Collagen, Type I solution from rat tail

CAS Number:	9007-34-5
EC Number:	232-697-4
MDL number:	MFCD00130825
NACRES:	NA.75

Кат. номер

C3867-1VL-PW

C3867-1VL

C3867-PH

Collagen is an extracellular matrix protein comprising triple helical structure. Homotrimetric collagens have three identical α-chains. Type I collagen have one α-chain different in sequence among the three. Type I collagen was first isolated from rat-tail tendon, has two main α chains (α and α ) and one β chain.
Used as a coating material to support adherent cells growth and differentiation.
Collagen, Type I solution from rat tail has been used for coating culture plates and dishes for human hepatocyte HepG2 cell line and podocytes. It has also been used as a standard for total collagen content calibration curve generation for the quantification of liver extracellular matrix pre-gel.
Biochem/physiol Actions
Collagen is essential for the mechanical integrity of tendons and bone. Rat tail tendon collagen is used in tissue engineering especially in the generation of 3-D scaffolds based gels. It has low antigenicity and is compatible with human gingival fibroblasts and human oral keratinocytes.
Preparation Note
Sterile solution prepared from rat tail tendons by a modification of the published extraction method 1. It is supplied as an aqueous solution in 20mM acetic acid with a protein concentration as stated on the bottle. (approx.100mg protein per vial). Protein concentration was estimated by the Biuret method 2.
SDS polyacrylamide gel electrophoresis analysis shows the typical band pattern for Type I collagen, with a doublet at apparent molecular weights of 115 and 130kDa and another doublet at 215 and 235kDa. Based on this analysis, the purity of the collagen sample is >95%.
Recommended for use as a cell culture substratum. May not be suitable for 3-D gel formation.
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Quality Level	200
biological source	rat tail
description	Approx. 100mg/vial (Volume 22 - 25ml/vial, see label for lot-specific protein concentration)
sterility	sterile-filtered
product line	BioReagent
assay	>95% (SDS-PAGE)
form	liquid
mol wt	apparent mol wt 115-130 kDa by SDS-PAGE (doublet)","apparent mol wt 215-235 kDa by SDS-PAGE (doublet)
packaging	pkg of 1 vial
application(s)	cell culture | mammalian: suitable
surface coverage	610 µg/cm2
UniProt accession no.	P02454,
shipped in	wet ice
storage temp.	2-8°C
Gene Information	rat ... Col1a1(29393)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable