Albumin, Acetylated from bovine serum

CAS Number:	9048-46-8
EC Number:	232-936-2
MDL number:	MFCD01114300
NACRES:	NA.27

Кат. номер

B8894-.1ML

B8894-.1ML-KC

B8894-5ML

B8894-1.5ML

B8894-10ML

B8894-.5ML

Serum albumin is the most extensive plasma protein in the circulatory system. It is produced within the liver and exported as a non-glycosylated protein to the blood plasma. The molecular weight of native serum albumin is around 66 kDa. Native serum albumin is a negatively charged protein.
Acetylation of bovine serum albumin particularly modifies lysine (Lys) residues with elevated frequency, with lesser degrees of modification of serine (Ser) and threonine (Thr) residues. MALDI-TOF mass spectrometry analysis of acetylated BSA has indicated an increased MW of about 3000, compared to native BSA (Ostatn, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. This acetylation process also derivatizes similar amino acid residues in any trace contaminating nucleases in the BSA, to inactivate that nuclease activity. This makes acetylated BSA useful in molecular biology applications where trace nuclease activity must be minimized.
Acetylated BSA has been used as a component of the reaction mixture for adapter ligation. In addition, this B8894 acetylated BSA product has been used in other applications, such as:

• In cDNA library preparation (Hafner, M. et al., RNA, 17(9), 1697-1712 (2011))
• As a blocking agent with streptavidin beads used for capture of biotinylated RNA (Winz, M.-L. et al., Nat. Protocol., 12(1), 122-149 (2017))

Select the right albumin for your application with the Albumin Selection Guide
Acetylated to inactivate nucleases commonly found in BSA. Acetylated BSA should not be used as a protein standard since acetylation of tyrosine residues prevents color development in the Lowry assay and other similar protein determinations.
Biochem/physiol Actions
Native serum albumin plays a major role in maintaining the pH and osmotic pressure of the blood. It is also involved in transporting a diverse range of endogenous and exogenous metabolites, such as amino acids, fatty acids, drugs, steroids and metal ions. Native serum albumin can have a role in modulating the levels of Ca2+ and Mg2+ metals in blood.
Features and Benefits
This acetylated BSA product has been tested for the following properties:

• DNase-free / Exonuclease-free
• Nickase-free / Endonuclease-free
• Protease-free
• RNase-free

Quality Level	200
biological source	bovine
grade	for molecular biology
product line	BioReagent
form	solution
purified by	heat shock fractionation
contains	does not contain stabilizer or preservative
origin	USA origin
concentration	20 mg/mL in H2O
application(s)	FISH: suitable
impurities	HIV I and HIVII, HCV and HBsAg, tested negative
UniProt accession no.	P02769,
foreign activity	DNase free ","DNase, RNase, NICKase and protease, none detected","NICKase free ","Protease free ","RNase free
storage temp.	20°C
Gene Information	bovine ... ALB(280717)

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable