PluriSTEM-XF® Recombinant Vitronectin CC130

eCl@ss:	32160405
NACRES:	NA.75

PluriSTEM Recombinant Vitronectin is an effective xeno-free alternative to matrigel and has been validated to successully culture human ES and iPS cells using PluriSTEM Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media.
PluriSTEM Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.
Research Category
Stem Cell Research
Cell Structure
ECM & Adhesion
Supports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions.
Quality
Each lot is tested on human ES cells (H1) cultured using 5-10 μg recombinant vitronectin in 1 mL human ESC medium per well (6-well plate).
Physical form
Product is sterile-filtered in a 20 mM pH 8.0 Tris-HCl buffer, with a proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and glycerol.
Storage and Stability
Store at -20°C until expiration date on vial. Once thawed, product is stable at 2 - 8°C for two weeks. Thawed vitronectin can be aliquoted and stored at -20°C. Avoid additional freeze-thaw cycles.
PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source	Escherichia coli","human
Quality Level	100
assay	95% (SDS-PAGE)
Торговая марка	PluriSTEM®
concentration	0.5 mg/mL
application(s)	cell analysis: suitable (cell attachment)","cell culture | mammalian: suitable","cell culture | stem cell: suitable
input	sample type: human embryonic stem cell(s)
UniProt accession no.	P04004,
shipped in	dry ice

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable