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Extra info for Acute toxicology
The test substance skin site is wiped to remove any test substance still remaining. When colored test substances (such as dyes) are used, it may be necessary to wash the test substance from the test site with an appropriate solvent or vehicle (one that is suitable for the substance being tested). This is done to clean the test site to facilitate accurate evaluation for skin irritation. Immediately after removal of the patches, each 1 • 1-in. square test or control site is outlined with an indelible marker by dotting each of the four corners.
And Hart, J. B. (1987). Prediction of rabbit skin irritation severity by structure-activity relationships. In Vitro Toxicol. 1, 129-147. Environmental Protection Agency (EPA) (1979). Acute Toxicity Testing Criteria for New Chemical Substances (EPA 560/13-79-009) EPA, Office of Toxic Substances, Washington, DC. Firestone, B. , and Guy, R. H. (1986). Approaches to the prediction of dermal absorption and potential cutaneous toxicity. In Alternative Methods in Toxicology (A. M. ), Vol. 3, pp. 56-536.
The test substance patch is placed on skin site 1 and secured. a. 5 g. b. , the gauze patch designated for the test sample is secured to the appropriate skin site with one of four strips of tape at the most central position of the animal. 5 ml is carefully poured from a glycine weighing paper onto the gauze patch, which is held in a horizontal (level) position with the other hand. The patch containing the test sample is then carefully placed into position onto the skin by raising the remaining three edges dorsally until they are completely secured with tape.