Hexokinase activity, analysis of glucose metabolism and cell signaling, and screening anti-diabetic drugs in serum, animal tissues (liver, heart, kidney etc.), and cell culture (adherent or suspension cell).PrincipleThe Hexokinase Colorimetric Assay kit provides a simple and direct procedure for measuring hexokinase activity in a variety of samples. Hexokinase activity is determined by a coupled enzyme assay, in which glucose is converted to glucose-6-phosphate by hexokinase, which is oxidized by glucose-6-phosphate dehydrogenase to form NADH. The resulting NADH reduces a colorless probe resulting in a colorimetric (450 nm) product proportional to the hexokinase activity present. One unit of HK is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at room temperature.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. 20°C Gene Information human ... GCK(2645), HK1(3098), HK2(3099), HK3(3101)
mouse ... GCK(103988), HK1(15275), HK2(15277), HK3(212032)
rat ... GCK(24385), HK1(25058), HK2(25059), HK3(25060)
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
High Sensitivity -Hydroxybutyrate Fluorometric Assay Kit MAK272-1KT
NACRES: NA.25 General descriptionβ -Hydroxybutyrate (β-HB), also known as 3-hydroxybutyrate, is a ketone body primarily synthesized in the liver from the oxidation of fatty acids. Ketone body formation, ketogenesis, is elevated when blood glucose levels drop. Moderately elevated blood ketone bodies occur normally during fasting and prolonged exercise. During alcoholic or diabetic ketoacidosis, blood levels of β -Hydroxybutyrate can rise to abnormally high levels. β -Hydroxybutyrate can be used as a marker for hepatic energy metabolism.SuitabilitySuitable for use with serum, plasma, urine, and other biological fluids, as well as food products such as milk.PrincipleThe High Sensitivity β-Hydroxybutyrate Assay Kit is a simple, sensitive, high-throughput adaptable method for quantifying β-hydroxybutyrate when sample quantities are limited or sub-ketogenic levels are suspected. In this assay, β-hydroxybutyrate is enzymatically oxidized generating a fluorometric signal (lex = 535 nm/lem = 587 nm), proportional to the amount of β-hydroxybutyrate present. The assay is sensitive to 4 mM of β-hydroxybutyrate in a variety of biological samples.Параметрыdetection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H315 - H319 - H335 pcodes P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338 Target Organs Respiratory system RIDADR NONH for all modes of transport Узнать цену -
High Sensitivity Aldehyde Dehydrogenase Activity Fluorometric Assay Kit MAK282-1KT
NACRES: NA.25 General descriptionThe NAD-dependent Aldehyde Dehydrogenase (ALDH) plays a vital role in cellular detoxification. It oxidizes various aldehydes and generates the corresponding carboxylic acid. ALDH have been found in every cellular compartment. Based on its structure and function, ALDH comprises 3 major classes in mammals: Class 1 and Class 3 (the tumor form) are located in the cytosol and include both constitutive and induced forms; Class 2 is located in the mitochondria and only exists as the constitutive form. Aldehyde Dehydrogenase (ALDH) catalyses the oxidation of retinal to retinoic acid, which is essential for growth and development. ALDH is significantly associated with the formation of neurotransmitter, γ-aminobutyric acid (GABA), which is involved in the GABAergic, dopaminergic and opioid system regulation.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the detection of Aldehyde Dehydrogenase activity in a variety of samples.PrincipleThis High Sensitivity Aldehyde Dehydrogenase Activity Assay Kit is a robust tool to quantify ALDH enzymatic activity. In this assay, acetaldehyde is oxidized by ALDH to form NADH, which couples with the High Sensitivity Probe to generate a potent fluorescence ( ex = 535 nm/ em = 587 nm). The ALDH fluorometric assay kit is 10 times more sensitive than the ALDH colorimetric assay and can detect 0.05 mU of ALDH activity in a variety of samples.Параметрыdetection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Узнать цену -
High Sensitivity DHAP Fluorometric Assay Kit MAK275-1KT
NACRES: NA.84 General descriptionDihydroxyacetone phosphate (DHAP) is a metabolic intermediate involved in many pathways, including glycolysis, gluconeogenesis, glycerol metabolism, phosphatidic acid synthesis, fat metabolism and the Calvin cycle. Fructose-1,6-diphosphate is converted to DHAP and glyceraldehyde-3-phosphate (GAP) by aldolase. Both DHAP and GAP serve as the intracellular triose phosphate pool. DHAP can be further converted into GAP by triose phosphate isomerase (TPI). In humans, TPI deficiency is a rare autosomal disease. It causes hemolytic anemia, neurological diseases, and even death due to blockage of the glycolytic pathway and accumulation of DHAP in erythrocytes.
Dihydroxyacetone phosphate (DHAP) is a metabolic intermediate involved in many pathways, including glycolysis, gluconeogenesis, glycerol metabolism, phosphatidic acid synthesis, fat metabolism, and the Calvin cycle. Fructose-1,6-diphosphate is converted to DHAP and glyceraldehyde-3-phosphate (GAP) by aldolase. Both DHAP and GAP serve as the intracellular triose phosphate pool. DHAP can be further converted into GAP by Triose Phosphate Isomerase (TPI). In humans, TPI deficiency is a rare autosomal disease. It causes hemolytic anemia, neurological diseases, and even death due to blockage of the glycolytic pathway and accumulation of DHAP in erythrocytes.SuitabilitySuitable for use with serum, plasma, and other biological fluids, and tissue and cell culture samples.PrincipleThe High Sensitivity Dihydroxyacetone Phosphate Assay Kit is suitable for measuring low levels of DHAP. In this assay, TPI converts DHAP to GAP producing a coupled enzyme reaction, which results in a fluorometric product (lex = 535 nm/lem = 587 nm), proportional to the amount of dihydroxyacetone phosphate present. This assay kit is simple and sensitive with the capability to detect to 0.5 mM of dihydroxyacetone phosphate in a variety of samples.Параметрыdetection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS05,GHS07,GHS09 signalword Danger hcodes H317 - H318 - H411 pcodes P280 - P280 - P305 + P351 + P338 + P310 RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
High Sensitivity Free Glycerol Fluorometric Assay Kit MAK270-1KT
NACRES: NA.25 General descriptionGlycerol, also referred to as glycerin or glycerine, is a 3 carbon sugar alcohol that forms the backbone of fatty acids. It is a central component for synthesis of all lipids, and acts as a backbone for triglycerides and phospholipids, which play an important role in cell membrane structure. Due to its low toxicity, glycerol is widely used in pharmaceutical, food and cosmetic formulations and is the main waste by-product of biodiesel production via transesterificationSuitabilitySuitable for use with serum, plasma, and other biological fluids, and tissue and cell culture samples.PrincipleThe High Sensitivity Free Glycerol Assay Kit is suitable for measuring trace amounts of glycerol in samples, which contain reducing substances that may interfere with oxidase-based assays. This assay employs coupled enzyme reaction, which results in a fluorometric product (lex = 535 nm/lem = 587 nm), proportional to the amount of glycerol present. This assay kit is simple and sensitive with the capability to detect <40 pmole glycerol in a variety of samples.Параметрыdetection method fluorometric storage temp. −20°C
Safety InformationRIDADR UN 3316 9 Flash Point F Not applicable Flash Point C Not applicable Узнать цену
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