By Ashok Agarwal, Sajal Gupta, Rakesh Sharma
This state of the art laboratory handbook comprises 20 scientific protocols used day-by-day for the research of the infertile male, offered with effortless to appreciate, step by step technique. The protocols are prepared from regimen to complicated laboratory techniques universal to medical perform, together with computer-assisted semen research, sperm education for IUI via density gradient and swim-up, sperm cryopreservation, and sperm DNA fragmentation try through TUNEL strategy, between others. The method in every one protocol follows top perform directions made clearer by way of professionally hand-drawn illustrations masking many of the vital steps and gear. The authors, hailing from the world-renowned Andrology heart at Cleveland medical institution, have over 50 years of mixed first-hand adventure in handling very busy diagnostic and study amenities in male infertility and andrology. The publication may be an crucial source for millions of laboratory technologists, clinicians and reproductive execs (andrologists, embryologist, etc.) engaged within the prognosis and administration of infertile males round the world.
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Additional resources for Andrological Evaluation of Male Infertility: A Laboratory Guide
In the ﬁrst two cases, evaluation of technician performance is indicated. The second case could involve inaccurate dilution of the semen for hemocytometry or inaccurate counting. In the third case, the Makler chambers should be evaluated against a hemocytometer. Z. Drobnis there may be a drift in how morphology is counted by the laboratory’s technicians . The SOP for each control procedure should include a list of the steps to be taken in the event of each anticipated detection of systematic error.
Semen parameters, including WHO and strict criteria morphology, in a fertile and subfertile population: an effort towards standardization of in-vivo thresholds. Hum Reprod. 2001;16:1165–71. 32. Sergerie M, Laforest G, Bujan L, Bissonnette F, Bleau G. Sperm DNA fragmentation: threshold value in male fertility. Hum Reprod. 2005;20:3446–51. 33. Simon L, Lutton D, McManus J, Lewis SEM. Sperm DNA damage measured by the alkaline Comet assay as an independent predictor of male infertility and in vitro fertilization success.
If computer-assisted sperm analysis (CASA) is used by the laboratory to evaluate sperm motility in patient specimens, then videotapes should be used as a routine control of the CASA determinations. 5 Fig. 2 Levey–Jennings charts showing daily counts of sperm-sized latex bead at two concentrations in a single Makler chamber. (a) High bead concentration and (b) low bead concentration. The expected value (EV), taken as the mean value for the 100 days, is shown as a horizontal black line. The purple lines show one standard deviation (SD) from the EV, the orange lines indicate 2 SD from the EV, and the green lines indicate 3 SD from the EV.
Andrological Evaluation of Male Infertility: A Laboratory Guide by Ashok Agarwal, Sajal Gupta, Rakesh Sharma