Human Collagen Type I CC050

eCl@ss:	32160405
NACRES:	NA.75

COL1A1 is the gene responsible for the production of the alpha1(I) chain of type I collagen. Collagen, which adds structure and strength to connective tissues, is found throughout the body for example, in skin, tendon, cartilage, ligaments, bone, the part of the eyeball that is white (sclera), and the spaces between cells and tissues called the extracellular matrix.
Type I collagen is initially produced as procollagen in cells. This protein consists of two pro-alpha1(I) protein strands combined with a pro-alpha2(I) procollagen strand that form a triple-stranded rope-like structure. While in the cell, enzymes modify certain amino acids in the protein (lysine and proline) by adding chemical groups that are necessary for the three strands to form stable molecules and make connections (cross-links) between chains. Other enzymes add sugars to the protein. Now complete, the triple-stranded type I procollagen molecule leaves the cell and is processed by enzymes that clip small segments off both ends. The procollagen molecules arrange themselves into long, thin fibrils outside of the cell. The fibrils come together in side-by-side groups to form collagen fibers. Cross-linking between molecules in fibrils produces a very stable protein structure, which contributes to collagens tissue strengthening function. {http://ghr.nlm.nih.gov}
Human type I collagen purified by serial salt precipitations, alcohol precipitation and DEAE chromatography of a pepsin extraction of human placenta. Composition: [1(I)]2, <2(I), native triple helix.
Physical form
Purified protein. Liquid containing 0.5 M Acetic acid, pH 2.5. Can be diluted in PBS for applications.
Analysis Note
Purity was controlled by SDS-PAGE and reaction with anti-collagen type-specific antibodies
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Quality Level	100
biological source	human
assay	>90% (collagen type I, SDS-PAGE)
form	liquid
Торговая марка	Chemicon®
concentration	1 mg/mL
application(s)	cell culture | mammalian: suitable
impurities	<1% collagen type II,IV-VI & non-collagen proteins.","<10% collagen type III
input	sample type mesenchymal stem cell(s) sample type induced pluripotent stem cell(s) sample type neural stem cell(s) sample type: human embryonic stem cell(s) sample type epithelial cells sample type pancreatic stem cell(s) sample type hematopoietic stem cell(s)
solubility	water: soluble at 20 °C
NCBI accession no.	NM_000088.3,
UniProt accession no.	P02452,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... COL1A1(1277)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable