Chicken Extracellular Chondroitin Sulfate Proteoglycans

eCl@ss:	32160405
NACRES:	NA.75

Кат. номер

CC117

CC117-K

DESCRIPTION/COMPOSITION:CC117 contains a mixture of large, extracellular chondroitin sulfateproteoglycans isolated from embryonic chicken brain. Amino acid sequence analyses and immunological studies have demonstrated that the major components of this mixture are neurocan, phosphacan, versican, and aggrecan. Following chondroitinase treatment to remove the glycosaminoglycan portion of these proteoglycans, the major components of this preparation resolved on SDS gels are 250 kDa core proteins representing neurocan and 400 kDa core proteins representing aggrecan, phosphacan, and versican.
*Quantity: Protein as determined using the bicinchoninic acid (BCA) assay. Note that 100 µg is a measurement of core polypeptides. Components of this mixture contain core polypeptide plus attached glycosaminoglycans
Product Source: Isolated from whole 12-14 day old embryonic chicken brains.
Aggrecan, versican, and neurocan are members of the family of hyaluronic acid-binding proteoglycans and therefore each contains hyaluronic acid binding regions, Ig-like domains, EGF like domains, lectin-like domains, and complement regulatory-like domains. Phosphacan is distinct; it contains a region of similarity to carbonic anhydrase, a fibronectin type III domain, and is a splice variant of the transmembrane protein receptor tyrosine phosphatase equivalent to its extracellular portion.
Although aggrecan was traditionally thought of as a cartilage proteoglycan and neurocan and phosphacan were thought of as neural proteoglycans, recent studies indicate that all four of these proteoglycans have broad yet distinctive distribution patterns inearly embryos. The presence of these proteoglycans has not been carefully examined in adult tissue beyond the expected sites. These proteoglycans are anti-adhesion molecules and are capable of inhibiting both cell-ECM and cell-cell adhesion.
CC117 has been used successfully with rat and mouse brain slice culture systems.
Physical form
Provided as a liquid in PBS, containing no preservatives.
Storage and Stability
Store at -20°C for up to 12 months. Avoid repeated freeze/thaw.
Analysis Note
Mixture of large, extracellular chondroitin sulfate proteoglycans (CSPGs) purified from embryonic chicken brains by chromatrographic methods. By PAGE analysis material is 95% pure, and migrates as a large HMW (<400kDa) mass under reduced conditions. The precise amounts of each CSPG contained in the mixture is not quantified, however the virtually all of the CSPGs present in the source brains are collected.
HOMOGENEITY:
By PAGE analysis material is 95% pure, and migrates as a large HMW (<400kDa) mass under reduced conditions. The precise amounts of CSPG species has not been determined.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	300","100
biological source	chicken
assay	95%
form	liquid
mol wt	<400 kDa
Торговая марка	Chemicon®
concentration	1 mg/mL
application(s)	cell culture | mammalian: suitable
input	sample type mesenchymal stem cell(s) sample type neural stem cell(s) sample type hematopoietic stem cell(s) sample type induced pluripotent stem cell(s) sample type pancreatic stem cell(s) sample type epithelial cells sample type: mouse embryonic stem cell(s)
UniProt accession no.	P07898,","Q90953,","Q9W6E1,
shipped in	dry ice
storage temp.	20°C
Gene Information	chicken ... VCAN(395565)

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable