Puromycin dihydrochloride

CAS Number:	58-58-2
MDL number:	MFCD00012691
PubChem Substance ID:	329820352
NACRES:	NA.85

Кат. номер

P9620-10ML

P9620-10ML-PW

Chemical structure: peptidyl nucleoside
Puromycin is an aminonucleoside antibiotic that is derived from Streptomyces alboniger. It allows selection for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). It has been used to study vascular smooth muscle cell viability after treatment in the rabbit model . Puromycin is used to produce enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer.
10 mL in glass bottle
Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3-terminal end of the aminoacyl-tRNA. Puromycin also acts as a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase.
Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.
Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.
This product is concentrated at 10 mg/mL in water and is 0.2 µm filtered. It has been tested on HeLa cells for cell growth arrest and selection of cells after transfection of the pac resistance gene.
Keep container tightly closed in a dry and well-ventilated place.

Quality Level	300
biological source	Streptomyces alboniger
sterility	0.2 µm filtered
assay	>98% (HPLC)
form	solution
concentration	10 mg/mL in H2O
application(s)	cell culture | mammalian: suitable
solubility	H2O: soluble 10 mg/mL
Mode of action	protein synthesis | interferes
antibiotic activity spectrum	Gram-positive bacteria, neoplastics, parasites
shipped in	wet ice
storage temp.	20°C
SMILES string	Cl.Cl.COc1ccc(C[C@H](N)C(=O)N[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)n3cnc4c(ncnc34)N(C)C)cc1
InChI	1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1
InChI key	MKSVFGKWZLUTTO-FZFAUISWSA-N

Personal Protective Equipment	Eyeshields, Faceshields, Gloves, multi-purpose combination respirator cartridge (US)
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable