Attachment Factors
- Сортировать:
- Вид таблицей
-
Thrombospondin-1 human
NACRES: NA.75
Кат. номер ECM002-50UG ECM002-50UG-PW General descriptionRecombinant human thrombospondin-1 is expressed in HEK293 cells as a glycoprotein with a calculated moleculaer mass of 127.5 kDa. This protein is manufactured in human cells using an all-human production system, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture.ApplicationIn rat C6 astroglioma cells, human thrombospondin 1 has been used in coating the cell culture plates to study the effects of integrins on CNTF (ciliary neurotrophic factor) expression. It has also been used in the preparation of secreted factor cocktail for analysis of the human mesenchymal stem cell secretome.
Recommended for use as a cell culture substratum at 1-5 µg/cm2 or 0.1-25 µg/ml. Optimal concentration depends on cell type as well as the application or research objectives.
Physical form
TSP1 (thrombospondin 1) is believed to play a role in cell migration and proliferation, during embryogenesis and wound repair. TSP1 expression is highly regulated by different hormones and cytokines and is developmentally controlled. TSP1 stimulates the growth of vascular smooth muscle cells and human foreskin fibroblasts. A combination of IFN-γ (interferon γ) and TNF-α (tumor necrosis factor α) inhibits TSP1 production in these cells. In endothelial cells, it controls adhesion, migration as well as proliferation. It also exhibits antiangiogenic properties and regulates immune processes. TSP1 binds to various cell surface receptors, such as integrins and integrin-associated protein CD47. It also plays a crucial role in inflammatory processes and post-inflammatory tissue dynamics. TSP1 has been used as a potential regulator of tumor growth and metastasis. It is upregulated in rheumatoid synovial tissues and might be associated with rheumatoid arthritis. Variants of this gene might be linked with increased risk of autism.
Supplied as a powder, lyophilized from phosphate buffered saline.
Analysis Note
The biological activity of recombinant human thrombospondin-1 was tested in culture by measuring the ability of immobilized DTT-treated thrombospondin-1 to support adhesion of SVEC4-10 cells.ПараметрыQuality Level 200 biological source human recombinant expressed in HEK 293 cells sterility sterile assay 95% (SDS-PAGE) form lyophilized powder mol wt 127.5 kDa (The protein migrates as a 140 kDa band on SDS-PAGE due to glycosylation.) packaging pkg of 50 µg application(s) cell culture | mammalian: suitable impurities 1 EU/µg endotoxin, tested UniProt accession no. P07996, shipped in dry ice storage temp. 20°C Gene Information human ... THBS1(7057)
Safety Informationhcodes H412 pcodes P273 - P501 WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
VitroCol® 5008-15MG
EC Number: 232-697-4 General descriptionVitroCol® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblasts to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.
VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. It contains high monomer content as measured by gel permeation chromatography.
VitroCol® is especially ideal for human cell culture systems when coating of surfaces and providing preparations of thin layers of culturing cells. It is not recommend for the formation of a solid gel. VitroCol® is supplied as a sterile, lyophilized powder.Preparation NoteThis product is supplied as a lyophilized powder with 15 mg of human collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.Legal InformationVitroCol is a registered trademark of Advanced BioMatrix, Inc.ПараметрыQuality Level 100 biological source protein from human sterility sterile-filtered form lyophilized packaging pkg of 15 mg Торговая марка Advanced BioMatrix application(s) cell culture | mammalian: suitable impurities <1 EU/mL Endotoxin level shipped in wet ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Vitronectin 5051-0.1MG
NACRES: NA.75 General descriptionVitronectin is a 75 kDa glycoprotein produced by hepatocytes and liberated into the blood. It has four domains and at least two structural motifs. Vitronectin gene is small with three kilobases in length and has eight exons. It is also present on fibers in the extracellular matrices of several tissues.
Vitronectin is a monomeric glycoprotein used to promote cell attachment, migration, proliferation and differentiation in a broad number of cell lines and types. This product has been purified from human plasma where it is found as a mixture of 75kDa and 65kDa polypeptides.
Vitronectins primary use in cell culture is related to cell adhesion. It also binds to heparin and collagen.
Vitronectin is ideal for coating of surfaces. The optimal concentration for cell attachment and culture may differ for various cell types. Vitronectin has been used at a final coating concentration as low as 50 ng/cm2 on plasticware. It is provided in user-friendly packaging for use and storage. Vitronectin is sterile filtered and is supplied as a ready to use solution after thawing and concentration adjustment.
Biochem/physiol Actions
Vitronectin controls proteolysis. It helps in in vitro cell adhesion.Vitronectin is involved in the modulation of perforin mediated killing by cytotoxic T-lymphocytes and natural killer (NK) cells. It controls the inflammatory reactions in blood.
Preparation Note
This product is supplied in a solution of 0.15 M NaCl and 0.005M HEPES at pH 7.4ПараметрыQuality Level 100 biological source human plasma volume size 0.2 mL sterility sterile-filtered assay >95% form solution mol wt 75 kDa packaging pkg of 0.1 mg concentration 0.5 mg/mL application(s) cell culture | mammalian: suitable solubility water: miscible UniProt accession no. P04004, shipped in dry ice storage temp. 20°C Gene Information human ... VTN(7448)
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Vitronectin from bovine plasma
CAS Number: 83380-82-9 MDL number: MFCD00132575 NACRES: NA.75
Кат. номер V9881-50UG V9881-50UG-PW ApplicationVitronectin can be used with cells with integrin receptors that bind vitronectin, including platelets, endothelial cells, melanoma cells, and osteosarcoma. It is recommended as a cell culture substratum at 0.1 µg/cm2. Optimal conditions for attachment depend on cell line and application.
Biochem/physiol Actions
Vitronectin binds to glycosaminoglycans, is incorporated as an inhibitor to the membrane cytolytic attack complex of the complement system, has interaction with thrombin and antithrombin III during coagulation, and may play a role in the coagulation pathway.
Components
Vitronectin is a glycoprotein present in plasma and tissues, and is one of the major cell adhesion proteins, along with fibronectin, in plasma. The N-terminal residues of vitronectin are identical to somatomedin B and are followed by an R-G-D sequence that interacts with a specific cell-surface receptor. The central domain of vitronectin is enriched with hydrophobic residues. Closer to the C-terminus, it has an arginine rich region that allows for heparin binding activity.
Caution
The lyophilized product should be stored desiccated at 2-8°C. Under these conditions it is stable for at least 2 years. Aliquots should be stored at -20°C or -70°C, but repeated freezing and thawing should be avoided.
Preparation Note
This product is supplied as a cell culture tested lyophilized powder from buffered saline. It is recommended to use polypropylene vessels during handling, due to the strong affinity of vitronectin for hydrophilic surfaces. Reconstitution of this product can be done with 1 mL of water per vial. The stock solution should then be filter-sterilized using a 0.2μm membrane and aliquoted and frozen for long term storage.Параметрыbiological source bovine plasma Quality Level 200 sterility non-sterile product line BioReagent form lyophilized powder packaging pkg of 50 µg application(s) cell culture | mammalian: suitable surface coverage 0.1 µg/cm2 solubility water: soluble 1.0 mL, clear, colorless UniProt accession no. Q3ZBS7, shipped in ambient storage temp. 2-8°C Gene Information bovine ... VTN(507525)
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Vitronectin from human plasma
CAS Number: 83380-82-9 MDL number: MFCD00132575 NACRES: NA.75
Кат. номер V8379-50UG-PW V8379-500UG V8379-50UG
Packaging
50 μg in glass bottleApplicationVitronectin can be used with cells with integrin receptors that bind vitronectin, including platelets, endothelial cells, melanoma cells, and osteosarcoma. It is recommended as a cell culture substratum at 0.1 µg/cm2. Optimal conditions for attachment depend on cell line and application.
Biochem/physiol Actions
Vitronectin binds to glycosaminoglycans, is incorporated as an inhibitor to the membrane cytolytic attack complex of the complement system, has interaction with thrombin and antithrombin III during coagulation, and may play a role in the coagulation pathway.
Components
Vitronectin is a glycoprotein present in plasma and tissues, and is one of the major cell adhesion proteins, along with fibronectin, in plasma. The N-terminal residues of vitronectin are identical to somatomedin B and are followed by an R-G-D sequence that interacts with a specific cell-surface receptor. The central domain of vitronectin is enriched with hydrophobic residues. Closer to the C-terminus, it has an arginine rich region that allows for heparin binding activity.
Caution
The lyophilized product should be stored desiccated at 2-8°C. Under these conditions it is stable for at least 2 years. Aliquots should be stored at -20°C or -70°C, but repeated freezing and thawing should be avoided.
Preparation Note
This product is supplied as a cell culture tested lyophilized powder from buffered saline. It is recommended to use polypropylene vessels during handling, due to the strong affinity of vitronectin for hydrophilic surfaces. Reconstitution of this product can be done with 1 mL of water per vial. The stock solution should then be filter-sterilized using a 0.2μm membrane and aliquoted and frozen for long term storage.ПараметрыQuality Level 300 biological source human plasma sterility non-sterile product line BioReagent form lyophilized powder mol wt A subunit 75 kDa","B subunit 65 kDa packaging pkg of 50 µg application(s) cell culture | mammalian: suitable surface coverage 0.1 µg/cm2 solubility water: soluble 0.050 mg/mL, clear, colorless UniProt accession no. P04004, shipped in ambient storage temp. 2-8°C Gene Information human ... VTN(7448)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Vitronectin from rat plasma
CAS Number: 83380-82-9 MDL number: MFCD00132575 NACRES: NA.75
Кат. номер V0132-50UG V0132-50UG-PW General descriptionVitronectin, also known as epibolin and S protein, is a 75 kDa protein. It consists of three glycosylation sites. Vitronectin is mainly expressed in plasma.ApplicationVitronectin has been used as a constituent of serum free media for culturing skeletal muscle and for growth and myelination of motoneurons by Schwann cells. It has also been used as a constituent of enriched co-culture media for culturing rat spinal cord.
Vitronectin can be used with cells with integrin receptors that bind vitronectin, including platelets, endothelial cells, melanoma cells, and osteosarcoma. It is recommended as a cell culture substratum at 0.1 µg/cm2. Optimal conditions for attachment depend on cell line and application.
Biochem/physiol Actions
Vitronectin inhibits the membrane cytolytic attack complex of the complement system. It interacts with glycosaminoglycans, collagen, plasminogen and the urokinase-receptor. Vitronectin binds to heparin, thrombin and antithrombin III during coagulation and may play a role in the coagulation pathway. It acts a connector between cell adhesion and physiological proteolysis. Vitronectin in association with integrins αvβ3, αvβ5, αvβ1, αIIbβ3, αvβ6 and αvβ8 stimulates cell adhesion, spreading and migration.
Components
Vitronectin is a glycoprotein present in plasma and tissues, and is one of the major cell adhesion proteins, along with fibronectin, in plasma. The N-terminal residues of vitronectin are identical to somatomedin B and are followed by an R-G-D sequence that interacts with a specific cell-surface receptor. The central domain of vitronectin is enriched with hydrophobic residues. Closer to the C-terminus, it has an arginine rich region that allows for heparin binding activity.
Caution
The lyophilized product should be stored desiccated at 2-8°C. Under these conditions it is stable for at least 2 years. Aliquots should be stored at -20°C or -70°C, but repeated freezing and thawing should be avoided.
Preparation Note
This product is supplied as a cell culture tested lyophilized powder from buffered saline. It is recommended to use polypropylene vessels during handling, due to the strong affinity of vitronectin for hydrophilic surfaces. Reconstitution of this product can be done with 1 mL of water per vial. The stock solution should then be filter-sterilized using a 0.2μm membrane and aliquoted and frozen for long term storage.ПараметрыQuality Level 200 biological source rat plasma sterility non-sterile product line BioReagent form lyophilized powder mol wt 75 kDa packaging pkg of 50 µg application(s) cell culture | mammalian: suitable surface coverage 0.1 µg/cm2 solubility water: soluble 1.00 mL, clear, colorless UniProt accession no. Q3KR94, shipped in dry ice storage temp. 2-8°C Gene Information rat ... Vtn(29169)
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Vitronectin human
CAS Number: 83380-82-9 NACRES: NA.32
Кат. номер SRP3186-250UG SRP3186-250UG-PW General descriptionVitronectin is a secreted glycoprotein which is synthesized in the liver. It is also secreted by respiratory epithelial cells and is present in healthy lungs. It circulates primarily in monomeric form, but can undergo conformational change to a structure that forms disulfide linked multimers. Recombinant human Vitronectin is a 459 amino acid single chain monomeric protein, which migrates at an apparent molecular weight of 75kDa by SDS-PAGE under reducing conditions. Recombinant vitronectin can function as a chemically defined matrix component in human embryonic stem cell renewal media.ApplicationVitronectin human has been used to study the effects of ECM (extracellular matrix) ligands on C6 astroglioma cells. It has also been used as an attachment-promoting protein for coating the cell plates.
Biochem/physiol Actions
The multimeric Vitronectin can efficiently bind to and incorporate into the extracellular matrix. Within the matrix, Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Its interaction with integrin v3 enhances hypoxia resistance and tumor cell invasion. It is also associated with cancer progression in various cancers. Vitronectin is involved in wound repair and also controls complement system. It works as a marker of profibrotic activity in liver, heart and kidney.
Sequence
DQESCKGRCT EGFNVDKKCQ CDELCSYYQS CCTDYTAECK PQVTRGDVFT MPEDEYTVYD DGEEKNNATV HEQVGGPSLT SDLQAQSKGN PEQTPVLKPE EEAPAPEVGA SKPEGIDSRP ETLHPGRPQP PAEEELCSGK PFDAFTDLKN GSLFAFRGQY CYELDEKAVR PGYPKLIRDV WGIEGPIDAA FTRINCQGKT YLFKGSQYWR FEDGVLDPDY PRNISDGFDG IPDNVDAALA LPAHSYSGRE RVYFFKGKQY WEYQFQHQPS QEECEGSSLS AVFEHFAMMQ RDSWEDIFEL LFWGRTSAGT RQPQFISRDW HGVPGQVDAA MAGRIYISGM APRPSLAKKQ RFRHRNRKGY RSQRGHSRGR NQNSRRPSRA TWLSLFSSEE SNLGANNYDD YRMDWLVPAT CEPIQSVFFF SGDKYYRVNL RTRRVDTVDP PYPRSIAQYW LGCPAPGHL
Physical form
Lyophilized from Phosphate Buffered Saline (PBS), pH 7.4, plus 2.5% trehalose.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.ПараметрыQuality Level 200 biological source human recombinant expressed in HEK 293 cells sterility sterile assay 95% (HPLC)","95% (SDS-PAGE) form lyophilized potency 0.5 µg/mL EC50 mol wt 75 kDa packaging pkg of 250 µg application(s) cell culture | mammalian: suitable impurities <0.1 EU/µg endotoxin, tested color white UniProt accession no. P04004, shipped in wet ice storage temp. 20°C Gene Information human ... VTN(7448)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Vitronectin, Human Purified Protein CC080
eCl@ss: 32160405 NACRES: NA.75 General descriptionProduct Source: Human plasma; tested negative for antibodies to HBsAg and HIV.
PROPERTIES:
Binds to heparin. Active in cell attachment of MG63 cells.
Vitronectin (S-protein) is a multi-functional plasma and extracellular matrix protein with defined activity in cell adhesion, thrombosis, complement activation, fibrinolysis, inflammation, and platelet adhesion. Vitronectin binds to other macromolecules and participates in regulatory circuits to control these processes. Some target macromolecules that interact with vitronectin are heparin, PAI-1, proteases such as thrombin and urokinase-type plasminogen activator (uPA), serine protease inhibitor-protease complexes, the uPA receptor, and a sub-class of integrin receptors on the surface of cells. Vitronectin is also applied as a thin coating on tissue-culture surfaces, which is useful to promote cell attachment, spreading, proliferation, and differentiation of many normal and neoplastic cells, and to study cell migration.ApplicationSuitable for use in ELISA, immunization, cell attachment assays and electrophoresis.LinkageReplaces: 08-126
Physical form
Liquid in 50 mM sodium phosphate, 100 mM NaCl, pH 7.4, containing no preservatives.
Storage and Stability
Stable 12 months at -70°C. Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the containers cap.
Analysis Note
Two bands in SDS-PAGE at 65 kDa and 75 kDa.Legal InformationCHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 100 biological source human assay 90% (SDS-PAGE) form liquid specific activity 1 µg/mL (cell attachment assays) Торговая марка Chemicon® concentration 1 mg/mL application(s) cell culture | mammalian: suitable input sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)NCBI accession no. NM_000638.3, UniProt accession no. P04004, shipped in dry ice storage temp. 70°C Gene Information human ... VTN(7448)
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Vitronectin, human recombinant 8-126
eCl@ss: 32160405 NACRES: NA.75 General descriptionProduct Source: N-terminal, GST-tagged, full-length human Vitronectin expressed in E. coli
Quality
Routinely evaluated by cell adhesion assay.LinkageReplaces: CC080
Physical form
Liquid in 10mM Sodium Phosphate, pH 7.1, 150mM Sodium Chloride, 0.1mM DTT, 0.1mM PMSF and 50% glycerol.
Storage and Stability
6 months at -20°C from date of shipmentLegal InformationUPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 100 biological source human form liquid mol wt Mw 78 kDa Торговая марка Upstate® concentration 1.5 mg/mL application(s) cell culture | mammalian: suitable input sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)NCBI accession no. NM_000638.3, UniProt accession no. P04004, shipped in dry ice storage temp. 20°C Gene Information human ... VTN(7448) Узнать цену
Получите коммерческое предложение в течение 1 часа
Менеджер подготовит коммерческое предложение и позвонит, если понадобится уточнить детали вашего заказа
Анна Гловацкая
Менеджер по работе с клиентами
С 2010 года мы поставляем оборудование с заводов Европы. Берем на себя все — от подбора оборудования до внедрения на предприятии
Все сотрудники имеют высшее образование, закончили ведущие химические вузы страны, такие как РХТУ им Менделеева.
У большинства компаний срок ожидания составляет 10-12 недель.
Оборудование хранится на сухом отапливаемом складе, где поддерживается ровная температура.
Работаем с PonyExpress и Деловыми линиями. Вы также можете выбрать свою транспортную компанию или забрать товар со склада в Москве.
В случае любых неполадок за свой счет выполним ремонт в сервисном центре или на заводе-изготовителе. Или бесплатно заменим прибор на новый.
Производим пуско-наладку оборудования, валидацию, обучение сотрудников. Если нужно, привлекаем инженеров с заводов- изготовителей.
