Biochemicals
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Beta-Lactamase Activity Assay Kit MAK221-1KT
NACRES: NA.84 General description-Lactamase (bL; EC 3.5.2.6) is an enzyme first identified in Escherichia coli and has been described as penicillinase. A number of Ls have since been identified from various bacteria. Ls specifically hydrolyze -lactam rings present in antibiotics such as penicillin, cephalosporins, monobactam, and carbapenem, and confer resistance against these antibiotics.SuitabilitySuitable for the detection of β-lactamase activity in biological samples, food, and fermentation samples.PrincipleThe β-Lactamase Activity Assay Kit provides a simple assay for measuring βL activity in βL-secreting bacteria in fermentation media and bacterial cultures. It can also be used to detect βL-secreting bacteria in saliva, urine, and serum of infected mammals and in food samples. βL activity is measured by hydrolyzing a chromogenic cephalosporin called nitrocefin, producing a colorimetric product (A490), proportional to the enzymatic activity present. One unit of β-lactamase is the amount of enzyme required to hydrolyze 1.0 μmole of nitrocefin per minute at pH 7.0 at 25 °C.Параметрыdetection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 pcodes P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 RIDADR NONH for all modes of transport Узнать цену -
Beta-Lactamase Inhibitor Screening Kit MAK222-1KT
General description-Lactamase (-lactamase, L, EC 3.5.2.6) is an enzyme first identified in Escherichia coli and has been described as penicillinase. A number of Ls have since been identified from various bacteria. -Lactamases specifically hydrolyze -lactam rings present in antibiotics such as penicillin, cephalosporins, monobactam, and carbapenem, and confer resistance against these antibiotics.ApplicationBeta-Lactamase Inhibitor Screening Kit has been used in enzymatic assays.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the screening of inhibitors of β-LactamasePrincipleThe β-Lactamase Inhibitor Screening Kit is a rapid, simple and sensitive assay that is suitable for high throughput screening of β-Lactamase inhibitors. βL activity is measured by hydrolyzing a chromogenic cephalosporin called nitrocefin, producing a colorimetric product (A490), proportional to the enzymatic activity present.Параметрыdetection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 pcodes P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 RIDADR NONH for all modes of transport Узнать цену -
Beta-Secretase Activity Fluorometric Assay Kit MAK237-1KT
NACRES: NA.84 General descriptionβ-Secretase has been implicated to be an excellent target for anti-amyloid therapy for the treatment of Alzheimers disease.SuitabilitySuitable for the determination of -Secretase activity in biological and purified samples.PrincipleThe β-Secretase activity Assay Kit provides a convenient fluorescence method for detecting β-secretase activity in biological and purified samples. The assay utilizes a secretase-specific peptide conjugated to two reporter molecules EDANS and DABCYL. In the uncleaved form, the fluorescent emissions from EDANS are quenched by the physical proximity of the DABCYL moiety. Cleavage of the peptide by secretase physically separates EDANS and DABCYL allowing for the release of a fluorescent signal. The level of secretase enzymatic activity in samples is proportional to the level of fluorescence intensity.Параметрыdetection method fluorometric storage temp. −70°C
Safety Informationpictograms GHS05 signalword Danger hcodes H318 pcodes P280 - P305 + P351 + P338 + P310 RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Bile Acid Assay Kit MAK309-1KT
NACRES: NA.84 General descriptionTwelve different types of bile acids are typically found in mammals, among them two primary types are cholic acid and chenodeoxycholic acid. These can be dehydroxylated into secondary bile acids. Finally, these four can be conjugated to either taurine or glycine creating 8 different conjugated bile acids. Bile acid levels in feces, blood, urine, and bile can be used as markers for various diseases such as hyperlipidemia, cholestasis, gall stones, colon cancer, etc. Bile acids also exist as sulfate salt forms known as bile acid sulfates. Sulfation of bile acids increases their solubility and decreases intestinal absorption, thereby enhancing fecal and urinary excretion.ApplicationBile Acid Assay Kit has been used to quantify bile acid levels from plasma.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism in serum, plasma, urine and other biological samples.PrincipleThis Bile Acid Assay Kit provides a convenient fluorimetric means to measure total bile acids in biological samples. In the assay, 3–hydroxysteroid dehydrogenase reacts with all twelve bile acids, converting NAD to NADH, which reduces a probe to a highly fluorescent product. The resulting fluorescence intensity (γex = 530 nm/γem = 585 nm) is linear to the bile acid concentration in the sample.Параметрыdetection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Bilirubin Assay Kit MAK126-1KT
NACRES: NA.84 General descriptionBilirubin, also known as hematoidin, is a degradation product formed as a result of heme catabolism in the liver. Bilirubin circulates in the blood stream as either the unconjugated insoluble form (indirect bilirubin) or the soluble glucuronide-conjugated form (direct bilirubin). Conjugated bilirubin moves from the bile canaliculi of the liver to the gall bladder where it is excreted into the small intestine during digestion. High levels of bilirubin can result in jaundice and may indicate liver disease, blood disorders, or blockage of the bile ducts.ApplicationBilirubin Assay Kit has been used to measure the total bilirubin concentration in samples.Features and BenefitsCompatible with high-throughput handling systems. Can be adapted for use with cuvettes.SuitabilitySuitable for the detection of of both total and conjugated bilirubin in serum and plasma samples.PrincipleThis assay, based on the Jendrassik-Grof method, utilizes the reaction of bilirubin with diazotized sulfanilic acid resulting in a colorimetric product measured at 530 nm, proportionate to the bilirubin present in the sample. This assay kit measures both total and conjugated bilirubin.Параметрыusage sufficient for 180 colorimetric tests detection method colorimetric storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Branched Chain Amino Acid Kit MAK003-1KT
General descriptionThe branched-chain amino acids (BCAA), leucine, isoleucine, and valine, are the most common essential amino acids in proteins. The BCAA may function in both energy production and as nutrient signals. Dietary supplementation with BCAA may aid in the treatment of various disorders such as Amyotrophic Lateral Sclerosis (ALS), latent and chronic hepatic encephalopathies, and to prevent muscle atrophy in cancer patients. BCAA may increase the rate of protein synthesis and sensitize cells to insulin and insulin-like growth factor.ApplicationBranched Chain Amino Acid Kit has been used to determine the concentration of hepatic branched-chain amino acids, in a study assessing the role of CREB regulated transcription coactivator 2 (CRTC2) in hepatic lipid homeostasis and obesity in mice.SuitabilitySuitable for measuring Branched Chain Amino Acids (BCAAs) in a variety of biological samples.PrincipleIn this assay, BCAA concentration is determined using a coupled enzyme reaction, which results in a colorimetric (450 nm) product, proportional to the BCAA present. The BCAA Assay Kit provides a simple convenient means of measuring the BCAAs in a variety of biological samples.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Butyrylcholinesterase Activity Kit (Colorimetric) MAK406-1KT
General descriptionButyrylcholinesterase (BChE), also known as plasma cholinesterase or pseudocholinesterase, is a serine hydrolase present in almost all mammalian tissues with the highest levels detected in plasma and liver. BChE hydrolyzes choline esters, as well as other esters and acts as an endogenous scavenger for anticholinesterase agents. BChE in plasma serves as the first line of defense against toxic compounds reaching the bloodstream that might inhibit acetylcholinesterase activity, which is essential in the nervous system. In clinical toxicology and clinical chemistry, determination of BChE activity in plasma is the most commonly used and preferred method to diagnose patients showing symptoms of intoxication. A recent study using genome-wide analysis suggested that BChE is a marker of obesity, insulin resistance and metabolic syndrome, hyperlipidemia, coronary artery disease and hypertension. Serum BChE has been proposed as a marker of low-grade systemic inflammation and a marker of cardiovascular risk factor being even capable to predict mortality.Features and BenefitsButyrylcholinesterase Activity Kit:- Includes Positive Control. (Butyrylcholinesterase)
- Detects as low as 0.2 U/mL in a variety of samples.
SuitabilityThe kit is suitable for the measurement of BChE activity and the screening of BChE inhibitors in biological samples. Biological samples include serum, plasma, blood and tissue homogenates (liver, lung, etc).PrincipleThe Butyrylcholinesterase Activity Kit is based on the ability of BChE to hydrolyze substrate and produce thiocholine. Thiocholine reacts with 5, 5-dithiobis (2-nitrobenzoic acid) (DTNB) and generates a yellow chromophore that can be quantified at A412 nm.ПараметрыQuality Level 100, detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05 signalword Danger hcodes H315 - H318 pcodes P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P362 + P364 Узнать цену -
c-Src Kinase Inhibitor Screening Kit MAK426-1KT
General descriptionCellular Src Kinase (c-Src) is a non-receptor tyrosine kinase that regulates a wide array of cellular signal transduction pathways by phosphorylation of specific tyrosine residues in other tyrosine kinases. Src kinase family members interact with many proteins such as receptor tyrosine kinases, GPCRs, ion channels, steroid receptors, transcription activators, solute transporters and transmembrane adhesion receptors. c-Src was the first “proto-oncogene” to be identified. Over-expression of wild type c-Src or expression of a constitutively active mutant form is frequently found in a number of different cancers. Activation of c-Src enhances angiogenesis, proliferation and invasion pathways in tumors. The extent of this activation typically correlates with the malignant potential and patient survival. Despite the significant role of c-Src in oncogenesis, there are no known selective c-Src inhibitors. A number of clinically used tyrosine kinase inhibitors are capable of inhibiting c-Src. However, the currently available drugs are not selective and inhibit multiple kinases. Selective c-Src inhibitors may be more efficacious and can exhibit fewer side effects than the promiscuous multi-kinase inhibitors.Features and Benefitsc-Src Kinase Inhibitor Screening Kit:- Compatible with high-throughput handling systems
- Highly sensitive
SuitabilityThe kit is suitable for screening and characterization of drugs and novel chemical entities for inhibition or modulation of c-Src activity and for the development of structure-activity relationship models to predict Src kinase selectivity of compounds.PrincipleThe c-Src Kinase Inhibitor Screening Kit enables rapid screening of test compounds for modulation of c-Src activity. The assay uses a c-Src-specific polypeptide substrate and a high concentration of the ultra-pure ATP that closely reflects the physiological ATP levels. ADP formation during the kinase reaction is measured. The strong and stable fluorescence signal (ex = 535 nm/em = 587 nm) generated during the reaction is directly proportional to the amount of ADP generated. This ensures a high signal-to-background ratio and little interference due to short wavelength fluorescence by test compounds.ПараметрыQuality Level 100, detection method fluorometric storage temp. −70°C Узнать цену -
Calcium Colorimetric Assay MAK022-1KT
NACRES: NA.84 General descriptionCalcium, the most abundant mineral in the human body, is a crucial intracellular element that is responsible for regulating many physiological and pathological processes. Calcium is found in either the free ion form or in bound complexes, for example the calcium phosphate and calcium carbonate complexes that make up bone tissue. Numerous physiological processes, including muscle contraction, cell adhesion, hormones/ neurotransmitters release, glycogen metabolism, cell proliferation/differentiation, blood clotting, nerve or synapthetic impulse transmission, and structural support of the skeleton are regulated by calcium signaling. Defects in the integrity of cell-specific calcium signaling systems may be associated with certain human diseases.ApplicationCalcium Colorimetric Assay kit has been used to measure calcium concentration in hippocampal samples and MC3T3-E1 mouse osteoblast cell line, which were cultured in osteogenic induction medium.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for use with cell and tissue culture supernatants, urine, plasma, serum, fecal material, media, and other biological fluids.PrincipleIn this assay, the calcium ion concentration is determined by the chromogenic complex formed between calcium ions and o-cresolphthalein, which is measured at 575 nm and is proportional to the concentration of calcium ions present. The linear range of detection for this kit is between 0.4-2.0 µg.Параметрыusage sufficient for 250 colorimetric tests detection method colorimetric storage temp. 2-8°C
Safety InformationPictograms GHS05,GHS08,GHS09 Signal Word Danger Hazard Statements H290 - H319 - H360D - H411 Precautionary Statements P201 - P273 - P305 + P351 + P338 - P308 + P313 RIDADR NONH for all modes of transport Узнать цену -
Calpain Activity Fluorometric Assay Kit MAK228-1KT
General descriptionActivation of calpain is involved in many forms of physiological and pathological processes (e.g., apoptosis). Calpain activation requires cell membrane and Ca2+, and activated calpain is released into cytosol. The Calpain Activity Assay Kit provides optimized buffers and reagents for a convenient measurement of calpain activity.SuitabilitySuitable for analyzing calpain activity in apoptotic and other samples.PrincipleCalpain Activity Fluorometric Assay Kit is based on the cleavage of the calpain substrate, Ac-LLY-AFC. The Ac-LLY-AFC substrate emits blue light (max = 400 nm); upon cleavage by calpain, free AFC emits a yellow-green fluorescence ( max = 505 nm), which can be quantified using a fluorometer or a fluorescence plate reader. Comparison of the fluorescence intensity from a treated sample with a normal control allows determination of the changes in calpain activity.Параметрыdetection method fluorometric storage temp. −70°C
Safety Informationpictograms GHS05,GHS06,GHS09 signalword Danger hcodes H302 + H312 - H317 - H318 - H331 - H411 pcodes P261 - P280 - P280 - P304 + P340 + P311 - P305 + P351 + P338 + P310 - P403 + P233 RIDADR UN 3316 9 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) MAK404-1KT
General descriptionCarbonic anhydrases (CA) are zinc enzymes present in both prokaryotes and eukaryotes. They catalyze efficiently the reversible hydration of CO2 to bicarbonate. Their important patho-physiological roles in respiration, pH and CO2 homeostasis, secretion, gluconeogenesis, ureagenesis etc. make it an important drug target. Characteristic increase and decrease of CA Activity is observed in different physiological conditions and diseases such as anemia, Thalassemia, Hypothyroidism, Hyperthyroidism, and in several cases of lung and liver diseases and Leukemia.Features and BenefitsCarbonic Anhydrase (CA) Activity Assay Kit:- Compatible with high-throughput handling systems.
SuitabilityThe kit is suitable for the detection of carbonic anhydrase activity in hemolysates and serum, as well as the determination of enzymatic activity of purified CA.PrincipleThe assay utilizes the esterase activity of an active CA on an ester substrate which releases a chromophore.The released product can be easily quantified using a spectrophotometric multi-well plate reader. In the presence of a CA specific inhibitor, the enzyme loses its activity which results in a decrease of absorbance.To measure specific CA activity, the CA inhibitor Acetazolamide is included.Acetazolamide is a potent inhibitor of CA I, CA II, CA IV, CA IX, CA XII etc. except CA III.ПараметрыQuality Level 100, detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05,GHS08 signalword Danger hcodes H315 - H318 - H334 pcodes P261 - P264 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 Узнать цену -
Cardiolipin Assay Kit MAK362-1KT
General descriptionCardiolipin (CL) is a very important phospholipid present in the inner mitochondrial membrane and constitutes about 20% of its total lipid composition. It has a dimeric structure comprised of two phosphatidic acid residues linked by a glycerol bridge. It is essential for several mitochondrial functions such as maintaining activity of the electron transport chain complexes, and other mitochondrial processes including biogenesis, fission, fusion and protein transport. It is involved in apoptosis where it helps alter the mitochondrial membrane structure and aids in the release of cytochrome c. Conditions such as diabetes and heart failure are linked to changes in levels of cardiolipin and it is also depleted during aging. Exposure to toxicants like cigarette smoke and organophosphates may cause an alteration in cardiolipin levels and composition, thus adversely affecting health.SuitabilitySuitable for the measurement of cardiolipin content in cell lysates and isolated mitochondria.PrincipleThe Cardiolipin Assay Kit is a fluorometric assay that makes use of a proprietary probe that fluoresces on association with cardiolipin but not with any other lipids such as phosphatidylcholine and sphingomyelin, making it highly specific. Fluorescence can be recorded at ex = 340 nm/em = 480 nm. The kit includes purified cardiolipin as standard and can detect as low as 0.2 nmol of cardiolipin.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS02,GHS07 signalword Danger hcodes H225 - H319 pcodes P210 - P305 + P351 + P338 Flash Point F 49.5 °F Flash Point C 9.7 °C Узнать цену -
Carrez Clarification Reagent Kit MAK191-1KT
NACRES: NA.25 General descriptionThe Carrez Clarification Reagent Kit is suitable for the preparation of samples for the analysis of small molecules such as carbohydrates, alcohols, aldehydes, and organic acids. The samples might contain proteins, fats, and redox compounds that interfere with the analysis.ApplicationThe Carrez Clarification Reagent Kit can be used for the preparation of food (such as meat, cereal, or milk) or blood for further analysis (such as HPLC or bioassays). The reagents in this kit cannot be used to prepare samples that contain or may be converted to ascorbate, ammonia, or citrate in enzyme-based assays.SuitabilitySuitable for the clarification of food, beverage, and other samples for use in downstream analyses.PrincipleThe Carrez Clarification Reagents remove interfering compounds from the analytes and are more simple, less expensive, and less time-consuming than using spin filters.Параметрыusage sufficient for 100 tests storage temp. room temp
Safety Informationpictograms GHS05,GHS09 signalword Danger hcodes H318 - H410 pcodes P273 - P280 - P305 + P351 + P338 - P391 - P501 RIDADR NONH for all modes of transport WGK Germany WGK 3 Узнать цену -
Catalase Assay Kit MAK381-1KT
General descriptionCatalase is a ubiquitous antioxidant enzyme that is present in nearly all living organisms. It functions to catalyze the decomposition of hydrogen peroxide (H202) to water and oxygen.Features and BenefitsThe Catalase Assay Kit is:- Compatible with high-throughput handling systems.
- Simple and rapid procedure with results in 40 minutes.
SuitabilityThe kit is suitable for the determination of catalase activity in a variety of biological samples.PrincipleIn the assay, catalase first reacts with H202 to produce water and oxygen, the unconverted H202 reacts with the probe to produce a product, which can be measured colorimetrically at A570 nm or fluorometrically at ex = 535 nm/em = 587 nm. Catalase activity is reversely proportional to the signal. The kit can detect 1 mU or less of catalase activity in samples.ПараметрыQuality Level 100, detection method colorimetric, fluorometric storage temp. 2-8°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 - H412 pcodes P261 - P272 - P273 - P280 - P284 - P302 + P352 Узнать цену -
Catalase Assay Kit
NACRES: NA.84
Кат. номер CAT100-1KT CAT100-1KT-PW General descriptionThe Catalase Assay Kit contains all necessary components for studying catalase activity in various tissues and subcellular organelles.ApplicationSutitable for Colorimetric and UV AssaysBiochem/physiol ActionsCatalase is a ubiquitous antioxidant enzyme which catalyses the decomposition of hydrogen peroxide (H2O2) to water and oxygen. Hydrogen peroxide is formed in the eukaryotic cell as a by-product of various oxidases and superoxide dismutases. Hydrogen peroxide accumulation in cells causes oxidation of cellular targets such as DNA, proteins, and lipids leading to mutagenesis and cell death. Removal of the H2O2 from the cell by catalase provides protection against oxidative damage to living cells and its role in oxidative stress related diseases has been widely studied.Features and Benefits- Useful for determining catalase activity - may be used in various tissues and cells
- Simple, optimized protocol - A simple colorimetric assay for analysis of peroxisome enrichment and catalase activity
SuitabilitySuitable for studying catalase activity in various tissues and subcellular organelles.PrincipleThis assay method is based on the measurement of the hydrogen peroxide substrate remaining after the action of catalase. First, the catalase converts hydrogen peroxide to water and oxygen (catalatic pathway) and then this enzymatic reaction is stopped with sodium azide. An aliquot of the reaction mix is then assayed for the amount of hydrogen peroxide remaining by a colorimetric method.10 The colorimetric method uses a substituted phenol (3,5-dichloro-2-hydroxybenzenesulfonic acid), which couples oxidatively to 4-aminoantipyrine in the presence of hydrogen peroxide and horseradish peroxidase (HRP) to give a red quinoneimine dye (N-(4-antipyryl)-3-chloro-5-sulfonatep-benzoquinone-monoimine) that absorbs at 520 nmUnit DefinitionOne unit of catalase will decompose 1.0 micromole of hydrogen peroxide to oxygen and water per minute at pH 7.0 at 25 °C at a substrate concentration of 50 mM hydrogen peroxide.Preparation NoteUse ultrapure water in preparation of all solutions.ПараметрыQuality Level 200, usage sufficient for ≥100 tests enzymatic detection method colorimetric shipped in wet ice storage temp. 2-8°C Gene Information human ... CAT(847)
Safety Informationpictograms GHS08 signalword Danger hcodes H334 pcodes P261 - P284 - P501 Personal Protective Equipment Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Cathepsin B Activity Assay Kit MAK387-1KT
General descriptionApoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.Features and BenefitsThe Cathepsin-B Activity Assay kit:- Is a simple one-step procedure with results in 1-2 hours.
SuitabilityThe kit is suitable for the detection of Cathepsin-B activity in cell lysates.PrincipleThe Cathepsin-B Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-B substrate sequence RR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-B will cleave the synthetic substrate RR-AFC to release free AFC. The released AFC can easily be quantified using a fluorescence plate reader.ПараметрыQuality Level 100, detection method fluorometric storage temp. −20°C Узнать цену -
Cathepsin B Inhibitor Screening Kit MAK200-1KT
NACRES: NA.25 General descriptionCathepsin B (CTSB; EC 3.4.22.1) is a lysosomal cysteine proteinase involved in intracellular degradation and turnover of proteins. It is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP is a causative factor in Alzheimers disease. CTSB has also been implicated in the invasion and metastasis of certain cancers.
The Cathepsin B Inhibitor Screening Kit is a simple assay for screening potential inhibitors of CTSB. CTSB activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-based substrate to yield AFC, a fluorescent product (ex = 400/em = 505 nm), proportional to the enzymatic activity present.SuitabilitySuitable for the screening of inhibitors of Cathepsin B.PrincipleThe Cathepsin B Inhibitor Screening Kit is a simple assay for screening potential inhibitors of CTSB. CTSB activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-based substrate to yield AFC, a fluorescent product (ex = 400/em = 505 nm), proportional to the enzymatic activity present.Параметрыdetection method fluorometric storage temp. 20°C Gene Information human ... CTSB(1508)
mouse ... CTSB(13030)
rat ... CTSB(64529)
Safety Informationpictograms GHS07 signalword Warning hcodes H319 pcodes P264 - P280 - P305 + P351 + P338 - P337 + P313 RIDADR NONH for all modes of transport Узнать цену -
Cathepsin D Assay Kit MAK383-1KT
General descriptionApoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.Features and BenefitsThe Cathepsin-D Activity Assay kit:- Is a simple one-step procedure with results in 1-2 hours.
SuitabilityThe kit is suitable for the detection of Cathepsin-D activity in cell lysates.PrincipleThe Cathepsin-D Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-D substrate sequence GKPILFFRLK(Dnp)-DR-NH2) labeled with 7-Methoxycoumarin-4-acetic acid (MCA). Cell lysates or other samples that contain cathepsin-D will cleave the synthetic substrate to release fluorescence, which can then easily be quantified fluorometrically at ex = 328 nm/em = 460nm.ПараметрыQuality Level 100, detection method fluorometric storage temp. −20°C Узнать цену -
Cathepsin K Inhibitor Screening Kit MAK201-1KT
NACRES: NA.25 General descriptionCathepsin K (CTSK; EC 3.4.22.38) is a lysosomal cysteine proteinase expressed predominantly in the osteoclasts. It exhibits potent collagenase activity and is involved in bone remodeling and resorption. Mutations in CTSK gene result in pycnodysostosis characterized by osteosclerosis and short stature.SuitabilitySuitable for the screening of inhibitors of Cathepsin K.PrincipleThe Cathepsin K Inhibitor Screening Kit is a simple assay for screening potential inhibitors of CTSK. CTSK activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-based substrate to yield AFC, a fluorescent product (ex = 400/ em = 505 nm), proportional to the enzymatic activity present.Параметрыdetection method fluorometric shipped in dry ice storage temp. 70°C Gene Information human ... CTSK(1513)
mouse ... CTSK(13038)
rat ... CTSK(29175)
Safety Informationpictograms GHS07 signalword Warning hcodes H317 - H319 pcodes P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338 RIDADR NONH for all modes of transport Узнать цену -
Cathepsin L Activity Assay Kit MAK401-1KT
General descriptionApoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.Features and BenefitsCathepsin L Activity Assay Kit:- Simple procedure with results in 1-2 hours.
SuitabilityThe kit is suitable for the measurement of cathepsin L in cell lysates.PrincipleThe Cathepsin L Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin L substrate sequence FR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin L will cleave the synthetic substrate FR-AFC to release free AFC. The released AFC can easily be quantified fluorometrically. The cathepsin L assay is simple and straightforward. Assay conditions have been optimized to obtain the maximal activity.ПараметрыQuality Level 100, detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS05,GHS08 signalword Danger hcodes H315 - H318 - H334 pcodes P261 - P264 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 Узнать цену -
Cathepsin S Assay Kit MAK403-1KT
General descriptionApoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.Features and BenefitsCathepsin S Activity Assay Kit:- Simple procedure with results in 1-2 hours.
SuitabilityThe kit is suitable for the measurement of cathepsin S in cell lysates.PrincipleThe Cathepsin S Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin S substrate sequence VVR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin S will cleave the synthetic substrate Z-VVR-AFC to release free AFC. The released AFC can easily be quantified fluorometrically. The cathepsin S assay is simple and straightforward. Assay conditions have been optimized to obtain the maximal activity.ПараметрыQuality Level 100, detection method fluorometric storage temp. −20°C Узнать цену -
Cathepsin S Inhibitor Screening Kit MAK202-1KT
General descriptionCathepsin S (CTSS; EC 3.4.22.27) is a lysosomal cysteine proteinase involved in the degradation of proteins to peptides required for the presentation of MHC class II molecules.1 It is associated with pathological conditions such as atherosclerosis, coronary heart disease, cancer, and insulin resistance.SuitabilitySuitable for the screening of inhibitors of Cathepsin S.PrincipleThe Cathepsin S Inhibitor Screening Kit is a simple assay for screening potential inhibitors of CTSS. CTSS activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-based substrate to yield AFC, a fluorescent product (ex = 400/ em = 505 nm), proportional to the enzymatic activity present.Параметрыdetection method fluorometric storage temp. 20°C Gene Information human ... CTSS(1520)
mouse ... CTSS(13040)
rat ... CTSS(50654)
Safety Informationpictograms GHS07 signalword Warning hcodes H317 pcodes P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 RIDADR NONH for all modes of transport Узнать цену -
Cell Cycle Analysis Kit MAK344-1KT
General descriptionCell cycle is a ubiquitous, complex sequence of events leading to growth and proliferation of cells. Cell cycle progression is tightly regulated due to its involvement in development, DNA damage and repair, etc. Anomalies in cell cycle progression can lead to tissue hyperplasia and diseases such as cancer. Cell cycle can be subdivided into interphase (G0/G1, S and G2) and mitotic (M) phase (prophase, metaphase, anaphase and telophase).Features and BenefitsThe method is non-radioactive, rapid and accurate and can be used for high throughput cell cycle analysis with contemporary flow cytometer instruments.SuitabilitySuitable for studying cell cycle in adherent or suspension cellsPrincipleThe kit utilizes a nuclear dye, the binding of which to nucleic acids in the cell results in fluorescence signal, which is proportional to cellular DNA content. The percentages of cells in different phases of the cell cycle (G0/G1, S, and G2/M) can be quantified by flow cytometry.Параметрыusage sufficient for 100 assays detection method fluorometric storage temp. −20°C
Safety InformationFlash Point F Not applicable Flash Point C Not applicable Узнать цену -
Ceruloplasmin Activity Colorimetric Kit MAK177-1KT
NACRES: NA.25 General descriptionCeruloplasmin is a multifunctional copper-containing ferroxidase present in serum. Ceruolpasmin is the major transporter of copper in the blood, carrying more than 95% of the copper found in plasma. It uses copper to couple the oxidation of substrate with the reduction of oxygen to water. Ceruloplasmin-mediated oxidation of ferrous (Fe2+) iron to ferric (Fe3+) iron contributes to the regulation of iron efflux in cells that have transportable iron stores. Inherited loss-of-function mutations in ceruloplasmin has been linked to aceruloplasminemia. Ceruloplasmin is known to form complexes with cationic proteins during inflammation and is also involved in preventing iron-induced oxidative damage. Increased ceruloplasmin levels have been observed in celiac and Crohns patients. Elevated serum ceruloplasmin has also been linked to cancer, pregnancy, rheumatoid arthritis, schizophrenia, and Alzheimers disease, whereas its low levels have been linked to Wilsons and Menkes diseases. Hence, measurement of ceruloplasmin activity in biological samples can provide useful insights into several metabolic and pathological conditions.ApplicationCeruloplasmin Activity Colorimetric Kit has been used to measure serum ceruloplasmin quantity and activity.SuitabilitySuitable for the detection of ceruloplasmin activity in serum.PrincipleThe Ceruloplasmin Activity Colorimetric Kit provides a fast and easy-to-use procedure for measuring ceruloplasmin activity in serum. Ceruloplasmin activity is determined using substrate oxidation, which results in a colorimetric (560 nm) product proportional to the enzymatic activity present. One unit of ceruloplasmin is the amount of enzyme that will oxidize 1 μmole of substrate per minute at 25 °C.Параметрыdetection method colorimetric storage temp. 2-8°C Gene Information human ... CP(1356)
mouse ... CP(12870)
rat ... CP(24268)
Safety Informationhcodes H412 pcodes P273 - P501 RIDADR NONH for all modes of transport Узнать цену -
CETP Activity Assay Kit MAK106-1KT
NACRES: NA.84 General descriptionCholesteryl ester transfer protein (CETP) is present in normal human plasma. The protein transfers neutral lipids from high density lipoproteins (HDL) to very low density lipoprotein (VLDL) and low density lipoprotein (LDL). CETP plays an important role in lipoprotein metabolism and influences the reverse cholesterol transport pathway. The method is useful for measuring CETP activity in plasma or serum in all species that express CETP.ApplicationCholesteryl ester transfer protein (CETP) activity Assay Kit has been used for measurement of CETP activity in the plasma samples.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for the detection of cholesteryl ester transfer protein (CETP) in plasma / serum (fresh or frozen), recombinant or purified CETP.PrincipleThe CETP Activity Assay Kit uses a proprietary substrate that enables the detection of CETP-mediated transfer of neutral lipid from the substrate to a physiological acceptor. The transfer activity results in an increase in fluorescence intensity (λex=535/λem=587). In a total volume of 200 μL, the assay is linear from 0.2 to 0.8 μL of normal human plasma. Assay results not affected by endogenous plasma HDL, LDL or VLDL concentrations.Analysis NoteLearn more about Roar Assay Kits for Studies in Lipoprotein Metabolism.,Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. 2-8°C Gene Information human ... CETP(1071)
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
CETP RP Activity Assay MAK305-1KT
NACRES: NA.84 General descriptionCholesteryl ester transfer protein (CETP) is present in normal human plasma and transfers neutral lipids from high density lipoproteins (HDL) to very low density lipoprotein (VLDL) and low density lipoprotein (LDL). CETP plays an important role in lipoprotein metabolism and influences the reverse cholesterol transport pathway.Features and BenefitsSynthetic assay components support manipulation for MOA studies and SAR work. Unusually high signal-to-background with linearity. Assay components stable for up to 1 year. High DMSO tolerance.SuitabilitySuitable for high-throughput screening (HTS), mechanism of action (MOA) studies, and structure-activity relationship (SAR) work in CETP sources.PrincipleThe CETP RP Activity Assay uses a proprietary substrate that enables the detection of CETP-mediated neutral lipid mass transfer. The method is useful for measuring CETP activity in recombinant protein (RP) or purified CETP samples and has a high DMSO tolerance. The transfer activity results in an increase in fluorescence intensity (λex = 465/λem = 535 nm). With recombinant CETP as the CETP source, the assay is linear over a wide range of CETP concentrations and provides very high signal-to-background values. Typical Z factor: ∼0.9Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Chloride Assay Kit MAK023-1KT
NACRES: NA.84 General descriptionChloride is an essential anion needed for the proper functioning of many critical aspects of metabolism, including aiding in maintenance of the bodys acid-base balance. In Cystic Fibrosis, mutations to the CF transmembrane conductance regulator (CFTCR) gene result in altered sodium and chloride ion transport channels.ApplicationChloride Assay Kit has been used to measure chloride concentration spectrophotometrically and is used to measure chloride levels in blood plasma of toads Rhinella marina.SuitabilitySuitable for the quantification of Chloride in a variety of biological samples. Blood and urine can be used directly after dilution with waterPrincipleThe Chloride Assay kit provides a simple and direct procedure for measuring chloride in a variety of samples, including blood and urine. Chloride concentration is determined by a competition reaction between Hg2+ and Fe2+ for 2,4,6-Tris(2-pyridyl)-s-triazine(TPTZ). The preferred Hg-TPTZ complex exhibits no color. In the presence of chloride, Hg2+ forms HgCl2, which precipitates, allowing TPTZ to complex with Fe2+. The Fe-TPTZ complex results in a colorimetric (620 nm) product proportional to the chloride present.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. room temp
Safety Informationpictograms GHS08 signalword Warning hcodes H373 - H412 pcodes P260 - P273 - P314 - P501 RIDADR NONH for all modes of transport Узнать цену -
Cholesterol Efflux Assay Kit MAK192-1KT
General descriptionReverse Cholesterol Transport (RCT) is the mechanism by which the peripheral lipid burden is reduced by transport to liver and its fecal excretion. The first important step in RCT is the cholesterol efflux from macrophage-derived foam cells present in atherosclerotic plaques. The cholesterol efflux is critical for the maintenance of net cholesterol balance in arterial walls and in the reduction of proinflammatory responses triggered by lipid-laden macrophages.ApplicationCholesterol Efflux Assay Kit has been used in cholesterol efflux assay.SuitabilitySuitable for the determination of cholesterol efflux in cells.PrincipleThe Cholesterol Efflux Assay Kit is a high-throughput screening assay that measures cholesterol efflux in cells using fluorescent-labeled cholesterol (ex = 482/ em = 515 nm). It is suitable for serum, isolated, or recombinant lipoprotein samples. It may also be used to screen small molecules for their effect on cholesterol efflux and therefore, may be a valuable tool in drug discovery programs.Параметрыdetection method fluorometric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Cholesterol Extraction Kit MAK175-1KT
NACRES: NA.25 General descriptionCholesterol is a structural component of cell membranes and a precursor to hormones. The Cholesterol Extraction Kit enables the extraction of sterols including cholesterol from biological samples in two simple steps. The extracted sterols can then be saponified, derivatized, and quantified using gas chromatography (GC) with flame-ionization detection (FID) or gas chromatography/mass spectrometry (GC/MS).SuitabilityThis kit is suitable for the extraction of cholesterol and cholesterol-like compounds (sterols) from whole blood, serum, biological tissue and food samples.PrincipleSterols including cholesterol are typically extracted using a dual solvent partition system containing a lipophilic solvent and an aqueous solvent. Sterols are retained in the lower chloroform phase, whereas, aqueous-soluble compounds are retained in the methanol-water layer. The sample is then centrifuged and the bottom chloroform layer is transferred with a pipette to another test tube. An aliquot of the transferred layer is then saponified with methanolic NaOH or KOH, and then derivatized with trimethylsilyl chloride (chlorotrimethylsilane). The formed cholesterol-TMS ester is reconstituted with hexane and injected directly into a GC-FID or GC/MS system for quantification.
The Cholesterol Extraction Kit shortens the extraction process by eliminating the need to prepare solvents and standards, centrifugation, and pipetting. Once the sample is homogenized and dissolved in the Extraction Solvent containing the internal standard, it is inverted twice and poured into the syringe containing a filter, which preferentially elutes the chloroform layer containing sterols. A portion of the total sterol extract can be saponified and derivatized for GC-FID or GC/MS analysis as described in the procedure.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods.Find details here,.Analysis NoteClick on the following link for a demo: Easy to Use High-Throughput Kits for Cholesterol and Lipid Extractions,Other NotesEasy to Use High-Throughput Kits for Cholesterol and Lipid Extractions. Watch the Video, to learn more and to see how the technology works.Параметрыusage sufficient for 40 extractions greener alternative product characteristics Waste Prevention
Learn more about the Principles of Green Chemistry,.storage temp. room temp
Safety Informationpictograms GHS02,GHS06,GHS08 signalword Danger hcodes H225 - H302 + H312 - H315 - H319 - H331 - H336 - H351 - H361d - H370 - H372 pcodes P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P311 - P308 + P311 Target Organs Central nervous system, Eyes RIDADR UN 3316 9 WGK Germany WGK 3 Flash Point F 49.5 °F - closed cup Flash Point C 9.7 °C - closed cup Узнать цену -
Cholesterol Quantitation Kit MAK043-1KT
NACRES: NA.25 General descriptionCholesterol is an important component of mammalian cell membranes where it functions in intracellular transport, cell signaling, and maintaining membrane fluidity. Within the blood, cholesterol circulates as both the free acid and as cholesterol esters. Controlling serum cholesterol has an important therapeutic role as elevated cholesterol levels are associated with the development of atherosclerosis and cardiovascular pathologies. Recent evidence suggests a disturbance of cholesterol homeostasis contributes to the development of a chronic inflammatory state.
Cholesterol is predominantly produced in the liver. Abnormal cholesterol levels are implicated in coronary heart disease. Cholesterol is associated with breast cancer progression or inhibition. Tumor cells are capable of synthesizing their own cholesterol, which aids in cell proliferation. Increased cholesterol level is observed in cancers, especially in prostate cancer. Elevated serum cholesterol is observed in obesity.ApplicationCholesterol Quantitation Kit has been used to measure the total serum cholesterol level.SuitabilitySuitable for use with cell and tissue culture samples, urine, plasma, serum, and other biological samples.PrincipleThe Cholesterol Quantitation Kit can also be used to determine the concentration of free cholesterol, cholesteryl esters, or both (total) present in a sample. In this kit, total cholesterol concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (ex = 535/em = 587 nm) product, proportional to the cholesterol present.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety InformationPictograms GHS08 Signal Word Danger Hazard Statements H317 - H334 Precautionary Statements P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 RIDADR NA 1993 / PGIII WGK Germany WGK 3 Flash Point(F) 188.6 °F - closed cup Flash Point(C) 87 °C - closed cup Узнать цену -
Cholesterol Uptake Assay Kit MAK325-1KT
General descriptionCholesterol is a sterol and lipid present in cell membranes, and is transported in the bloodstream of all animals. It is used to form cell membranes and hormones, and plays important roles in cell signaling processes. Cellular regulation of cholesterol levels is a complex system in which irregularities have been tied to obesity and heart disease. Increased cholesterol uptake has also been linked to highly proliferative cancer cells. Through monitoring cellular cholesterol uptake, one can explore these growing health problems and screen for possible drug treatments.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for testing cholesterol uptake by adherent cells, screening of
cholesterol uptake inhibitors, and evaluation of effect of drugs on
cholesterol uptake.PrincipleThe cholesterol uptake assay kit is based on cellular uptake of a fluorescently tagged cholesterol probe. The fluorescence intensity measured at λex/em = 485/535 nm is proportional to the amount of cholesterol uptaken by the cells.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS02,GHS07,GHS08 signalword Danger hcodes H225 - H319 - H336 - H371 pcodes P210 - P233 - P240 - P241 - P305 + P351 + P338 - P308 + P311 Target Organs Central nervous system, Eyes Узнать цену -
Cholesterol/Cholesteryl Ester Kit II MAK396-1KT
General descriptionCholesterol is an essential molecule in all animal life. It has been involved in both normal development and diseases. The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a colorimetric method. Most of the cholesterol in blood is in the form of cholesteryl ester. These esters can be hydrolyzed to free cholesterol under the appropriate conditions.Features and BenefitsCholesterol/Cholesteryl Ester Kit II:- Simple procedure with results in 1 hour.
SuitabilityThe kit is suitable for the quantification of cholesterol/cholesteryl ester in serum, cells and tissue samples.PrincipleIn the assay, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at A450 nm. The assay can detect free or total cholesterol depending upon whether esterase is utilized to hydrolyze cholesterol esters present. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters). The probe in the kit is more stable, sensitive and specific. The assay can tolerate interferences from various samples significantly.ПараметрыQuality Level 100, detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05 signalword Danger hcodes H314 pcodes P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 Узнать цену -
Choline/Acetylcholine Quantification Kit MAK056-1KT
NACRES: NA.25 General descriptionCholine is an essential nutrient, commonly grouped with the B complex vitamins, that plays key roles in many biological processes. Choline is a precursor for the synthesis of acetylcholine, a critical neurotransmitter in both the peripheral and central nervous systems.ApplicationCholine/Acetylcholine Quantification Kit has been used to quantify the amounts of choline and acetylcholine in samples.SuitabilitySuitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.PrincipleIn this assay, choline concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (ex = 535/em = 587 nm) product, proportional to the choline present. Acetylcholine levels can be determined by adding acetylcholinesterase to the reaction, which hydrolyzes acetylcholine to choline and acetate.Параметрыusage sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay) detection method colorimetric, fluorometric storage temp. −20°C
Safety InformationRIDADR NA 1993 / PGIII Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Chromium Assay Kit MAK130-1KT
NACRES: NA.84 General descriptionChromium, an essential trace element, is a transition metal that primarily exists in two stable oxidation states, hexavalent [Cr(VI)] and trivalent [Cr(III)]. Chromium is widely used in various industries such as electroplating, leather tanning, chrome paint, dying, hardened steel, ceramic, and glass. Cr(VI) is produced almost exclusively by industrial processes; whereas, in nature chromium primarily exists in its trivalent form. Cr(III) is generally regarded as nontoxic due to its poor absorption. Cr(VI) is considered a pulmonary carcinogen and has tested positive in genotoxicity tests. Cr(VI) is considered a serious pollutant in many water streams due to its carcinogenic potential. In trace amounts, Cr(III) may be required for glucose and lipid metabolism.Features and BenefitsCompatible with high-throughput handling systems. Can be adapted for use with cuvettes.SuitabilityThis kit is suitable for the detection of chromium in biological, environmental, food, and beverage samples.PrincipleIn this kit, chromium is determined in a simple, one-step assay in which Cr(VI) forms a specific complex with a chromgenic dye, resulting in a colorimetric (480 nm) product, proportional to the chromium present. Cr(III) can be converted to Cr(VI) with nitric acid/hydrochloric acid, allowing the determination of Cr(III) and/or total chromium [Cr(III) +Cr(VI)].Параметрыusage sufficient for 250 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05,GHS06,GHS08,GHS09 signalword Danger hcodes H290 - H312 - H315 - H318 - H331 - H360D - H410 pcodes P273 - P280 - P302 + P352 + P312 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313 RIDADR UN 3316 9 Узнать цену -
Chymotrypsin Activity Assay Kit MAK345-1KT
General descriptionChymotrypsin is a key serine protease involved in dietary protein digestion in mammals. It is primarily produced by the pancreas, but may be expressed in other tissues, including spleen and liver. In the pancreas, chymotrypsin is initially expressed as the inactive proenzyme chymotrypsinogen, which is cleaved by other proteases to chymotrypsin, its active form. Chymotrypsin specifically cleaves peptide bonds at the C-terminal end of bulky hydrophobic or aromatic amino acids (such as tyrosine, tryptophan or phenylalanine).SuitabilitySuitable for the measurement of chymotrypsin activity in tissue lysate or cultured cellsPrincipleThe Chymotrypsin Activity Assay kit uses a synthetic fluorogenic substrate, enabling kinetic measurement of chymotrypsin activity in cell and tissue lysates. A chymotrypsin activator cleaves chymotrypsinogen to form active chymotrypsin, which then hydrolyzes the non-fluorescent substrate to release a stable fluorophore (Ex/Em = 380/460 nm). The kit includes a selective chymotrypsin inhibitor that can be used to measure specific chymotrypsin activity in samples containing non-specific proteases and endopeptidases that may also metabolize the substrate. The assay can detect as low as 0.01 mU of Chymotrypsin.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS08,GHS09 signalword Danger hcodes H315 - H319 - H334 - H411 pcodes P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
Citrate Assay Kit MAK333-1KT
General descriptionCitrate is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. The Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for citrate determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)PrincipleIn this kit, citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric storage temp. −20°C Узнать цену -
Citrate Assay Kit MAK057-1KT
NACRES: NA.84 General descriptionCitrate is a key Tricarboxylic Acid (TCA) cycle intermediate formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA. Citrate is transported out of the mitochondria via the citrate-malate shuttle and converted back to acetyl-CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis via its actions on acetyl-CoA carboxylase and of glycolysis via its actions on phospho- fructokinase. Citrate metabolism and disposition can vary widely due to sex, age, and a variety of other factors including disease states. Cellular citrate levels are decreased in prostrate cancer cells and citrate levels may be a marker of prostrate cancer growth rate. The Citrate Assay Kit provides a simple, sensitive, and rapid means of quantifying citrate in a variety of samples.ApplicationCitrate Assay kit has been used to determine the concentration of citrate in samples.SuitabilitySuitable for the measurement of citrate in a variety of samples including tissue and cellsPrincipleCitrate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (ex = 535/em = 587 nm) product, proportional to the citrate present. Typical sensitivites of the Citrate Assay Kit are between 0.1 to 10 nmoles (2 µM-10 mM) of citrate in a variety of samples.Параметрыusage sufficient for 100 colorimetric or fluorometric tests detection method colorimetric, fluorometric storage temp. −20°C
Safety Informationpictograms GHS09 hcodes H411 pcodes P273 - P391 - P501 RIDADR NA 1993 / PGIII Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Citrate Synthase Activity Assay Kit MAK193-1KT
EC Number: 232-821-7 NACRES: NA.84 General descriptionCitrate Synthase (CS; EC 2.3.3.1) is a mitochondrial enzyme found in all cells capable of oxidative metabolism. It catalyzes the synthesis of citrate from oxaloacetate in the Krebs tricarboxylic acid cycle.1,2 CS is involved in energy production, lipogenesis, and cholesterologenesis, and its activity follows a circadian pattern.ApplicationCitrate Synthase Activity Assay Kit has been used to measure the activity of the enzyme citrate synthase in samples.SuitabilitySuitable for the detection of citrate synthase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.PrincipleThe Citrate Synthase Activity Assay kit provides a simple and direct procedure for measuring the activity of CS in a variety of tissues, cell cultures (adherent or suspension), and purified mitochondria. CS activity is determined using a coupled enzyme reaction, which results in a colorimetric (412 nm) product proportional to the enzymatic activity present. One unit of CS is the amount of enzyme that generates 1.0 mmole CoA per minute at 25 °C and pH 7.2.Параметрыdetection method colorimetric storage temp. 20°C Gene Information human ... CS(1431)
mouse ... CS(12974)
rat ... CS(170587)
Safety Informationpictograms GHS07 signalword Warning hcodes H319 pcodes P264 - P280 - P305 + P351 + P338 - P337 + P313 RIDADR NONH for all modes of transport Узнать цену -
Citrulline Fluorometric Assay Kit MAK423-1KT
General descriptionL-Citrulline is a non-proteogenic, semi-essential amino acid. Citrulline is formed either by ornithine carbamoyltransferase or as a by-product of the Nitric oxide synthase (NOS) activity. Citrulline generated in the NOS reaction can be recycled to arginine by the two enzymes argininosuccinate synthetase (ASS) and argininosuccinate lyase (ASL) acting in the Urea Cycle. Citrulline concentrations have been shown to be elevated in patients with mutated ASS or ASL genes. Watermelon is one of the richest sources of citrulline. Additionally, citrulline has been advertised as a sports nutrition supplement due to its involvement in Nitric Oxide (NO) synthesis, which helps with vasodilation.Features and BenefitsCitrulline Fluorometric Assay Kit:- Compatible with high-throughput handling systems.
- Sensitive, detecting as low as 2 µM Citrulline in biological samples.
SuitabilityThe kit is suitable for the measurement of citrulline in beverages and biological samples (such as serum) and for the analysis of the urea cycle and NO cycle.PrincipleIn this enzymatic assay, citrulline is converted into a series of intermediates, which further reacts with a probe producing a stable fluorescent signal (ex = 535 nm/em = 587 nm).ПараметрыQuality Level 100, detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 pcodes P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 Узнать цену -
Cobalt Assay MAK024-1KT
NACRES: NA.84 General descriptionCobalt is a transition metal that serves as a trace dietary mineral for all multicellular organisms. Cobalt is an important cofactor for the Vitamin B12 class of compounds where it occupies the center of the vitamin B12 corrin ring. Cobalt can also be coordinated in the active site of the non-corrin containing metalloenzyme methionine aminopeptidase.SuitabilitySuitable for quantitating cobalt concentrations in a variety of samplesPrincipleThe Cobalt Assay kit provides a simple and direct procedure for measuring cobalt in a variety of samples. In this assay, cobalt reacts with 2-mercaptoethanol under basic conditions to form a complex with a strong absorbance at 475 nm. Interference from the metal ions Fe2+, Cu2+, Ni2+, Zn2+, and Mn2+ is <10% at this wavelength. This assay gives a linear range of 10-50 nmoles of cobalt.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. room temp
Safety Informationpictograms GHS08 signalword Warning hcodes H373 - H412 pcodes P273 RIDADR NONH for all modes of transport Узнать цену -
Collagen Assay Kit MAK322-1KT
General descriptionCollagen is the key structural protein of connective tissue and the most abundant protein in mammals. It occurs in many different types and forms with Types I -V being the most common. Aside from the crucial role it plays in the body, it has numerous medical applications such as its use in reconstructive surgery including bone and skin grafts. It is also commonly used in cosmetics due to its anti-aging and skin healing properties.Features and BenefitsCompatible with high-throughput handling systems.SuitabilitySuitable for biological samples (e.g. plasma, serum, cells) and cosmetic samples (e.g. collagen solution)PrincipleAssay methods available for quantifying collagen currently range from needing extensive hydrolysis procedures with acids and bases to using expensive antibodies and complicated protocols. The collagen assay kit delivers a very simple, safe (non-radioactive), and sensitive method to quantify collagen in samples. In the first step of this procedure, collagen in the sample is enzymatically digested into peptides. Subsequently, the N-terminal glycine containing peptides react with the dye reagent to form a fluorescent complex. The fluorescence intensity of this product, measured at λex/em = 375/465 nm, is directly proportional to collagen concentration in the sample.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric
Safety Informationpictograms GHS08 signalword Danger hcodes H334 pcodes P261 - P284 - P501 Узнать цену -
Collagenase Activity Colorimetric Assay Kit MAK293-1KT
NACRES: NA.25 General descriptionCollagenase (EC 3.4.24.3) is an enzyme in the matrix metalloproteinase family that breaks down collagen, assisting in degradation of the extracellular matrix, a key step in the pathogenesis of bacteria. Collagen is an abundant structural protein present in the connective tissue of animals. Collagenase has been used clinically for the treatment of Dupuytrens contracture, an affliction characterized by a thickening of connective tissue.SuitabilitySuitable for the measurement of collagenase activity in bacterial extract and for screening/studying/characterizing collagenase inhibitors in purified enzyme.PrincipleThis Collagenase Activity Assay Kit provides a quick and easy way to determine activity of collagenase. The kit measures collagenase activity using a synthetic peptide (FALGPA) that mimics collagens structure. It is suitable for measuring activity of bacterial collagenases such as from Clostridium histolyticum. In addition, it can also be used to screen/characterize collagenase inhibitors. The limit of detection for this assay is 0.02 mU of collagenase.Параметрыdetection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS02,GHS07,GHS09 signalword Danger hcodes H225 - H302 - H319 - H410 pcodes P210 - P233 - P240 - P273 - P301 + P312 - P305 + P351 + P338 RIDADR UN 3316 9 WGK Germany WGK 3 Узнать цену -
Colorimetric Aldehyde Assay Kit MAK139-1KT
NACRES: NA.84 General descriptionAldehydes are highly reactive molecules, which play a role in many physiological, pathological and commercial processes. They have been implicated as environmental pollutants, and in neurodegeneration and DNA damage. A method for rapid and accurate measurement of aldehydes is an important tool for biological and chemical research, the food industry, and environmental pollution surveillance.
SuitabilityThis kit is Suitable for the detection of aldehydes in a variety of samples, particularly when the samples are at a higher pH. When working with samples in a low pH, product number MAK140, Colorimetric Aldehyde Assay, is more suitable.PrincipleThe Colorimetric Aldehyde Assay Kit provides a simple and direct procedure for measuring aldehydes in a variety of samples. Aldehydes reacts with a dye, resulting in a colorimetric (405 or 550 nm) product proportional to the amount of aldehyde present. The Colorimetric Aldehyde Assay Kit has a lower limit of detection of 1 nanomole of aldehyde in a 100 μL assay volume. This kit has been used for monitoring activities of oxidases that convert an amino group to an aldehyde group.Параметрыusage sufficient for 200 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS05 signalword Danger hcodes H290 - H314 pcodes P234 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 RIDADR NONH for all modes of transport Узнать цену -
Colorimetric Aldehyde Assay Kit MAK140-1KT
General descriptionAldehydes are highly reactive molecules, which play a role in many physiological, pathological and commercial processes. They have been implicated as environmental pollutants, and in neurodegeneration and DNA damage. A method for rapid and accurate measurement of aldehydes is an important tool for biological and chemical research, the food industry, and environmental pollution surveillance.SuitabilityThis kit is suitable for the detection of aldehydes in a variety of samples, particularly when the samples are at a lower pH. When working with samples in a high pH, product number MAK139, Colorimetric Aldehyde Assay, is more suitable.PrincipleThe Colorimetric Aldehyde Assay Kit, Blue provides a simple and direct procedure for measuring aldehydes in a variety of samples. Aldehydes reacts with a sensor, resulting in a colorimetric (620 nm) product proportional to the amount of aldehyde present. The Colorimetric Aldehyde Assay Kit, Blue has a lower limit of detection of 0.3 nanomole of aldehyde in a 100 μL assay volume (3 μM).Параметрыusage sufficient for 200 colorimetric tests detection method colorimetric storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Colorimetric Biotin Assay Kit MAK171-1KT
EC Number: 200-399-3 NACRES: NA.84 General descriptionBiotinylation is the process of labelling a molecule with a biotin molecule to take advantage of the strong binding and high specificity of biotin and streptavidin for purification or detection applications. Biotinylation is typically used to label proteins or nucleic acid based targets for these applications. Quantitation of biotinylation can be used to optimize the labelling of a given target. Levels of biotinylation can effect detection sensitivity or functionality of a purification product.SuitabilitySuitable for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solutionPrincipleThis Colorimetric Biotin Quantitation Kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution. The assay utilizes HABA (4′-hydroxyazobenzene-2-carboxylic acid), a reagent that shows dramatic spectral changes when bound to avidin. Biotin easily displaces HABA from the HABA/Avidin complex, resulting in a decrease of absorption at 500 nm.Preparation NoteStorage and Stability: The kit is shipped on dry ice and storage at –20 °C, protected from light, is recommended.
Precautions and Disclaimer: This product is for R&D;use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.Параметрыdetection method colorimetric shipped in dry ice storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Узнать цену -
Colorimetric Maleimide Assay Kit MAK162-1KT
NACRES: NA.84 General descriptionMaleimide is an unsaturated imide used in protein conjugation reactions. The direct measurement of maleimide moieties following conjugation reactions by spectrophotometric analysis at 302 nm is insensitive due to the small extinction coefficient (620M-1 cm-1) and complicated by protein absorbance at this wavelength.SuitabilitySuitable for quantifying maleimide groupsPrincipleThe colorimetric maleimide quantiation kit provides a simple and direct procedure for measuring maleimide groups. The sample is first reacted with a known amount of excess thiol and then the remaining unreacted thiol is assayed using 4,4′-DTDP (4,4′-Dithiodipyridine). The amount of maleimide is calculated as the difference between the initial amount of thiol and the amount of unreacted thiol after the complete reaction of all maleimide groups.Параметрыusage sufficient for 100 colorimetric tests detection method colorimetric storage temp. −20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H302 - H315 - H319 - H335 pcodes P261 - P305 + P351 + P338 Target Organs Respiratory system RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Colorimetric Sphingomyelinase Assay Kit MAK152-1KT
NACRES: NA.84 General descriptionSphingomyelinase (SMase), or sphingomyelin phosphodiesterase, catalyzes the hydrolytic cleavage of sphingolipid to phosphocholine and ceramide, a second messenger involved in multiple cellular responses including the cellular response to stress. There are at least 5 types of SMases identified which are classified according to their cation dependence, cellular location, and pH optima for activity and include the acidic and neutral sphingomyelinase. Neutral SMases, along with the acidic SMases, are thought to be major contributors to the production of ceramide in response to cellular stress.Features and BenefitsCompatible with high-throughput handling systems.SuitabilityThis kit is suitable for the detection of sphingomyelinase activity in a variety of samples such as blood and cell extracts or for screening sphingomyelinase inhibitors and activators.PrincipleThe Sphingomyelinase Assay Kit provides a simple and direct procedure for measuring neutral SMase activity in a variety of samples such as blood or cell extracts, or for screening SMase activators and inhibitors. SMase activity is determined by a coupled enzyme assay, which results in a colorimetric (655 nm) product proportional to the SMase activity present.Параметрыusage sufficient for 200 colorimetric tests detection method colorimetric storage temp. 20°C Gene Information human ... SMPD2(6610), SMPD3(55512), SMPD4(55627)
mouse ... SMPD2(20598), SMPD3(58994), SMPD4(77626)
rat ... SMPD2(83537), SMPD3(94338), SMPD4(303790)
Safety Informationpictograms GHS05,GHS08,GHS09 signalword Danger hcodes H317 - H318 - H334 - H410 pcodes P261 - P273 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 RIDADR NONH for all modes of transport Узнать цену -
Copper Assay Kit MAK127-1KT
NACRES: NA.84 General descriptionCopper is an essential trace element. Copper-containing enzymes play important roles in iron and catecholamine metabolism, free radical scavenging, and in the synthesis of hemoglobin, elastin, and collagen. Copper is mainly present in ceruloplasmin in the liver. Low levels of copper have been associated with mental retardation, depigmentation, anemia, hypotonia, and scorbutic changes in bone. Levels of copper are key diagnostic indicator of diseases such as Wilson′s disease, microcytic hypochromic anemia, and bone disease due to reduced collagen synthesis.ApplicationCopper Assay Kit has been used to determine the copper content colorimetrically in samples.Features and BenefitsCompatible with high-throughput handling systems. Can be adapted for use with cuvettes.SuitabilitySuitable for the detection of of copper in biological, environmental, food, and beverage samples. Suitable for studying the effects of compounds on copper metabolism.PrincipleThe method utilizes a chromogen that forms a colored complex specifically with copper ions. The intensity of the color, measured colorimetrically (359 nm), is directly proportional to copper concentration in the sample. The range of linear detection is 7 μg/dL (1.0 μM) to 300 μg/dL (47 μM).Параметрыusage sufficient for 250 colorimetric tests detection method colorimetric storage temp. 2-8°C
Safety Informationpictograms GHS05,GHS07,GHS09 signalword Danger hcodes H290 - H314 - H335 - H410 pcodes P234 - P261 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338 Target Organs Respiratory system RIDADR UN 3316 9 WGK Germany WGK 3 Узнать цену
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