Thrombospondin-1 human

NACRES:

NA.75

Кат. номер

ECM002-50UG

ECM002-50UG-PW

Recombinant human thrombospondin-1 is expressed in HEK293 cells as a glycoprotein with a calculated moleculaer mass of 127.5 kDa. This protein is manufactured in human cells using an all-human production system, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture.
In rat C6 astroglioma cells, human thrombospondin 1 has been used in coating the cell culture plates to study the effects of integrins on CNTF (ciliary neurotrophic factor) expression. It has also been used in the preparation of secreted factor cocktail for analysis of the human mesenchymal stem cell secretome.
Recommended for use as a cell culture substratum at 1-5 µg/cm2 or 0.1-25 µg/ml. Optimal concentration depends on cell type as well as the application or research objectives.
Physical form
TSP1 (thrombospondin 1) is believed to play a role in cell migration and proliferation, during embryogenesis and wound repair. TSP1 expression is highly regulated by different hormones and cytokines and is developmentally controlled. TSP1 stimulates the growth of vascular smooth muscle cells and human foreskin fibroblasts. A combination of IFN-γ (interferon γ) and TNF-α (tumor necrosis factor α) inhibits TSP1 production in these cells. In endothelial cells, it controls adhesion, migration as well as proliferation. It also exhibits antiangiogenic properties and regulates immune processes. TSP1 binds to various cell surface receptors, such as integrins and integrin-associated protein CD47. It also plays a crucial role in inflammatory processes and post-inflammatory tissue dynamics. TSP1 has been used as a potential regulator of tumor growth and metastasis. It is upregulated in rheumatoid synovial tissues and might be associated with rheumatoid arthritis. Variants of this gene might be linked with increased risk of autism.
Supplied as a powder, lyophilized from phosphate buffered saline.
Analysis Note
The biological activity of recombinant human thrombospondin-1 was tested in culture by measuring the ability of immobilized DTT-treated thrombospondin-1 to support adhesion of SVEC4-10 cells.

Quality Level	200
biological source	human
recombinant	expressed in HEK 293 cells
sterility	sterile
assay	95% (SDS-PAGE)
form	lyophilized powder
mol wt	127.5 kDa (The protein migrates as a 140 kDa band on SDS-PAGE due to glycosylation.)
packaging	pkg of 50 µg
application(s)	cell culture | mammalian: suitable
impurities	1 EU/µg endotoxin, tested
UniProt accession no.	P07996,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... THBS1(7057)

hcodes	H412
pcodes	P273 - P501
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable