Poly-D-lysine hydrobromide

Linear Formula:	D-Lys-(D-Lys)n-D-Lys · xHBr
CAS Number:	27964-99-4
MDL number:	MFCD00131934
PubChem Substance ID:	24898764
NACRES:	NA.75

Кат. номер

P6407-5MG-PW

P6407-PH

P6407-5MG

P6407-10X5MG

5, 10×5 mg in glass bottle
Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.
This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.
This product is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.
Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.
Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.
This product has a molecular weight of 70,000-150,000 and is cell culture tested and gamma-irradiated. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Quality Level	200
sterility	-irradiated
product line	BioReagent, BioXtra
form	lyophilized powder
mol wt	70,000-150,000
packaging	pkg of 10 X 5 mg, pkg of 5 mg
application(s)	cell culture | mammalian: suitable
solubility	H2O: soluble 50 mg/mL, clear, colorless
shipped in	ambient
storage temp.	20°C
SMILES string	O=C(C)[C@@](NC)([H])CCCCN.[Br]
InChI	1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H
InChI key	MEXAGTSTSPYCEP-UHFFFAOYSA-N

Personal Protective Equipment	Eyeshields, Gloves, type N95 (US)
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable