16:0-12 Doxyl PC 810600P-1MG
Empirical Formula (Hill Notation): | C46H90N2O10P |
CAS Number: | 217499-54-2 |
Molecular Weight: | 862.19 |
NACRES: | NA.25 |
Phosphatidylcholine (PC) is a strong bilayer-forming lipid. It the most common phospholipid in mammalian membranes. It is also an important component of the mucosal layer of the colon. 1-palmitoyl-2-stearoyl-(12-doxyl)-
sn-glycero-3-phosphocholine (12-NO-PC) is a nitroxide-labeled phospholipid.
Avantis nitroxide spin product listing is a group of compounds designed to act as membrane probes. A variety of positions down the hydrophobic chain are labeled with the nitroxide functional groups to allow probing the membrane at various depths. These compounds have been synthesized from 1-palmitoyl-2-hydroxy-
sn-glycerol-3-phosphocholine with the product being purified by column chromatography. Various
n-doxyl phosphocholines have been recently used as biophysical tools to elucidate membrane trafficking with phosphatidylinositol transfer proteins and as fluorescent quenchers in lipid bilayer structural studies.
16:0-12 Doxyl PC is suitable for use:
- as a lipophilic collisional quencher to prepare liposomes used in lipophilic quenching experiments
- to prepare liposomes used in nitroxide quenching experiments to examine the location of each NBD (7-nitrobenz-2-oxa-1,3-diazole) probe in mutants
- as a component in POPC or 1:1 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)/1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-rac-1-glycerol (POPG) mixtures to prepare unlabelled large unilamellar liposomes
Phosphatidylcholine (PC) functions as a surfactant within the mucus to form a hydrophobic surface to inhibit bacterial penetrance. It is used to treat fat embolism. Phosphatidylcholine lowers the levels of cholesterol and triglycerides.
5 mL Clear Glass Sealed Ampule (810600P-1mg)
To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of
n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies. For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 µl absolute ethanol for a concentration of 40.3 mM. href="https://pubs.acs.org/doi/suppl/10.1021/ja804929m/suppl_file/ja804929m_si_001.pdf"target="_blank">Supplemental information
assay | >99% (TLC) |
form | powder |
packaging | pkg of 1 x 1 mg (810600P-1mg) |
Торговая марка | Avanti Polar Lipids |
shipped in | dry ice |
storage temp. | 20°C |
RIDADR | NONH for all modes of transport |