Biochemicals
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Covidyte™ ED450 Substrate MAK374-100RXN
General descriptionThe coronavirus main protease, which plays a pivotal role in viral gene expression and replication through the proteolytic processing of replicase polyproteins, is an attractive target for anti-CoV drug design. The inhibition of viral proteases necessary for proteolytic processing of polyproteins has been a successful strategy in the treatment of human immunodeficiency virus (HIV) and hepatitis C respectively, proving the potential of protease inhibitors for the treatment of viral infections. Similarly, the main protease of SARS-CoV-2 is thought to be essential for viral replication and is therefore regarded as a promising target for antiviral therapy of Covid-19.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for developing methods to screen potential inhibitors of coronavirus proteasesPrincipleCovidyte ED450 is a peptide substrate containing an 12-amino acid sequence (VNSTLQSGLRKM) that can be cleaved by coronavirus proteases. The dark-FRET peptide contains DABCYL (quencher) and EDANS (donor) on the N- and C-terminals respectively where the fluorescence of EDANS is effectively quenched by DABCYL when the peptide is intact. When the peptide is hydrolyzed by coronavirus proteases, the EDANS fragment generates significantly enhanced fluorescence since its fluorescence is no longer quenched by DABCYL. The activity of coronavirus proteases can be effectively monitored by the fluorescence intensity of EDANS.Legal InformationCovidyte is a trademark of AAT Bioquest, Inc.Параметрыusage sufficient for 100 fluorometric tests Quality Level 100, detection method fluorometric storage temp. 20°C
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Covidyte™ EN450 Substrate MAK373-100RXN
General descriptionThe coronavirus main protease, which plays a pivotal role in viral gene expression and replication through the proteolytic processing of replicase polyproteins, is an attractive target for anti-CoV drug design. The inhibition of viral proteases necessary for proteolytic processing of polyproteins has been a successful strategy in the treatment of human immunodeficiency virus (HIV) and hepatitis C respectively, proving the potential of protease inhibitors for the treatment of viral infections. Similarly, the main protease of SARS-CoV-2 is thought to be essential for viral replication and is therefore regarded as a promising target for antiviral therapy of Covid-19.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for developing methods to screen potential inhibitors of coronavirus proteasesPrincipleCovidyte EN450 is a peptide substrate containing a 14-amino acid sequence that can be cleaved by coronavirus proteases. The dark-FRET peptide contains Dabcyl (quencher) and Edans (donor) on the N-and C-terminals respectively where the fluorescence of Edans is effectively quenched by Dabcyl when the peptide is intact. When the peptide is hydrolyzed by coronavirus proteases, the Edans fragment generates significantly enhanced fluorescence since its fluorescence is no longer quenched by Dabcyl. The activity of coronavirus proteases can be effectively monitored by the fluorescence intensity of Edans.Legal InformationCovidyte is a trademark of AAT Bioquest, Inc.Параметрыusage sufficient for 100 fluorometric tests Quality Level 100, detection method fluorometric storage temp. 20°C
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Covidyte™ IF670 Substrate MAK376-100RXN
General descriptionThe coronavirus main protease, which plays a pivotal role in viral gene expression and replication through the proteolytic processing of replicase polyproteins, is an attractive target for anti-CoV drug design. The inhibition of viral proteases necessary for proteolytic processing of polyproteins has been a successful strategy in the treatment of human immunodeficiency virus (HIV) and hepatitis C respectively, proving the potential of protease inhibitors for the treatment of viral infections. Similarly, the main protease of SARS-CoV-2 is thought to be essential for viral replication and is therefore regarded as a promising target for antiviral therapy of Covid-19.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for developing methods to screen potential inhibitors of coronavirus proteasesPrincipleCovidyte IF670 is a peptide substrate containing a 12-amino acid sequence (VNSTLQSGLRKM) that can be cleaved by coronavirus proteases. The dark-FRET peptide contains Tide Quencher™ 5 (TQ5) as a quencher and iFluor 670 as a fluorescent donor on the N- and C- terminals respectively where the fluorescence of iFluor 670 is effectively quenched by TQ5 when the peptide is intact. When the peptide is hydrolyzed by coronavirus proteases, the iFluor 670 fragment generates significantly enhanced fluorescence since its fluorescence is no longer quenched by TQ5. The activity of coronavirus proteases can be effectively monitored by the fluorescence intensity of iFluor 670.Legal InformationCovidyte is a trademark of AAT Bioquest, Inc.
Tide Quencher is a trademark of AAT Bioquest, Inc.Параметрыusage sufficient for 100 fluorometric tests Quality Level 100, detection method fluorometric storage temp. 20°C
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Covidyte™ TF670 Substrate MAK375-100RXN
General descriptionThe coronavirus main protease, which plays a pivotal role in viral gene expression and replication through the proteolytic processing of replicase polyproteins, is an attractive target for anti-CoV drug design. The inhibition of viral proteases necessary for proteolytic processing of polyproteins has been a successful strategy in the treatment of human immunodeficiency virus (HIV) and hepatitis C respectively, proving the potential of protease inhibitors for the treatment of viral infections. Similarly, the main protease of SARS-CoV-2 is thought to be essential for viral replication and is therefore regarded as a promising target for antiviral therapy of Covid-19.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for developing methods to screen potential inhibitors of coronavirus proteasesPrincipleCovidyte TF670 is a peptide substrate containing a 14-amino acid sequence that can be cleaved by coronavirus proteases. The dark-FRET peptide contains Tide Quencher™ 5 (TQ5) as a quencher and Tide Fluor™ 5 (TF5) as a fluorescent donor on the N- and C-terminals respectively where the fluorescence of TF5 is effectively quenched by TQ5 when the peptide is intact. When the peptide is hydrolyzed by coronavirus proteases, the TF5 fragment generates significantly enhanced fluorescence since its fluorescence is no longer quenched by TQ5. The activity of coronavirus proteases can be effectively monitored by the fluorescence intensity of TF5.Legal InformationCovidyte is a trademark of AAT Bioquest, Inc.
Tide Fluor is a trademark of AAT Bioquest, Inc.
Tide Quencher is a trademark of AAT Bioquest, Inc.Параметрыusage sufficient for 100 fluorometric tests Quality Level 100, detection method fluorometric storage temp. 20°C
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
COX Activity Assay Kit (Fluorometric) MAK414-1KT
General descriptionCyclooxygenase (COX), also known as prostaglandin-endoperoxide synthase (PTGS), is an enzyme that is responsible for the formation of important biological mediators called prostanoids, including prostaglandins, prostacyclin and thromboxane. COX is the central enzyme in the biosynthetic pathway to prostanoids from arachidonic acid. There are two known isoenzymes: COX-1 and COX-2. COX-1 is constitutively expressed in many tissues and is the predominant form in gastric mucosa and in kidney. COX-2 is not expressed under normal conditions in most cells, but elevated levels are observed during inflammation. Pharmacological inhibition of COX by non-steroidal anti-inflammatory drugs (NSAID) can provide relief from the symptoms of inflammation and pain.Features and BenefitsCOX Activity Assay Kit:- Compatible with high-throughput handling systems.
- Includes COX-1 inhibitor (SC560) & COX-2 Inhibitor (Celecoxib).
SuitabilityThe kit is suitable for the measurement of COX activity in various biological samples (such as adherent and suspension cells and tissues) and purified/crude enzyme preparations.PrincipleThe COX Activity Assay Kit provides a simple method to detect the peroxidase activity of COX in biological samples or purified/crude enzyme preparations. The kit includes COX-1 and COX-2 specific inhibitors to differentiate the activity of COX-1 and COX-2, as well as other peroxidases, which may be present in the sample.ПараметрыQuality Level 100, detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H302 + H312 + H332 - H315 - H319 - H335 pcodes P261 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 Target Organs Respiratory system Узнать цену -
COX-2 Inhibitor screening kit MAK399-1KT
General descriptionCyclooxygenase (COX), also known as prostaglandin-endoperoxide synthase (PTGS), is an enzyme that is responsible for the formation of important biological mediators called prostanoids, including prostaglandins, prostacyclin and thromboxane. COX is the central enzyme in the biosynthetic pathway to prostanoids from arachidonic acid. There are two known isoenzymes: COX-1 and COX-2. COX-1 is constitutively expressed in many tissues and is the predominant form in gastric mucosa and in kidney. COX-2 is not expressed under normal conditions in most cells, but elevated levels are found during inflammation. Pharmacological inhibition of COX by non-steroidal anti-inflammatory drugs (NSAID) can provide relief from the symptoms of inflammation and pain.Features and BenefitsCOX-2 Inhibitor Screening Kit:- Compatible with high-throughput handling systems.
SuitabilityThe kit is suitable for screening, studying and characterizing COX-2 inhibitors.PrincipleThe assay is based on the fluorometric detection of Prostaglandin G2, the intermediate product generated by the COX enzyme.ПараметрыQuality Level 100, detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H302 + H312 + H332 - H315 - H319 - H335 pcodes P261 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 Target Organs Respiratory system Узнать цену -
Creatine Assay Kit MAK079-1KT
NACRES: NA.84 General descriptionCreatine is a nitrogenous compound that acts as a high-energy reservoir for the rapid regeneration of ATP. Approximately 95% of creatine is found in skeletal muscle, primarily as phosphocreatine. Creatine can be acquired through dietary consumption or formed from L-arginine, glycine, and L-methionine in a multi-step reaction that occurs in the kidneys and liver. Creatine is then transported to muscle tissue. Creatine supplementation is used for the enhancement of sports performance, primarily by increasing muscle mass. Creatine is also being investigated as a treatment of neuromuscular diseases, where it may aid in neuroprotection and by improving the cellular bioenergetic state.ApplicationCreatine Assay Kit has been used to analyze the creatine content in samples.SuitabilitySuitable for the measurement of creatinine in biological fluids including serum, urine, CSF etc.PrincipleIn this assay, Creatine concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535em/λ = 587 nm) product, proportional to the creatine present.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C Узнать цену -
Creatine Kinase Activity Assay Kit MAK116-1KT
NACRES: NA.84 General descriptionCreatine Kinase (CK), also known as phosphocreatine kinase, is an enzyme that catalyzes the transfer of one phosphate group from ATP to creatine generating phosphocreatine, an important energy reservoir in muscle and brain tissue. CK is a dimeric protein made up of B (brain) and M (muscle) subunits. Three isoenzymes, CK-MM, CK-MB, and CK-BB, have been observed. CK levels are elevated in various pathological conditions including myocardial infarction, rhabdomyolysis, muscular dystrophy, and renal failure.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the detection of Creatine Kinase activity in a variety of samples such as blood, serum, and plasma. This kit can be used to study the effects of various compounds on Creatine Kinase activity.PrincipleCreatine Kinase activity is determined by a coupled enzyme reaction resulting in the production of NADPH, measured at 340 nm, proportionate to the CK activity present in the sample. In this reaction, phosphocreatine and ADP are converted to creatine and ATP. The generated ATP is used by hexokinase to phosphorylate glucose resulting in glucose-6-phosphate, which is oxidized by NADP in the presence of glucose-6-phosphate dehydrogenase to produce NADPH and 6-phospho-D-gluconate. One unit of CK is the amount of enzyme that will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per minute at pH 6.0. This kit has a linear range of 30–1,800 units/L CK activity.Параметрыusage sufficient for 100 tests (Ultraviolet) detection method colorimetric shipped in dry ice storage temp. 20°C Gene Information human ... CKB(1152), CKM(1158)
mouse ... CKB(12709), CKM(12715)
rat ... CKB(24264), CKM(24265)
Safety Informationpictograms GHS08 signalword Danger hcodes H334 pcodes P261 - P284 - P304 + P340 + P312 - P501 RIDADR NONH for all modes of transport Узнать цену -
Creatinine Assay Kit MAK080-1KT
NACRES: NA.84 General descriptionCreatinine is generated from creatine by nonenzymatic dehydration. Creatinine is produced at a constant rate and is excreted from the body through kidney glomerular filtration. Decreased kidney function can effect the rate at which creatinine is filtered by the kidneys and can be used as a measure of kidney function. Decreased kidney function can result in increased serum creatinine levels due to the inability to clear creatinine through urine excretion. Creatinine levels can be affected by changes in muscle mass, pregnancy, or the use of angiotensin inhibitors or angiotensin receptor antagonists.ApplicationCreatinine Assay Kit has been used to measure the creatinine concentration in samples.SuitabilitySuitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.PrincipleIn this assay, Creatinine concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535em/λ = 587 nm) product, proportional to the creatinine present.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H334 - H412 pcodes P261 - P273 - P284 - P501 RIDADR NA 1993 / PGIII Узнать цену -
Cysteine Assay Kit (Fluorometric) MAK255-1KT
NACRES: NA.84 General descriptionCysteine (CYS) is a non-essential, sulfur containing amino acid in humans, related to cystine. Cysteine is important for protein synthesis, detoxification, and diverse metabolic functions. It is found in beta-keratin, which comprises the main protein in nails, skin, and hair. Cysteine is also important in collagen production, as well as skin elasticity and texture. A component of the antioxidant, glutathione, cysteine plays a role in the metabolism of essential biochemicals such as coenzyme A, heparin, and biotin. An elevated level of total cysteine can predict cardiovascular disease and metabolic syndromes. Cysteine deficiency is known to be one of the consequences of aging.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the detection of cysteine in serum, plasma, and other biological fluids.PrincipleThe Cysteine Assay Kit detects the physiological concentration of cysteine in a variety of biological fluids. The assay is based on the cleavage of thiol group of reduced cysteine generating a fluorometric product (ex = 365 nm/ em = 450 nm), directly proportional to the amount of total cysteine in the sample. The assay is specific and other thiol-based amino acids do not interfere with the assay. This high-throughput adaptable assay kit is simple and sensitive enough to detect to 10 M of cysteine in a variety of samples.Параметрыdetection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
D-2-Hydroxyglutarate (D2HG) Assay Kit
Кат. номер MAK320-1KT MAK320-1KT-PW General descriptionThe level of D-2-Hydroxyglutarate (D2HG) is low in normal cells and tissues, but is significantly elevated in metabolic diseases, such as the rare autosomal disorder D2HG aciduria. D2HG is mildly elevated in other metabolic disorders including multiple acyl-CoA dehydrogenase deficiency, dihydrolipoyl dehydrogenase deficiency, pyruvate decarboxylase deficiency and pyruvate carboxylase deficiency, various cancers, and in neoplasms with mutations in the isocitrate dehydrogenase 1 (IDH1) and isocitrate dehydrogenase 2 (IDH2) genes. Detection of elevated D2HG is an important biomarker for early diagnosis, prognosis, monitoring and the development of therapeutic strategies against these diseases.Features and Benefits- Highly sensitive and rapid enzymatic assay for the detection of D2HG levels in various biological fluids
- Quick instruction bench card - to assure your experimental success
- More experiments in one kit - contains sufficient reagents for 200 tests
- Detection time: only 30-60 minutes
SuitabilitySuitable for the detection of D2HG levels in cells and tissues lysates, serum, urine, cultured cells and culture supernatants.PrincipleDeveloped in partnership with the German Cancer Research Center (DKFZ), the D2HG Assay Kit is a rapid and sensitive enzymatic assay for the detection of D2HG levels in various biological fluids: serum, urine, cell culture supernatants, and cell or tissue lysates. The assay, originally developed by Balss et al., 8 is based on the oxidation of D2HG to -ketoglutarate (KG) by the enzyme (D)-2-hydroxyglutarate dehydrogenase (HGDH) coupled to the reduction of NAD+ to NADH (see Figure 1). The amount of NADH formed is then quantitated by the diaphorase mediated reduction of resazurin to the fluorescent dye resorufin (ex = 540 nm/em = 590 nm).Legal InformationThis product is sold under license from the German Cancer Research Center (DKFZ) and University Clinic of Heidelberg. Use of this product is covered by certain US and foreign patents, including US Pat. No. 9,487,815, EP Pat. No. 2 820 145 and foreign equivalents. Use of this product is for research purposes only.Параметрыusage sufficient for 200 reactions (Fluorometric) detection method fluorometric shipped in wet ice storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
D-Gluconate (D-Gluconic Acid) Assay Kit (Colorimetric) MAK279-1KT
NACRES: NA.84 General descriptionD-Gluconic acid (C6H12O7) is a mild organic acid that occurs from the oxidation of glucose. It is naturally present in fruit, honey, and wine When used as a food additive, it helps regulate acidity. It is a strong chelating agent, chelating the anions of calcium, iron, aluminum, copper, and other heavy metals. D-Gluconate (C6H11O7) is the salt or ester of Gluconic Acid. Due to its low toxicity, it is widely used in pharmaceutical, food, and other industries.SuitabilitySuitable for the measurement of D-Gluconate (D-Gluconic Acid) in various samples such as tissues, plants, fruits and winePrincipleThe D-Gluconate (gluconic acid) Assay Kit is a simple and sensitive method for quantifying D-gluconate. In this assay, gluconate is utilized by glucokinase to form D-gluconate-6-phosphate and ADP producing a coupled enzyme reaction that generates a colorimetric signal (450 nm), proportional to the amount of D-gluconate present. The assay is sensitive to less than 2 M of D-gluconate in a variety of samples.Параметрыdetection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05,GHS08 signalword Danger hcodes H314 - H334 pcodes P261 - P280 - P284 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 RIDADR NONH for all modes of transport Узнать цену -
D-Lactate Assay Kit MAK336-1KT
General descriptionLactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for D-lactate in serum, plasma, and cell media samples.PrincipleThe D-Lactate Assay Kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C Узнать цену -
D-Lactate Colorimetric Assay MAK058-1KT
NACRES: NA.84 General descriptionD-Lactate, typically present in the bloodstream at nanomolar concentrations, is produced by an intestinal source or via the methylglyoxal pathway. In mammals, D-Lactate metabolism requires D-Lactate hydrogenase and is metabolized slowly, thus an increase in blood concentration levels can lead to acidemia and acidosis. The severity of this D-lactic acidosis can be associated with neurotoxic symptoms. Significant D-Lactate accumulations in the body can also be related to impaired metabolism and excretion.ApplicationD-Lactate Colorimetric Assay kit has been used to determine the stereospecificity of lactate produced.SuitabilitySuitable for use with samples of serum, plasma, cells, culture and fermentation media.PrincipleIn this assay, D-Lactate is specifically oxidized by D-Lactate hydrogenase and generates a proportional colorimetric product measured at 450 nm. The useful concentration range in samples is 0.1-10 mM D-Lactate.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. 20°C
Safety Informationpictograms GHS05 signalword Danger hcodes H318 - H412 pcodes P273 - P280 - P305 + P351 + P338 RIDADR NONH for all modes of transport Узнать цену -
D-Mannitol Colorimetric Assay Kit MAK096-1KT
NACRES: NA.84 General descriptionD-Mannitol is a common sugar alcohol (polyol) produced by many organisms including bacteria, fungi, and plants. D-Mannitol serves as a carbon storage compound, an osmoregulating agent, and antioxidant by protecting against reactive oxidative species (ROS). Mannitol is commonly used in food manufacturing as a reduced-calorie sweetener. The sugar alcohol L-arabitol also acts as a substrate for this assay; therefore, this kit can be used to measure L-arabitol.ApplicationD-Mannitol Colorimetric Assay Kit has been used to assess the intracellular mannitol content in samples.SuitabilitySuitable for the detection of D-Mannitol/L-Arabitol in plant tissues, fungi, bacteria, food, juices, other beverages, etc.PrincipleThe D-Mannitol Colorimetric Assay kit provides a simple and direct procedure for measuring D-mannitol in a variety of samples, including fruit and juices. D-Mannitol concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product proportional to the D-mannitol present.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
D-Sorbitol Colorimetric Assay Kit MAK010-1KT
NACRES: NA.84 General descriptionD-Sorbitol is a sugar alcohol that is commonly used as a sugar substitute. It occurs naturally and is also produced synthetically from glucose. The food industry uses D-sorbitol as an additive in the form of a sweetener, humectant, emulsifier, thickener, or dietary supplement. D-Sorbitol has also been found in cosmetics, paper, and pharmaceuticals. Naturally, D-sorbitol occurs widely in plants via photosynthesis, ranging from algae to higher order fruits of the family Rosaceae.SuitabilitySuitable to measure D-sorbitol levels in samples such as foods, fruits, fruit juices, cosmetics, pharmaceuticals, and paper. The assay is NOT recommended for plasma, serum, or urine samples.PrincipleIn this assay, D-sorbitol is oxidized to fructose with the proportional development of intense color with an absorbance maximum at 560 nm. The assay is useful in the range of 0.1-10 nmoles of D-sorbitol per sample.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Deproteinizing Sample Preparation Kit MAK341-1KT
General descriptionThe presence of protein and various enzyme activities frequently interferes with the analysis of small molecules in biological samples. Many bioassays require removal of proteins from samples prior to analysis. Among the deproteinization protocols developed over the last half century, perchloric acid (PCA) precipitation has been extensively used in many different sample preparation procedures, since not only does it remove most of the protein present but it also functions to stabilize many of the small molecule analytes. PCA deproteinization methods have been successfully used in the preparation samples prior to quantitation of an array of small molecules, including glycogen, ATP, cAMP, glutathione, antioxidants, etc.SuitabilitySuitable for deproteinizing various biological samplesPrincipleThe Deproteinizing Sample Preparation Kit utilizes a PCA precipitation method, providing a unique tool to prepare samples for the analysis of a variety of small moleculesПараметрыusage sufficient for 200 assays
Safety Informationpictograms GHS03,GHS05,GHS07,GHS08 signalword Danger hcodes H272 - H290 - H302 + H332 - H314 - H373 pcodes P210 - P280 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338 + P310 - P314 Target Organs Thyroid Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Deproteinizing Sample Preparation Kit II MAK342-1KT
General descriptionThe study of biological fluids, cell, bacteria, yeast, and tissue lysates has delivered vital information regarding their biochemical composition. These biological samples comprise a rich, complex list of lipids, proteins, small organic molecules, ions, and, depending on the sample, exogenous molecules (i.e. drugs). Several small organic molecules can also be found free and/or bound to other macromolecules. These macromolecules (lipoproteins, proteins, enzymes) interfere or degrade low molecular-weight metabolites. Therefore, sample deproteinization is often required. Chemical deproteinization procedure such as Trichloroacetic acid (TCA) is one of the most utilized methods to accomplish sample deproteinization.SuitabilitySuitable for deproteinizing biological fluids (serum, plasma, whole blood, saliva, urine etc.) and lysates (cell culture, mammalian tissue, yeast, bacteria etc.)PrincipleThe Deproteinizing Sample Preparation Kit II offers an excellent alternative when metabolite lability affects the accurate detection and estimation of small molecule concentrations and when organic solvents or ultrafiltration cannot be used. TCA precipitates proteins by lowering the sample pH drastically. After removal of precipitated proteins, the pH of the sample is neutralized with neutralization buffer that is provided in the kit.Параметрыusage sufficient for 200 assays
Safety Informationpictograms GHS05,GHS07,GHS09 signalword Danger hcodes H290 - H302 + H332 - H314 - H335 - H410 pcodes P273 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338 + P310 Target Organs Respiratory system Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Diamine Oxidase Activity Assay Kit (Fluorometric) MAK351-1KT
General descriptionDiamine Oxidase (DAO) is an important regulator of polyamine levels in human physiology. DAO metabolizes the oxidative conversion of biogenic polyamines such as putrescine and cadaverine into aldehydes. Biogenic amines are known regulators of important biological pathways such as cell growth and signal transduction, placing DAO in an important regulatory position in maintaining cell viability. Putrescine itself is also an activator of c-jun and c-fos expression, further linking this diamine with potential roles in cancer development. DAO is notably involved in regulating growth and differentiation of intestinal mucosa, bone marrow, and other rapidly proliferating tissue. In addition to its role in tissue growth, DAO activity is important in metabolism. Loss of diamine oxidase activity can lead to histamine intolerance and histamine poisoning.SuitabilitySuitable for the measurement of DAO activity in various tissues and cell extracts as well as the characterization of DAO activity of purified recombinant enzyme.PrincipleThe Diamine Oxidase Activity Assay Kit provides a straightforward method to determine DAO activity of tissue lysates as well as recombinant enzymes, with a detection limit of less than 1 pmole/minute of activity. In the assay, DAO converts the provided substrate, yielding an intermediate and H2O2. H2O2 is then utilized by the DAO Enzyme Mix to generate fluorescence (Ex/Em = 535/587 nm) from the DAO Probe.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS05,GHS06 signalword Danger hcodes H290 - H302 - H311 + H331 - H314 - H412 pcodes P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
DL-Serine Assay Kit MAK352-1KT
General descriptionSerine is one of the 20 naturally-occurring amino acids used by all organisms in the biosynthesis of proteins. Having a single chiral center, serine can exist as either of two stereoisomers (L-Serine and D-Serine). In addition to its role as a protein building block, L-Serine serves as a precursor to many vital biomolecules, including phosphatidylserine, sphingomyelin and the amino acids glycine and cysteine. The non-proteogenic isomer D-Serine is also synthesized from L-Serine in the mammalian brain. While most D-amino acids are only present in trace amounts in mammals, D-Serine is a vital neurotransmitter that acts as a co-agonist of synaptic N-methyl-D-aspartate (NMDA) type glutamate-gated ion channels. Impaired D-Serine metabolism may contribute to several neuropsychiatric disorders, including depression, schizophrenia, epilepsy and dementia.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the Estimation of D- and L-Serine concentrations in human or animal biological fluids (plasma, serum, CSF, etc.), soft tissue homogenates (i.e. liver, brain, etc.) and cultured cell lysates (adherent or suspension cells).PrincipleThe DL-Serine Assay Kit allows for quantification of both L- and D-Serine in biological fluids and tissues. The assay is based on the conversion of L-Serine to D-Serine, which is metabolized to an intermediate product that is subsequently oxidized and reacts with a probe to form a stable fluorophore (Ex/Em = 535/587 nm). Samples may be divided and assayed simultaneously for quantification of both D-Serine and total DL-Serine. The assay is not affected by physiological concentrations of other amino acids, is high-throughput adaptable and can detect less than 1 μM Serine.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety InformationFlash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
DNAse I Activity Assay Kit (Fluorometric) MAK397-1KT
General descriptionDeoxyribonuclease I (DNAse I) is an endonuclease that cleaves DNA phosphodiester bonds yielding 5′-phosphorylated and 3′-hydroxylated oligonucleotides. DNAse I targets single-stranded DNA, double-stranded DNA, and chromatin in a non-specific manner. As an important player in cellular waste management, DNAse I is normally secreted extracellularly to clear the system from circulating cell-free DNA, foreign DNA from food digestion or potential pathogens, and endogenous chromosomal DNA from apoptotic and necrotic cells. Abnormal DNAse I activity occurs in association with a variety of cancers and auto-immune illnesses that exhibit elevated levels of cell-free DNA. Furthermore, DNAse I has been therapeutically used in cystic fibrosis patients to degrade DNA and reduce sputum viscosity.Features and BenefitsDNAse I Activity Assay Kit:- The limit of quantification is 178 fmoles of DNA probe cleaved per minute per mL.
SuitabilityThe kit is suitable for the Measurement of DNAse I activity of purified proteins, the quantitative analysis of DNAse I mutants and inhibitors, and the comparative examination of DNAse I activity in serum and other biological samples.PrincipleThe DNAse I Activity Assay Kit allows for quantitative evaluation of DNAse I activity of purified enzymes and their inhibitors, as well as comparative examination of DNAse I activity in biological samples. Enzyme activity is detected upon cleavage of a DNA Probe, which yields a fluorescent DNA product measured at ex = 651nm/em = 681nm. The limit of quantification (LOQ) is 178 fmoles of DNA probe cleaved per minute per mL.ПараметрыQuality Level 100, detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H334 pcodes P261 - P284 - P304 + P340 + P312 - P501 Узнать цену -
DPP4 Activity Assay Kit MAK088-1KT
EC Number: 200-664-3 NACRES: NA.84 General descriptionDipeptidyl peptidase-4 (DPP4), also known as CD26 and adenosine deaminase complexing protein 2, is a serine exopeptidase that cleaves X-Proline and X-Alanine residues from the N-termini of polypeptides. DPP4 is a transmembrane glycoprotein whose activity regulates the bioactivity of multiple peptides such as growth factors, chemokines, and neuropeptides. DPP4 plays a major role in glucose metabolism via the regulation of glucagon-like peptide-1 and inhibitors of DPP4 are commonly used for the treatment of type 2 diabetes. DPP4 also plays an important role in immune regulation and may play a role in tumor suppression. Deletion of the DPP4 gene affects carbohydrate metabolism which in turn leads to mental retardation and hypotonia. Abnormal expression of the protein is observed during liver regeneration, cirrhosis and liver tumorigenesis.
ApplicationDPP4 Activity Assay Kit has been used to determine the activity of dipeptidyl peptidase-4 (DPP4) in homogenates.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the measurement of dipeptidyl peptidase-4 (DPP4) in a variety of samples including tissue, cells and serumPrincipleThe DPP4 Activity Assay kit provides a simple and direct procedure for measuring DPP4 activity in a variety of samples. In this assay, DPP4 cleaves a non-fluorescent substrate, H-Gly-Pro-AMC, to release a fluorescent product, 7-Amino-4-Methyl Coumarin (AMC) (ex = 360/em = 460 nm). One unit of DPP4 is the amount of enzyme that will hydrolyze the DPP4 substrate to yield 1.0 µmole of AMC per minute at 37 °C.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. 20°C Gene Information human ... DPP4(1803)
mouse ... DPP4(13482)
rat ... DPP4(25253)
Safety InformationRIDADR UN 1993 / PGIII Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
DPP4 INHIBITOR SCREENING KIT MAK203-1KT
NACRES: NA.25 General descriptionDipeptidyl-peptidase 4 (DPP4; CD26; adenosine deaminase complexing protein-2) is a membrane glycoprotein with serine exopeptidase activity that cleaves X-proline dipeptides from the N-terminus of polypeptides. Inhibitors of DPP4 inhibit the degradation of glucose-dependent insulinotropic polypeptide and glucagon-like peptide-1 by DPP4 and have emerged as oral anti-diabetic agents.SuitabilitySuitable for the screening of inhibitors of DPP4.PrincipleThe DPP4 Inhibitor Screening Kit is a simple assay suitable for high-throughput screening of potential DPP4 inhibitors. DPP4 activity is measured by cleaving the substrate to yield a fluorescent product (ex = 360/em = 460 nm), proportional to the enzymatic activity present. The effectiveness of the test inhibitors may be compared with the DPP4 inhibitor (sitagliptin) provided as a control.Параметрыdetection method fluorometric shipped in wet ice storage temp. 2-8°C Gene Information human ... DPP4(1803)
mouse ... DPP4(13482)
rat ... DPP4(25253)
Safety InformationRIDADR NONH for all modes of transport Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Enolase Activity Assay Kit MAK178-1KT
NACRES: NA.84 General descriptionEnolase is a multifunctional glycolytic enzyme that catalyzes the conversion of D-2-phosphoglycerate to phospho(enol)pyruvate (PEP) and water. It can function as a plasminogen receptor in endothelial, epithelial, and hematopoietic cells and hence, may be involved in fibrinolytic and intravascular systems. It is also known to act as a heat shock protein, which may have implications in transcriptional and pathological functions. Enolase has been implicated in autoimmune and systemic diseases. Furthermore; serum neuron-specific enolase is known to function as a predictor of patient outcome post cardiac arrest. Thus enolase assays can be used for studying cellular functions like carbohydrate metabolism, transcription and other pathophysiological processes.ApplicationEnolase Activity Assay Kit has been used to measure enolase activity.SuitabilitySuitable for the detection of enolase activity in biological samples.PrincipleThe Enolase activity is determined by a coupled enzyme assay in which D-2-phosphoglycerate is converted to PEP, resulting in the formation of an intermediate that reacts with a peroxidase substrate, generating a colorimetric (570 nm) or fluorometric (ex = 535/em = 587 nm) product proportional to the enolase activity present. One milliunit of enolase is the amount of enzyme that will generate 1.0 nmole of H2O2 per minute at pH 7.2 at 25 °C.Параметрыdetection method colorimetric, fluorometric storage temp. 20°C Gene Information human ... EBI4(387712), ENO1(2023), ENO2(2026)
mouse ... EBI4(226265), ENO1(13806), ENO2(13807)
rat ... EBI4(292138), ENO1(24333), ENO2(24334)
Safety Informationpictograms GHS03,GHS05,GHS07 signalword Danger hcodes H271 - H302 + H332 - H314 - H335 - H412 pcodes P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 Target Organs Respiratory system RIDADR NONH for all modes of transport Узнать цену -
Enteropeptidase Activity Assay Kit MAK204-1KT
EC Number: 200-664-3 NACRES: NA.84 General descriptionEnteropeptidase (Enterokinase; EC 3.4.21.9) is an enzyme that converts trypsinogen to trypsin, the activity of which is required for the activation of chymotrypsin and procarboxypeptidases. It has an important role in food digestion and may serve as a marker of differentiated enterocytes and goblet cells.SuitabilitySuitable for the measurement of enteropeptidase/enterokinase activity in biological samples or purified preparations.PrincipleThe Enteropeptidase/Enterokinase Activity Assay Kit is a rapid method to measure enteropeptidase activity in biological samples. Enteropeptidase activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-tagged peptide substrate containing the recognition sequence for enteropeptidase. This yields AFC, a fluorescent product (λex = 380/ λem = 500 nm), proportional to the enzymatic activity present. One unit of enteropeptidase is the amount of enzyme that generates 1.0 nmole of AFC per minute at room temperature.Параметрыdetection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Ethanol Assay Kit MAK076-1KT
NACRES: NA.84 General descriptionEthanol is a psychoactive component of many commonly consumed drinks where it acts as a central nervous system depressant. After ingestion, ethanol is absorbed into the bloodstream via the stomach and small intestine. Ethanol is largely metabolized by the liver but is also secreted in urine or through respiration. The monitoring of ethanol levels is also important in fermentation processes. The ethanol assay kit provides a simple and reliable method for the quantification of ethanol.
This kit is highly sensitive to the presence of short-chain alcohols (ethanol, methanol, and propanol). Storage or use of this kit in the vicinity of alcohol vapors can result in the uptake of the alcohols by kit components, resulting in very high backgrounds.ApplicationEthanol Assay Kit has been used :- to measure the levels of ethanol in yeast cell culture supernatant and to study the link between metabolism and chromatin structure
- to determine the ethanol levels in compound beverages and foods
- to measure ethanol content in vinegar
SuitabilitySuitable for determining the concentration of ethanol in serum, plasma, and other body fluids as well as in beverages and growth media.PrincipleEthanol concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (ex = 535/ex = 587 nm) product, proportional to the ethanol present. The Ethanol Assay Kit provides a simple and reliable method for the quantification of ethanol in serum, plasma, and other body fluids as well as in beverages and growth media.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS02,GHS07 signalword Danger hcodes H225 - H319 pcodes P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338 RIDADR UN 3316 9 Flash Point F 57.2 °F - closed cup Flash Point C 14.0 °C - closed cup Узнать цену -
Factor IXa Activity Assay Kit (Fluorometric) MAK240-1KT
NACRES: NA.84 General descriptionThe coagulation Factor IX (or Christmas factor) (EC 3.4.21.22) is a vitamin K-dependent serine protease. Factor IX is produced as an inactive precursor and is activated via cleavage by either factor XIa (contact pathway) or factor VIIa (tissue factor pathway). In the presence of calcium ions and negatively charged membrane phospholipids, activated factor IX (FIXa) then binds to the activated Factor VIII (FVIIIa) and proteolytically activates factor X (FX) to factor Xa (FXa). Deficiency in factor IX causes hemophilia B, an X-linked bleeding disorder.SuitabilitySuitable to determine the activity of purified Factor IX and to detect the activity of Factor IX in plasma and serum.PrincipleThis Factor IXa Activity Assay kit is based on the ability of FIXa to generate FXa. The generated FXa proteolytically cleaves a synthetic substrate and releases a fluorophore, AMC, which can be easily quantified by fluorescence microplate reader. The assay is simple, rapid, and can detect activity as low as 10 pg of FIXa in a variety of samples.Параметрыdetection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Factor VIIIa Activity Assay Kit (Fluorometric) MAK236-1KT
General descriptionThe coagulation Factor VIII, also known as anti-hemophilic factor (AHF) is a vital blood-clotting protein. Factor VIII circulates in the bloodstream as an inactive protein, bound to a large multimeric glycoprotein called von Willebrand factor (VWF). Upon an injury to the blood vessel, VWF dissociates from FVIII and releases the active form of FVIII (FVIIIa). In the presence of calcium ions and negatively charged membrane phospholipids, activated factor VIII (FVIIIa) then binds to the activated Factor IX (FIXa) and proteolytically activates factor X (FX) to factor Xa (FXa).
Acquired factor VIII deficiency results in skin, muscle and tissue hemorrhage.SuitabilitySuitable for the determination of Factor VIII activity in serum and plasma samplesPrincipleThis Factor VIIIa Activity Assay kit is based on the ability of FVIIIa to generate FXa. The generated FXa proteolytically cleaves a synthetic substrate and releases a fluorophore, AMC, which can be easily quantified by a fluorescence microplate reader. The assay is simple, rapid, and can detect activity as low as 1 ng of FVIIIa in a variety of samples.Параметрыdetection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Factor Xa Activity Fluorometric Assay Kit MAK238-1KT
NACRES: NA.84 General descriptionFactor Xa (FXa) is the activated form of the coagulation factor X (Stuart-Power factor, thrombokinase, prothrombinase, thromboplastin, E.C.3.4.21.6). Factor X is a vitamin K-dependent serine endopeptidase plays an important role at several stages of the coagulation pathway. It is produced in the liver and in the plasma, it circulates as a two-chain glycoprotein. It acts by converting prothrombin into active thrombin by complexing with activated co-factor V in the prothrombinase complex. Unfractionated heparin and various low molecular weight heparins bind to plasma cofactor antithrombin to inactivate several coagulation factors including factor Xa.SuitabilitySuitable for the determination of Factor Xa activity in plasma and purified Factor Xa samples.PrincipleThis Factor Xa activity assay kit utilizes the ability of Factor Xa to cleave a synthetic substrate thereby releasing a fluorophore, AMC, which can be quantified by fluorescence readers. This assay kit is simple, rapid, and can detect activity from as low as 1 ng of Factor Xa.Параметрыdetection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Factor Xa Inhibitor Screening Kit (Fluorometric) MAK239-1KT
NACRES: NA.25 General descriptionFactor Xa (FXa) is also known as Stuart-Power factor, thrombokinase, prothrombinase and thromboplastin. The proteins EC number is E.C.3.4.21.6. Factor Xa is the activated form of the coagulation factor X. Factor X is a vitamin K-dependent serine endopeptidase plays an important role at several stages of the coagulation pathway. It is produced in the liver and in the plasma, it circulates as a two-chain glycoprotein. Factor X acts by converting prothrombin into active thrombin by complexing with activated co-factor V in the prothrombinase complex. Unfractionated heparin and various low molecular weight heparins bind to plasma cofactor antithrombin to inactivate several coagulation factors including factor Xa.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for Screening potential inhibitors of Factor Xa and characterizing/studying Factor Xa inhibitors in plasma samples.PrincipleFactor Xa Inhibitor Screening Kit (Fluorometric) utilizes the ability of factor Xa to cleave a synthetic substrate, thereby releasing a fluorophore, 7-amino-4-methylcoumarin (AMC), which can be quantified by a fluorescence reader. In the presence of an inhibitor, the extent of the cleavage reaction is reduced or completely abolished. The loss in the fluorescence intensity can be correlated to the amount of inhibitor present in the assay solution. The factor Xa Inhibitor Screening Kit is a simple, straightforward, high throughput assay to screen factor Xa inhibitors.Параметрыdetection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fatty Acid Amide Hydrolase (FAAH) Activity Assay Kit (Fluorometric) MAK402-1KT
General descriptionFatty Acid Amide Hydrolase (FAAH; Oleamide Hydrolase, Anandamide Amidohydrolase) is a mammalian integral membrane enzyme, that is a key regulator in lipid signaling. FAAH degrades naturally occurring fatty acid amides such as cannabinoid anandamide and oleamide, a sleep-inducing substance. In general, fatty acid amides are endogenous lipid ligands which activate the cannabinoid (CB) G-protein coupled receptors CB1 and CB2. CB1 and CB2 modulate physiological and behavioral processes such as pain, and anti-inflammation. Therefore, FAAH is crucial in the termination of fatty acid amide bioactive functions. Recent studies have shown that blockage of FAAH activity led to increased fatty acid amides levels in nervous system and peripheral tissues. Thus, the study of FAAH and its potential inhibitors could develop novel treatment strategies for pain and central nerve system disorders due to the analgesic, anxiolytic and anti-inflammatory properties that are observed when elevated fatty acid amides are present in humans.Features and BenefitsFatty Acid Amide Hydrolase (FAAH) Activity Assay Kit:- Includes Positive Control (FAAH).
- Detects FAAH activity as low as 0.1µU.
SuitabilityThe kit is suitable for the measurement of FAAH activity of pure enzyme, in various tissues (liver and brain, etc.) and in cells (adherent or suspension cells).The kit is also suitable for the study of Fatty Acid Amide and Fatty Acid Amide Hydrolase signaling systems in various cell types.PrincipleIn this assay, FAAH hydrolyzes a non-fluorescent substrate releasing 7-amino-4-methylcoumarin (AMC), a fluorophore, which can be easily measured at ex = 360nm/em = 465nm. The kit provides a specific inhibitor that can be used to compensate for potential non-specific background in unknown samples. The stable fluorescence signal is positively correlated to FAAH enzymatic activity in samples.ПараметрыQuality Level 100, detection method fluorometric storage temp. −20°C Узнать цену -
Fatty acid extraction kit for pet food MAK372-1KT
General descriptionLipids in food serve as important energy substrates for growth and maintenance. Lipids in foods are composed primarily of triacylglycerides. Other minor components such as phospholipids, free fatty acids and cholesteryl esters may be present. The Fatty Acid Extraction Kit for Pet Food enables the extraction of these lipids in one step. The extracted lipids can then be transesterified and quantified using gas chromatography (GC) with flame-ionization detection (FID).
The Folch method has been conventionally used to extract lipids containing fatty acids from biological samples, using chloroform, methanol, and water to separate lipids from aqueous-soluble compounds. In this procedure, lipids are retained in the lower chloroform layer; whereas, aqueous-soluble compounds are retained in the upper methanol-water layer. The sample is then centrifuged to achieve uniform separation and the bottom chloroform layer is transferred with a pipette to a new test tube.
The Fatty Acid Extraction Kit for Pet Food shortens the extraction process by eliminating the need to centrifuge, pipette, and prepare solvents and standards. Once the sample is homogenized and dissolved in the Extraction Solvent containing the internal standard, it is vortexed and poured into the plunger syringe with filter, which preferentially elutes the chloroform layer containing total lipids. The user then squeezes the plunger to ensure that the lipids are eluted from the syringe filter. A portion of the total lipid extract containing the total fatty acids can then be transesterified for GC-FID analysis as described in the Procedure. Data comparing the standard Folch method to the Fatty Acid Extraction Kit for Pet Food extraction method are presented under the Results Section.
Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods. Find details here,.SuitabilityThis kit is suitable for the extraction of lipids from pet foods.Other NotesExperts have tested the Fatty Acid Extraction Kits. Watch the Video, to learn more and to see how the technology works.Параметрыusage sufficient for 40 extractions greener alternative product characteristics Waste Prevention
Learn more about the Principles of Green Chemistry,.storage temp. room temp
Safety Informationpictograms GHS02,GHS06,GHS08 signalword Danger hcodes H225 - H302 + H312 - H315 - H319 - H331 - H336 - H351 - H361d - H370 - H372 pcodes P201 - P210 - P280 - P302 + P352 + P312 - P304 + P340 + P311 - P308 + P311 Target Organs Central nervous system, Eyes Узнать цену -
Fatty Acid Extraction Kit, High Standard MAK338-1KT
General descriptionLipids are structural components of cell membranes that play a critical role in gene transcription, signaling, and metabolism. Several lipid species exist in biological systems, including phospholipids, triglycerides, and fatty acids.
When extracting fatty acids at normal or high levels, such as in food matrices, use the MAK338 fatty acid extraction kit. This kit produces stronger signals. If in doubt about which kit to use, use MAK338. When extracting fatty acids at low or very low levels, such as in cellular matrices, use the MAK174 fatty acid extraction kit. The Folch method has been conventionally used to extract lipids containing fatty acids from biological samples, using chloroform, methanol, and water to separate lipids from aqueous-soluble compounds. In this procedure, lipids are retained in the lower chloroform layer; whereas, aqueous-soluble compounds are retained in the upper methanol-water layer. The sample is then centrifuged to achieve uniform separation and the bottom chloroform layer is transferred with a pipette to a new test tube.
The Fatty Acid Extraction Kit shortens this extraction process by eliminating the need to prepare solvents and standards, centrifugation, and pipetting. Once the sample is homogenized and dissolved in the Extraction Solvent supplied in our kit, it is inverted twice and poured into the syringe containing a filter, which preferentially elutes the chloroform layer containing total lipids. A portion of the total lipid extract containing fatty acids can then be transesterified for GC-FID analysis.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods. Find details here,.SuitabilityThis kit is suitable for the extraction of lipids from a variety of samples such as whole blood, serum, biological tissue, food samples, and oil.Other NotesExperts have tested the Fatty Acid Extraction Kit. Watch the Video, to learn more and to see how the technology works.Параметрыusage sufficient for 40 extractions greener alternative product characteristics Waste Prevention
Learn more about the Principles of Green Chemistry,.storage temp. room temp
Safety Informationpictograms GHS02,GHS06,GHS08 signalword Danger hcodes H225 - H302 + H312 - H315 - H319 - H331 - H336 - H351 - H361d - H370 - H372 - H412 pcodes P201 - P210 - P273 - P280 - P302 + P352 + P312 - P308 + P311 Target Organs Central nervous system, Eyes Flash Point F 95.0 °F Flash Point C 35 °C Узнать цену -
Fatty Acid Extraction Kit, Low Standard MAK174-1KT
NACRES: NA.25 General descriptionLipids are structural components of cell membranes that play a critical role in gene transcription, signaling, and metabolism. Several lipid species exist in biological systems, including phospholipids, triglycerides, and fatty acids. When extracting fatty acids at low or very low levels, such as in cellular matrices, use the MAK174 fatty acid extraction kit. When extracting fatty acids at normal or high levels, such as in food matrices, use the MAK338 fatty acid extraction kit.
The Folch method has been conventionally used to extract lipids containing fatty acids from biological samples, using chloroform, methanol, and water to separate lipids from aqueous-soluble compounds. In this procedure, lipids are retained in the lower chloroform layer; whereas, aqueous-soluble compounds are retained in the upper methanol-water layer. The sample is then centrifuged to achieve uniform separation and the bottom chloroform layer is transferred with a pipette to a new test tube.ApplicationFatty Acid Extraction Kit has been used for fatty acid extraction.SuitabilityThis kit is suitable for the extraction of lipids from a variety of samples such as whole blood, serum, biological tissue, food samples, and oil.PrincipleThe Fatty Acid Extraction Kit shortens this extraction process by eliminating the need to prepare solvents and standards, centrifugation, and pipetting. Once the sample is homogenized and dissolved in the Extraction Solvent supplied in our kit, it is inverted twice and poured into the syringe containing a filter, which preferentially elutes the chloroform layer containing total lipids. A portion of the total lipid extract containing fatty acids can then be transesterified for GC-FID analysis.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods. Find details here,.Analysis NoteCase study can be found here: Experts have tested the Fatty Acid Extraction Kit: Time savings and greener alternative,Other NotesExperts have tested the Fatty Acid Extraction Kit. Watch the Video, to learn more and to see how the technology works.Параметрыusage sufficient for 40 extractions greener alternative product characteristics Waste Prevention
Learn more about the Principles of Green Chemistry,.storage temp. room temp
Safety Informationpictograms GHS02,GHS06,GHS08 signalword Danger hcodes H225 - H302 + H312 - H315 - H319 - H331 - H336 - H351 - H361d - H370 - H372 - H412 pcodes P201 - P210 - P273 - P280 - P302 + P352 + P312 - P308 + P311 Target Organs Central nervous system, Eyes RIDADR UN 3316 9 WGK Germany WGK 3 Flash Point F 49.5 °F - closed cup Flash Point C 9.7 °C - closed cup Узнать цену -
Fatty Acid Methyl Ester Purification Kit MAK225-1KT
General descriptionFatty Acid Methyl Ester Purification Kit is used to further purify samples prepared with the Fatty Acid Methylation Kit (MAK224). Fatty acid samples are required to be cleaned with this kit before the analysis by a capillary column.SuitabilitySuitable for the purification of methylated fatty acid samples such as blood, yeast, liver, cooking oil, soybean powder, fish oils, etc.PrincipleThe Fatty Acid Methylation Kit (Catalog Number MAK224) followed by the Fatty Acid Methyl Ester Purification Kit employs a proprietary reaction technique. Using these kits, the fatty acid methyl esterification/purification process is greatly simplified.Параметрыusage sufficient for 50 tests storage temp. room temp
Safety Informationpictograms GHS02 signalword Danger hcodes H225 pcodes P210 - P233 - P240 - P241 - P242 - P243 RIDADR UN 3316 9 Flash Point F -14.8 °F - closed cup Flash Point C -26.0 °C - closed cup Узнать цену -
Fatty Acid Methylation Kit MAK224-1KT
NACRES: NA.25 General descriptionMethyl esterification of fatty acids is commonly done prior to gas chromatography analysis to prevent peak tailing and to increase sample volatility. However, the conventional esterification procedure requires specialized equipment and high technical skill. By using the Fatty Acid Methylation kit that employs a new reaction technique, followed by the fatty acid methyl ester purification kit (MAK225), fatty acid methyl esterification is greatly simplified.SuitabilitySuitable for the methyl esterification of fatty acids (glycerolipids such as triglycerides, phospholipids, glycolipids, lecithin and sterol esters). Samples include blood, yeast, liver, cooking oil, soybean powder, fish oil, etc.PrincipleThe Fatty Acid Methylation Kit followed by the Fatty Acid Methyl Ester Purification Kit (Catalog Number MAK225) employs a proprietary reaction technique. Using these kits, the fatty acid methyl esterification/purification process is greatly simplified.Параметрыusage sufficient for 100 tests storage temp. room temp
Safety Informationpictograms GHS02,GHS05,GHS06,GHS08,GHS09 signalword Danger hcodes H225 - H251 - H290 - H301 + H311 + H331 - H304 - H314 - H336 - H361fd - H370 - H373 - H373 - H411 pcodes P210 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 Target Organs Central nervous system, Eyes, Nervous system Supp Hazards EUH014 RIDADR UN 3316 9 WGK Germany WGK 3 Flash Point F -14.8 °F Flash Point C -26 °C Узнать цену -
Fatty Acid Uptake Kit MAK156-1KT
EC Number: 200-664-3 General descriptionFatty acids are critical for many biological processes including energy metabolism and the synthesis of structural cellular components. The facilitated uptake of fatty acids, presumably through fatty acid transport proteins, is thought to be regulated at multiple levels. The dysregulation of fatty acid uptake may contribute to diseases such as obesity, type 2 diabetes, and hepatic steatosis.SuitabilityThis kit is suitable for the measurement of fatty acid uptake in cells containing fatty acid transportersPrincipleThis kit is provides a simple and sensitive method for the measurement of fatty acid uptake in cells containing fatty acid transporters. This kit uses a proprietary dodecanoic acid fluorescent fatty acid substrate whose uptake into cells result in an increase in fluorescence intensity (λex = 485/λem = 515 nm).Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Ferric Reducing Antioxidant Power (FRAP) Assay Kit (Colorimetric) MAK369-1KT
General descriptionAntioxidants are molecules which act as reducing agents by donating electrons to free radicals to stabilize them and minimize the damage caused by free radicals to DNA, cells and organ systems. Antioxidants include substances such as polyphenols; flavonoids; vitamins and enzymes like glutathione peroxidase and superoxide dismutase. They are known to have beneficial health effects such as lowering the risk of cancer, heart disease and neurodegenerative disorders and are abundantly found in plants, fruits, vegetables, beverages and natural products.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the measurement of antioxidant capacity in fruits, vegetables, beverages (like tea and wine), food products, plant extracts, herbal products, serum and plasma.PrincipleFerric reducing antioxidant power (FRAP) assay is a widely used method that uses antioxidants as reductants in a redox-linked colorimetric reaction, wherein Fe3+ is reduced to Fe2+. Ferric (Fe3+) to ferrous (Fe2+) ion reduction at low pH causes formation of a colored ferrous-probe complex from a colorless ferric-probe complex. The assay is high-throughput adaptable and can detect antioxidant capacities as low as 0.2 mM Fe2+ equivalents.Параметрыusage sufficient for 200 colorimetric tests detection method colorimetric
Safety Informationpictograms GHS05 signalword Warning hcodes H290 pcodes P234 - P390 Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
Fluorimetric Hydrogen Peroxide Assay Kit MAK166-1KT
EC Number: 231-765-0 NACRES: NA.84 General descriptionHydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.ApplicationFluorimetric Hydrogen Peroxide Assay Kit has been sued to determine the oxidation pressure (H2O2).Features and BenefitsThis assay is compatible with high-throughput handling systems.SuitabilityThe Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions.PrincipleFluorimetric Hydrogen Peroxide Assay Kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates an infrared fluorescent product (ex = 640/em = 680 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader. The generation of the fluorescence product is pH independent between pH 4 to pH 10 and is superior to other fluorescence kits for measuring hydrogen peroxide in low pH solutions which often display decreased probe sensitivity. The infrared fluorescent probe is less sensitive to assay background due to autofluorescence from biological samples. This assay is compatible with high-throughput handling systems.Параметрыusage sufficient for 500 fluorometric tests (infrared fluorescence) detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS03,GHS05,GHS07 signalword Danger hcodes H271 - H302 + H332 - H314 pcodes P220 - P280 - P305 + P351 + P338 - P310 RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fluorimetric Hydrogen Peroxide Assay Kit MAK165-1KT
General descriptionHydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.Features and BenefitsCompatible with high-throughput handling systems.SuitabilityThis kit is suitable to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions.PrincipleThe Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions. This kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates a red fluorescent product (ex = 540/em = 590 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader.Параметрыusage sufficient for 500 fluorometric tests (red fluorescence) detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H302 RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fluorometric Aldehyde Assay Kit MAK141-1KT
EC Number: 200-664-3 NACRES: NA.84 General descriptionAldehydes are highly reactive molecules, which play a role in many physiological, pathological and commercial processes. They have been implicated as environmental pollutants, and in neurodegeneration and DNA damage. A method for rapid and accurate measurement of aldehydes is an important tool for biological and chemical research, the food industry, and environmental pollution surveillance.
The Fluorometric Aldehyde Assay Kit provides a simple and direct procedure for measuring aldehydes in samples in which the pH is physiological and higher. Aldehyde reacts with a fluorogenic dye, resulting in a fluorometric (ex = 365/em = 435 nm) product proportional to the amount of aldehydes present. The Fluorometric Aldehyde Assay Kit has a lower limit of detection of 0.3 nanomole of aldehyde in a 100 µL assay volume (3 µM).
Aldehydes are highly reactive molecules, which play a role in many physiological, pathological and commercial processes. They have been implicated as environmental pollutants and in neurodegeneration and DNA damage. A method for rapid and accurate measurement of aldehydes is an important tool for biological and chemical research, the food industry and environmental pollution surveillance.SuitabilityThis kit is suitable for the detection of aldehydes in a variety of samples, particularly when the samples are at a higher pH. When working with samples in a low pH, product number MAK140, Colorimetric Aldehyde Assay, is more suitable.PrincipleThe fluorometric aldehyde assay kit provides a simple and direct procedure for measuring aldehydes in samples in which the pH is physiological and higher. Aldehyde reacts with a fluorogenic dye, resulting in a fluorometric (ex = 365/em = 435 nm) product proportional to the amount of aldehydes present. The fluorometric aldehyde assay has a lower limit of detection of 0.3nanomole of aldehyde in a 100µL assay volume (3µM).Параметрыusage sufficient for 200 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS05 signalword Warning hcodes H290 pcodes P234 - P390 RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fluorometric Intracellular pH Assay Kit MAK150-1KT
General descriptionIntracellular pH changes are implicated in diverse physiological and pathological processes, including cell proliferation, apoptosis, fertilization, malignancy, multidrug resistance, ion transport, lysosomal storage disorders, and Alzheimers disease.
This kit can be utilized to measure decreases in intracellular pH in cells treated under various conditions using a simple standard protocol. Alternatively, an Acid-Load procedure can be used to measure the increase of intracellular pH associated with changes in cellular metabolism due to GPCR activation, growth factor activity, or other cellular stimulation. With the Acid-Load procedure ammonium chloride solution is added after the fluorescent pH dye is loaded into cells in a minimum volume. This Acid-Loading step is followed by the addition of agonist in a relatively large volume (~4x) of buffer. The sudden volume change initiates an efflux of ammonia (NH3) from the cells causing a rapid decrease in intracellular pH, and thus a decrease in fluorescence signal. The effect of agonist on the subsequent recovery of intracellular pH is measured by the relative fluorescence signal increase.ApplicationFluorometric Intracellular pH Assay Kit has been used to measure the intracellular pH.Features and BenefitsSuperior alternative to BCECFSuitabilityThis kit can be utilized to measure changes in intracellular pH in cells treated under various conditions.PrincipleThe Fluorometric Intracellular pH Assay Kit utilizes a proprietary cell-permeable fluorescent indicator, BCFL-AM, for measuring relative intracellular pH changes. BCFL-AM is a superior alternative to the popular ratiometric pH probe, 2,7-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). Unlike BCECF, which is a complex and variable mixture of isomers, BCFL-AM is a single isomer species. BCFL-AM has the same spectral responses as BCECF-AM (ex = 490 nm/em = 535 nm for single measurement or ex = 505 nm/em = 535 nm and ex = 430 nm/em = 535 nm for ratio measurement) but enhanced reproducibility. BCFL-AM exhibits pH-dependent dual excitability, pKa ~7.0, and an excitation isosbestic point of 454 nm.Параметрыusage sufficient for 1000 fluorometric tests detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fluorometric Intracellular Ros Kit MAK145-1KT
NACRES: NA.25 General descriptionReactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.ApplicationFluorometric intracellular reactive oxygen species (ROS) Kit has been used for detection of intracellular ROS in live cells.SuitabilityThis kit is suitable for the detection of intracellular ROS by fluorescence microplate reader or a fluorescent microscope.PrincipleThe kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells within 1 hour incubation. ROS react with a cell-permeable sensor, resulting in a fluorometric product (ex = 520/em = 605 nm) proportional to the amount of ROS present The assay can be performed in either a 96 or 384 multiwell plate format using a fluorescence microplate reader.Параметрыusage sufficient for 200 fluorometric tests (red) detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fluorometric Intracellular Ros Kit MAK142-1KT
EC Number: 200-664-3 NACRES: NA.25 General descriptionReactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.SuitabilityThis kit is suitable for the detection of intracellular ROS by fluorescence microplate reader, flow cytometer, or a fluorescent microscope with TRITC filter.PrincipleThe Fluorometric Intracellular ROS Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells after 1 hour incubation. ROS react with a fluorogenic sensor localized to the cytoplasm, resulting in a fluorometric product proportional to the amount of ROS present. The assay can be performed in either a 96 or 384 multiwell plate format and with detection at λex = 650/λem = 675 nm.Параметрыusage sufficient for 200 fluorometric tests (Deep red) detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Fluorometric Intracellular Ros Kit MAK144-1KT
EC Number: 200-664-3 NACRES: NA.25 General descriptionReactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation above the biological system′s detoxification capacity results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.ApplicationFluorometric intracellular reactive oxygen species (ROS) Kit has been used to evaluate the mitochondrial damage by mechanical ventilation (MV). It is also used to measure the production of intracellular ROS.SuitabilityThis kit is suitable for the detection of intracellular ROS by fluorescence microplate reader, flow cytometer, or a fluorescent microscope with TRITC filter.PrincipleThe Fluorometric Intracellular ROS Assay Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour incubation. ROS react with a cell-permeable sensor, resulting in a fluorometric product (ex = 540/em = 570 nm) proportional to the amount of ROS present. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.Параметрыusage sufficient for 200 fluorometric tests (orange) detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fluorometric Intracellular Ros Kit MAK143-1KT
NACRES: NA.25 General descriptionReactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.ApplicationFluorometric Intracellular Ros Kit has been used in reactive oxygen species (ROS) generation assay.SuitabilityThis kit is suitable for the detection of intracellular ROS by fluorescence microplate reader or a fluorescent microscope.PrincipleThe Fluorometric Intracellular ROS Assay Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells within 1 hour incubation. ROS react with a fluorogenic sensor localized to the cytoplasm, resulting in a fluorometric product (ex = 490/em = 520 nm) proportional to the amount of ROS present. The assay can be performed in either a 96 or 384 multiwell plate format and read using a fluorescence microscope or a fluorescence microplate reader.
Параметрыusage sufficient for 200 fluorometric tests (green) detection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fluorometric Maleimide Assay Kit MAK167-1KT
EC Number: 204-892-4 NACRES: NA.84 General descriptionMaleimide is an unsaturated imide used in protein conjugation reactions.SuitabilityThe assay can be performed in either a 96 or 384 multiwell plate format using a fluorescence microplate reader.PrincipleThe Fluorometric Maleimide Assay Kit provides a sensitive, one-step fluorometric assay to detect maleimide, which reacts with a proprietary dye, resulting in a fluorometric product (λex = 490/λem = 520 nm) proportional to the amount of maleimide present. The assay can be performed in either a 96 or 384 multiwell plate format using a fluorescence microplate reader.Параметрыusage sufficient for 200 fluorometric tests detection method fluorometric storage temp. −20°C
Safety InformationRIDADR UN 3316 9 WGK Germany WGK 3 Узнать цену -
Fluorometric Sphingomyelin Assay Kit MAK154-1KT
NACRES: NA.84 General descriptionSphinogmyelin, a sphingolipid consisting of a phospholipid (typically phosphocholine) attached to a ceramide moiety, is a major component of cellular membranes. Sphingomylein is enriched in the exoplasmic leaflet of the cell membrane. Within the cell membrane, sphingomyelin interacts with cholesterol to form lipid rafts. The metabolism of sphingomyelin contributes to cellular signaling via the release of ceramide, a second messenger that participates in multiple cellular signaling pathways. Sphingomyelin accumulates in Niemann-Pick disease due to deficiencies in sphingomyelinase activity.SuitabilityThis kit is suitable for the detection of sphingomyelin in a variety of samples such as blood and cell extracts.PrincipleSphingomyelin is determined by a coupled enzyme assay, in which the hydrolysis of sphingomyelin by sphingomyelinase produces phosphocholine, which results in a fluorescent product (ex =540/em = 590 nm) proportional to the Sphingomyelin present.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS05,GHS08,GHS09 signalword Danger hcodes H317 - H318 - H334 - H410 pcodes P261 - P273 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 RIDADR NONH for all modes of transport Узнать цену
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