- Производитель:
- Sigma-Aldrich
EC Number: | 289-149-2 |
MDL number: | MFCD00131062 |
eCl@ss: | 42020141 |
Кат. номер |
F0556-100UL-PW |
F0556-100UL |
Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35.
- It is used in purification of human extracellular matrix (ECM)
- Coating of tissue culture plates with extracellular matrix components
- Used during cell culture (For xeno-free, feeder-free culture the cells were passaged in to human fibronectin along with other components)
Fibronectin from human plasma can be used with epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1-5 µg/cm2 or 0.5-50 µg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.
Biochem/physiol Actions
Fibronectin is a high molecular mass extracellular matrix glycoprotein involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, cell migration/adhesion, and embryogenesis and development. It is used in vitro as a substrate to enhance adherence and proliferation of many cell types. It has the capacity to bind to macromolecules such as collagen, fibrin, glycosaminoglycans, and Clq, apart from some bacteria.
Components
Fibronectin is an adhesive glycoproteins found in both the cell surface and in plasma.
Caution
The solution should be stored at -20°C. Under these conditions, the product is stable for at least 2 years. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.
Preparation Note
This product is produced by human fibroblasts and then purified biochemically. It is supplied as a sterile solution at 0.5 mg/mL in a CAPS saline buffer. Before use, the product should be thawed slowly at 2-8°C, forming a clear solution. Avoid any vortexing or vigorous shaking of the solution, which will cause any fibronectin to “crash” out of solution. To coat plates, dilute this product in sterile HBSS solutions and coat with a minimal volume. Then, incubate at 37°C for 1-2 hours and wash 3 times with sterile HBSS and plate cells.
Quality Level | 200 |
biological source | human fibroblasts |
sterility | sterile-filtered |
product line | BioReagent |
form | solution |
packaging | pkg of 100 µL |
concentration | ~0.5 mg/mL |
application(s) | cell culture | mammalian: suitable |
UniProt accession no. | P02751, |
shipped in | dry ice |
storage temp. | 20°C |
Gene Information | human ... FN1(2335) |
Personal Protective Equipment | Eyeshields,, Gloves,, type N95 (US), |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point F | Not applicable |
Flash Point C | Not applicable |
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