Neomycin trisulfate salt hydrate

Empirical Formula (Hill Notation):	C23H46N6O13 · 3H2SO4 · xH2O
CAS Number:	1405-10-3
Molecular Weight:	908.88 (anhydrous basis)
EC Number:	215-773-1
PubChem Substance ID:	24897673
NACRES:	NA.76

Кат. номер

N6386-5G

N6386-100G

N6386-1G

N6386-10G

N6386-25G

Chemical structure: aminoglycoside
Neomycin trisulfate salt hydrate has been used:

• to determine Netrin1- encoding gene (Ntn1) protective effects against aminoglycoside antibiotics in cochlear explants
• for Escherichia coli antibiotic susceptibility test
• in bone marrow transplantation of mice

5, 25, 100 g in glass bottle
Neomycin Trisulfateis an aminoglycoside antibiotic, which is produced by Streptomyces containing a minimum of 85% neomycin B.It is used to study the cytotoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts and extraction of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins. Neomycin trisulfate functions as a selection agent for prokaryotic cells transformed using the neo selectable marker gene. This product is recommended for use in cell culture applications at 5 mL/L.
Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca2+ channels without affecting the Na+/Ca2+ antiporter in neurons.
Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria.
Stock solutions should be filter sterilized and stored at 2-8°C, and are stable at 37°C for 5 days. A 1 mg/mL neomycin solution in .1 M phosphate buffer, pH 8.0 should be used within 14 days when stored at 0-5°C. Solutions should be protected from light or moisture.
Neomycin sulfate is soluble in H2O at 50 mg/mL, yielding a clear solution.
Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place.

biological source	Streptomyces sp.
Quality Level	200,
product line	BioReagent
form	powder
potency	600 µg neomycin per mg (Dried basis)
packaging	pkg of 100 g, pkg of 25 g, pkg of 5 g
application(s)	cell culture | mammalian: suitable
color	white to yellow
solubility	H2O: 50 mg/mL (As a stock solution. Stock solutions should be filter sterilized and stored at 2-8°C. Stable at 37°C for 5 days.)
Mode of action	protein synthesis | interferes
antibiotic activity spectrum	Gram-negative bacteria, Gram-positive bacteria
storage temp.	2-8°C
SMILES string	O.OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.NC[C@@H]1O[C@H](O[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)O[C@@H]3[C@@H](O)[C@H](N)C[C@H](N)[C@H]3O[C@H]4O[C@H](CN)[C@@H](O)[C@H](O)[C@H]4N)C(N)[C@@H](O)[C@@H]1O
InChI	1S/C23H46N6O13.3H2O4S.H2O/c24-2-7-13(32)15(34)10(28)21(37-7)40-18-6(27)1-5(26)12(31)20(18)42-23-17(36)19(9(4-30)39-23)41-22-11(29)16(35)14(33)8(3-25)38-22;3*1-5(2,3)4;/h5-23,30-36H,1-4,24-29H2;3*(H2,1,2,3,4);1H2/t5-,6+,7-,8+,9-,10-,11?,12+,13-,14-,15-,16-,17-,18-,19-,20-,21-,22-,23+;;;;/m1..../s1
InChI key	WHAGUNPVKDUVFV-QGTTWHFQSA-N

pictograms	GHS08
signalword	Danger
hcodes	H317 - H334
pcodes	P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312
Personal Protective Equipment	dust mask type N95 (US),, Eyeshields,, Faceshields,, Gloves,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable