Human Collagen Type II CC052

eCl@ss:	32160405
NACRES:	NA.75

Collagen type II was purified by using differential salt precipitation, alcohol precipitation and DEAE chromatography. Multiple purification steps provide final preparations of collagen types in the native triple helical form, free of any non-collagenous protein. Contamination of other collagen types in a standard preparation is lower then five percent totally.
COL2A1 is the gene responsible for the production of the alpha1(II) chain of type II collagen. Type II collagen, which adds structure and strength to connective tissues, is found primarily in cartilage, the gel that fills the eyeball (vitreous body), inner ear, and the center portion of the discs between the vertebrae in the spine (nucleus pulposus).
There are two forms of type II collagen made in the body. One version, type IIA, is made mainly in the vitreous body of the eye. The second version, type IIB, is preferentially produced in adult cartilage tissue.
Collagen II is initially produced as type II procollagen, a protein consisting of three pro-alpha1(II) chains twisted together to form a triple-stranded helical (spiral-shaped) molecule. While in the cell, enzymes modify certain amino acids (the building blocks of proteins), specifically lysine and proline, by adding chemical groups that are necessary for the strands to come together in a stable structure and then cross-link with other molecules. Other enzymes add sugars to the protein. The triple-stranded type II procollagen molecule leaves the cell and is converted to collagen by enzymes that clip small segments off both ends. The collagen molecules arrange themselves into long, thin fibrils outside of the cell. The fibrils come together in side-by-side groups to form collagen fibers. Cross-linking between molecules in fibrils produces a very stable protein structure, which contributes to collagens tissue-strengthening function.{http://ghr.nlm.nih.gov}
Physical form
Purified protein. Liquid in 0.5M acetic acid. No preservative.
Storage and Stability
Aliquot and freeze. Maintain at -20°C up to 12 months. Avoid repeated freeze/thaw cycles.
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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	human
assay	90% (Human collagen type II)
form	liquid
Торговая марка	Chemicon®
concentration	1 mg/mL
application(s)	cell culture | mammalian: suitable
impurities	<0.5% Non-collagen proteins","<1% Human collagen type IV","<1% Human collagen type V","<10% Human collagen type I","<10% Human collagen type III
input	sample type induced pluripotent stem cell(s) sample type mesenchymal stem cell(s) sample type: human embryonic stem cell(s) sample type neural stem cell(s) sample type hematopoietic stem cell(s) sample type epithelial cells sample type pancreatic stem cell(s)
solubility	water: soluble at 20 °C
NCBI accession no.	NM_001844.4,","NM_033150.2,
UniProt accession no.	P02458,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... COL2A1(1280)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable