- Производитель:
- Sigma-Aldrich
CAS Number: | 9007-34-5 |
EC Number: | 232-697-4 |
MDL number: | MFCD00130825 |
NACRES: | NA.75 |
Кат. номер |
C5533-5MG-PW |
C5533-5MG |
- To solubilize extracellular matrix for surface functionalization.
- To induce endothelial cell and rat pluripotent cell differentiation.
- As an adherent in cell migration/invasion assay.
- To assess the bioelectric effects of quinine on airway epithelial cells.
- To study the selective toxicity of engineered lentvirus lytic peptides.
- In particle aggregation assay for the rapid detection of fibronectin, fibrinogen and collagen receptors on Staphylococcus aureus.
- In a magnesium-dependent, collagen-binding assay during characterization of human lung tumor-associated antigens.
Biochem/physiol Actions
Type IV collagen maintains cell division and growth. Abnormal collagen secretion affects basement membrane formation. The collagen type IV, 1 (COL4A1) gene is overexpressed in gastric cancer. Mutation in COL4A1 contributes to intracerebral hemorrhage in adults and children. Cerebrovascular disease is resulted due to COL4A1 and COL4A2 mutations. Type IV collagen has been found to play a key role in angiogenesis, neurological diseases and metastasis.
During development, collagen IV is ubiquitously distributed in the basement membranes (BMs). During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining the BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells.
Tissue injury in the autoimmune disease, Goodpasture syndrome, is due to the pathogenic autoantibodies targeting the collagen IV 3 chain. Mutations in the COL4A5 gene are associated with Alport syndrome.
Components
All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type IV collagen occurs only in the basement membranes and contains up to six genetically distinct a-chains.
Caution
This product should be stored desiccated at -20°C, and will retain activity in these conditions for 3 years.
Preparation Note
This is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C. It was prepared by a modification of the pepsin extraction method of Niyibizi, C. et. al
Quality Level | 200 |
biological source | human placenta |
sterility | non-sterile |
product line | BioReagent |
form | powder |
packaging | pkg of 5 mg |
application(s) | cell culture | mammalian: suitable |
impurities | HIV, hepatitis B and hepatitis C, none detected |
solubility | acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.) |
UniProt accession no. | P02462,","P08572,","P29400,","P53420,","Q01955,","Q14031, |
shipped in | wet ice |
storage temp. | 20°C |
Gene Information | human ... COL4A1(1282), COL4A2(1284), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288) |
Personal Protective Equipment | Eyeshields,, Gloves,, type N95 (US), |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point F | Not applicable |
Flash Point C | Not applicable |
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